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1. 发明申请

US20080299541A1 METHOD AND APPARATUS FOR MEASURING ENZYMATIC ACTIVITY BY USE OF LASER 失效
标题翻译：通过使用激光测量酶活性的方法和装置
公开(公告)号：US20080299541A1
公开(公告)日：2008-12-04
申请号：US12110969
申请日：2008-04-28
申请人： Takuya Kishimoto , Hidetoshi Watanabe , Michihiro Ohnishi
发明人： Takuya Kishimoto , Hidetoshi Watanabe , Michihiro Ohnishi
IPC分类号： C12Q1/00 , C12M1/00
CPC分类号： A61B5/0059 , A61B5/0071 , A61B5/441
摘要： A method of measuring an enzymatic activity, includes measuring the quantity of a substrate metabolite produced upon metabolism of a substrate by an enzyme, through detecting a radiant wave generated from a multiple photon excitation process of the substrate or the substrate metabolite.
摘要翻译：测量酶活性的方法包括通过检测由底物或底物代谢物的多光子激发过程产生的辐射波来测量由酶代谢基质时产生的底物代谢物的量。

2. 发明申请

US20090324498A1 METHOD OF MEASURING QUANTITY OF IN-VIVO SUBSTANCE BY USE OF COHERENT ANTI-STOKES RAMAN SCATTERED LIGHT 审中-公开
标题翻译：通过使用相同的反坦克拉曼散射光来测量体内物质的数量的方法
公开(公告)号：US20090324498A1
公开(公告)日：2009-12-31
申请号：US12486290
申请日：2009-06-17
申请人： Takuya Kishimoto , Michihiro Ohnishi , Hidetoshi Watanabe
发明人： Takuya Kishimoto , Michihiro Ohnishi , Hidetoshi Watanabe
IPC分类号： A61K49/00 , C12Q1/02
CPC分类号： G01N21/65 , A61B5/14546 , A61B5/1455 , G01N2021/653 , G01N2021/656
摘要： A method of measuring the quantity of an in-vivo substance includes the steps of: irradiating an in-vivo substance with two near infrared femtosecond laser beams of different frequencies; detecting coherent anti-Stokes Raman scattered light emitted from the in-vivo substance due to coincidence of the frequency difference between the two near infrared femtosecond laser beams with a natural frequency of the in-vivo substance; and determining the quantity of the in-vivo substance, based on a peak intensity of a Raman scattering spectrum obtained.
摘要翻译：测定体内物质量的方法包括以下步骤：用两个不同频率的近红外飞秒激光照射体内物质; 由体内物质的固有频率引起的两个近红外飞秒激光束之间的频率差的一致性检测从体内物质发射的相干反斯托克斯拉曼散射光; 并且基于所获得的拉曼散射光谱的峰值强度来确定体内物质的量。

3. 发明申请

US20090275743A1 METHOD OF PREPARING CARRIER TO SEPARATE NUCLEIC ACIDS, CARRIER AND MICRO CHANNEL TO SEPARATE NUCLEIC ACIDS, AND METHOD AND APPARATUS FOR SEPARATING NUCLEIC ACIDS 有权
标题翻译：制备分离核酸的载体的方法，分离核酸的载体和微通道的方法以及分离核酸的方法和装置
公开(公告)号：US20090275743A1
公开(公告)日：2009-11-05
申请号：US12432078
申请日：2009-04-29
申请人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
发明人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
IPC分类号： C07H1/00 , B01J19/00 , B05D3/00
CPC分类号： C12N15/1006
摘要： The present invention provides a method for producing a carrier for nucleic acid separation, the method including a step of: immobilizing capturing chains onto a porous carrier having cation-exchangeable groups thereon by ion exchange bonding, the capturing chains having a base sequence complementary to target nucleic acid chains and being modified with functional groups having positive charges.
摘要翻译：本发明提供了一种核酸分离用载体的制造方法，该方法包括以下步骤：通过离子交换键将捕获链固定在其上具有阳离子交换基团的多孔载体上，捕获链具有与靶相互补充的碱基序列核酸链，并用具有正电荷的官能团修饰。

