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1. 发明申请

US20090311753A1 Method for amplifying genomic DNA 失效
标题翻译：扩增基因组DNA的方法
公开(公告)号：US20090311753A1
公开(公告)日：2009-12-17
申请号：US12214044
申请日：2008-06-16
申请人： Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
发明人： Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
IPC分类号： C12P19/34
CPC分类号： C12Q1/6846 , C12Q2565/518 , C12Q2531/119 , C12Q2527/119 , C12Q2527/101
摘要： A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
摘要翻译：提供了扩增基因组DNA的方法。该方法包括以下步骤：（1）在9至12的pH和45至80℃的温度下培养含细胞的琼脂糖溶液以产生基因组DNA分散的琼脂糖溶液，其中0.002-1拷贝/ 5 单链基因组DNA的微升分散; （2）固定基因组DNA分散的琼脂糖溶液以产生基因组DNA分散的琼脂糖凝胶并中和凝胶的pH; 和（3）将具有链置换活性的DNA聚合酶，引物和dNTP加入到基因组DNA分散的琼脂糖凝胶中，并在0至60℃的温度下孵育该凝胶以扩增基因组DNA。

2. 发明授权

US07678543B2 Method for amplifying genomic DNA 失效
标题翻译：扩增基因组DNA的方法
公开(公告)号：US07678543B2
公开(公告)日：2010-03-16
申请号：US12214044
申请日：2008-06-16
申请人： Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
发明人： Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
IPC分类号： C12Q1/68 , C12P19/34 , C07H2/04
CPC分类号： C12Q1/6846 , C12Q2565/518 , C12Q2531/119 , C12Q2527/119 , C12Q2527/101
摘要： A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
摘要翻译：提供了扩增基因组DNA的方法。该方法包括以下步骤：（1）在9至12的pH和45至80℃的温度下培养含细胞的琼脂糖溶液以产生基因组DNA分散的琼脂糖溶液，其中0.002-1拷贝/ 5 单链基因组DNA的微升分散; （2）固定基因组DNA分散的琼脂糖溶液以产生基因组DNA分散的琼脂糖凝胶并中和凝胶的pH; 和（3）将具有链置换活性的DNA聚合酶，引物和dNTP加入到基因组DNA分散的琼脂糖凝胶中，并在0至60℃的温度下孵育该凝胶以扩增基因组DNA。

3. 发明申请

US20090317813A1 METHOD OF PREDICTING METASTASIS OF ORAL CAVITY CANCER INTO CERVICAL LYMPH NODE AND DIAGNOSIS KIT TO BE USED IN THE PREDICTION 失效
标题翻译：将口腔癌预后的方法预测用于预测的宫颈淋巴结和诊断试剂盒
公开(公告)号：US20090317813A1
公开(公告)日：2009-12-24
申请号：US12310331
申请日：2007-03-30
申请人： Takashi Imai , Mayumi Iwakawa , Hitoshi Shibuya , Masahiko Miura , Ryoichi Yoshimura , Hiroshi Watanabe
发明人： Takashi Imai , Mayumi Iwakawa , Hitoshi Shibuya , Masahiko Miura , Ryoichi Yoshimura , Hiroshi Watanabe
IPC分类号： C12Q1/68
CPC分类号： G01N33/57407 , C12Q1/6886 , C12Q2600/118 , G01N2333/705 , G01N2333/71
摘要： A method for objectively predicting possibility of metastasis to a cervical lymph node in an early stage for an individual case diagnosed as an oral cavity cancer, and a diagnosis kit to be used in the prediction are provided. The method includes a step of assaying expression amounts of metastasis prediction genes in which the expression amounts are changed between a metastasis group and a non-metastasis group, with respect to a sample collected from a primary legion of the oral cavity cancer. Further, the method includes a step of predicting the possibility of the metastasis by comparing the expression amounts of the metastasis gene group with the expression amounts of the metastasis prediction genes in a metastasis group and/or a non-metastasis group. Herein, the metastasis prediction gene group includes two genes MSR1 (NM_138716.1) and RET (M31213.1).
摘要翻译：提供了用于客观预测诊断为口腔癌的个别病例的早期颈部淋巴结转移可能性的方法，以及用于预测的诊断试剂盒。该方法包括相对于从口腔癌的主要团队收集的样品，测定转移组和非转移组之间的表达量发生变化的转移预测基因的表达量的步骤。此外，该方法包括通过将转移基因组的表达量与转移组和/或非转移组中的转移预测基因的表达量进行比较来预测转移的可能性的步骤。这里，转移预测基因组包括两个基因MSR1（NM_138716.1）和RET（M31213.1）。

