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1. 发明申请

US20050003392A1 Methods of small sample amplification 审中-公开
标题翻译：小样本扩增方法
公开(公告)号：US20050003392A1
公开(公告)日：2005-01-06
申请号：US10805559
申请日：2004-03-19
申请人： Susana Salceda , Kai Wu , Natalia Briones , Qing Bai , Yanxiang Cao
发明人： Susana Salceda , Kai Wu , Natalia Briones , Qing Bai , Yanxiang Cao
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C12Q1/68
摘要： The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction and the product of this reaction is used as template for subsequent rounds of amplification. Polyadenylated control transcripts are added to the nucleic acid sample prior to the first step of amplification to monitor the efficiency of the amplification and labeling reactions.
摘要翻译：本发明涉及核酸，优选mRNA的扩增。将引物和启动子加入待扩增的靶序列中，然后在体外转录反应中扩增靶，并将该反应的产物用作随后的扩增循环。将多腺苷酸化的对照转录物在扩增的第一步骤之前加入到核酸样品中，以监测扩增和标记反应的效率。

2. 发明申请

US20070218478A1 Methods for fragmentation and analysis of nucleic acid 审中-公开
标题翻译：核酸分离和分析方法
公开(公告)号：US20070218478A1
公开(公告)日：2007-09-20
申请号：US11612454
申请日：2006-12-18
申请人： Qing Bai , Susana Salceda , Kai Wu , Thong Nguyen , Charles Miyada
发明人： Qing Bai , Susana Salceda , Kai Wu , Thong Nguyen , Charles Miyada
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q2521/531 , C12Q2521/301 , C12Q2521/131
摘要： Methods for fragmenting and labeling DNA in a single reaction volume and incubation step using a uracil DNA glycosylase, an apurinic/apyrimidinic endonuclease, and a terminal transferase are disclosed. In a preferred embodiment the UDG, AP and TdT activities are first mixed together to form an enzyme mixture and then the enzyme mixture is mixed with the uracil containing DNA. The fragmentation and labeling reactions thus take place simultaneously as part of the same reaction. The methods may be used in a variety of applications where fragmenting and end-labeling single or double stranded DNA is desired.
摘要翻译：公开了在单个反应体积中分离和标记DNA的方法以及使用尿嘧啶DNA糖基化酶，无嘌呤/脱嘧啶核苷酸内切酶和末端转移酶的温育步骤。在优选的实施方案中，首先将UDG，AP和TdT活性混合在一起以形成酶混合物，然后将该酶混合物与含有尿嘧啶的DNA混合。因此，碎裂和标记反应同时作为相同反应的一部分进行。该方法可用于需要片段化和末端标记单链或双链DNA的多种应用。

3. 发明申请

US20070020654A1 Methods and kits for preparing nucleic acid samples 审中-公开
标题翻译：用于制备核酸样品的方法和试剂盒
公开(公告)号：US20070020654A1
公开(公告)日：2007-01-25
申请号：US11419459
申请日：2006-05-19
申请人： John Blume , Yanxiang Cao , Kyle Cole , Kai Wu , Charles Miyada
发明人： John Blume , Yanxiang Cao , Kyle Cole , Kai Wu , Charles Miyada
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q2531/143 , C12Q2525/179 , C12Q2521/531 , C12Q2521/131
摘要： The present invention provides methods for preparing nucleic acid samples. The methods of the present invention are particularly amenable for preparing samples that substantially represent the whole transcripts. In some aspects the methods include a step of reducing the amount of ribosomal RNA in a total RNA sample prior to amplification. In preferred aspects single stranded sense strand cDNA is generated, labeled and hybridized to arrays of probes. The method is particularly suitable to use with microarray based expression analysis.
摘要翻译：本发明提供了制备核酸样品的方法。本发明的方法特别适于制备基本上代表整个转录物的样品。在一些方面，所述方法包括在扩增前减少总RNA样品中的核糖体RNA的量的步骤。在优选的方面，产生单链有义链cDNA，标记并与探针阵列杂交。该方法特别适用于基于微阵列的表达分析。

4. 发明申请

US20050123956A1 Methods for modifying DNA for microarray analysis 审中-公开
标题翻译：修改DNA用于微阵列分析的方法
公开(公告)号：US20050123956A1
公开(公告)日：2005-06-09
申请号：US10951983
申请日：2004-09-27
申请人： John Blume , Yanxiang Cao , Glenn McGall , Kyle Cole , Fredrick Christians , Kai Wu , Linda Hsie , Charles Miyada , Anthony Barone , Vivi Truong
发明人： John Blume , Yanxiang Cao , Glenn McGall , Kyle Cole , Fredrick Christians , Kai Wu , Linda Hsie , Charles Miyada , Anthony Barone , Vivi Truong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/683 , C12Q2565/501 , C12Q2525/119 , C12Q2521/531
摘要： In one aspect of the invention, methods and compositions are provided for fragmenting nucleic acid samples. Fragmented nucleic acid samples may be used for hybridization with microarrays.
摘要翻译：在本发明的一个方面，提供了用于片段化核酸样品的方法和组合物。分离的核酸样品可用于与微阵列杂交。

