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1. 发明授权

US5177113A Free-standing, porous foam PVP:H.sub.2 O.sub.2 product 失效
标题翻译：独立的多孔泡沫PVP：H2O2产品
公开(公告)号：US5177113A
公开(公告)日：1993-01-05
申请号：US883617
申请日：1992-03-04
申请人： Russell B. Biss , Jeffrey M. Cohen , John J. Merianos , Paul D. Taylor
发明人： Russell B. Biss , Jeffrey M. Cohen , John J. Merianos , Paul D. Taylor
IPC分类号： A01N59/00 , A61K8/22 , A61K8/81 , A61K31/79 , A61K33/40 , A61Q17/00 , A61Q19/00 , C01B15/01 , C11D3/39
CPC分类号： A61Q17/005 , A01N59/00 , A61K31/79 , A61K33/40 , A61K8/22 , A61K8/8176 , A61Q19/00 , C01B15/01 , C11D3/394 , C11D3/3945 , A61K2800/57
摘要： A free-standing, porous foam product comprising an integral network of PVP molecules hydrogen-bonded to H.sub.2 O.sub.2 molecules, optionally including a water-soluble gelling agent; e.g. hydroxyethyl cellulose; in an amount up to 60% by weight of the product, its PVP content being about 25-90% by weight of the product, and the H.sub.2 O.sub.2 content being about 5-24% by weight. In the absence of a gelling agent, the product has a bulk density of about 0.3 to about 0.5 g/cc and a porosity of about 60-70%. When a gelling agent is present in the product, its bulk density and porosity of the product is substantially reduced and, accordingly, it has a more foam-like structure and appearance.
摘要翻译：一种独立的多孔泡沫产品，包括与H 2 O 2分子氢键合的PVP分子的整体网络，任选地包括水溶性胶凝剂; 例如羟乙基纤维素; 其量高达产品的60重量％，其PVP含量为产物重量的约25-90重量％，H 2 O 2含量为约5-24重量％。在没有胶凝剂的情况下，该产品具有约0.3至约0.5g / cc的堆积密度和约60-70％的孔隙率。当产品中存在胶凝剂时，产品的体积密度和孔隙率显着降低，因此具有更多的泡沫状结构和外观。

2. 发明授权

US5077047A Process for producing PVP-H2O2 products in the form of free-flowing powders 失效
标题翻译：以自由流动的粉末形式生产PVP-H2O2产品的方法
公开(公告)号：US5077047A
公开(公告)日：1991-12-31
申请号：US683467
申请日：1991-04-08
申请人： Russell B. Biss , Jeffrey Cohen , John J. Merianos , Paul D. Taylor
发明人： Russell B. Biss , Jeffrey Cohen , John J. Merianos , Paul D. Taylor
IPC分类号： A61K8/22 , A61K8/81 , A61K31/79 , A61K33/40 , A61Q17/00 , A61Q19/00 , C01B15/01 , C01B15/037
CPC分类号： A61K8/02 , A61K31/79 , A61K33/40 , A61K8/22 , A61K8/8176 , A61Q17/005 , A61Q19/00 , C01B15/01 , C01B15/037 , A61K2800/52 , Y10T428/2982
摘要： A process for the production of PVP-H.sub.2 O.sub.2 products in the form of free-flowing powders is described in which a fluidized bed maintained at a reaction temperature of about room temperature to 60.degree. C. is contacted with finely divided droplets of a 30 to 85% by weight aqueous H.sub.2 O.sub.2 solution. Preferably the fluidized bed temperature is about 35.degree.-45.degree. C., a 50-70% H.sub.2 O.sub.2 solution is used, and the feed rate of introduction of the H.sub.2 O.sub.2 solution is about 5-50 g/minute/kg PVP present. The PVP-H.sub.2 O.sub.2 product preferably contains about 15-24%, preferably 18-22%, H.sub.2 O.sub.2 (1:1 molar ratio) and less than about 5% water.
摘要翻译：描述了以自由流动的粉末形式生产PVP-H 2 O 2产物的方法，其中保持在约室温至60℃的反应温度的流化床与30至85℃的细分散液滴接触重量％H 2 O 2水溶液。优选地，流化床温度为约35°-45℃，使用50-70％的H 2 O 2溶液，并且H 2 O 2溶液的进料速率为约5-50g /分钟/ kg存在的PVP。 PVP-H 2 O 2产物优选含有约15-24％，优选18-22％的H 2 O 2（1:1摩尔比）和小于约5％的水。

