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1. 发明授权

US07309568B2 Method in inspecting DNA and apparatus therefor 有权
标题翻译：检查DNA及其仪器的方法
公开(公告)号：US07309568B2
公开(公告)日：2007-12-18
申请号：US10229204
申请日：2002-08-26
申请人： Yoshitada Oshida , Satoshi Takahashi , Kenji Yasuda , Taisaku Seino
发明人： Yoshitada Oshida , Satoshi Takahashi , Kenji Yasuda , Taisaku Seino
IPC分类号： C12Q1/68 , C12M1/00 , B32B5/02 , G01N21/00
CPC分类号： G01N21/6428 , G01J3/4406 , G01N21/6452
摘要： A method and apparatus of stably obtaining fluorescent images of two or more fluorescent samples includes disposing the samples onto compartments defined on a substrate. The compartments are sequentially irradiated with an exciting light where the intensity varies. A value of fluorescence as generated from each of the samples is determined, and a fluorescent image is obtained based on the value of fluorescence.
摘要翻译：稳定地获得两个或多个荧光样品的荧光图像的方法和装置包括将样品设置在限定在基底上的隔室上。这些隔室用强度变化的激发光依次照射。确定从每个样品产生的荧光值，并且基于荧光值获得荧光图像。

2. 发明授权

US07400753B2 Biological sample optical measuring method and biological sample optical measuring apparatus 有权
标题翻译：生物样品光学测量方法和生物样品光学测量仪器
公开(公告)号：US07400753B2
公开(公告)日：2008-07-15
申请号：US10482614
申请日：2001-07-03
申请人： Taisaku Seino , Satoshi Takahashi , Kenji Yasuda , Yoshitada Oshida
发明人： Taisaku Seino , Satoshi Takahashi , Kenji Yasuda , Yoshitada Oshida
IPC分类号： G06K9/00
CPC分类号： G01N21/6456 , G01N15/1475 , G01N21/6428 , G01N21/76 , G01N2021/1734 , G01N2021/6439 , G01N2021/6491
摘要： A fluorescent or luminescence image of at least part of a biological sample is imaged, then a transmission light image of at least part of the biological sample is imaged, and thereafter the fluorescent or luminescence image and the transmission light image are overlaid such that the identical area of the sample is coincided to display. The present invention allows detecting the accurate location of fluorescence or luminescence in a given part of the biological sample.
摘要翻译：将生物样品的至少一部分的荧光或发光图像成像，然后对生物样品的至少一部分的透射光图像进行成像，然后将荧光或发光图像和透射光图像重叠，使得相同样品的面积一致显示。本发明允许检测生物样品的给定部分中的荧光或发光的准确位置。

3. 发明授权

US06760105B2 Method and apparatus for inspecting DNA and method for detecting fluorescence 有权
标题翻译：检测DNA的方法和装置及荧光检测方法
公开(公告)号：US06760105B2
公开(公告)日：2004-07-06
申请号：US10053976
申请日：2002-01-18
申请人： Yoshitada Oshida , Satoshi Takahashi , Taisaku Seino , Kenji Yasuda
发明人： Yoshitada Oshida , Satoshi Takahashi , Taisaku Seino , Kenji Yasuda
IPC分类号： G01N2164
CPC分类号： G01N21/6452 , G01N21/6428 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441
摘要： A DNA sample added with a plurality of fluorescent marks is irradiated with excitation lights having a plurality of wavelengths and when fluorescence intensities are detected separately, the excitation light and a position on the sample are relatively changed over a desired area once to thereby detect the plurality of fluorescent marks at high speed.
摘要翻译：用多个荧光标记添加的DNA样品用具有多个波长的激发光照射，并且当单独检测荧光强度时，激发光和样品上的位置在期望的区域上相对地改变一次，从而检测多个的荧光标记。