5. 发明授权

US09261294B2 Joint heating system of gas combined cycle and solar power and dispatching method thereof 有权
标题翻译：气体联合循环和太阳能联合供热系统及其调度方法
公开(公告)号：US09261294B2
公开(公告)日：2016-02-16
申请号：US13809889
申请日：2011-12-31
申请人： Hongyu Long , Kai Wu , Yulong Yang
发明人： Hongyu Long , Kai Wu , Yulong Yang
IPC分类号： F24H9/20 , F24H4/00 , F02C3/28 , F02C6/18 , H02J3/38 , F24D19/10 , F24D15/04
CPC分类号： F24H9/2007 , F02C3/28 , F02C6/18 , F24D15/04 , F24D19/1039 , F24D19/1048 , F24D2200/12 , F24H4/00 , F24H2240/00 , H02J3/381 , H02J3/383 , Y02B10/20 , Y02B10/70 , Y02B30/12 , Y02E10/563 , Y02E20/14
摘要： A joint heating system of gas combined cycle and solar power and a dispatching method thereof, the user adopts two ways of the hot water radiator and the heat pump to supply heat, wherein the hot water comes from the gas combined cycle units, the electric power comes from the combination of the gas combined cycle units and the solar power generation units, and after detecting the power supplying and power consumption of the user in a historical time period by the comprehensive dispatching and controlling device, a future time period is predicted, and then dispatching is processed on the basis.
摘要翻译：气体联合循环和太阳能发电联合加热系统及其调度方法，用户采用热水散热器和热泵两种方式供热，其中热水来自气体联合循环装置，电力来自气体联合循环单元和太阳能发电单元的组合，并且在通过综合调度和控制装置检测用户在历史时间段内的供电和功耗之后，预测将来的时间段，然后在此基础上进行调度处理。

6. 发明授权

US08669784B1 Programmable pulse generator using inverter chain 有权
标题翻译：可编程脉冲发生器采用逆变链
公开(公告)号：US08669784B1
公开(公告)日：2014-03-11
申请号：US13448176
申请日：2012-04-16
申请人： Kai Wu
发明人： Kai Wu
IPC分类号： H03K19/00 , H03K3/017
CPC分类号： H03K5/05
摘要： In one embodiment, a method includes generating a first signal based on a clock signal and generating a second signal based on a programmable delayed clock signal. The method then generates a reset signal based on the first signal and the second signal. The clock signal is delayed using an inverter chain to generate a delayed version of the clock signal. An output signal is generated based on the delayed version of the clock signal and the reset signal. When a pulse width of the output signal is greater than a data duration determined from the clock signal, the pulse width of the output signal is reset to the pulse width of the data duration.
摘要翻译：在一个实施例中，一种方法包括基于时钟信号产生第一信号并基于可编程延迟时钟信号产生第二信号。该方法然后基于第一信号和第二信号产生复位信号。使用反相器链延迟时钟信号以产生时钟信号的延迟版本。基于延迟版本的时钟信号和复位信号产生输出信号。当输出信号的脉冲宽度大于从时钟信号确定的数据持续时间时，输出信号的脉冲宽度被重置为数据持续时间的脉冲宽度。

7. 发明申请

US20120322683A1 ENZYMATIC SIGNAL GENERATION AND DETECTION OF BINDING COMPLEXES IN STATIONARY FLUIDIC CHIP 有权
标题翻译：固定信号生成和检测固定液流芯片中的结合复合物
公开(公告)号：US20120322683A1
公开(公告)日：2012-12-20
申请号：US13543488
申请日：2012-07-06
申请人： David J. Liu , Kai Wu , Xing Su
发明人： David J. Liu , Kai Wu , Xing Su
IPC分类号： G01N33/558 , G01N33/53 , C40B30/04 , G01N33/573 , G01N21/78 , G01N27/26 , G01N21/00 , G01N21/76 , C12Q1/70 , B82Y15/00
CPC分类号： G01N21/658 , B01L3/50273 , B01L3/502761 , B01L2300/0654 , B01L2400/043 , B01L2400/086 , B82Y15/00 , B82Y30/00 , G01N21/255 , G01N21/6428 , G01N21/645 , G01N21/76 , G01N33/54333 , G01N33/587
摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
摘要翻译：本发明的一个实施例涉及用于检测样品中的分析物的装置。该装置包括流体网络和集成电路部件。流体网络包括样品区，清洁区和检测区。流体网络包含磁性颗粒和/或信号颗粒。引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