3. 发明申请

US20100280299A1 METHOD FOR THE PRODUCTION OF ALPHA, OMEGA-OLEFINS BY USING THE COPPER CATALYZED COUPLING REACTION OF A GRIGNARD REAGENT WITH AN ALLYLIC SUBSTRATE 有权
标题翻译：通过使用具有多种底物的格氏试剂的铜催化偶联反应来生产阿尔法，欧米茄烯的方法
公开(公告)号：US20100280299A1
公开(公告)日：2010-11-04
申请号：US12773458
申请日：2010-05-04
申请人： Krishnamurthy Nacharaju , Paul D. Taylor , Mark J. Cooney , Larry A. Crabtree
发明人： Krishnamurthy Nacharaju , Paul D. Taylor , Mark J. Cooney , Larry A. Crabtree
IPC分类号： C07C11/02
CPC分类号： C07C11/12 , C07C1/326
摘要： A process for the synthesis of linear α,ω-diolefins from an allylic substrate comprises the steps of a) forming the bis-Grignard reagent XMgCH2(CH2)nCH2MgX from an α,ω-acyclic dihalide with X being a halogen; b) preparing a solution comprising an allylic substrate and a copper catalyst; c) catalyzing a coupling reaction by adding to the solution of step (b) the bis-Grignard reagent of step (a); and d) isolating and purifying the α,ω-olefin coupling reaction product.
摘要翻译：从烯丙基底物合成线性α，ω-二烯烃的方法包括以下步骤：a）从具有X为卤素的α，ω-无环二卤化物形成双格氏试剂XMgCH2（CH2）nCH2MgX; b）制备包含烯丙基底物和铜催化剂的溶液; c）通过向步骤（b）的步骤（a）的溶液中加入步骤（a）的双格氏试剂催化偶联反应; 和d）分离和纯化α，ω-烯烃偶联反应产物。

4. 发明申请

US20090068659A1 Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule 有权
标题翻译：用于鉴定核酸分子中一个或多个变体核苷酸序列的方法
公开(公告)号：US20090068659A1
公开(公告)日：2009-03-12
申请号：US12101255
申请日：2008-04-11
申请人： Paul D. Taylor , Reyes Candau , Gary F. Gerard
发明人： Paul D. Taylor , Reyes Candau , Gary F. Gerard
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2525/191 , C12Q2525/186 , C12Q2521/301
摘要： The invention relates to methods for identifying the sequence of one or more variant nucleotides in nucleic acid molecules. The method involves cleaving a double-stranded nucleic acid molecule containing a mismatch with a mismatch-specific endonuclease which cleaves on the 3′ side of the mismatch, and preserving the integrity of the variant nucleotide by ligating a Double-Stranded Linker with a degenerate 3′-overhang to said variant nucleotide. Because the variant nucleotide is immediately adjacent to the linker, PCR and/or sequence-by-synthesis analysis can be readily carried out.
摘要翻译：本发明涉及鉴定核酸分子中一个或多个变体核苷酸序列的方法。该方法包括切割含有错配特异性内切核酸酶的错配的双链核酸分子，其在错配的3'侧切割，并通过连接具有简并3的双链接子来保持变体核苷酸的完整性 '至上述变体核苷酸。因为变体核苷酸与接头直接相邻，所以可以容易地进行PCR和/或逐个序列分析。

5. 发明授权

US06838242B2 Modifying double stranded DNA to enhance separations by matched ion polynucleotide chromatography 失效
标题翻译：通过匹配的离子多核苷酸色谱法修饰双链DNA以增强分离
公开(公告)号：US06838242B2
公开(公告)日：2005-01-04
申请号：US09826055
申请日：2001-04-03
申请人： Douglas T. Gjerde , Paul D. Taylor , Robert M. Haefele
发明人： Douglas T. Gjerde , Paul D. Taylor , Robert M. Haefele
IPC分类号： B01D15/00 , C07H21/02 , C07H21/04 , C12M1/34 , C12P19/34 , C12Q1/68 , G01N33/00
CPC分类号： C12Q1/6816 , C12Q1/6827 , Y10T436/143333 , C12Q2565/137 , C12Q2527/107
摘要： Covalently bound non-polar tags are used to increase the retention times of double stranded polynucleotides on Matched Ion Polynucleotide Chromatography (MIPC) columns. In doing so, separations of DNA mixture components is improved. Additionally, when the non-polar tags are fluorophores, detection limits are also greatly reduced. Strategically tagged primers are used in conduction with PCR to produce DNA fragments having specifically tagged strands. This improves mutation detection by MIPC in several ways. Separations are improved, detection sensitivity is enhanced, and non-stoichiometric addition of wild type DNA prior to hybridization is now possible since only tagged fragments will be observed with a fluorescence detector. Non-polar tags are also used as a novel alternative to G-C clamping during MIPC under partially denaturing conditions. Reversible DNA binding dyes, such as DNA intercalator dyes and DNA groove binding dyes, are used to reduce the detection limit of polynucleotides separated by MIPC.

