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    • 2. 发明授权
      US06225061B1 Systems and methods for performing reactions in an unsealed environment 有权
    • Systems and methods for performing reactions in an unsealed environment
    • 在非密封环境中进行反应的系统和方法
    • US06225061B1
    • 2001-05-01
    • US09266409
    • 1999-03-10
    • Thomas Becker , Hubert Köster , Charles Cantor
    • Thomas Becker , Hubert Köster , Charles Cantor
    • C12Q168
    • B01J19/16 , B01J2219/00317 , B01J2219/00367 , B01J2219/00378 , B01J2219/00495 , B01J2219/00603 , B01J2219/0068 , C40B60/14 , G01N2035/00287 , Y10T436/11 , Y10T436/25375
    • An open system is provided for performing a submicroliter reaction. An open system can contain a solid support having a target site for performing the reaction; a liquid dispensing system such as a nanoliter dispensing pipette for dispensing a submicroliter amount of a liquid to the target site; a temperature controlling device for regulating the temperature of the support; and means for controlling the amount of liquid dispensed, which corresponds to the amount of liquid that evaporates from the target site. Also provided is an open system, including a solid support having a target site; a liquid dispensing system, which can dispense a liquid to the target site; a temperature controlling system, which regulates the temperature of the solid support; and an interface, which regulates an amount of liquid dispensed from the liquid dispensing system. Also provided is a method for performing a reaction in a submicroliter volume in an unsealed environment by dispensing a submicroliter volume of liquid onto the surface of a support; monitoring the temperature of the support; monitoring an amount or rate of evaporation of the liquid; and dispensing to the surface of the support a further amount of the liquid, which corresponds to the amount lost from the support due to evaporation, thereby maintaining the reaction volume at a predetermined volume throughout the course of the reaction. A method also is provided for maintaining a volume of a reaction mixture, which can be one of a plurality of reaction mixtures, on a solid support in an unsealed environment by monitoring the rate of evaporation of a liquid from the reaction mixture; and dispensing into the reaction mixture an amount of liquid that corresponds to the amount that evaporates.
    • 提供了用于进行亚微米级反应的开放系统。 开放系统可以含有具有用于进行反应的靶位点的固体支持物; 液体分配系统，例如用于将目标部位的微量剂量的液体分配的纳升分配移液管; 用于调节支撑件的温度的温度控制装置; 以及用于控制分配的液体量的装置，其对应于从目标部位蒸发的液体的量。 还提供了一种开放系统，包括具有目标地点的固体支撑物; 液体分配系统，其可以将液体分配到目标部位; 温度控制系统，其调节固体支持物的温度; 以及界面，其调节从液体分配系统分配的液体的量。 还提供了一种在非密封环境中通过将亚微米级体积的液体分配到载体的表面上来进行亚微米体积的反应的方法; 监测支架的温度; 监测液体的蒸发量或速率; 并且向支撑体的表面分配更多量的液体，其对应于由于蒸发而从支撑物损失的量，从而在反应过程中将反应体积保持在预定体积。 还提供了一种方法，用于通过监测来自反应混合物的液体的蒸发速率来保持反应混合物的体积，反应混合物可以是多个反应混合物中的一种，在固体支持物上在未密封环境中; 并向反应混合物中分配一定量的与蒸发量对应的液体。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
    • 3. 发明授权
      US07803529B1 Solid phase sequencing of biopolymers 有权
    • Solid phase sequencing of biopolymers
    • 生物聚合物的固相测序
    • US07803529B1
    • 2010-09-28
    • US09395409
    • 1999-09-14
    • Charles Cantor , Hubert Köster
    • Charles Cantor , Hubert Köster
    • C12Q1/68
    • C12Q1/6872 , C12Q1/6874 , C12Q2565/501 , C12Q2531/113 , C12Q2521/525 , C12Q2565/627 , C12Q2531/119 , C12Q2523/301 , C12Q2531/137 , C12Q2521/301 , C12Q2531/149
    • This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
    • 本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 可以确定其序列的核酸包括生物样品中的DNA或RNA，例如患者活组织检查和环境样品。 探针可以固定在固体支持物例如杂交芯片上以促进自动化分子量分析和靶序列的鉴定。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
    • 5. 发明申请
      US20110172111A1 SOLID PHASE SEQUENCING OF BIOPOLYMERS 失效
    • SOLID PHASE SEQUENCING OF BIOPOLYMERS
    • 生物聚合物的固相序列
    • US20110172111A1
    • 2011-07-14
    • US12852336
    • 2010-08-06
    • Charles Cantor , Hubert Köster
    • Charles Cantor , Hubert Köster
    • C40B30/04
    • C12Q1/6872 , C12Q1/6874 , C12Q2565/501 , C12Q2531/113 , C12Q2521/525 , C12Q2565/627 , C12Q2531/119 , C12Q2523/301 , C12Q2531/137 , C12Q2521/301 , C12Q2531/149
    • This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
    • 本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 可以确定其序列的核酸包括生物样品中的DNA或RNA，例如患者活组织检查和环境样品。 探针可以固定在固体支持物例如杂交芯片上以促进自动化分子量分析和靶序列的鉴定。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
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