4. 发明授权

US08785348B2 Method of preparing carrier to separate nucleic acids, carrier and micro channel to separate nucleic acids, and method and apparatus for separating nucleic acids 有权
标题翻译：制备分离核酸，载体和微通道以分离核酸的载体的方法，以及用于分离核酸的方法和装置
公开(公告)号：US08785348B2
公开(公告)日：2014-07-22
申请号：US12432078
申请日：2009-04-29
申请人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
发明人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
IPC分类号： B01J20/00 , B01J23/02 , C07H21/00
CPC分类号： C12N15/1006
摘要： The present disclosure relates to a method and apparatus for separating nucleic acids. A carrier may include a porous microbead having cation-exchangeable groups attached to the surface of the porous microbead. Capturing chains modified with positively charged functional groups and having a base sequence complementary to a target nucleic acid chain sequence are immobilized on to the surface of the porous microbead. In various embodiments, capturing chains are immobilized on to the surface of the porous microbead through an ion exchange bond or a covalent bond with the cation-exchangeable groups of the porous microbead. In some cases, the porous microbead has a number of through pores adapted to permit a solution to pass rapidly through the through pores and a number of diffusive pores adapted to permit a solute of the solution to diffuse into the diffusive pores. A solution having a target nucleic acid chain corresponding to the target nucleic acid chain sequence of the capturing chains may be passed through a channel that is packed with porous microbeads so as to result in hybridization of the capturing chain and the target nucleic acid chain. The porous microbeads may be structured such that the pressure in the channel upon passing of the solution through the channel does not build significantly.
摘要翻译：本公开涉及用于分离核酸的方法和装置。载体可以包括具有连接到多孔微珠表面的可阳离子交换基团的多孔微珠。用正电荷的官能团修饰并具有与靶核酸链序列互补的碱基序列的捕获链被固定在多孔微珠的表面上。在各种实施方案中，捕获链通过与多孔微珠的可阳离子交换基团的离子交换键或共价键固定在多孔微珠的表面上。在一些情况下，多孔微珠具有适于使溶液快速通过通孔的多个通孔，并且适于允许溶液的溶质扩散到扩散孔中的许多扩散孔。具有对应于捕获链的靶核酸链序列的靶核酸链的溶液可以通过填充有多孔微珠的通道，从而导致捕获链和靶核酸链的杂交。多孔微珠可以被构造成使得溶液通过通道时通道中的压力不会显着增加。

5. 发明申请

US20120010097A1 MICROCHANNEL, AND NUCLEIC ACID HYBRIDIZATION MICROCHIP, COLUMN, SYSTEM AND METHOD 审中-公开
标题翻译：微通道和核酸混合微孔，柱，系统和方法
公开(公告)号：US20120010097A1
公开(公告)日：2012-01-12
申请号：US13171851
申请日：2011-06-29
申请人： Michihiro Ohnishi , Naoyuki Sasaki , Takuya Kishimoto , Hidetoshi Watanabe
发明人： Michihiro Ohnishi , Naoyuki Sasaki , Takuya Kishimoto , Hidetoshi Watanabe
IPC分类号： C40B30/04 , C40B40/06 , B01D35/00
CPC分类号： C12Q1/6813 , C12Q2527/101 , C12Q2565/629
摘要： A microchannel capable of passing therethrough a solution with target nucleic acid strands contained therein includes: an agarose gel carrier having light transmission properties, supporting capture strands having a base sequence complementary to that of the target nucleic acid strands and immobilized on the agarose gel carrier, and packed in the microchannel; and a filter arranged in the microchannel on a downstream side as viewed in a passing direction of the solution and retaining the agarose gel carrier.
摘要翻译：能够通过其中含有目标核酸链的溶液的微通道包括：具有透光性的琼脂糖凝胶载体，支持具有与靶核酸链互补的碱基序列并固定在琼脂糖凝胶载体上的捕获链，并包装在微通道中; 以及在溶液的通过方向上观察的下游侧的微通道中布置的过滤器，并保持琼脂糖凝胶载体。

6. 发明授权

US08030018B2 Method for measuring enzymatic activity in vivo by use of laser 失效
标题翻译：通过使用激光测量体内酶活性的方法
公开(公告)号：US08030018B2
公开(公告)日：2011-10-04
申请号：US12110969
申请日：2008-04-28
申请人： Tauya Kishimoto , Hidetoshi Watanabe , Michihiro Ohnishi
发明人： Tauya Kishimoto , Hidetoshi Watanabe , Michihiro Ohnishi
IPC分类号： C12Q1/26
CPC分类号： A61B5/0059 , A61B5/0071 , A61B5/441
摘要： A method of measuring an enzymatic activity, includes measuring the quantity of a substrate metabolite produced upon metabolism of a substrate by an enzyme, through detecting a radiant wave generated from a multiple photon excitation process of the substrate or the substrate metabolite.
摘要翻译：测量酶活性的方法包括通过检测由底物或底物代谢物的多光子激发过程产生的辐射波来测量由酶代谢基质时产生的底物代谢物的量。

7. 发明申请

US20130020201A1 NUCLEIC-ACID EXTRACTION METHOD AND NUCLEIC-ACID EXTRACTION CARTRIDGE 有权
标题翻译：核酸提取方法和核酸提取盒
公开(公告)号：US20130020201A1
公开(公告)日：2013-01-24
申请号：US13533348
申请日：2012-06-26
申请人： Tasuku Yotoriyama , Tomohiko Nakamura , Naohisa Sakamoto , Kazumine Ito , Tomoteru Abe , Michihiro Ohnishi
发明人： Tasuku Yotoriyama , Tomohiko Nakamura , Naohisa Sakamoto , Kazumine Ito , Tomoteru Abe , Michihiro Ohnishi
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： C12N15/1006 , C12N15/101
摘要： The present disclosure provides a nucleic-acid extraction method for carrying out the following procedures in the same cell, the procedures including: performing an ultrasonic process on a sample containing a nucleic acid; adsorbing substances contained in the sample by making use of an adsorption carrier; and condensing the nucleic acid by damming the nucleic acid moving in an electrophoresis phenomenon. In accordance with the nucleic-acid extraction method, it is possible to carry out an ultrasonic process, an adsorption process and an electrophoresis process in the same cell. Thus, it is possible to eliminate an operation to transfer the sample from one cell to another one for each of the processes.
摘要翻译：本公开内容提供了用于在相同细胞中进行以下程序的核酸提取方法，其方法包括：对含有核酸的样品进行超声波处理; 通过使用吸附载体吸附样品中所含的物质; 并通过阻止以电泳现象移动的核酸来冷凝核酸。根据核酸提取方法，可以在同一电池中进行超声波处理，吸附处理和电泳处理。因此，可以消除用于每个处理将样品从一个单元传送到另一个单元的操作。