6. 发明授权

US06503397B2 Non-polar media for polynucleotide separations 有权
公开(公告)号：US06503397B2
公开(公告)日：2003-01-07
申请号：US09828315
申请日：2001-04-05
申请人： Douglas T. Gjerde , Paul D. Taylor
发明人： Douglas T. Gjerde , Paul D. Taylor
IPC分类号： B01D1508
CPC分类号： B01D15/366 , B01J20/28004 , B01J20/28042 , B01J20/287 , B01J20/3204 , B01J20/3225 , B01J20/3227 , B01J20/3268 , C12N15/1006 , C12N15/101
摘要： Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped with a non-polar hydrocarbon or substituted hydrocarbon group. The beads provide efficient separation of polynucleotides using Matched Ion Polynucleotide Chromatography.

7. 发明授权

US06461819B1 Analysis of nicked DNA by matched ion polynucleotide chromatography under denaturing conditions 失效
标题翻译：在变性条件下通过匹配离子多核苷酸色谱分析切口DNA
公开(公告)号：US06461819B1
公开(公告)日：2002-10-08
申请号：US09643120
申请日：2000-08-21
申请人： Douglas T. Gjerde , Paul D. Taylor
发明人： Douglas T. Gjerde , Paul D. Taylor
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q2565/137 , C12Q2565/131
摘要： The present invention provides a method for calibrating a MIPC column wherein the calibration relates to the determination of the organic solvent component in the mobile phase required to elute dsDNA fragments of different base pair lengths at specific retention times. Since a MIPC column affords highly reproducible separations, once calibrated, the base pair length of unknown dsDNA fragments can be determined by comparing their retention times to those obtained on a standard calibration chromatogram. The standard calibration chromatogram is obtained by chromatographing a standard dsDNA ladder containing fragments of known base pair length. In addition, a method is provided to determine the presence of nicks in dsDNA using MIPC under fully denaturing conditions, e.g., 80° C. In one embodiment, this method is applied to the detection of mutations in dsDNA.
摘要翻译：本发明提供了一种用于校准MIPC柱的方法，其中校准涉及在特定保留时间洗脱不同碱基对长度的dsDNA片段所需的流动相中有机溶剂组分的测定。由于MIPC柱提供高度可重复的分离，一旦校准，可以通过将其保留时间与在标准校准色谱图上获得的保留时间进行比较来确定未知dsDNA片段的碱基对长度。标准校准色谱图是通过色谱分离含有已知碱基对长度的片段的标准dsDNA梯度而获得的。此外，提供了一种方法，用于在完全变性条件（例如80℃）下使用MIPC测定dsDNA中缺口的存在。在一个实施方案中，该方法用于检测dsDNA中的突变。

8. 发明授权

US6024878A Method for high resolution liquid chromatographic separation of polynucleotides 有权
标题翻译：多核苷酸高分辨液相色谱分离方法
公开(公告)号：US6024878A
公开(公告)日：2000-02-15
申请号：US183047
申请日：1998-10-30
申请人： Douglas T. Gjerde , Paul D. Taylor , Robert M. Hae Fele
发明人： Douglas T. Gjerde , Paul D. Taylor , Robert M. Hae Fele
IPC分类号： C12N15/09 , B01D15/08 , B01D15/36 , B01J20/26 , B01J20/32 , C12N15/10 , C12Q1/68 , G01N30/34 , G01N30/88
CPC分类号： B01D15/366
摘要： Mixtures of dsDNA fragments are separated by Matched Ion Polynucleotide Chromatography (MIPC) using an isocratic mobile phase to elute polynucleic acid from an MIPC column. The use of isocratic elution conditions provides a marked improvement in the separation of dsDNA fragments compared to gradient elution conditions. Isocratic elution can also be used to effect an improved separation of heteroduplex and homoduplex mixtures when the chromatography is performed under partially denaturing conditions. In addition, dsDNA fragments are bound to the stationary phase under isocratic conditions until a solvent concentration is reached which releases fragments of a particular base pair length range. This separation process is different from the equilibrium partitioning process observed under gradient elution conditions.
摘要翻译：通过使用等度流动相的匹配离子多核苷酸色谱（MIPC）分离dsDNA片段的混合物以从MIPC柱洗脱多核酸。与梯度洗脱条件相比，使用等度洗脱条件提供了dsDNA片段分离的显着改善。当在部分变性条件下进行层析时，等度洗脱也可用于改进异源双链和同源双链混合物的分离。此外，双链DNA片段在等度条件下与固定相结合，直至达到溶剂浓度，释放特定碱基对长度范围的片段。该分离过程不同于在梯度洗脱条件下观察到的平衡分配过程。

