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    • 1. 发明授权
    • Two-dimensional precision table
    • 二维精密表
    • US4129291A
    • 1978-12-12
    • US818743
    • 1977-07-25
    • Shigeo KatoOsamu MoritaSadao Matuoka
    • Shigeo KatoOsamu MoritaSadao Matuoka
    • B23Q1/26B23Q1/38B23Q1/62B24B47/02F16C29/02B23Q1/02
    • F16C32/06B23Q1/38B23Q1/621F16C29/025
    • A precision table for a machine tool or the like, which table mounts a workpiece and is adapted to be selectively moved or slid in two directions by a low viscosity bearing such as an externally pressurized air bearing. The bearing includes at least one guide of a substantially J-shaped cross-sectional configuration with a jet arrangement being provided within the guide for jetting a pressurized gas through at least one orifice toward at least one internal surface of the guide. The jet arrangement is disposed within the guide which is fixedly arranged on a flat surface of a bed so that the table is supported by an air film in a clearance between the jet arrangement and portions of the guide.
    • 用于机床等的精密工作台,该工作台安装工件并适于通过诸如外部加压空气轴承的低粘度轴承在两个方向上选择性地移动或滑动。 所述轴承包括基本上为J形的横截面构造的至少一个引导件,其中喷射装置设置在引导件内,用于通过至少一个孔朝向引导件的至少一个内表面喷射加压气体。 喷射装置设置在固定地布置在床的平坦表面上的引导件内,使得工作台由喷气装置和引导件的一部分之间的间隙中的空气膜支撑。
    • 9. 发明申请
    • METHOD OF PRODUCING FUSED PROTEIN
    • 生产融合蛋白的方法
    • US20110065149A1
    • 2011-03-17
    • US12310258
    • 2007-08-21
    • Shigeo KatoYoichi KumadaTomomi KawasakiYasufumi Kikuchi
    • Shigeo KatoYoichi KumadaTomomi KawasakiYasufumi Kikuchi
    • C12P21/00C07H21/04C12N15/63C12N1/21C07H21/00
    • C12N15/62C07K16/44C07K2317/622
    • Described herein is a polynucleotide encoding a fusion protein comprising two or more polypeptide domains and a polypeptide linker joining the domains, wherein the sequence of the polynucleotide encoding the polypeptide linker is selected such that when the mRNA transcribed from the polynucleotide is translated in a host cell transfected with the polynucleotide, the translation rate of the mRNA region encoding the polypeptide linker is slower than the translation rate of the mRNA region encoding the polypeptide domain immediately upstream thereof. Also provided are a vector transfected with the polynucleotide of the present invention so that the polynucleotide can be expressed in the host cell; a host cell transformed by that vector; and a process for producing a fusion protein comprising culturing the host cell, and recovering the fusion protein thus produced.
    • 本文描述了编码融合蛋白的多核苷酸,其包含两个或多个多肽结构域和连接结构域的多肽接头,其中选择编码多肽接头的多核苷酸序列,使得当从多核苷酸转录的mRNA在宿主细胞中翻译时 用多核苷酸转染,编码多肽接头的mRNA区的翻译速率比编码紧邻其上游的多肽结构域的mRNA区的翻译速度慢。 还提供了用本发明的多核苷酸转染的载体,使得多核苷酸可以在宿主细胞中表达; 由该载体转化的宿主细胞; 以及生产融合蛋白的方法,包括培养宿主细胞,并回收由此产生的融合蛋白。