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1. 发明授权

US07022529B2 Particles for diagnostic and therapeutic use 有权
公开(公告)号：US07022529B2
公开(公告)日：2006-04-04
申请号：US10745972
申请日：2003-12-24
申请人： Sharat Singh , John S. Pease , Jacqueline Sadakian , Daniel B. Wagner , Edwin F. Ullman
发明人： Sharat Singh , John S. Pease , Jacqueline Sadakian , Daniel B. Wagner , Edwin F. Ullman
IPC分类号： G01N33/543
CPC分类号： G01N33/587 , C12Q1/6816 , Y10T436/10 , C12Q2563/155 , C12Q2523/319
摘要： Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 μm in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected. The presence and amount of the effect is related to the presence and amount of the analyte in the medium. Also disclosed are kits for use in an assay.

2. 发明授权

US07709273B2 Particles for diagnostic and therapeutic use 有权
标题翻译：用于诊断和治疗用途的颗粒
公开(公告)号：US07709273B2
公开(公告)日：2010-05-04
申请号：US11273275
申请日：2005-11-14
申请人： Sharat Singh , John S. Pease , Jacqueline Sadakian , Daniel B. Wagner , Edwin F. Ullman
发明人： Sharat Singh , John S. Pease , Jacqueline Sadakian , Daniel B. Wagner , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N31/00
CPC分类号： G01N33/587 , C12Q1/6816 , Y10T436/10 , C12Q2563/155 , C12Q2523/319
摘要： Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 μm in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected. The presence and amount of the effect is related to the presence and amount of the analyte in the medium. Also disclosed are kits for use in an assay.
摘要翻译：公开了方法，组合物和试剂盒。组合物是发光的并且包含其中溶解有光敏化合物的聚合物基质。该组合物具有以下特征：在光活性化合物活化后，强度降低20倍的任何时间的发光强度的降低率与发光强度成比例。在一个实施方案中，聚合物基质包含直径约20nm至约100μm的颗粒，其与特定结合对构件结合。颗粒通常包含其中溶解约1至约20重量％的掺杂剂的聚合物基质。该组合物可用于测定分析物的方法中。提供的组合包括（1）怀疑含有分析物的介质，（2）和上述组合物。光活性物质被激活，并且检测到激活对组合的光学性质的影响。影响的存在和数量与介质中分析物的存在和数量有关。还公开了用于测定的试剂盒。

3. 发明授权

US06703248B1 Particles for diagnostic and therapeutic use 有权
公开(公告)号：US06703248B1
公开(公告)日：2004-03-09
申请号：US09465065
申请日：1999-12-15
申请人： Sharat Singh , John S. Pease , Jacqueline Sadakian , Daniel B. Wagner , Edwin F. Ullman
发明人： Sharat Singh , John S. Pease , Jacqueline Sadakian , Daniel B. Wagner , Edwin F. Ullman
IPC分类号： G01N33553
CPC分类号： G01N33/587 , C12Q1/6816 , Y10T436/10 , C12Q2563/155 , C12Q2523/319
摘要： Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 &mgr;m in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected. The presence and amount of the effect is related to the presence and amount of the analyte in the medium. Also disclosed are kits for use in an assay.

4. 发明授权

US06406913B1 Assay method utilizing induced luminescence 失效
标题翻译：利用诱导发光的测定方法
公开(公告)号：US06406913B1
公开(公告)日：2002-06-18
申请号：US08471130
申请日：1995-06-06
申请人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
发明人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
IPC分类号： G01T1161
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译：公开了用于确定怀疑含有分析物的介质中的分析物的方法。一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。活化的化学发光化合物随后产生光。所产生的光量与介质中分析物的量有关。优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。还公开了组合物和试剂盒。