4. 发明申请

US20100130376A1 DNA CHIP FOR PREDICTION OF OCCURRENCE OF LATE ADVERSE REACTION IN URINARY ORGAN AFTER RADIOTHERAPY, AND METHOD FOR PREDICTION OF OCCURRENCE OF LATE ADVERSE REACTION IN URINARY ORGAN AFTER RADIO THERAPY USING THE SAME 审中-公开
标题翻译：用于预测放射治疗后尿道反应性不良反应的DNA芯片，以及使用其进行无线电治疗后预防尿毒症反应最近的不良反应的方法
公开(公告)号：US20100130376A1
公开(公告)日：2010-05-27
申请号：US12452500
申请日：2008-07-07
申请人： Takashi Imai , Mayumi Iwakawa , Eisei Oda
发明人： Takashi Imai , Mayumi Iwakawa , Eisei Oda
IPC分类号： C40B30/04 , C40B40/08
CPC分类号： C12Q1/6883 , C12Q1/6837 , C12Q2600/106 , C12Q2600/142 , C12Q2600/156
摘要： A DNA chip and a prediction method for predicting the occurrence of a late adverse reaction in a urinary organ after C-ion RT are provided. The DNA chip comprises a supporting means for supporting a DNA probe thereon, and a plurality of genetic markers supported on the supporting means. The prediction method comprises a first step of hybridizing a genetic marker with a labeled DNA prepared from a subject to be examined, a second step of identifying bases of both alleles of the labeled DNA hybridized with the genetic marker, and a third step of determining a genotype of the labeled DNA as a risk genotype if the combination of the identified bases corresponds to the specified combination, and predicting that the subject is predisposed to develop a late adverse reaction in a urinary organ after radiotherapy when the number of the risk genotypes is three or more and the subject is not predisposed to develop a late adverse reaction in a urinary organ after radiotherapy when the number of the risk genotypes is two or less. The method enables to predict whether or not a subject is affected with a late adverse reaction in a urinary organ after radiotherapy.
摘要翻译：提供DNA芯片和用于预测C-ion RT后的尿器官晚期不良反应发生的预测方法。 DNA芯片包括用于在其上支撑DNA探针的支撑装置和支撑在支撑装置上的多个遗传标记。该预测方法包括：将遗传标记与由待检测对象制备的标记DNA杂交的第一步骤，鉴定与遗传标记杂交的标记DNA的两个等位基因的碱基的第二步骤，以及第三步骤，如果所鉴定的碱基的组合对应于指定的组合，则标记的DNA的基因型为风险基因型，并且当风险基因型的数量为3时，预测该受试者易于在放射治疗后在尿器官中发展晚期不良反应或以上，并且当风险基因型的数目为2或更小时，该受试者在放射治疗后不容易发生尿脏器官晚期不良反应。该方法能够预测放射治疗后尿液器官的晚期不良反应是否影响受试者。

5. 发明申请

US20070264648A1 Dna Oligomer, Genetic Marker and Dna Oligomer Set for Prediction of Onset of Side-Effect from Radiation Therapy, and Method for Predicting Onset of Side-Effect 审中-公开
标题翻译： Dna寡聚体，遗传标记和Dna寡聚体预测放射治疗副作用的预测及预测副作用发生的方法
公开(公告)号：US20070264648A1
公开(公告)日：2007-11-15
申请号：US11660658
申请日：2005-09-22
申请人： Takashi Imai , Yoshinobu Harada , Mayumi Iwakawa
发明人： Takashi Imai , Yoshinobu Harada , Mayumi Iwakawa
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6881 , C12Q1/6886 , C12Q2600/106 , C12Q2600/156
摘要： There are provided a DNA oligomer, a genetic marker, and a DNA oligomer set (PCR primer set) and a DNA oligomer (extension primer) for predicting a possibility of onset of a side-effect from radiation therapy for cancer by determining whether a specific base in a DNA sequence is a risk allele or a non-risk allele, and a method for predicting onset of a side-effect from radiation therapy. The DNA oligomer for a prediction of onset of a side-effect from radiation therapy has a DNA sequence of at least 10-241 contiguous bases with a 121st base from a sequence of any one of SEQ ID NOs: 1-173 in the Sequence Listing.
摘要翻译：提供DNA寡聚体，遗传标记和DNA寡聚体（PCR引物组）和DNA寡聚物（延伸引物），用于通过确定是否具体的特异性DNA来预测癌症放射治疗副作用的可能性 DNA序列中的碱基是风险等位基因或非风险等位基因，以及预测放射治疗副作用发生的方法。用于预测放射治疗副作用发作的DNA寡聚体具有至少10-241个连续碱基的DNA序列，其中序列表中SEQ ID NO：1-173中任一个的序列具有121个碱基。

