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1. 发明授权

US08785348B2 Method of preparing carrier to separate nucleic acids, carrier and micro channel to separate nucleic acids, and method and apparatus for separating nucleic acids 有权
标题翻译：制备分离核酸，载体和微通道以分离核酸的载体的方法，以及用于分离核酸的方法和装置
公开(公告)号：US08785348B2
公开(公告)日：2014-07-22
申请号：US12432078
申请日：2009-04-29
申请人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
发明人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
IPC分类号： B01J20/00 , B01J23/02 , C07H21/00
CPC分类号： C12N15/1006
摘要： The present disclosure relates to a method and apparatus for separating nucleic acids. A carrier may include a porous microbead having cation-exchangeable groups attached to the surface of the porous microbead. Capturing chains modified with positively charged functional groups and having a base sequence complementary to a target nucleic acid chain sequence are immobilized on to the surface of the porous microbead. In various embodiments, capturing chains are immobilized on to the surface of the porous microbead through an ion exchange bond or a covalent bond with the cation-exchangeable groups of the porous microbead. In some cases, the porous microbead has a number of through pores adapted to permit a solution to pass rapidly through the through pores and a number of diffusive pores adapted to permit a solute of the solution to diffuse into the diffusive pores. A solution having a target nucleic acid chain corresponding to the target nucleic acid chain sequence of the capturing chains may be passed through a channel that is packed with porous microbeads so as to result in hybridization of the capturing chain and the target nucleic acid chain. The porous microbeads may be structured such that the pressure in the channel upon passing of the solution through the channel does not build significantly.
摘要翻译：本公开涉及用于分离核酸的方法和装置。载体可以包括具有连接到多孔微珠表面的可阳离子交换基团的多孔微珠。用正电荷的官能团修饰并具有与靶核酸链序列互补的碱基序列的捕获链被固定在多孔微珠的表面上。在各种实施方案中，捕获链通过与多孔微珠的可阳离子交换基团的离子交换键或共价键固定在多孔微珠的表面上。在一些情况下，多孔微珠具有适于使溶液快速通过通孔的多个通孔，并且适于允许溶液的溶质扩散到扩散孔中的许多扩散孔。具有对应于捕获链的靶核酸链序列的靶核酸链的溶液可以通过填充有多孔微珠的通道，从而导致捕获链和靶核酸链的杂交。多孔微珠可以被构造成使得溶液通过通道时通道中的压力不会显着增加。

2. 发明申请

US20090275743A1 METHOD OF PREPARING CARRIER TO SEPARATE NUCLEIC ACIDS, CARRIER AND MICRO CHANNEL TO SEPARATE NUCLEIC ACIDS, AND METHOD AND APPARATUS FOR SEPARATING NUCLEIC ACIDS 有权
标题翻译：制备分离核酸的载体的方法，分离核酸的载体和微通道的方法以及分离核酸的方法和装置
公开(公告)号：US20090275743A1
公开(公告)日：2009-11-05
申请号：US12432078
申请日：2009-04-29
申请人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
发明人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
IPC分类号： C07H1/00 , B01J19/00 , B05D3/00
CPC分类号： C12N15/1006
摘要： The present invention provides a method for producing a carrier for nucleic acid separation, the method including a step of: immobilizing capturing chains onto a porous carrier having cation-exchangeable groups thereon by ion exchange bonding, the capturing chains having a base sequence complementary to target nucleic acid chains and being modified with functional groups having positive charges.
摘要翻译：本发明提供了一种核酸分离用载体的制造方法，该方法包括以下步骤：通过离子交换键将捕获链固定在其上具有阳离子交换基团的多孔载体上，捕获链具有与靶相互补充的碱基序列核酸链，并用具有正电荷的官能团修饰。

