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1. 发明申请

US20110028375A1 Fungal serine protease and use thereof 有权
标题翻译：真菌丝氨酸蛋白酶及其用途
公开(公告)号：US20110028375A1
公开(公告)日：2011-02-03
申请号：US12799638
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo , George Szakacs
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo , George Szakacs
IPC分类号： C11D3/386 , A23J3/34 , C12N9/58 , C12N9/62 , C12N1/00 , C12N1/15 , C12N1/02 , C12N15/63 , C07H21/00 , C11D3/60
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得，更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO：12的质粒pALK2530和保藏的全长基因SEQ ID NO：13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中，以及真菌宿主如Trichoderm。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

2. 发明授权

US08609390B2 Fungal serine protease and use thereof 有权
标题翻译：真菌丝氨酸蛋白酶及其用途
公开(公告)号：US08609390B2
公开(公告)日：2013-12-17
申请号：US12799638
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo , George Szakacs
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo , George Szakacs
IPC分类号： C12N9/68
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得，更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO：12的质粒pALK2530和保藏的全长基因SEQ ID NO：13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中，以及真菌宿主如Trichoderm。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

3. 发明授权

US08603795B2 Fungal protease and use thereof 有权
标题翻译：真菌蛋白酶及其用途
公开(公告)号：US08603795B2
公开(公告)日：2013-12-10
申请号：US12799639
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
IPC分类号： C12N9/64
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质物质修饰，降解或去除的应用。

4. 发明申请

US20110003729A1 Novel fungal protease and use thereof 有权
标题翻译：新型真菌蛋白酶及其用途
公开(公告)号：US20110003729A1
公开(公告)日：2011-01-06
申请号：US12799639
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
IPC分类号： C11D7/42 , C12N9/58 , C12N9/62 , C07H21/00 , C12N15/57 , C12P21/02 , A61K36/06
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质物质修饰，降解或去除的应用。

5. 发明申请

US20130065810A2 Novel Fungal Protease and Use Thereof 有权
标题翻译：新型真菌蛋白酶及其用途
公开(公告)号：US20130065810A2
公开(公告)日：2013-03-14
申请号：US12799639
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
IPC分类号： C11D7/42 , C12N9/62 , A61K36/06 , C12N15/57 , C12P21/02 , C12N9/58 , C07H21/00
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质物质修饰，降解或去除的应用。

6. 发明申请

US20110008870A1 Fungal protease and use thereof 有权
标题翻译：真菌蛋白酶及其用途
公开(公告)号：US20110008870A1
公开(公告)日：2011-01-13
申请号：US12803456
申请日：2010-06-28
申请人： Susanna Makinen , Pentti Ojapalo , Leena Valtakari , Kari Juntunen , Marja Paloheimo
发明人： Susanna Makinen , Pentti Ojapalo , Leena Valtakari , Kari Juntunen , Marja Paloheimo
IPC分类号： C12N9/58 , C12N1/15 , C12N15/63 , C07H21/04
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme useful in modification, degradation or removal of proteinaceous material, which enzyme comprises an amino acid sequence of the mature Tr Prb1 enzyme having an amino acid sequence of SEQ ID NO: 10 or a variant thereof having similar activity. The serine protease is obtainable from Trichoderma. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2650 comprising the nucleotide sequence SEQ ID NO: 10 of the full length enzyme deposited in E. coli RF8052 under accession number DSM 22635. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material at low or moderate temperature ranges.
摘要翻译：本发明涉及可用于蛋白质物质的修饰，降解或去除的真菌丝氨酸蛋白酶，该酶包含具有SEQ ID NO：10的氨基酸序列或其变体的成熟Tr Prb1酶的氨基酸序列具有相似的活动。丝氨酸蛋白酶可从木霉获得。还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22635在大肠杆菌RF8052中沉积的全长酶的核苷酸序列SEQ ID NO：10的质粒pALK2650。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于涉及在低或中等温度范围内修饰，降解或去除蛋白质物质的任何应用。

7. 发明申请

US20120252064A1 Protease Enzyme and Uses Thereof 有权
标题翻译：蛋白酶及其用途
公开(公告)号：US20120252064A1
公开(公告)日：2012-10-04
申请号：US13433984
申请日：2012-03-29
申请人： Leena Valtakari , Kari Juntunen , Marja Paloheimo , Pentti Ojapalo
发明人： Leena Valtakari , Kari Juntunen , Marja Paloheimo , Pentti Ojapalo
IPC分类号： C12N9/58 , C12N1/15 , D06M16/00 , C12S11/00 , A23J3/34 , C11D3/386 , C11D17/00 , C12P21/06 , C12N15/57 , C12N15/80 , C12N9/96
CPC分类号： C11D3/386 , C11D3/2065 , C11D11/0017 , C11D11/0023 , C12N9/58 , C12Y304/21
摘要： The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其中所述酶具有丝氨酸蛋白酶活性，并且包含如SEQ ID NO：18所定义的Malbranchea ALKO4122成熟蛋白酶的氨基酸序列或与SEQ ID NO：18所定义的氨基酸序列具有至少66％同一性的氨基酸序列 SEQ ID NO：18的氨基酸序列。还公开了分离的核酸分子，其包含编码真丝丝氨酸蛋白酶的多核苷酸序列，编码所述蛋白酶的核酸序列，宿主细胞和产生具有丝氨酸蛋白酶活性的多肽的方法。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，羊毛，毛发，皮革或丝绸，用于处理食品或饲料，或用于涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

