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1. 发明授权

US5910433A Keto group-introducing enzyme, DNA coding therefor and method for producing ketocarotenoids 失效
标题翻译：酮基引入酶，其编码的DNA和生产酮类胡萝卜素的方法
公开(公告)号：US5910433A
公开(公告)日：1999-06-08
申请号：US632434
申请日：1996-04-23
申请人： Susumu Kajiwara , Norihiko Misawa , Keiji Kondo
发明人： Susumu Kajiwara , Norihiko Misawa , Keiji Kondo
IPC分类号： C12N1/21 , C12N9/02 , C12N15/53 , C12P7/26 , C12P23/00 , C07H21/04 , C12N1/12
CPC分类号： C12N9/0004 , C12P23/00 , C12P7/26 , Y10S435/946
摘要： DNA sequences are described that encode genes for synthesizing ketocarotenoids such as astaxanthin. The DNA sequences, microorganisms containing them and encoded polypeptides are described. Also described are methods to obtain related sequences and to make host cells that contain such sequences. These genes and methods are useful to impart red coloration during culture of fish and crustaceans, as coloring in food, and as antioxidants.
摘要翻译： PCT No.PCT / JP95 / 01640 Sec。 371日期：1996年4月23日 102（e）日期1996年4月23日PCT提交1995年8月18日PCT公布。出版物WO96 / 06172 日期1996年2月29日描述了编码用于合成酮类胡萝卜素如虾青素的基因的DNA序列。描述了DNA序列，含有它们的微生物和编码的多肽。还描述了获得相关序列并制备含有这种序列的宿主细胞的方法。这些基因和方法可用于在鱼和甲壳类动物培养期间赋予红色，作为食物中的着色以及作为抗氧化剂。

2. 发明授权

US06821749B1 Methods of producing carotenoids using DNA molecules encoding isopentenyl pyrophosphate isomerase 失效
标题翻译：使用编码异戊烯基焦磷酸异构酶的DNA分子生产类胡萝卜素的方法
公开(公告)号：US06821749B1
公开(公告)日：2004-11-23
申请号：US08737319
申请日：1996-11-12
申请人： Susumu Kajiwara , Norihiko Misawa , Keiji Kondo
发明人： Susumu Kajiwara , Norihiko Misawa , Keiji Kondo
IPC分类号： C12P2300
CPC分类号： C12P23/00 , C12N9/90
摘要： The production of carotenoid is accomplished using a DNA molecule that encodes a polypeptide as obtained from Haematococcus pluvialis, Phaffia rhodozyma, or Saccharomyces cerevisiae, having isopentonyl pyrophosphate (IPP) isomerase activity, or DNA molecule having a nucleotide sequence that hybridizes thereto. In particular, one can introduce such a DNA molecule into a carotenoid-producing microorganism, culture the microorganism thus transformed, and then obtain carotenoids in the culture broth and cells.
摘要翻译：类胡萝卜素的生产使用编码由具有异戊基焦磷酸（IPP）异构酶活性的红潮球菌，红酵母或酿酒酵母获得的多肽的DNA分子或具有与其杂交的核苷酸序列的DNA分子来完成。特别地，可以将这样的DNA分子引入到产生类胡萝卜素的微生物中，培养如此转化的微生物，然后在培养液和细胞中获得类胡萝卜素。

3. 发明授权

US5849524A Transformation systems for the yeast candida utilis and the expression of heterologous genes therewith 失效
标题翻译：用于产酵母酵母的转化体系及其异源基因的表达
公开(公告)号：US5849524A
公开(公告)日：1998-12-15
申请号：US557128
申请日：1996-05-24
申请人： Keiji Kondo , Susumu Kajiwara , Norihiko Misawa
发明人： Keiji Kondo , Susumu Kajiwara , Norihiko Misawa
IPC分类号： C12N15/09 , C07H21/04 , C07K14/40 , C12N1/19 , C12N9/14 , C12N9/88 , C12N15/31 , C12N15/81 , C12P21/02 , C12R1/72
CPC分类号： C12N9/14 , C07K14/40 , C12N15/815 , C12N9/88
摘要： An reproducible transformation system of a yeast of Candida utilis, a process for expressing a heterologous gene in the transformation system, a vector which can be used in the transformation system and the expression method, and a novel DNA group are disclosed. In particular, the process for expressing a heterologous gene in Candida utilis comprises transforming Candida utilis with a vector comprising a drug-resistance marker, a sequence homologous to the chromosomal DNA of the Candida utilis yeast, and the heterologous gene, culturing the transformant, and isolating the expression product of the heterologous gene.
摘要翻译： PCT No.PCT / JP95 / 01005 Sec。 371日期：1996年5月24日 102（e）日期1996年5月24日PCT提交1995年5月25日PCT公布。出版物WO95 / 32289 日期：1995年11月30日公开了产朊假丝酵母酵母的可再现转化体系，转化体系中表达异源基因的方法，可用于转化体系和表达方法的载体和新的DNA基团。特别地，用于在产朊假丝酵母中表达异源基因的方法包括用包含药物抗性标记的载体转化假丝酵母，与产朊假丝酵母的染色体DNA同源的序列和培养转化体的异源基因，以及分离异源基因的表达产物。

