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1. 发明申请

US20070243525A1 Method and a device for the evaluation of biopolymer fitness 失效
公开(公告)号：US20070243525A1
公开(公告)日：2007-10-18
申请号：US11730931
申请日：2007-04-04
申请人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehmann
发明人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehmann
IPC分类号： C12Q1/02 , C12Q1/70 , G01J3/30
CPC分类号： G01N21/6452 , C12Q1/04 , C12Q1/6827 , G01N21/64 , G01N21/6402 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G02B21/24 , Y10S435/808 , Y10S436/805 , Y10T436/11 , C12Q2565/107 , C12Q2561/113 , C12Q2535/131
摘要： A Method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined. The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ≧1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 μm from the observation objective.

2. 发明授权

US06582903B1 Method and a device for the evaluation of biopolymer fitness 失效
标题翻译：用于评估生物聚合物适应性的方法和装置
公开(公告)号：US06582903B1
公开(公告)日：2003-06-24
申请号：US09021410
申请日：1998-02-10
申请人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehmann
发明人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehmann
IPC分类号： G01N33543
CPC分类号： G01N21/6402 , G01N21/6428 , G01N21/6458 , G01N2021/6417 , Y10S435/808 , Y10S436/805 , Y10T436/11
摘要： A method for identifying one or a small number of molecules, especially in a dilution of ≦1 &mgr;M, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.
摘要翻译：使用测量时间<= 500ms的激光激发FCS和要分析的分子的短扩散路径来识别一个或少数分子，特别是稀释度为<=1μM的分子的方法，其中测量在通过确定由待分析的分子的发光测量确定的材料特异性参数，优选<=10-14μl的小体积单位。

3. 发明授权

US07534576B2 Method and a device for the evaluation of biopolymer fitness 失效
标题翻译：用于评估生物聚合物适应性的方法和装置
公开(公告)号：US07534576B2
公开(公告)日：2009-05-19
申请号：US11730931
申请日：2007-04-04
申请人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehman
发明人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehman
IPC分类号： G01N33/569
CPC分类号： G01N21/6452 , C12Q1/04 , C12Q1/6827 , G01N21/64 , G01N21/6402 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G02B21/24 , Y10S435/808 , Y10S436/805 , Y10T436/11 , C12Q2565/107 , C12Q2561/113 , C12Q2535/131
摘要： A Method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ≧1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 μm from the observation objective.
摘要翻译：使用测量时间<= 500ms的激光激发FCS和要分析的分子的短扩散路径来识别一个或少数分子，特别是稀释度为<=1μM的分子的方法，其中测量在通过确定由待分析的分子的发光测量确定的材料特异性参数，优选<=10-14μl的小体积单位。可以优选用于执行根据本发明的方法的装置是本身已知的用于在非常稀释的溶液的小测量室中进行荧光激发的激光聚焦的显微镜光学系统，并且用于在后续测量中对发射的光进行成像通过共焦成像，其中使用具有优选> = 1.2NA的高数值孔径的至少一个光学系统，光量受到在显微镜物镜后面的物平面中的共同排列的针孔的限制，并且测量室位于与观察目标之间的距离为0至1000μm。

4. 发明授权

US07241569B2 Method and a device for the evaluation of biopolymer fitness 有权
标题翻译：用于评估生物聚合物适应性的方法和装置
公开(公告)号：US07241569B2
公开(公告)日：2007-07-10
申请号：US10435674
申请日：2003-05-12
申请人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehman
发明人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehman
IPC分类号： G01N33/543
CPC分类号： G01N21/6452 , C12Q1/04 , C12Q1/6827 , G01N21/64 , G01N21/6402 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G02B21/24 , Y10S435/808 , Y10S436/805 , Y10T436/11 , C12Q2565/107 , C12Q2561/113 , C12Q2535/131
摘要： A Method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ≧1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 μm from the observation objective.
摘要翻译：使用测量时间<= 500ms的激光激发FCS和要分析的分子的短扩散路径来识别一个或少数分子，特别是稀释度为<=1μM的分子的方法，其中测量在通过确定通过待检测分子的发光测量确定的材料特异性参数，优选<= 10 -14 / 1的小体积单位。可以优选用于执行根据本发明的方法的装置是本身已知的用于在非常稀释的溶液的小测量室中进行荧光激发的激光聚焦的显微镜光学系统，并且用于在后续测量中对发射的光进行成像通过共焦成像，其中使用具有优选> = 1.2NA的高数值孔径的至少一个光学系统，光量受到在显微镜物镜后面的物平面中的共同排列的针孔的限制，并且测量室位于与观察目标之间的距离为0至1000μm。

