专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20090208975A1 Device and methods for detecting a target cell 审中-公开
标题翻译：用于检测靶细胞的装置和方法
公开(公告)号：US20090208975A1
公开(公告)日：2009-08-20
申请号：US12314598
申请日：2008-12-12
申请人： Sybil D'Costa , Robert S. Matson , Alexander Burshteyn , Michael Simonian , Jinghai Wen
发明人： Sybil D'Costa , Robert S. Matson , Alexander Burshteyn , Michael Simonian , Jinghai Wen
IPC分类号： G01N33/567 , C12M1/34
CPC分类号： G01N33/558 , B01L3/0282 , B01L3/5023 , B01L3/502746 , B01L3/502761 , B01L2200/0668 , B01L2300/069 , B01L2300/0825 , B01L2300/0851 , B01L2300/161 , B01L2400/0406 , B01L2400/086 , G01N33/505 , G01N33/54386 , G01N2333/7051 , G01N2333/70514 , G01N2333/70596
摘要： The present invention relates to devices for detecting intact target cells in a sample comprising a detection zone comprising an immobilized specific binding reagent, capable of forming a complex with a target analyte on a target cell. Once labeled, detection of the label indicates the absence, presence and/or amount of the target cell in a sample. The embodiments further relate to kits comprising the devices, and methods of using the devices to screen for the presence, absence, and/or amount of a target cell in a sample.
摘要翻译：本发明涉及用于检测样品中完整靶细胞的装置，其包括检测区，该检测区包含固定的特异性结合试剂，能够与靶细胞上的靶分析物形成复合物。一旦标记，标签的检测表明样品中靶细胞的不存在，存在和/或量。实施例还涉及包括该装置的试剂盒，以及使用该装置筛选样品中目标细胞的存在，不存在和/或量的方法。

2. 发明授权

US06841379B2 Conductive microplate 有权
标题翻译：导电微孔板
公开(公告)号：US06841379B2
公开(公告)日：2005-01-11
申请号：US10146004
申请日：2002-05-15
申请人： Robert S. Matson
发明人： Robert S. Matson
IPC分类号： G01N33/53 , B01J19/00 , B01L3/00 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N33/566 , G01N37/00
CPC分类号： B01L3/50851 , B01J19/0046 , B01J2219/00317 , B01J2219/00585 , B01J2219/00596 , B01J2219/00639 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01L2300/069 , B01L2300/0829 , B01L2300/1827 , B01L2400/0421 , C40B40/06 , C40B60/14 , Y10S435/817
摘要： A conductive microplate device for the detection of target biomolecules in a sample is described. The microplate comprises an assembly of a porous substrate and a conductive layer, wherein the assembly is sealed into bottom of at least some wells of the microplate. The porous substrate has a top surface and a bottom surface. The top surface comprises a plurality of covalently attached probe biomolecules. The covalently attached probe biomolecules are reactive with the target biomolecules contained in the sample. The conductive layer, which is attached to the bottom surface of the porous substrate, is adapted to receive voltage. Microplates of the present invention can be easily adapted for use with robotic workstations. Accordingly, in one embodiment, the power supply is incorporated into a robotic arm tool for fast microplate processing.
摘要翻译：描述了用于检测样品中目标生物分子的导电微孔板装置。微孔板包括多孔基底和导电层的组件，其中将组件密封在微板的至少一些孔的底部。多孔基材具有顶表面和底表面。顶表面包括多个共价附着的探针生物分子。共价连接的探针生物分子与样品中包含的目标生物分子具有反应性。连接到多孔基底的底表面的导电层适于接收电压。本发明的微孔板可以容易地适用于机器人工作站。因此，在一个实施例中，电源被并入用于快速微孔板处理的机器人手臂工具中。