3. 发明申请

US20090137053A1 BEAD SET, PRODUCTION PROCESS OF THE BEAD SET, AND METHOD OF USING THE BEAD SET 审中-公开
标题翻译：珠组，珠组的生产过程以及使用珠组的方法
公开(公告)号：US20090137053A1
公开(公告)日：2009-05-28
申请号：US12108348
申请日：2008-04-23
申请人： Noriyuki Kishii , Mari Ichimura , Tomoteru Abe , Kazuhiro Nakagawa , Akio Yasuda , Mayumi Shiono , Michihiro Ohnishi , Takuro Yamamoto , Minoru Takeda
发明人： Noriyuki Kishii , Mari Ichimura , Tomoteru Abe , Kazuhiro Nakagawa , Akio Yasuda , Mayumi Shiono , Michihiro Ohnishi , Takuro Yamamoto , Minoru Takeda
IPC分类号： G01N33/50 , B05D3/00
CPC分类号： G01N21/645 , C12Q1/6869 , G01N33/54313 , Y10T436/143333 , C12Q2563/149
摘要： A bead set is composed of plural subsets of beads. The beads in each subset are each provided with a surface permitting immobilization thereon of a substance that takes part in a predetermined corresponding reaction or interaction. The individual beads in the beat set are provided with different physical elements, respectively, such that the individual beads in the bead can be distinguished into the plural subsets through an analysis of captured images of the individual beads in the bead set by a computer.
摘要翻译：珠组由多个珠子组成。每个子集中的珠粒各自具有允许在其上固定参与预定相应反应或相互作用的物质的表面。节拍组中的各个珠分别设置有不同的物理元件，使得可以通过分析由计算机设置的珠粒中的各个珠子的捕获图像来将珠粒中的各个珠子区分为多个子集。

4. 发明授权

US08753898B2 Microbead analysis method and microbead analyzer 失效
标题翻译：微珠分析法和微珠分析仪
公开(公告)号：US08753898B2
公开(公告)日：2014-06-17
申请号：US12877237
申请日：2010-09-08
申请人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
发明人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
IPC分类号： G01N33/543
CPC分类号： G01N33/54366 , G01N21/6452
摘要： Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.
摘要翻译：微珠的微珠分析方法。微珠形成为具有彼此面对的顶表面和底表面的几乎平行的柱状和从其延伸的侧表面，并且携带形成在顶表面和底部中的至少一个上的识别图案表面和固定在其对分析物质具有亲和性的表面上的物质。该方法包括检测由于分析物质与对分析物质具有亲和性的物质的相互作用而从微珠表面发射的荧光，该区域包括不形成识别图案的顶表面和底表面的区域，侧面表面。

5. 发明申请

US20110136263A1 MICROBEAD ANALYSIS METHOD AND MICROBEAD ANALYZER 失效
标题翻译： MICROBEAD分析方法和MICROBEAD分析仪
公开(公告)号：US20110136263A1
公开(公告)日：2011-06-09
申请号：US12877237
申请日：2010-09-08
申请人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
发明人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
IPC分类号： G01N33/543 , G01N21/64
CPC分类号： G01N33/54366 , G01N21/6452
摘要： Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.
摘要翻译：微珠的微珠分析方法。微珠形成为具有彼此面对的顶表面和底表面的几乎平行的柱状和从其延伸的侧表面，并且携带形成在顶表面和底部中的至少一个上的识别图案表面和固定在其对分析物质具有亲和性的表面上的物质。该方法包括检测由于分析物质与对分析物质具有亲和性的物质的相互作用而从微珠表面发射的荧光，该区域包括不形成识别图案的顶表面和底表面的区域，侧面表面。

