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    • 8. 发明授权
    • Single-step, solid-state competitive immunoassay
    • 单步,固态竞争性免疫测定
    • US5891658A
    • 1999-04-06
    • US671378
    • 1996-06-27
    • Stanley M. KlainerStephen L. CoulterGeoffrey F. Hewitt
    • Stanley M. KlainerStephen L. CoulterGeoffrey F. Hewitt
    • G01N33/543G01N33/53G01N33/552
    • G01N33/54386G01N33/54373Y10S435/808Y10S436/805
    • Simplicity, sensitivity and versatility of optical sensors based on competitive immunoassays using antibody-antigen reactions are achieved by solid-state, single-step reactions which permit accurate sensitive qualitative and quantitative information to be obtained without human participation. All of the chemistry-biochemistry is an inherent part of the sensor. A direct reaction occurs when the sample (antigen) is brought in contact with the sensor. The sensitivity of the competitive immunoassay optical sensor is controlled and increased by selecting a tag for the antigen or altering the attachment of a tag to an antigen so that the binding of tagged antigen to an antibody is decreased relative to the binding of untagged antigen to the antibody. The user can vary size, molecular weight and geometric configuration of the tagged antigen. This can be accomplished by selecting the proper tag or by attaching the indicator material to the antigen through a spacer or by attaching the tag directly to the antigen and attaching a compound of proper molecular weight and size elsewhere. Pretreatment of the substrate to which the antibody-bound tagged antigen is immobilized to block the surface from unwanted interferences and the use of optical isolation increases sensitivity. If the tag cannot be attached to the antigen, it is attached to the antibody. Attaching the controlled size, molecular weight tag to either the antigen or antibody permits the analysis of species not normally measureable by competitive immunoassay, thus increasing the versatility of the method.
    • 基于使用抗体 - 抗原反应的竞争性免疫测定法的光学传感器的简单性,灵敏度和通用性通过固态单步反应实现,其允许在没有人参与的情况下获得准确的敏感性定性和定量信息。 所有的化学生物化学是传感器的固有部分。 当样品(抗原)与传感器接触时,会发生直接反应。 竞争性免疫测定光学传感器的灵敏度通过选择抗原标签或改变标签对抗原的附着来控制和增加,使得标记的抗原与抗体的结合相对于未标记的抗原与 抗体。 用户可以改变标记抗原的大小,分子量和几何构型。 这可以通过选择适当的标签或通过间隔物将指示剂材料附着到抗原上或通过将标签直接附着在抗原上并将其它地方具有合适的分子量和大小的化合物来实现。 固定有抗体结合标记的抗原的底物的预处理以阻止表面免受不想要的干扰,并且使用光学隔离增加灵敏度。 如果标签不能连接到抗原上,则其与抗体连接。 将受控大小的分子量标签附加到抗原或抗体上允许通过竞争性免疫测定通常不能测量的物种的分析,从而增加该方法的通用性。
    • 10. 发明授权
    • Optical sensor for the detection of ice formation and other chemical
species
    • 用于检测冰层和其他化学物质的光学传感器
    • US4913519A
    • 1990-04-03
    • US164172
    • 1988-03-04
    • Stanley M. KlainerFred P. Milanovich
    • Stanley M. KlainerFred P. Milanovich
    • G01N21/41C03C13/00G01N21/43G02B6/02
    • G01N21/431C03C13/008G02B6/02
    • An ice sensor for the remote rapid indication of ice formation or the presence of ice is a fiber optic "switch", activated by ice but not by water, and based on the difference in optical properties between water and ice. The approach is to construct a "fiber optic" which itself is the ice sensor. The fiber optic sensor (FOS) is designed so that no light is transmitted when water is present but as soon as ice begins to form, light is relayed. Thus ice switches on the light- In addition, limited quantitative information can be made available on the rate of ice formation. Alternatively the sensor can be formed of another type optical waveguide instead of an optical fiber. The ice sensor is formed by placing spaced stripes of a clad material on a fiber optic core, or other waveguide structure, where the clad has a refractive index close to ice and the core has an index greater than the clad but less than water. It is best to index match the core to the clad for optimal transmission when ice forms in the gaps between the clad stripes. The advantages of the ice sensor include: specific, sensitive, real-time response, small, light weight, inexpensive, requires no line-of-sight, EMI immune, rugged and flexible. The instrumentation needed to operate the ice FOS is simple, small, light weight, inexpensive, easy to operate, battery powered - optional, rugged, reliable and amenable to telemetry of information.
    • 用于远程快速指示冰层或存在冰的冰传感器是由冰而不是水激活的光纤“开关”,并且基于水和冰之间的光学性质的差异。 该方法是构造一个本身是冰传感器的“光纤”。 光纤传感器(FOS)被设计成当存在水时不发射光,但一旦冰开始形成,光被中继。 因此,在冰上开启冰。此外,可以根据冰的形成速率提供有限的定量信息。 或者,传感器可以由另一种类型的光波导代替光纤形成。 冰传感器通过将包覆材料的间隔条纹放置在光纤芯或其它波导结构上而形成,其中包层的折射率接近于冰,并且芯的折射率大于包层但小于水。 当包层条纹之间的间隙中形成冰时,最好将核心与包层匹配,以获得最佳传播。 冰传感器的优点包括:具体,灵敏,实时响应小,重量轻,价格便宜,无需视距,EMI免疫,坚固耐用,灵活。 操作冰FOS所需的仪器简单,体积小,重量轻,价格低廉,易于操作,电池供电 - 可选,坚固耐用,可靠,易于遥测信息。