6. 发明授权

US08129122B2 Method of predicting metastasis of oral cavity cancer into cervical lymph node and diagnosis kit to be used in the prediction 失效
标题翻译：预测口腔癌转移到宫颈淋巴结的方法和预测中使用的诊断试剂盒
公开(公告)号：US08129122B2
公开(公告)日：2012-03-06
申请号：US12310331
申请日：2007-03-30
申请人： Takashi Imai , Mayumi Iwakawa , Hitoshi Shibuya , Masahiko Miura , Ryoichi Yoshimura , Hiroshi Watanabe
发明人： Takashi Imai , Mayumi Iwakawa , Hitoshi Shibuya , Masahiko Miura , Ryoichi Yoshimura , Hiroshi Watanabe
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/11 , C12N15/12
CPC分类号： G01N33/57407 , C12Q1/6886 , C12Q2600/118 , G01N2333/705 , G01N2333/71
摘要： A method for objectively predicting possibility of metastasis to a cervical lymph node in an early stage for an individual case diagnosed as an oral cavity cancer, and a diagnosis kit to be used in the prediction are provided. The method includes a step of assaying expression amounts of metastasis prediction genes in which the expression amounts are changed between a metastasis group and a non-metastasis group, with respect to a sample collected from a primary legion of the oral cavity cancer. Further, the method includes a step of predicting the possibility of the metastasis by comparing the expression amounts of the metastasis gene group with the expression amounts of the metastasis prediction genes in a metastasis group and/or a non-metastasis group. Herein, the metastasis prediction gene group includes two genes MSR1 (NM_138716.1) and RET (M31213.1).
摘要翻译：提供了用于客观预测诊断为口腔癌的个别病例的早期颈部淋巴结转移可能性的方法，以及用于预测的诊断试剂盒。该方法包括相对于从口腔癌的主要团队收集的样品，测定转移组和非转移组之间的表达量发生变化的转移预测基因的表达量的步骤。此外，该方法包括通过将转移基因组的表达量与转移组和/或非转移组中的转移预测基因的表达量进行比较来预测转移的可能性的步骤。这里，转移预测基因组包括两个基因MSR1（NM_138716.1）和RET（M31213.1）。

7. 发明申请

US20110143336A1 MARKER FOR ESTIMATING THE DIAGNOSIS OF CERVICAL ADENOCARCINOMA OR FOR ESTIMATING THE PROGNOSIS OF CERVICAL CANCER 审中-公开
标题翻译：评估宫颈癌诊断标记或评估癌症预后的标记
公开(公告)号：US20110143336A1
公开(公告)日：2011-06-16
申请号：US12996908
申请日：2008-10-10
申请人： Takashi Imai , Mayumi Iwakawa , Shingo Kato , Tatsuya Ohno
发明人： Takashi Imai , Mayumi Iwakawa , Shingo Kato , Tatsuya Ohno
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/53
CPC分类号： G01N33/57442 , G01N33/57411 , G01N2333/025 , G01N2800/52 , G01N2800/56
摘要： To provide a novel biomarker for estimating the diagnosis of cervical adenocarcinoma or for estimating the prognosis of cervical cancer. An antibody against Villin 1 is employed as a biomarker.
摘要翻译：提供一种用于估计宫颈腺癌诊断或估计子宫颈癌预后的新型生物标志物。使用抗Villin 1的抗体作为生物标志物。

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