5. 发明授权

US5618732A Method of calibration with photoactivatable chemiluminescent matrices 失效
标题翻译：用光活化化学发光基质校准的方法
公开(公告)号：US5618732A
公开(公告)日：1997-04-08
申请号：US434617
申请日：1995-05-04
申请人： John S. Pease , Hrair Kirakossian , Daniel B. Wagner , Edwin F. Ullman
发明人： John S. Pease , Hrair Kirakossian , Daniel B. Wagner , Edwin F. Ullman
IPC分类号： G01N33/532 , G01N21/78 , G01N33/58 , G01N21/64 , G01N21/76
CPC分类号： G01N33/582 , Y10S435/81 , Y10S436/829 , Y10S436/905 , Y10T436/10
摘要： Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
摘要翻译：公开了标记材料的方法。所述方法包括与材料（a）结合，所述光敏剂能够在产生单线态氧的照射时和（b）能够被单线态氧活化的化学发光化合物，其中光敏剂和化学发光化合物掺入颗粒基质或非均相中，颗粒状固体基质。颗粒基质可以是固体或流体。这些方法允许产生延迟发光，这可以在照射基质时实现。该方法可用于确定怀疑含有分析物的介质中的分析物。一种方法包括使怀疑含有分析物的培养基相对于分析物的存在形成特异性结合对（sbp）成员的复合物的条件，以及通过使用标记a来确定sbp成员复合物是否形成具有化学发光和光敏剂性质的单一组合物。激活光敏剂时，产生单线态氧并激活化学发光性能。还公开了组合物和试剂盒。

6. 发明授权

US06251581B1 Assay method utilizing induced luminescence 失效
标题翻译：利用诱导发光的测定方法
公开(公告)号：US06251581B1
公开(公告)日：2001-06-26
申请号：US07704569
申请日：1991-05-22
申请人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
发明人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
IPC分类号： C12Q100
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译：公开了用于确定怀疑含有分析物的介质中的分析物的方法。一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。活化的化学发光化合物随后产生光。所产生的光量与介质中分析物的量有关。优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。还公开了组合物和试剂盒。

7. 发明授权

US5709994A Photoactivatable chemiluminescent matrices 失效
标题翻译：光活化化学发光基质
公开(公告)号：US5709994A
公开(公告)日：1998-01-20
申请号：US470862
申请日：1995-06-06
申请人： John S. Pease , Hrair Kirakossian , Daniel B. Wagner , Edwin F. Ullman
发明人： John S. Pease , Hrair Kirakossian , Daniel B. Wagner , Edwin F. Ullman
IPC分类号： G01N33/532 , G01N21/78 , G01N33/58 , G01N33/52 , C09K11/00 , C12Q1/00 , C12Q1/68
CPC分类号： G01N33/582 , Y10S435/81 , Y10S436/829 , Y10S436/905 , Y10T436/10
摘要： Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
摘要翻译：公开了标记材料的方法。所述方法包括与材料（a）结合，所述光敏剂能够在产生单线态氧的照射时和（b）能够被单线态氧活化的化学发光化合物，其中光敏剂和化学发光化合物掺入颗粒基质或非均相中，颗粒状固体基质。颗粒基质可以是固体或流体。这些方法允许产生延迟发光，这可以在照射基质时实现。该方法可用于确定怀疑含有分析物的介质中的分析物。一种方法包括使怀疑含有分析物的培养基相对于分析物的存在形成特异性结合对（sbp）成员的复合物的条件，以及通过使用标记a来确定sbp成员复合物是否形成具有化学发光和光敏剂性质的单一组合物。激活光敏剂时，产生单线态氧并激活化学发光性能。还公开了组合物和试剂盒。