8. 发明申请

US20120231626A1 FORMATION OF LINER AND BARRIER FOR TUNGSTEN AS GATE ELECTRODE AND AS CONTACT PLUG TO REDUCE RESISTANCE AND ENHANCE DEVICE PERFORMANCE 有权
标题翻译：形成用于电极电极和接触电极的衬里和障板，以降低电阻和增强器件性能
公开(公告)号：US20120231626A1
公开(公告)日：2012-09-13
申请号：US13415727
申请日：2012-03-08
申请人： SANG-HYEOB LEE , Sang Ho Yu , Kai Wu
发明人： SANG-HYEOB LEE , Sang Ho Yu , Kai Wu
IPC分类号： H01L21/768 , H01L21/285
CPC分类号： C23C16/0272 , C23C16/34 , C23C16/45529 , C23C16/56 , H01L21/28556 , H01L21/76846 , H01L27/10891
摘要： The invention provides a method of forming a film stack on a substrate, comprising performing a silicon containing gas soak process to form a silicon containing layer over the substrate, reacting with the silicon containing layer to form a tungsten silicide layer on the substrate, depositing a tungsten nitride layer on the substrate, subjecting the substrate to a nitridation treatment using active nitrogen species from a remote plasma, and depositing a conductive bulk layer directly on the tungsten nitride layer.
摘要翻译：本发明提供了一种在衬底上形成膜堆叠的方法，包括进行含硅气体保温工艺以在衬底上形成含硅层，与含硅层反应以在衬底上形成硅化钨层，沉积氮化硅层，使用来自远程等离子体的活性氮物质进行氮化处理，并将导电体层直接沉积在氮化钨层上。

9. 发明授权

US08211799B2 Atomic layer deposition of tungsten materials 有权
标题翻译：原子层沉积钨材料
公开(公告)号：US08211799B2
公开(公告)日：2012-07-03
申请号：US13160378
申请日：2011-06-14
申请人： Amit Khandelwal , Madhu Moorthy , Avgerinos V. Gelatos , Kai Wu
发明人： Amit Khandelwal , Madhu Moorthy , Avgerinos V. Gelatos , Kai Wu
IPC分类号： H01L21/44
CPC分类号： C23C16/0281 , C23C16/08 , C23C16/14 , C23C16/45525 , H01L21/28556 , H01L21/28562 , H01L21/76843 , H01L21/76855 , H01L21/76856 , H01L21/7687 , H01L21/76876 , H01L21/76877 , H01L2221/1089
摘要： Embodiments of the invention provide a method for depositing tungsten-containing materials. In one embodiment, a method includes forming a tungsten nucleation layer over an underlayer disposed on the substrate while sequentially providing a tungsten precursor and a reducing gas into a process chamber during an atomic layer deposition (ALD) process and depositing a tungsten bulk layer over the tungsten nucleation layer, wherein the reducing gas contains hydrogen gas and a hydride compound (e.g., diborane) and has a hydrogen/hydride flow rate ratio of about 500:1 or greater. In some examples, the method includes flowing the hydrogen gas into the process chamber at a flow rate within a range from about 1 slm to about 20 slm and flowing a mixture of the hydride compound and a carrier gas into the process chamber at a flow rate within a range from about 50 sccm to about 500 sccm.
摘要翻译：本发明的实施方案提供了一种沉积含钨材料的方法。在一个实施例中，一种方法包括在设置在衬底上的底层上形成钨成核层，同时在原子层沉积（ALD）工艺期间依次提供钨前体和还原气体到处理室中，并在其上沉积钨体积层钨成核层，其中所述还原气体包含氢气和氢化物化合物（例如乙硼烷），并且具有约500:1或更高的氢/氢化物流速比。在一些实例中，该方法包括以约1slm至约20slm的流速将氢气流入处理室，并将氢化物化合物和载气的混合物以流速流动到处理室中在约50sccm至约500sccm的范围内。

10. 发明申请

US20120140239A1 METHOD FOR MONITORING THIN FILM DEPOSITION USING DYNAMIC INTERFEROMETER 审中-公开
标题翻译：使用动态干涉仪监测薄膜沉积的方法
公开(公告)号：US20120140239A1
公开(公告)日：2012-06-07
申请号：US13152979
申请日：2011-06-03
申请人： Cheng-Chung LEE , Kai Wu
发明人： Cheng-Chung LEE , Kai Wu
IPC分类号： G01B9/02
CPC分类号： G01B11/0675 , G01B9/02021 , G01B9/02065 , G01B9/02081 , G01B9/0209 , G01B2290/70 , G01N21/45
摘要： A method for real-time monitoring thin film deposition using a dynamic interferometer is revealed. An optical monitoring extracting the temporal phase change of the reflection coefficient of the deposition film stacks. The dynamic interferometer, which gets rid of the influence of vibration and air turbulence, was used in the method to directly detect fluctuating phase of a deposition film stack. Combing with the reflectance or transmittance measurements, the real-time reflection coefficient under normal incidence of monitoring light can be found as well as optical admittance for enhancing the error compensation of the thin film deposition.
摘要翻译：揭示了使用动态干涉仪实时监测薄膜沉积的方法。提取沉积膜堆叠的反射系数的时间相位变化的光学监测。用于直接检测沉积膜叠层波动相位的方法中，摆脱了振动和空气湍流影响的动态干涉仪。配合反射率或透射率测量，可以发现在监控光的正常入射下的实时反射系数以及用于增强薄膜沉积的误差补偿的光学导纳。

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