5. 发明申请

US20090194706A1 Method and device for the selective withdrawal of compontents from complex mixtures 审中-公开
标题翻译：从复杂混合物中选择性取出组分的方法和装置
公开(公告)号：US20090194706A1
公开(公告)日：2009-08-06
申请号：US12385440
申请日：2009-04-08
申请人： Manfred Eigen , Rudolf Rigler , Karsten Henco
发明人： Manfred Eigen , Rudolf Rigler , Karsten Henco
IPC分类号： G01N21/64 , B67D5/52
CPC分类号： B01L3/0268 , C12M33/07 , G01N15/10 , Y10T436/2575
摘要： The method according to the invention permits a selected withdrawal of one or a few molecularly disperse or cellular components of a system, such as molecules, molecular complexes, vesicles, micelles, cells, optionally together with an associated volume element V having a size of 10−9 l≧V≧10−18 l from a larger sample volume. The selected transfer of the sought component to another environment is effected by defining the space and time of withdrawal by means of a signal correlating with the small component to be withdrawn. The method is particularly useful for the withdrawal of non-abundant components the existence of which can be detected in a preceding step by a scanning process. The method is also useful for the withdrawal of per se unidentified components.
摘要翻译：根据本发明的方法允许选择性地撤出系统的一种或几种分子分散或细胞组分，例如分子，分子复合物，囊泡，胶束，细胞，任选地与尺寸为10的相关体积元素V一起 -9 l> = V> = 10-18 l。所寻求的组件选择的转移到另一环境是通过借助与要撤回的小组件相关联的信号定义撤回的空间和时间来实现的。该方法对于通过扫描过程在前一步骤中可以检测到其存在的非丰富成分是特别有用的。该方法对于本身不确定的组分的撤回也是有用的。

6. 发明申请

US20050250157A1 Method and device for the selective withdrawal of components from complex mixtures 审中-公开
标题翻译：从复杂混合物中选择性取出组分的方法和装置
公开(公告)号：US20050250157A1
公开(公告)日：2005-11-10
申请号：US11015179
申请日：2004-12-20
申请人： Manfred Eigen , Rudolf Rigler , Karsten Henco
发明人： Manfred Eigen , Rudolf Rigler , Karsten Henco
IPC分类号： B01L3/02 , B67D7/70 , C12M1/26 , G01N15/10 , G01N33/53
CPC分类号： B01L3/0268 , C12M33/07 , G01N15/10 , Y10T436/2575
摘要： The method according to the invention permits a selected withdrawal of one or a few molecularly disperse or cellular components of a system, such as molecules, molecular complexes, vesicles, micelles, cells, optionally together with an associated volume element V having a size of 10−9 l≧V≧10−18 l from a larger sample volume. The selected transfer of the sought component to another environment is effected by defining the space and time of withdrawal by means of a signal correlating with the small component to be withdrawn. The method is particularly useful for the withdrawal of non-abundant components the existence of which can be detected in a preceding step by a scanning process. The method is also useful for the withdrawal of per se unidentified components.
摘要翻译：根据本发明的方法允许选择性地撤出系统的一种或几种分子分散或细胞组分，例如分子，分子复合物，囊泡，胶束，细胞，任选地与尺寸为10的相关体积元素V一起从较大的样品体积可以得到。所寻求的组件选择的转移到另一环境是通过借助与要撤回的小组件相关联的信号定义撤回的空间和时间来实现的。该方法对于通过扫描过程在前一步骤中可以检测到其存在的非丰富成分是特别有用的。该方法对于本身不确定的组分的撤回也是有用的。

7. 发明授权

US06849461B2 Method and device for the selective withdrawal of components from complex mixtures 失效
标题翻译：从复杂混合物中选择性取出组分的方法和装置
公开(公告)号：US06849461B2
公开(公告)日：2005-02-01
申请号：US08750715
申请日：1995-06-16
申请人： Manfred Eigen , Rudolf Rigler , Karsten Henco
发明人： Manfred Eigen , Rudolf Rigler , Karsten Henco
IPC分类号： B01L3/02 , B67D7/70 , C12M1/26 , G01N15/10 , G01N21/84 , G01N1/14 , G01N21/64 , G01N21/75 , G01N33/15
CPC分类号： B01L3/0268 , C12M33/07 , G01N15/10 , Y10T436/2575
摘要： The method according to the invention permits a selected withdrawal of one or a few molecularly disperse or cellular components of a system, such as molecules, molecular complexes, vesicles, micelles, cells, optionally together with an associated volume element V having a size of 10−9 l≧V≧10−18 l from a larger sample volume. The selected transfer of the sought component to another environment is effected by defining the space and time of withdrawal by means of a signal correlating with the small component to be withdrawn. The method is particularly useful for the withdrawal of non-abundant components the existence of which can be detected in a preceding step by a scanning process. The method is also useful for the withdrawal of per se unidentified components.
摘要翻译：根据本发明的方法允许选择性地撤出系统的一种或几种分子分散或细胞组分，例如分子，分子复合物，囊泡，胶束，细胞，任选地与尺寸为10的相关体积元素V一起 <9> l> = V> = 10 l。所寻求的组件选择的转移到另一环境是通过借助与要撤回的小组件相关联的信号定义撤回的空间和时间来实现的。该方法对于通过扫描过程在前一步骤中可以检测到其存在的非丰富成分是特别有用的。该方法对于本身不确定的组分的撤回也是有用的。