9. 发明授权

US5986085A Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments 失效
标题翻译：用于分离多核苷酸片段的匹配离子多核苷酸色谱（MIPC）方法
公开(公告)号：US5986085A
公开(公告)日：1999-11-16
申请号：US65913
申请日：1998-04-24
申请人： Douglas T. Gjerde , Robert M. Haefele , Paul D. Taylor
发明人： Douglas T. Gjerde , Robert M. Haefele , Paul D. Taylor
IPC分类号： C12N15/10 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12N15/101
摘要： A batch process for obtaining polynucleotide fragments, such as dsDNA, having a selected size from a mixture of polynucleotide fragments including the steps of a) applying a solution of the mixture of polynucleotide fragments and a counterion agent to a binding medium having a hydrophobic surface; b) contacting the binding medium with a first stripping solvent and counterion agent, the first stripping solvent having a concentration of organic component sufficient to release from the binding medium all polynucleotide fragments having a size smaller than the selected size, and removing the first stripping solvent from the binding medium; and c) contacting the binding medium with a second stripping solvent having a concentration of organic component sufficient to release from the binding medium the polynucleotide fragments having the selected size, and removing the second stripping solvent from the binding medium. The binding medium can be organic polymer or inorganic particle beads. The mixture of polynucleotides can be the product of a PCR amplification. The binding medium can be contained within a column, a web or a container.
摘要翻译：用于从多核苷酸片段的混合物中获得具有选定大小的多核苷酸片段（例如dsDNA）的分批方法，包括以下步骤：a）将多核苷酸片段和抗衡离子混合物的溶液施用于具有疏水性表面的结合介质; b）使所述结合介质与第一剥离溶剂和抗衡离子剂接触，所述第一剥离溶剂具有足以从所述结合介质释放的有机组分的浓度，所述多个片段的尺寸小于所选择的尺寸，并且除去所述第一剥离溶剂从结合介质; 和c）使所述结合介质与第二剥离溶剂接触，所述第二剥离溶剂具有足以从所述结合介质释放具有所选择尺寸的多核苷酸片段的有机组分浓度，以及从所述结合介质中除去所述第二剥离溶剂。结合介质可以是有机聚合物或无机颗粒珠。多核苷酸的混合物可以是PCR扩增的产物。结合介质可以包含在柱，网或容器中。

10. 发明授权

US5393888A Non-catalytic liquid phase conversion of butyrolactone and ammonia to 2-pyrrolidone product in high yield and selectivity 失效
标题翻译：以高产率和选择性，非催化液相将丁内酯和氨转化为2-吡咯烷酮产物
公开(公告)号：US5393888A
公开(公告)日：1995-02-28
申请号：US204577
申请日：1994-03-02
申请人： Francis B. Minnock , Paul D. Taylor
发明人： Francis B. Minnock , Paul D. Taylor
IPC分类号： C07D207/26 , C07D207/267 , C07D201/08
CPC分类号： C07D207/267
摘要： This invention relates to a liquid phase process for producing 2-pyrrolidone in high yield and selectivity in the absence of catalyst and extraneous solvents which involves contacting ammonia with butyrolactone in a molar ratio of from 0.5:1 to 0.85:1 at high temperature and pressure sufficient to maintain the liquid phase.
摘要翻译：本发明涉及在不存在催化剂和外来溶剂的情况下以高产率和选择性生产2-吡咯烷酮的液相方法，该方法包括使氨与丁酸内酯在摩尔比为0.5:1至0.85:1的高温和高压下接触足以维持液相。

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