8. 发明授权

US09404164B2 Protease enzyme and uses thereof 有权
标题翻译：蛋白酶及其用途
公开(公告)号：US09404164B2
公开(公告)日：2016-08-02
申请号：US13433984
申请日：2012-03-29
申请人： Leena Valtakari , Kari Juntunen , Marja Paloheimo , Pentti Ojapalo
发明人： Leena Valtakari , Kari Juntunen , Marja Paloheimo , Pentti Ojapalo
IPC分类号： C12N9/58 , C11D3/386
CPC分类号： C11D3/386 , C11D3/2065 , C11D11/0017 , C11D11/0023 , C12N9/58 , C12Y304/21
摘要： The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其中所述酶具有丝氨酸蛋白酶活性，并且包含如SEQ ID NO：18所定义的Malbranchea ALKO4122成熟蛋白酶的氨基酸序列或与SEQ ID NO：18所定义的氨基酸序列具有至少66％同一性的氨基酸序列 SEQ ID NO：18的氨基酸序列。还公开了分离的核酸分子，其包含编码真丝丝氨酸蛋白酶的多核苷酸序列，编码所述蛋白酶的核酸序列，宿主细胞和产生具有丝氨酸蛋白酶活性的多肽的方法。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，羊毛，毛发，皮革或丝绸，用于处理食品或饲料，或用于涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

9. 发明授权

US08362222B2 Fungal protease and use thereof 有权
标题翻译：真菌蛋白酶及其用途
公开(公告)号：US08362222B2
公开(公告)日：2013-01-29
申请号：US12803456
申请日：2010-06-28
申请人： Leena Valtakari , Kari Juntunen , Susanna Mäkinen , Pentti Ojapalo , Marja Paloheimo
发明人： Leena Valtakari , Kari Juntunen , Susanna Mäkinen , Pentti Ojapalo , Marja Paloheimo
IPC分类号： C07H21/04
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme useful in modification, degradation or removal of proteinaceous material, which enzyme comprises an amino acid sequence of the mature Tr Prb1 enzyme having an amino acid sequence of SEQ ID NO: 10 or a variant thereof having similar activity. The serine protease is obtainable from Trichoderma. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2650 comprising the nucleotide sequence SEQ ID NO: 10 of the full length enzyme deposited in E. coli RF8052 under accession number DSM 22635. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material at low or moderate temperature ranges.
摘要翻译：本发明涉及可用于蛋白质物质的修饰，降解或去除的真菌丝氨酸蛋白酶，该酶包含具有SEQ ID NO：10的氨基酸序列或其变体的成熟Tr Prb1酶的氨基酸序列具有相似的活动。丝氨酸蛋白酶可从木霉获得。还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22635在大肠杆菌RF8052中沉积的全长酶的核苷酸序列SEQ ID NO：10的质粒pALK2650。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于涉及在低或中等温度范围内修饰，降解或去除蛋白质物质的任何应用。

10. 发明申请

US20120107905A1 Variants of Fungal Serine Protease 有权
标题翻译：真菌丝氨酸蛋白酶的变体
公开(公告)号：US20120107905A1
公开(公告)日：2012-05-03
申请号：US13283891
申请日：2011-10-28
申请人： Kari Juntunen , Leena Valtakari , Nina Hakulinen , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Nina Hakulinen , Marja Paloheimo
IPC分类号： C12N9/58 , C11D3/60 , C12N1/15 , C12N15/57 , C12N15/63
CPC分类号： C12N9/58 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants. The serine protease variants are applicable in laundry and dish-washing detergent compositions, in treating fibers, in treating wool, in treating hair, in treating leather, in treating food or feed, or in any application involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶变体，其包含母本镰刀菌属Fe_RF6318丝氨酸蛋白酶的208位的缬氨酸的氨基酸取代，其中取代的位置对应于SEQ ID NO：1中定义的成熟Fe_RF6318酶的氨基酸序列编号：2。与母体Fe_RF6318酶相比，变体具有改善的热稳定性和/或洗涤剂稳定性。优选地，取代是V208I，更优选地，变体包含进一步增加稳定性的另外的氨基酸变化。还公开了编码所述蛋白酶变体的核酸序列以及用于产生变体的重组载体和宿主细胞。丝氨酸蛋白酶变体可用于洗衣和洗碟洗涤剂组合物，处理纤维，处理羊毛，处理毛发，处理皮革，处理食品或饲料，或涉及任何涉及蛋白质物质的改性，降解或去除的应用。

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