4. 发明授权

US6150130A DNA strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls 有权
标题翻译：可用于合成叶黄素的DNA链和产生叶黄素的方法
公开(公告)号：US6150130A
公开(公告)日：2000-11-21
申请号：US335919
申请日：1999-06-18
申请人： Norihiko Misawa , Keiji Kondo , Susumu Kajiwara , Akihiro Yokoyama
发明人： Norihiko Misawa , Keiji Kondo , Susumu Kajiwara , Akihiro Yokoyama
IPC分类号： C12N9/02 , C12N15/53 , C12P23/00 , C12P7/26
CPC分类号： C12N9/0093 , C12N9/0004 , C12P23/00
摘要： Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring.It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
摘要翻译：公开了与合成含有酮基的叶黄素如虾青素等相关的以下DNA链，以及通过基因工程与叶黄素的生产有关的技术：具有编码具有酶的多肽的核苷酸序列的DNA链将β-丁酮环的4-位上的亚甲基转化为酮基的活性。具有编码具有将3-羟基-β-雄酮环的4-位的亚甲基转换为酮基的酶活性的多肽的核苷酸序列的DNA链。一种DNA链，其具有编码具有用于向4-酮-β-雄酮环的3-碳中添加羟基的酶活性的多肽的核苷酸序列。通过将DNA链引入适当的微生物如大肠杆菌等可以产生各种叶黄素如角黄素，虾青素等。

5. 发明授权

US5972690A DNA strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls 失效
标题翻译：可用于合成叶黄素的DNA链和产生叶黄素的方法
公开(公告)号：US5972690A
公开(公告)日：1999-10-26
申请号：US6491
申请日：1998-01-13
申请人： Norihiko Misawa , Keiji Kondo , Susumu Kajiwara , Akihiro Yokoyama
发明人： Norihiko Misawa , Keiji Kondo , Susumu Kajiwara , Akihiro Yokoyama
IPC分类号： C12N9/02 , C12N15/53 , C12P23/00 , C12N1/20 , C07H21/04
CPC分类号： C12N9/0093 , C12N9/0004 , C12P23/00
摘要： Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:A DNA strand having a nucleotide sequence which encodes a polypeptides having and enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring.It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
摘要翻译：公开了与合成含有酮基的叶黄素如虾青素等有关的以下DNA链，以及通过遗传工程生产叶黄素的技术：具有编码具有和酶的多肽的核苷酸序列的DNA链将β-丁酮环的4-位上的亚甲基转化为酮基的活性。具有编码具有将3-羟基-β-雄酮环的4-位的亚甲基转换为酮基的酶活性的多肽的核苷酸序列的DNA链。一种DNA链，其具有编码具有用于向4-酮-β-雄酮环的3-碳中添加羟基的酶活性的多肽的核苷酸序列。通过将DNA链引入适当的微生物如大肠杆菌等可以产生各种叶黄素如角黄素，虾青素等。

6. 发明授权

US5811273A DNA strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls 失效
标题翻译：可用于合成叶黄素的DNA链和产生叶黄素的方法
公开(公告)号：US5811273A
公开(公告)日：1998-09-22
申请号：US663310
申请日：1996-09-23
申请人： Norihiko Misawa , Keiji Kondo , Susumu Kajiwara , Akihiro Yokoyama
发明人： Norihiko Misawa , Keiji Kondo , Susumu Kajiwara , Akihiro Yokoyama
IPC分类号： C12N9/02 , C12N15/53 , C12P23/00 , C12P7/26
CPC分类号： C12N9/0093 , C12N9/0004 , C12P23/00
摘要： Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering: A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of .beta.-ionone ring into a keto group. A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group. A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring. It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
摘要翻译： PCT No.PCT / JP94 / 02220 Sec。 371日期1996年9月23日 102（e）日期1996年9月23日PCT 1994年12月26日PCT PCT。公开号WO95 / 18220 日期1995年7月6日公开了以下关于合成含有酮基的叶黄素如虾青素等的DNA链，以及通过遗传工程生产叶黄素的技术：具有编码的核苷酸序列的DNA链具有用于将β-丁酮环4位的亚甲基转化为酮基的酶活性的多肽。具有编码具有将3-羟基-β-雄酮环的4-位的亚甲基转换为酮基的酶活性的多肽的核苷酸序列的DNA链。一种DNA链，其具有编码具有用于向4-酮-β-雄酮环的3-碳中添加羟基的酶活性的多肽的核苷酸序列。通过将DNA链引入适当的微生物如大肠杆菌等可以产生各种叶黄素如角黄素，虾青素等。

7. 发明授权

US06387688B1 DNA fragments having basidiomycete-derived promoter activity and expression of foreign genes under control of the promoter activity 失效
标题翻译：具有担子菌纲衍生的启动子活性的DNA片段和外源基因在启动子活性控制下的表达
公开(公告)号：US06387688B1
公开(公告)日：2002-05-14
申请号：US09503505
申请日：2000-02-14
申请人： Kazuo Shishido , Susumu Kajiwara , Akira Tsukamoto
发明人： Kazuo Shishido , Susumu Kajiwara , Akira Tsukamoto
IPC分类号： C12N114
CPC分类号： C12N9/0061 , C07K14/37 , C12N9/0065
摘要： This invention relates to a Coriolus hirsutus host cell transformed with a vector containing a basidiomycete-derived promoter region selected from the group consisting of basidiomycete-derived ras and priA gene promoter regions. This invention is particularly characterized in that the basidiomycete-derived promoter region is a Lentinus edodes-derived ras or priA gene promoter region or a Coriolus hirsutus-derived ras gene promoter region. This host cell can be utilized for the high productivity of lignin degrading enzymes.
摘要翻译：本发明涉及一种用含有担子菌纲衍生的启动子区域的载体转化的陆地毛球菌宿主细胞，该区域选自担纲菌来源的ras和priA基因启动子区域。本发明特别的特征在于担纲菌来源的启动子区域是香菇来源的ras或priA基因启动子区域或来自陆地弯曲菌的ras基因启动子区域。该宿主细胞可用于木质素降解酶的高生产率。

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