4. 发明授权

US07064813B2 Apparatus and method for measuring micro area in specimen 失效
标题翻译：用于测量样品中微区的装置和方法
公开(公告)号：US07064813B2
公开(公告)日：2006-06-20
申请号：US10253479
申请日：2002-09-25
申请人： Satoshi Takahashi , Kenji Yasuda , Yoshitada Oshida , Taisaku Seino
发明人： Satoshi Takahashi , Kenji Yasuda , Yoshitada Oshida , Taisaku Seino
IPC分类号： G01N21/00
CPC分类号： G01N1/312
摘要： Disclosed is an apparatus for measuring a micro area in a specimen in which a reagent necessary for observation and examination of a cell smear specimen or a tissue specimen is dropped to cause a reaction for a measurement and an analysis by way of an image. The apparatus includes: a micro reaction unit that is able to select a specific micro area in the specimen and to subject the specific micro area to a reaction-operation; a unit that measures; records and displays an image of the micro area; and a control unit that controls the measuring, recording, and displaying unit. Reactions can be effected quickly on such specimens as smear cell specimens and tissue slice specimens. The application of the reagent solution can be saved. A comparison between local presence of a gene or anti-body and a cell image can be made quickly and easily.
摘要翻译：公开了一种用于测量样本中的微小区域的装置，其中用于观察和检查细胞涂片或组织样本的试剂下降以引起测量反应和通过图像进行分析。该装置包括：微反应单元，其能够选择样品中的特定微区域并使特定微区域进行反应操作; 衡量单位记录并显示微区图像; 以及控制单元，其控制测量，记录和显示单元。反应可以在诸如涂片细胞标本和组织切片标本的样品上快速实现。可以节省试剂溶液的应用。基因或抗体的局部存在与细胞图像的比较可以快速且容易地进行。

5. 发明授权

US07217573B1 Method of inspecting a DNA chip 有权
标题翻译：检查DNA芯片的方法
公开(公告)号：US07217573B1
公开(公告)日：2007-05-15
申请号：US09678652
申请日：2000-10-04
申请人： Yoshitada Oshida , Toshihiko Nakata , Tomoaki Sakata , Kenji Yasuda , Satoshi Takahashi
发明人： Yoshitada Oshida , Toshihiko Nakata , Tomoaki Sakata , Kenji Yasuda , Satoshi Takahashi
IPC分类号： G01N33/00 , G01N33/53 , C12Q1/68 , C12M3/00 , C07H21/04 , H01J3/14
CPC分类号： G01N21/6428 , G01N21/6452 , G01N21/6456 , Y10S436/80 , Y10T436/143333
摘要： A method of inspecting a DNA chip and an apparatus therefor that allow a picture to be reconstructed in the following steps: A plurality of irradiation spots are formed on a DNA probe array mounted on a stage. Then, the stage is displaced in X, Y directions so as to execute a scanning, thereby irradiating substantially all the entire surface of the DNA probe array. Next, a plurality of emitted fluorescent lights, which are generated from the plurality of irradiation spot portions on the DNA probe array, are converged and are then detected simultaneously by multi detectors. Finally, a data processing apparatus processes the detected signals, thereby reconstructing the picture.
摘要翻译：一种用于检查DNA芯片的方法及其装置，其允许以下列步骤重建图像：在安装在平台上的DNA探针阵列上形成多个照射点。然后，在X，Y方向上移位台，以进行扫描，从而基本上照射DNA探针阵列的整个表面。接下来，从DNA探针阵列上的多个照射点部分产生的多个发射荧光灯被会聚，然后由多个检测器同时检测。最后，数据处理装置处理检测到的信号，从而重建图像。

6. 发明授权

US08361784B2 Method of inspecting a DNA chip and apparatus thereof 有权
标题翻译：检查DNA芯片的方法及其装置
公开(公告)号：US08361784B2
公开(公告)日：2013-01-29
申请号：US11709120
申请日：2007-02-22
申请人： Yoshitada Oshida , Toshihiko Nakata , Tomoaki Sakata , Kenji Yasuda , Satoshi Takahashi
发明人： Yoshitada Oshida , Toshihiko Nakata , Tomoaki Sakata , Kenji Yasuda , Satoshi Takahashi
IPC分类号： C12M1/34 , G01N21/64
CPC分类号： G01N21/6428 , G01N21/6452 , G01N21/6456 , Y10S436/80 , Y10T436/143333
摘要： A DNA inspecting apparatus including driving means for relatively changing positions of the multi-spot lights and a position of the DNA chip so as to detect the fluorescent lights in such a manner that a desired area on the DNA chip is irradiated with the multi-spot lights, and a control system for determining and inspecting DNA information about the to-be-inspected DNA chip from fluorescent light intensities and fluorescent light positions of the desired area on the DNA chip, the fluorescent light intensities and the fluorescent light positions being detected by the driving means and the fluorescent light detecting means.
摘要翻译：一种DNA检查装置，包括用于相对改变多点灯位置的驱动装置和DNA芯片的位置，以便以这样的方式检测荧光，使得DNA芯片上的期望区域被多点照射灯和用于从DNA芯片上的期望区域的荧光强度和荧光灯位置确定和检查关于待检查DNA芯片的DNA信息的控制系统，荧光强度和荧光灯位置由驱动装置和荧光检测装置。

