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    • 6. 发明授权
    • Method of producing .alpha.-L-aspartyl-L-phenylalanine methyl ester
hydrochloride
    • 生产α-L-天冬氨酰-L-苯丙氨酸甲酯盐酸盐的方法
    • US5225587A
    • 1993-07-06
    • US864133
    • 1992-04-06
    • Katsumi SugiyamaKiyo AdachiToshihide Yukawa
    • Katsumi SugiyamaKiyo AdachiToshihide Yukawa
    • C07K5/072C07K5/075
    • C07K5/0613
    • A method of purifying .alpha.-L-aspartyl-L-phenylalanine methyl ester as a hydrochloride salt thereof which comprises:i) contacting a mixture of .alpha.-L-aspartyl-L-phenylanaline methyl ester and .beta.-L-aspartyl-L-phenylalanine methyl ester with an aqueous medium containing (a) hydrogen chloride in an amount not more than ca. 2 mol., per liter of said aqueous medium, but not less than 1 mol., per mole of said of said mixture of .alpha.-L-aspartyl-L-phenylalanine methyl ester and .beta.-L-aspartyl-L-phenylalanine methyl ester, and (b) an inorganic chloride selected from the group consisting of NaCl, KCl, NH.sub.4 Cl, CaCl.sub.2, and ZnCl.sub.2 in an amount of at least ca. 50 g., per liter of said aqueous medium, to form crystals of .alpha.-L-aspartyl-L-phenylalanine methyl ester hydrochloride, andii) recovering the .alpha.-L-aspartyl-L-phenylalanine methyl ester hydrochloride crystals therefrom.
    • 纯化α-L-天冬氨酰基-L-苯丙氨酸甲酯盐酸盐的方法,其包括:i)使α-L-天冬氨酰基-L-苯亚胺甲酯与β-L-天冬氨酰基-L-苯丙氨酸的混合物 甲酯与含有(a)氯化氢的含水介质的量不大于约 每摩尔所述所述α-L-天冬氨酰基-L-苯丙氨酸甲酯和β-L-天冬氨酰-L-苯丙氨酸甲酯的混合物每升所述水性介质2摩尔,但不小于1摩尔 ,和(b)选自NaCl,KCl,NH 4 Cl,CaCl 2和ZnCl 2的无机氯化物,其量至少约为。 50升,每升所述水性介质中,形成α-L-天冬氨酰基-L-苯丙氨酸甲酯盐酸盐的晶体,和ii)从其中回收α-L-天冬氨酰基-L-苯丙氨酸甲酯盐酸盐晶体。
    • 10. 发明授权
    • Method for recovering .alpha.-L-aspartyl-L-phenylalanine methyl ester
    • α-L-天冬氨酰-L-苯丙氨酸甲酯的回收方法
    • US4822907A
    • 1989-04-18
    • US56907
    • 1987-06-03
    • Katsumi SugiyamaTakashi YamashitaToshihide Yukawa
    • Katsumi SugiyamaTakashi YamashitaToshihide Yukawa
    • C07K1/14C07K1/26C07K5/072C07K5/075C07C103/52
    • C07K5/0613
    • A process for recovering .alpha.-L-aspartyl-L-phenylalanine methyl ester from a solution containing various methyl ester or demethylated derivatives of .alpha.-L-aspartyl-L-phenylalanine methyl ester and chloride ions is disclosed. This process uses a reverse osmosis membrane having a specific chloride inhibition rate to desalt and concentrate a solution containing a specified ratio of .delta.-L-aspartyl-L-phenylalanine methyl ester to its derivatives until a specific weight ratio of organic chloride ion to the sum of .alpha.-L-aspartyl-L-phenylalanine methyl ester and its derivatives is obtained. This desalted and concentrated solution is then contacted with an aqueous solvent containing methanol and hydrochloride acid to recover high yields of .alpha.-L-aspartyl-L-phenylalanine methyl ester hydrochloride.
    • 公开了从含有α-L-天冬氨酰-L-苯丙氨酸甲酯和氯离子的各种甲酯或去甲基化衍生物的溶液中回收α-L-天冬氨酰基-L-苯丙氨酸甲酯的方法。 该方法使用具有特定氯化物抑制速率的反渗透膜脱盐,并将含有特定比例的δ-L-天冬氨酰-L-苯丙氨酸甲酯的溶液浓缩至其衍生物,直到有机氯离子与总和的比重 的α-L-天冬氨酰基-L-苯丙氨酸甲酯及其衍生物。 然后将该脱盐浓缩溶液与含有甲醇和盐酸的含水溶剂接触,以回收高产率的α-L-天冬氨酰基-L-苯丙氨酸甲酯盐酸盐。