3. 发明授权

US5554501A Biopolymer synthesis using surface activated biaxially oriented polypropylene 失效
标题翻译：使用表面活性双轴取向聚丙烯的生物聚合物合成
公开(公告)号：US5554501A
公开(公告)日：1996-09-10
申请号：US145939
申请日：1993-10-29
申请人： Peter J. Coassin , Robert S. Matson , Jang B. Rampal
发明人： Peter J. Coassin , Robert S. Matson , Jang B. Rampal
IPC分类号： G01N33/53 , A61K38/00 , A61K39/44 , B01J19/00 , C07H21/00 , C07H21/04 , C07K1/04 , C07K7/06 , C07K7/08 , C08B37/00 , C12N15/09 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B40/12 , G01N33/543 , G01N33/545 , G01N33/566 , G01N37/00 , G01N35/48
CPC分类号： C07H21/00 , B01J19/0046 , C07K1/042 , C12Q1/6834 , C12Q1/6869 , G01N33/54353 , B01J2219/00497 , B01J2219/00527 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , C40B40/06 , C40B40/10 , C40B40/12 , Y10T436/143333
摘要： Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated biaxially oriented polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.
摘要翻译：本文公开了可用于生物聚合物合成的表面活化的有机聚合物。最优选地，胺化双轴取向聚丙烯用于合成寡核苷酸，并且这些装置最优选用于患者样品的遗传分析。

4. 发明授权

US6037124A Carboxylated polyvinylidene fluoride solid supports for the immobilization of biomolecules and methods of use thereof 失效
标题翻译：用于固定生物分子的羧化聚偏二氟乙烯固体载体及其使用方法
公开(公告)号：US6037124A
公开(公告)日：2000-03-14
申请号：US720307
申请日：1996-09-27
申请人： Robert S. Matson
发明人： Robert S. Matson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6834
摘要： The present invention relates to a method of immobilizing oligonucleotides and other biomolecules using a carboxylated polyvinylidene fluoride support membrane for the construction of geosensors and other array-based systems. The support membrane may be used in either covalent or non-covalent binding states.
摘要翻译：本发明涉及使用羧化聚偏二氟乙烯支撑膜固定寡核苷酸和其他生物分子的方法，用于构建地理传感器和其它基于阵列的系统。支持膜可以以共价或非共价结合态使用。

5. 发明授权

US5981185A Oligonucleotide repeat arrays 失效
标题翻译：寡核苷酸重复阵列
公开(公告)号：US5981185A
公开(公告)日：1999-11-09
申请号：US863639
申请日：1997-05-28
申请人： Robert S. Matson , Peter J. Coassin , Jang B. Rampal , Charles Thomas Caskey
发明人： Robert S. Matson , Peter J. Coassin , Jang B. Rampal , Charles Thomas Caskey
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6874
摘要： A solid support based hybridization assay is provided which allows for the systematic and reproducible analysis of repeat and tandem repeat oligonucleotide sequences of DNA and RNA by hybridization to a reverse dot blot array comprising strings of such repeats complementary to those found in particular nucleic acid targets (e.g., analyte PCR product). An addressable library (i.e., an indexed set) of complementary repeats is synthesized on a suitable support. Preferably, the support comprises a low fluorescent background support, thereby facilitating the use of non-radioisotopic modes of detection (such as fluorescence or chemiluminescence); particularly suitable in this regard is an aminated polypropylene support or similar material. Preferred arrays permit screening of DNA and RNA samples for complete sets of particular types of nucleotide repeat sequences (e.g., all nucleotide doublet or triplet repeats).
摘要翻译：提供了基于固体支持的杂交测定法，其通过与包含与特定核酸靶标中发现的重复序列互补的反向斑点印迹阵列杂交，从而对DNA和RNA的重复和串联重复寡核苷酸序列进行系统和可重复的分析（例如分析物PCR产物）。互补重复序列的可寻址库（即，索引集）在合适的支持上合成。优选地，载体包含低荧光背景载体，从而有利于使用非放射性同位素检测模式（如荧光或化学发光）; 在这方面特别合适的是胺化聚丙烯支撑体或类似材料。优选的阵列允许筛选DNA和RNA样品用于完整的特定类型的核苷酸重复序列（例如，所有核苷酸双重或三重重复）。