6. 发明申请

US20110196619A1 METHOD FOR PREDICTING BIOLOGICAL RHYTHM 审中-公开
标题翻译：预测生物RHYTHM的方法
公开(公告)号：US20110196619A1
公开(公告)日：2011-08-11
申请号：US13123847
申请日：2009-10-13
申请人： Haruhiko Soma , Takuro Yamamoto , Makoto Akashi , Koichi Node , Noriyuki Kishii , Kazuhiro Nakagawa , Shiko Yamashita , Tomohiro Hayakawa
发明人： Haruhiko Soma , Takuro Yamamoto , Makoto Akashi , Koichi Node , Noriyuki Kishii , Kazuhiro Nakagawa , Shiko Yamashita , Tomohiro Hayakawa
IPC分类号： G06F19/00
CPC分类号： C12Q1/6876 , A61B10/02 , C12Q1/6809 , C12Q2600/158 , G16B25/00 , G16B40/00 , C12Q2537/165
摘要： There is provided a method that achieves the prediction of a biological rhythm with high precision while minimizing the number of times a biological specimen is collected from a subject. There is also provided a method for predicting a biological rhythm of a subject on the basis of time series expression level data obtained by measuring expression levels of two clock genes in biological specimens collected from the subject three times within 24 hours, the clock genes having different phases of circadian cycles of a change in expression levels. In this method for predicting a biological rhythm, a biological rhythm can be predicted with particularly high precision by collecting a biological specimen from a subject three times within 24 hours at eight-hour intervals.
摘要翻译：提供了一种以最小化从受试者收集生物标本的次数来实现高精度的生物节律预测的方法。还提供了一种基于通过在24小时内测量从受试者收集的生物标本中的两个时钟基因的表达水平获得的时间序列表达水平数据，时钟基因具有不同的方式来预测受试者的生物节律的方法表达水平发生变化的昼夜循环阶段。在这种用于预测生物节律的方法中，通过以8小时的间隔在24小时内收集受试者三次生物样本，可以以特别高的精度预测生物节律。

7. 发明申请

US20080220509A1 Nucleic Acid Amplifier 有权
标题翻译：核酸放大器
公开(公告)号：US20080220509A1
公开(公告)日：2008-09-11
申请号：US12037619
申请日：2008-02-26
申请人： Yuji SEGAWA , Tasuku Yotoriyama , Akio Yasuda , Noriyuki Kishii , Mayumi Shiono , Takuro Yamamoto , Tomoteru Abe
发明人： Yuji SEGAWA , Tasuku Yotoriyama , Akio Yasuda , Noriyuki Kishii , Mayumi Shiono , Takuro Yamamoto , Tomoteru Abe
IPC分类号： C12M1/00
CPC分类号： B01L7/00 , B01L3/50851 , B01L7/54 , B01L2300/0654 , B01L2300/0819 , B01L2300/1822 , B01L2300/1827 , G01N21/0332 , G01N21/6428 , G01N21/6454 , G01N2021/0325 , G01N2201/062
摘要： Disclosed herein is a nucleic acid amplifier for carrying out a nucleic acid amplification reaction, the amplifier including at least: a plurality of wells configured to carry out the nucleic acid amplification reaction; a heating unit provided for every well; optical means capable of irradiating excitation light of a specified wavelength to all of the wells; and a fluorescence detection unit provided for every well.
摘要翻译：本文公开了一种用于进行核酸扩增反应的核酸放大器，所述放大器至少包括：构造成进行核酸扩增反应的多个阱; 为每个井提供加热单元; 能够将特定波长的激发光照射到所有孔的光学装置; 以及为每个孔提供的荧光检测单元。

8. 发明授权

US06995116B2 Reversible multicolor recording medium, and recording method using the same 失效
标题翻译：可逆多色记录介质及使用其的记录方法
公开(公告)号：US06995116B2
公开(公告)日：2006-02-07
申请号：US10645168
申请日：2003-08-19
申请人： Hisanori Tsuboi , Noriyuki Kishii , Kenichi Kurihara
发明人： Hisanori Tsuboi , Noriyuki Kishii , Kenichi Kurihara
IPC分类号： B41M5/30
CPC分类号： B41M5/305 , B41M5/34 , G11B2007/24618 , Y10T428/24802
摘要： A reversible multicolor recording medium includes a supporting substrate, and recording layers which have, respectively, a plurality of reversible thermal coloring compositions having different colors. The recording layers are separated from and stacked on one another on the supporting substrate. The reversible thermal coloring compositions respectively include light-to-heat transforming materials which respectively absorb infrared rays having different wavelength ranges to generate heat.
摘要翻译：可逆的多色记录介质包括支撑基底和分别具有多种具有不同颜色的可逆热着色组合物的记录层。记录层在支撑基板上彼此分离并堆叠。可逆热着色组合物分别包括分别吸收具有不同波长范围的红外线产生热量的光热转换材料。