8. 发明授权

US5340716A Assay method utilizing photoactivated chemiluminescent label 失效
标题翻译：使用光活化化学发光标记的测定方法
公开(公告)号：US5340716A
公开(公告)日：1994-08-23
申请号：US718490
申请日：1991-06-20
申请人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
发明人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
IPC分类号： G01N33/569 , G01N33/58 , C12Q1/00
CPC分类号： G01N33/582 , G01N33/56977 , G01N33/58 , G01N33/585
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises providing (1) a medium suspected of containing the analyte, (2) a label reagent comprising a first specific binding pair (sbp) member associated with a photochemically activatable chemiluminescent compound wherein the first sbp member is capable of binding to the analyte or to a second sbp member to form a complex related to the presence of the analyte. The method further comprises photochemically activating the chemiluminescent compound. The amount of luminescence generated by the chemiluminescent compound is detected. The amount thereof is related to the amount of analyte in the medium. Compositions and kits are also disclosed.
摘要翻译：公开了用于确定怀疑含有分析物的介质中的分析物的方法。一种方法包括提供（1）怀疑含有分析物的培养基，（2）标记试剂，其包含与光化学活化的化学发光化合物相关的第一特异性结合对（sbp）成员，其中第一sbp成员能够结合分析物或第二sbp成员以形成与分析物的存在有关的复合物。该方法还包括光化学活化化学发光化合物。检测由化学发光化合物产生的发光量。其量与培养基中分析物的量有关。还公开了组合物和试剂盒。

9. 发明授权

US06632606B1 Methods for single nucleotide polymorphism detection 失效
标题翻译：单核苷酸多态性检测方法
公开(公告)号：US06632606B1
公开(公告)日：2003-10-14
申请号：US09592053
申请日：2000-06-12
申请人： Edwin F. Ullman , Sharat Singh
发明人： Edwin F. Ullman , Sharat Singh
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q2565/519 , C12Q2535/125 , C12Q2563/179 , C12Q2563/131
摘要： Methods and compositions are provided for determining large numbers of single nucleotide polymorphisms in target DNA employing particles having (1) primers complementary to sequences in the target DNA where the next succeeding 3′-nucleotide is a potential single nucleotide polymorphism and coding composition members, where the members are unique for each primer, and (2) differentially labeled terminating nucleotides, where the label permits separation of the terminating nucleotides. Desirably the particles are separated into groups having a common prevalent next succeeding nucleotide. The particles and target DNA are combined under nucleotide extending conditions, the particles separated into groups in accordance with the terminating nucleotide and the coding members identified, so that one knows the sequence and the single nucleotide polymorphism. Various protocols are provided for the determination.
摘要翻译：提供了用于使用具有（1）与目标DNA中的序列互补的引物，其中下一个后续的3'-核苷酸是潜在的单核苷酸多态性和编码组成成员的粒子来确定靶DNA中大量单核苷酸多态性的方法和组合物，其中成员对于每个引物是独特的，和（2）差异标记的终止核苷酸，其中标记允许分离终止的核苷酸。理想地，将颗粒分离成具有普通的下一个后续核苷酸的基团。颗粒和靶DNA在核苷酸延伸条件下合并，根据终止的核苷酸和鉴定的编码成员将颗粒分成组，以便知道序列和单核苷酸多态性。提供各种协议用于确定。

10. 发明授权

US06180354B2 Metal chelate containing compositions for use in chemiluminescent assays 失效
标题翻译：含金属螯合物的组合物用于化学发光测定
公开(公告)号：US06180354B2
公开(公告)日：2001-01-30
申请号：US08480430
申请日：1995-06-06
申请人： Sharat Singh , Edwin F. Ullman
发明人： Sharat Singh , Edwin F. Ullman
IPC分类号： G01N3353
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , G01N33/533 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/968 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10T436/13
摘要： Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.
摘要翻译：公开了包含（a）金属螯合物的组合物，其中金属选自铕，铽，镝，钐锇和钌至少六配位状态，和（b）具有被两个芳基取代的双键的化合物基团，氧原子和选自氧，硫和氮的原子，其中一个芳基相对于另一个赋予电子。这种组合物优选掺入胶乳颗粒材料中。还公开了用于测定怀疑含有分析物的培养基中的分析物的方法和试剂盒。所述方法和试剂盒采用如上所述的组合物作为一种组分。

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