6. 发明授权

US07070740B1 Method and apparatus for processing biomolecule arrays 有权
标题翻译：用于处理生物分子阵列的方法和装置
公开(公告)号：US07070740B1
公开(公告)日：2006-07-04
申请号：US09675020
申请日：2000-09-28
申请人： Robert S. Matson , Daniel G. Morrow , Karen E. Lowe
发明人： Robert S. Matson , Daniel G. Morrow , Karen E. Lowe
IPC分类号： B01L9/00
CPC分类号： B01L3/5085 , B01J19/0046 , B01J2219/00315 , B01J2219/00484 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , B01J2219/00662 , B01J2219/00691 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： A microarray assay system using an array-of-arrays plate mounted on the top surface of a vacuum fixture, and an automatic handling system that handles the plate and fixture assembly. The A2 plate includes a tray formed of a flexible material and a rigid frame for mounting on the fixture. The fixture has a plurality of orifices opening at the top surface and connected to a vacuum source to create a negative pressure to hold the flexible plate against the top surface of the fixture. The automatic handling systems that can be used with the plate and fixture assembly include a microarray printing machine, liquid handling robot, hybridization and incubation devices, and microarray imaging devices.
摘要翻译：使用安装在真空夹具顶表面上的阵列阵列的微阵列测定系统以及处理板和夹具组件的自动处理系统。 A＆lt; 2＆gt;板包括由柔性材料形成的托盘和用于安装在固定装置上的刚性框架。夹具具有多个在顶表面处开口并连接到真空源的孔，以产生负压以将柔性板保持抵靠固定装置的顶表面。可与板和夹具组合件一起使用的自动处理系统包括微阵列印刷机，液体处理机器人，杂交和孵化装置以及微阵列成像装置。

7. 发明授权

US06861214B1 Immobilization of biopolymers to aminated substrates by direct adsorption 有权
公开(公告)号：US06861214B1
公开(公告)日：2005-03-01
申请号：US09694701
申请日：2000-10-23
申请人： Jang B. Rampal , Robert S. Matson
发明人： Jang B. Rampal , Robert S. Matson
IPC分类号： G01N33/53 , C07B61/00 , C07H21/00 , C12M1/00 , C12N15/09 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/543 , G01N33/545 , G01N37/00 , A61L9/00 , A62B7/08 , B01J19/08 , B32B27/04
CPC分类号： C07H21/00 , B01J2219/00274 , B01J2219/00315 , B01J2219/00367 , B01J2219/00378 , B01J2219/00387 , B01J2219/00497 , B01J2219/00515 , B01J2219/00518 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/0063 , B01J2219/00635 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C07B2200/11 , C40B40/06 , C40B40/10 , C40B60/14
摘要： An assay article for detection biopolymers contained in a sample is described. The assay article includes a substrate and a biopolymer directly adsorbed on the surface of the substrate. A plurality of biopolymers may be adsorbed on the surface of the substrate to form an array. Also disclosed is a method of making the assay article. In the preferred method, an aminated polypropylene substrate is used. A biopolymer is contacted with the aminated substrate under a condition sufficient for direct adsorption of the biopolymer on the surface of the substrate. A method of detecting a target biopolymer contained in a sample is also disclosed. In this method, a substrate is contacted with either a probe or target biopolymer under a condition sufficient for a direct adsorption of either the probe or target biopolymer on the substrate to form a probe assay article or a target assay article. Then, the probe assay article is contacted with the target biopolymer, or the target assay article is contacted with the probe biopolymer under a condition that allows the formation of a probe-target complex. Finally, the complex is detected and the presence of the complex is used as a measurement for the presence or the amount of the biopolymer target contained in the sample.

8. 发明授权

US06984485B2 Polymer-coated substrates for immobilization of biomolecules and cells 有权
公开(公告)号：US06984485B2
公开(公告)日：2006-01-10
申请号：US10128350
申请日：2002-04-23
申请人： Robert S. Matson
发明人： Robert S. Matson
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00351 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00635 , B01J2219/00659 , C40B60/14
摘要： Methods for preparing a substrate for the arraying and immobilizing of biomolecules or cells are described. The methods include providing a solid support and depositing by a chemical plasma-mediated polymerization process a polymeric coating on the surface. The polymeric coating comprises at least one pendant functional group capable of attaching a biomolecule or a cell. A material of the polymeric coating is preferably selected in such a way that its surface properties enhance binding between the pendant group and the biomolecule or cell. A substrate for the immobilization of biomolecules and cells is also provided. The substrate comprises a solid support made of a polymeric material. The solid support has at least one first pendant functional group suitable for attaching a first biomolecule or a first cell on its surface. The substrate further comprises a polymeric coating deposited on the surface of the substrate. The polymeric coating increases the attachment of the first biomolecule or the first cell to the first functional group.