9. 发明申请

US20050225891A1 Reversible multicolor recording medium and recording method using it 失效
标题翻译：可逆多色记录介质和使用它的记录方法
公开(公告)号：US20050225891A1
公开(公告)日：2005-10-13
申请号：US10531339
申请日：2003-10-07
申请人： Hisanori Tsuboi , Noriyuki Kishii , Kenichi Kurihara
发明人： Hisanori Tsuboi , Noriyuki Kishii , Kenichi Kurihara
IPC分类号： B42D15/10 , B41J2/32 , B41M5/28 , B41M5/30 , B41M5/323 , B41M5/337 , B41M5/34 , B41M5/42 , B41M5/46 , G11B15/18
CPC分类号： B41M5/465 , B41M5/305 , B41M5/34
摘要： A reversible multicolor thermal recording medium which is free from fogging and has sharp contrast even after recording and erasing are performed repeatedly, and a recording method using the same are provided. A reversible multicolor recording medium is provided, which includes recording layers each containing a plurality of reversible thermal coloring compositions having different coloring tones, formed to be separated from and stacked on a surface direction of a supporting substrate; and the plurality of reversible thermal coloring compositions containing light-to-heat transforming materials which absorb infrared rays having different wavelength ranges to generate heat, respectively; wherein an absorption peak wavelength of the light-to-heat transforming material contained in the recording layers becomes the longest wavelength at the layer formed nearest the supporting substrate, and becomes a shorter wavelength as the layer is closer to the surface layer in the stacked order.
摘要翻译：重复执行即使在记录和擦除之后也不起雾并具有尖锐对比度的可逆多色热记录介质，并且提供使用该多色热记录介质的记录方法。提供了一种可逆的多色记录介质，其包括各自包含多个具有不同着色色调的可逆热着色组合物的记录层，其形成为在支撑基板的表面方向上分离并层叠; 以及含有吸收具有不同波长范围的红外线以分别发热的光 - 热转换材料的多个可逆热着色组合物; 其中包含在记录层中的光 - 热转换材料的吸收峰波长在最靠近支撑衬底的层处成为最长波长，并且随着层以堆叠顺序更靠近表面层而变成较短的波长。

10. 发明授权

US06549608B1 Semiconductor manufacturing apparatus and semiconductor device manufacturing method 失效
标题翻译：半导体制造装置及半导体装置的制造方法
公开(公告)号：US06549608B1
公开(公告)日：2003-04-15
申请号：US09538737
申请日：2000-03-30
申请人： Takayoshi Mamine , Nobuyuki Matsuzawa , Noriyuki Kishii
发明人： Takayoshi Mamine , Nobuyuki Matsuzawa , Noriyuki Kishii
IPC分类号： G21K500
CPC分类号： G03F7/70008
摘要： To provide a semiconductor manufacturing apparatus and a semiconductor device manufacturing method able to form a sufficiently precise pattern by ablation. A semiconductor manufacturing apparatus comprising a light source emitting light of a first wavelength on the surface of a wafer and a mask through which at least a part of the light of the first wavelength passes and removing a material of the part of the wafer exposed by the light of the first wavelength by vaporization, wherein the light source comprises an electron beam generating means for generating an electron beam and a light emitting means for emitting light of a second wavelength longer than the first wavelength and wherein the light of the first wavelength is inverse Compton scattered light obtained by collision of electrons in the electron beam with photons in the light of the second wavelength causing the energy of the electrons to be given to the photons and a semiconductor device manufacturing method using the apparatus.
摘要翻译： 1.一种半导体制造装置，其特征在于，具有通过烧蚀形成足够精细的图案的半导体制造装置和半导体装置的制造方法。1。一种半导体制造装置，其特征在于，包括在晶片表面上发射第一波长的光的光源和至少一个第一波长的光的一部分通过蒸发而通过并除去由第一波长的光曝光的晶片的部分的材料，其中光源包括用于产生电子束的电子束产生装置和发光装置用于发射比第一波长长的第二波长的光，其中第一波长的光是通过电子束中的电子与第二波长的光子碰撞而获得的康普顿散射光，导致电子的能量给予光子和使用该设备的半导体器件制造方法 s。

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