8. 发明授权

US5807677A Method for direct identification of few nucleic acid strands 失效
标题翻译：用于直接鉴定少量核酸链的方法
公开(公告)号：US5807677A
公开(公告)日：1998-09-15
申请号：US574916
申请日：1995-12-19
申请人： Manfred Eigen , Rudolf Rigler
发明人： Manfred Eigen , Rudolf Rigler
IPC分类号： C12N15/09 , C07H21/04 , C12Q1/68 , C07H19/00 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/6816
摘要： A method for the direct identification of few, preferably single nucleic acid strands of a specific target sequence (10) in a test solution comprising the following steps: a) Preparation of a reference solution with a mixture (11) of different, short primers (12, 13, 14) each with a so-called antisense-sequence (a', b', c') complementary to a section (a, b, c) of the target sequence (10), and marked with one or more dye molecules, b) mixture of the reference solution with the test solution and incubation of this mixture (15) under conditions allowing hybridization of the primers (12, 1, 14) with the nucleic acid strands to be identified, and then c) identification of the target sequence (10) in the incubated solution (15) by discriminating few, preferably one of the nucleic acid strands to be identified to which one or more primers (12, 13, 14) have hybridized against the background of the non-hybridized primers (12, 13, 14).
摘要翻译：一种用于直接鉴定测试溶液中特定靶序列（10）的少数优选单个核酸链的方法，包括以下步骤：a）用不同的短引物（11）的混合物（11）制备参考溶液 12，13,14），每个具有与靶序列（10）的部分（a，b，c）互补的所谓的反义序列（a'，b'，c'），并用一个或多个染料分子，b）参考溶液与测试溶液的混合物，并在允许引物（12,1,14）与要鉴定的核酸链杂交的条件下温育该混合物（15），然后c）鉴定通过区分待鉴定的至少一个或多个引物（12,13,14）与非天然存在的背景杂交的一些或多个引物（12,13,14）杂交的核酸链中的一个，优选地是待孵育溶液（15）中的靶序列（10）杂交引物（12,13,14）。

9. 发明授权

US3972627A Apparatus for investigating fast chemical reactions by optical detection 失效
标题翻译：用于通过光学检测来调查快速化学反应的装置
公开(公告)号：US3972627A
公开(公告)日：1976-08-03
申请号：US487592
申请日：1974-07-11
申请人： Carl-Roland Rabl , Rudolf Rigler , Thomas J. Jovin , Hermann Brundl , Wolfgang Simm
发明人： Carl-Roland Rabl , Rudolf Rigler , Thomas J. Jovin , Hermann Brundl , Wolfgang Simm
IPC分类号： G01N21/03 , G01N21/27 , G01N21/59 , G01N21/64 , G01N21/24
CPC分类号： G01N21/6445 , G01N21/272 , G01N21/645 , G01N2021/0389 , G01N2021/0392 , G01N2021/1738 , G01N2021/6491 , G01N21/0332 , G01N21/59 , G01N2201/1226 , G01N2201/127
摘要： An apparatus for investigating the course of fast chemical reactions, which are initiated in a liquid chemical system under investigation by an external perturbation, e.g. a steep temperature rise (temperature jump). The apparatus comprises first and second light paths conveying a probing light beam and a sense light beam, respectively, and an optical system of extremely high aperture which allows a wide variety of types of measurements, including absorption, fluorescense, fluorescense polarization, and the like with a high signal-to-noise ratio even of small sample concentrations.
摘要翻译：一种用于调查快速化学反应过程的装置，其在通过外部扰动研究的液体化学系统中开始，例如，温度升高（温度上升）。该装置包括分别传送探测光束和感测光束的第一和第二光路以及极高孔径的光学系统，其允许多种类型的测量，包括吸收，荧光，荧光偏振等甚至具有较小的样品浓度的高信噪比。

10. 发明申请

US20120135451A1 Method and Apparatus for Evaluation of Toxicity 审中-公开
标题翻译：用于评估毒性的方法和装置
公开(公告)号：US20120135451A1
公开(公告)日：2012-05-31
申请号：US13376940
申请日：2010-06-08
申请人： Rudolf Rigler
发明人： Rudolf Rigler
IPC分类号： C12Q1/02
CPC分类号： G01N33/5014 , G01N15/1463 , G01N33/5044 , G01N2015/0003
摘要： The present invention pertains to a method for evaluation of the toxicity of compounds comprising the use of spermatozoa. The inventions also relates to a high throughput system for the toxicity evaluation.
摘要翻译：本发明涉及用于评价包含使用精子的化合物的毒性的方法。本发明还涉及用于毒性评估的高通量系统。

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