8. 发明申请

US20060216816A1 Flow path system provided with reaction section suited for the detection of hybridization, and hybridization detecting device making use of the flow path system 审中-公开
标题翻译：提供了适用于检测杂交的反应段的流路系统，以及利用流路系统的杂交检测装置
公开(公告)号：US20060216816A1
公开(公告)日：2006-09-28
申请号：US11378361
申请日：2006-03-20
申请人： Michihiro Ohnishi
发明人： Michihiro Ohnishi
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01L3/502746 , B01L3/561 , B01L2300/0681 , B01L2300/0838 , B01L2400/0487 , B01L2400/084 , C12Q1/6837 , G01N30/02 , B01D15/345
摘要： A flow path system suitable for use in the detection of hybridization includes a capillary provided at a predetermined location thereof with a reaction section packed with beads. On the beads, a linker having a sequence of bases of the same type is immobilized, and a target nucleic acid complementarily bound to the sequence of the bases of the same type is held.
摘要翻译：适用于检测杂交的流路系统包括在其预定位置处提供的填充有珠粒的反应段的毛细管。在珠粒上，固定具有相同碱基序列的接头，并保持与相同碱基序列互补结合的靶核酸。

9. 发明授权

US07035008B2 Screen, its manufacturing method and image display system 失效
标题翻译：屏幕，其制造方法和图像显示系统
公开(公告)号：US07035008B2
公开(公告)日：2006-04-25
申请号：US11103212
申请日：2005-04-11
申请人： Atsushi Toda , Yoshihiko Kuroki , Michihiro Ohnishi , Hirotaka Akao , Ko Ishimoto
发明人： Atsushi Toda , Yoshihiko Kuroki , Michihiro Ohnishi , Hirotaka Akao , Ko Ishimoto
IPC分类号： G03B21/56 , G03B21/60
CPC分类号： G03B21/567 , B82Y20/00 , G02B1/005 , G02B5/003 , G02B5/26 , G02F2201/50 , G02F2202/32 , G03B21/56 , G03B21/60
摘要： A projection screen comprises a red-reflecting particle layer, green-reflecting particle layer and blue-reflecting particle layer sequentially stacked on a substrate. In each particle layer particles are accumulated by eleven cycles in a regularly alignment such as close-packed structure. Diameter of red-reflecting particles is approximately 280 nm, diameter of green-reflecting particles is approximately 235 nm, and diameter of blue-reflecting particles is approximately 212 nm. Each particles layer is accumulated by self-organized technique. The substrate used here can absorb light of wavelengths other than those of red, green and blue three primary colors.
摘要翻译：投影屏幕包括依次堆叠在基板上的红色反射粒子层，绿色反射粒子层和蓝色反射粒子层。在每个颗粒层中，颗粒以规则对准如紧密堆积的结构积累十一个周期。红色反射粒子的直径约为280nm，绿色反射粒子的直径约为235nm，蓝色反射粒子的直径约为212nm。每个颗粒层通过自组织技术积累。这里使用的基板可以吸收不同于红，绿，蓝三原色波长的光。

10. 发明授权

US06674561B2 Optical state modulation method and system, and optical state modulation apparatus 失效
标题翻译：光学状态调制方法和系统以及光学状态调制装置
公开(公告)号：US06674561B2
公开(公告)日：2004-01-06
申请号：US10085533
申请日：2002-02-26
申请人： Michihiro Ohnishi , Tomohiro Nishi
发明人： Michihiro Ohnishi , Tomohiro Nishi
IPC分类号： G02F101
CPC分类号： H04N5/74 , H04N5/913 , H04N2005/91392
摘要： An optical state modulation method is provided. The method comprises steps of periodically modulating luminance of a visible light in temporal domain so as to generate an optical state variation on a recorded image that is obtained by image-capturing of a displayed image, the visible light being superposed on an original display image to produce the displayed image, the optical state variation being independent of an original display image and generating no hampering effect when the displayed image is directly watched.
摘要翻译：提供了一种光学状态调制方法。该方法包括以下步骤：周期地调制时域中的可见光的亮度，以便通过对所显示的图像进行图像捕获而获得的记录图像产生光学状态变化，将可见光叠加在原始显示图像上产生显示的图像，光学状态变化与原始显示图像无关，并且当直接观看所显示的图像时不产生阻碍效果。

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