9. 发明授权

US06929944B2 Analysis using a distributed sample 失效
标题翻译：使用分布式样本进行分析
公开(公告)号：US06929944B2
公开(公告)日：2005-08-16
申请号：US09945145
申请日：2001-08-31
申请人： Robert S. Matson
发明人： Robert S. Matson
IPC分类号： B01L3/00 , C12Q1/68 , C40B30/04 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G01N33/68 , C12M1/34 , A61K38/00 , C07H1/00 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C40B30/04 , B01J2219/00315 , B01J2219/00367 , B01J2219/00378 , B01J2219/00385 , B01J2219/00387 , B01J2219/005 , B01J2219/00509 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/00743 , B01L3/508 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G01N33/6845 , C12Q2565/518
摘要： The present invention is directed to the production of a sample microarray for use in detecting one or more target biopolymers in the sample. The sample microarray of this invention is formed by distributing equivalent amounts of a single sample at discrete, spatially defined locations on a substrate. Each site in the microarray, thus, has the same composition of target biopolymers. The microarray is then interrogated by one or more probes specific for one or more the target biopolymers.
摘要翻译：本发明涉及用于检测样品中一种或多种目标生物聚合物的样品微阵列的制备。本发明的样品微阵列通过在衬底上的离散的空间限定位置处分配等量的单个样品而形成。因此，微阵列中的每个位点具有相同的靶生物聚合物组成。然后通过一种或多种针对一种或多种目标生物聚合物特异性的探针询问微阵列。

10. 发明授权

US06268141B1 Immobilization of unmodified biopolymers to acyl fluoride activated substrates 失效
标题翻译：将未改性的生物聚合物固定在酰基氟化物活化的底物上
公开(公告)号：US06268141B1
公开(公告)日：2001-07-31
申请号：US09312095
申请日：1999-05-12
申请人： Robert S. Matson , Raymond C. Milton
发明人： Robert S. Matson , Raymond C. Milton
IPC分类号： C12Q168
CPC分类号： G01N33/54353 , C12Q1/6834 , Y10S530/81 , Y10S530/811 , Y10S530/815
摘要： A method of attaching unmodified biopolymers, particularly, unmodified polynucleotides, directly to a solid support is provided. The method includes the steps of (a) providing unmodified biopolymers; (b) providing a solid support having at least one surface comprising pendant acyl fluoride functionalities, and (c) contacting the unmodified biopolymers with the solid support under a condition sufficient for allowing the attachment of the biopolymers to the solid support. The unmodified biopolymers may be nucleic acids, polypeptides, proteins, carbohydrates, lipids and analogues thereof. The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides. The DNA may be single or double stranded. A device including a solid support and unmodified biopolymers attached to the solid support by reaction with the pendant acyl fluoride functionalities of the solid support is also provided. The methods and devices of the present invention may be used in performing hybridization assays and immunoassays.
摘要翻译：提供了将未改性的生物聚合物，特别是未修饰的多核苷酸直接连接到固体支持物上的方法。该方法包括以下步骤：（a）提供未改性的生物聚合物; （b）提供固体支持物，其具有至少一个包含酰基侧酰氟官能团的表面，和（c）在足以允许将生物聚合物连接到固体支持物的条件下使未改性的生物聚合物与固体支持物接触。未修饰的生物聚合物可以是核酸，多肽，蛋白质，碳水化合物，脂质及其类似物。未修饰的多核苷酸可以是DNA，RNA或合成的寡核苷酸。 DNA可以是单链或双链。还提供了包括固体支持物和通过与固体支持物的酰基侧酰氟官能团反应连接到固体支持物上的未改性生物聚合物的装置。本发明的方法和装置可用于进行杂交测定和免疫测定。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式