7. 发明申请

US20070154938A1 Method of inspecting a DNA chip and apparatus thereof 有权
标题翻译：检查DNA芯片的方法及其装置
公开(公告)号：US20070154938A1
公开(公告)日：2007-07-05
申请号：US11709120
申请日：2007-02-22
申请人： Yoshitada Oshida , Toshihiko Nakata , Tomoaki Sakata , Kenji Yasuda , Satoshi Takahashi
发明人： Yoshitada Oshida , Toshihiko Nakata , Tomoaki Sakata , Kenji Yasuda , Satoshi Takahashi
IPC分类号： C12Q1/68 , G01J3/42
CPC分类号： G01N21/6428 , G01N21/6452 , G01N21/6456 , Y10S436/80 , Y10T436/143333
摘要： A DNA inspecting apparatus including driving means for relatively changing positions of the multi-spot lights and a position of the DNA chip so as to detect the fluorescent lights in such a manner that a desired area on the DNA chip is irradiated with the multi-spot lights, and a control system for determining and inspecting DNA information about the to-be-inspected DNA chip from fluorescent light intensities and fluorescent light positions of the desired area on the DNA chip, the fluorescent light intensities and the fluorescent light positions being detected by the driving means and the fluorescent light detecting means.
摘要翻译：一种DNA检查装置，包括用于相对改变多点灯位置的驱动装置和DNA芯片的位置，以便以这样的方式检测荧光，使得DNA芯片上的期望区域被多点照射灯和用于从DNA芯片上的期望区域的荧光强度和荧光灯位置确定和检查关于待检查DNA芯片的DNA信息的控制系统，荧光强度和荧光灯位置由驱动装置和荧光检测装置。

8. 发明授权

US5057438A Electrophoretic antigen-antibody determination with laminate of multiple membranes 失效
标题翻译：电泳抗原 - 抗体用多层膜叠层测定
公开(公告)号：US5057438A
公开(公告)日：1991-10-15
申请号：US31665
申请日：1987-03-30
申请人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
发明人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
IPC分类号： G01N33/561
CPC分类号： G01N33/561 , Y10S436/809 , Y10S436/824
摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.
摘要翻译：通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

9. 发明授权

US4824778A Immunoassay for measuring the concentration of antigen in a sample 失效
标题翻译：用于测量样品中抗原浓度的免疫测定
公开(公告)号：US4824778A
公开(公告)日：1989-04-25
申请号：US867554
申请日：1986-05-28
申请人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
发明人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
IPC分类号： G01N33/543 , G01N33/561 , G01N33/53
CPC分类号： G01N33/561 , Y10S435/968
摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.
摘要翻译：免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质，使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

10. 发明授权

US09447447B2 Method and apparatus for testing cardiotoxicity and evaluating cardiomyocytes 有权
标题翻译：用于测试心脏毒性和评估心肌细胞的方法和装置
公开(公告)号：US09447447B2
公开(公告)日：2016-09-20
申请号：US13876992
申请日：2011-09-30
申请人： Kenji Yasuda , Tomoyuki Kaneko , Fumimasa Nomura
发明人： Kenji Yasuda , Tomoyuki Kaneko , Fumimasa Nomura
IPC分类号： C12M1/34 , C12Q1/02 , C12M1/42 , C12M1/36 , G01N33/50
CPC分类号： C12Q1/025 , C12M35/02 , C12M41/46 , C12M41/48 , G01N33/48728 , G01N33/5061 , G01N33/5088 , G01N2800/32
摘要： In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.
摘要翻译：在本发明中，将心肌细胞簇设置在透明基质上，根据细胞对施加于心肌细胞的强制脉动刺激的反应来评价心肌细胞的质量。将心肌细胞簇设置在透明基板上，并且以使得试剂作用于构成网络的细胞的方式暴露于含有试剂的液体的流动。通过测量从网络的相邻心肌细胞的比较获得的波动来评估由该试剂产生的心脏毒性的程度。

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