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    • 5. 发明申请
    • ELECTRO-DIFFUSION ENHANCED BIO-MOLECULE CHARGE DETECTION USING ELECTROSTATIC INTERACTION
    • 使用静电相互作用的电扩散增强的生物分子电荷检测
    • US20170074854A1
    • 2017-03-16
    • US15271014
    • 2016-09-20
    • The Board of Trustees of the Leland Stanford Junior University
    • Yang LiuRobert W. DuttonRoger T. Howe
    • G01N33/487G01N27/414G01N27/447
    • G01N33/48721C12Q1/68G01N27/04G01N27/4145G01N27/44791G01N33/68
    • According to one aspect, the disclosure is directed to an example embodiment in which a circuit-based arrangement includes a circuit-based substrate securing a channel, with an effective width that is not limited by the Debye screening length, along a surface of the substrate. A pair of reservoirs are included in or on the substrate and configured for containing and presenting a sample having bio-molecules for delivery in the channel. A pair of electrodes electrically couple a charge in the sample to enhance ionic current flow therein (e.g., to overcome the electrolyte screening), and a sense electrode is located along the channel for sensing a characteristic of the biological sample by using the electrostatic interaction between the enhanced ionic current flow of the sample and the sense electrode. Actual detection occurs by using a charge-signal processing circuit to process the sensed charge signal and, therefrom, provide an output indicative of a signature for the bio-molecules delivered in the channel.
    • 根据一个方面,本发明涉及一个示例性实施例,其中基于电路的布置包括基于电路的基板,其沿着基板的表面固定具有不受德拜屏蔽长度限制的有效宽度的通道 。 一对储存器包含在基底中或基底上,并且被配置为容纳和呈现具有用于在通道中递送的生物分子的样品。 一对电极将样品中的电荷电耦合以增强其中的离子电流(例如,以克服电解质屏蔽),并且感测电极沿着通道定位,以通过使用 样品和感应电极的增强的离子电流流动。 通过使用电荷信号处理电路来处理所感测的电荷信号,从而提供指示在通道中递送的生物分子的签名的输出,发生实际检测。