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1. 发明申请

US20120135451A1 Method and Apparatus for Evaluation of Toxicity 审中-公开
标题翻译：用于评估毒性的方法和装置
公开(公告)号：US20120135451A1
公开(公告)日：2012-05-31
申请号：US13376940
申请日：2010-06-08
申请人： Rudolf Rigler
发明人： Rudolf Rigler
IPC分类号： C12Q1/02
CPC分类号： G01N33/5014 , G01N15/1463 , G01N33/5044 , G01N2015/0003
摘要： The present invention pertains to a method for evaluation of the toxicity of compounds comprising the use of spermatozoa. The inventions also relates to a high throughput system for the toxicity evaluation.
摘要翻译：本发明涉及用于评价包含使用精子的化合物的毒性的方法。本发明还涉及用于毒性评估的高通量系统。

2. 发明授权

US07534576B2 Method and a device for the evaluation of biopolymer fitness 失效
标题翻译：用于评估生物聚合物适应性的方法和装置
公开(公告)号：US07534576B2
公开(公告)日：2009-05-19
申请号：US11730931
申请日：2007-04-04
申请人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehman
发明人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehman
IPC分类号： G01N33/569
CPC分类号： G01N21/6452 , C12Q1/04 , C12Q1/6827 , G01N21/64 , G01N21/6402 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G02B21/24 , Y10S435/808 , Y10S436/805 , Y10T436/11 , C12Q2565/107 , C12Q2561/113 , C12Q2535/131
摘要： A Method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ≧1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 μm from the observation objective.
摘要翻译：使用测量时间<= 500ms的激光激发FCS和要分析的分子的短扩散路径来识别一个或少数分子，特别是稀释度为<=1μM的分子的方法，其中测量在通过确定由待分析的分子的发光测量确定的材料特异性参数，优选<=10-14μl的小体积单位。可以优选用于执行根据本发明的方法的装置是本身已知的用于在非常稀释的溶液的小测量室中进行荧光激发的激光聚焦的显微镜光学系统，并且用于在后续测量中对发射的光进行成像通过共焦成像，其中使用具有优选> = 1.2NA的高数值孔径的至少一个光学系统，光量受到在显微镜物镜后面的物平面中的共同排列的针孔的限制，并且测量室位于与观察目标之间的距离为0至1000μm。

3. 发明申请

US20070248966A1 Parallel High Throughput Single Molecule Sequencing Process 有权
标题翻译：平行高通量单分子测序过程
公开(公告)号：US20070248966A1
公开(公告)日：2007-10-25
申请号：US11628400
申请日：2005-08-05
申请人： Rudolf Rigler
发明人： Rudolf Rigler
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2561/113 , C12Q2521/543 , C12Q2521/319 , C12Q2563/113 , C12Q2563/107 , C12Q2521/519 , C12Q2521/101 , C12Q2565/1025
摘要： The invention relates to a process and to an apparatus for parallel high throughput sequencing of nucleic acid molecules, in particular in the single molecule format. The present invention further relates to a process and an apparatus for a parallel high throughput study of enzyme-substrate interactions, in particular in the single molecule format.
摘要翻译：本发明涉及一种用于核酸分子平行高通量测序的方法和装置，特别是单分子形式。本发明还涉及用于酶 - 底物相互作用的平行高通量研究的方法和装置，特别是单分子形式。

4. 发明授权

US07259847B2 Use of optical diffraction elements in detection methods 有权
$Use of optical diffraction elements in detection methods$
标题翻译：在检测方法中使用光学衍射元件
公开(公告)号：US07259847B2
公开(公告)日：2007-08-21
申请号：US10479177
申请日：2002-05-28
申请人： Rudolf Rigler , Hans Blom , Mathias Johansson , Hard Sverkers
发明人： Rudolf Rigler , Hans Blom , Mathias Johansson , Hard Sverkers
IPC分类号： G01N21/64 , G01J3/30
CPC分类号： G01N21/6452 , G01N21/6458 , G01N2021/6421
摘要： The invention relates to a method for determining luminescent molecules by means of optical excitation in confocal measurement volumes using a diffractive optical element. The method is particularly suitable for single-molecule determination, e.g. by means of fluorescence correlation spectroscopy or by means of dynamic light scattering. An apparatus suitable for carrying out the method according to the invention is furthermore disclosed.
摘要翻译：本发明涉及一种通过使用衍射光学元件的共焦测量体积中的光学激发来测定发光分子的方法。该方法特别适用于单分子测定。通过荧光相关光谱法或通过动态光散射。此外还公开了适用于实施本发明方法的装置。

5. 发明授权

US5116125A Fertility analyzer 失效
标题翻译：生育能力分析仪
公开(公告)号：US5116125A
公开(公告)日：1992-05-26
申请号：US605092
申请日：1990-10-31
申请人： Rudolf Rigler
发明人： Rudolf Rigler
IPC分类号： G01N15/02 , G01P13/00
CPC分类号： G01N15/0205 , G01P13/0086
摘要： A fertility analyzer includes a laser beam impinging on a sperm sample in a capiliary, a first optical system including at least a first lens focusing the laser beam in the sample, and a second optical system including at least a second lens, a first diaphragm determining the volume element of the sample to be monitored, a second diaphragm determining the space angle of scattered light and a light intensity detecter. A signal processing device stores the signals from the light detector during a predetermined time separately for beams outgoing from the sperm sample in at least two chosen directions and computes at least two features having relation to the fertility of the sperm sample. A separator included in the combination of the first and second optical systems sorts out a determinable small section of the beam outgoing from the sperm sample in a direction lying in a plane approximately parallel to a plane including the capillary.
摘要翻译：生殖分析仪包括照射在毛细针上的精子样品的激光束，至少包括将激光束聚焦在样品中的第一透镜的第一光学系统和包括至少第二透镜的第二光学系统，第一光阑确定要监视的样本的体积元素，确定散射光的空间角的第二光阑和光强度检测器。信号处理装置在预定时间内分别存储来自光检测器的信号，用于在至少两个选择的方向上从精子样本出射的束，并且计算与精子样品的生育力有关的至少两个特征。包括在第一和第二光学系统的组合中的分离器在位于大致平行于包括毛细管的平面的平面中的方向上分离出来自精子样品的束的可确定的小部分。

6. 发明授权

US08252604B2 Selection of particles in laminar flow 有权
标题翻译：层流中颗粒的选择
公开(公告)号：US08252604B2
公开(公告)日：2012-08-28
申请号：US11664423
申请日：2005-09-29
申请人： Rudolf Rigler
发明人： Rudolf Rigler
IPC分类号： B03C5/02 , G01N15/14
CPC分类号： G01N15/1459 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0647 , B01L2300/0864 , B01L2400/0415 , G01N15/1404 , G01N15/1484 , G01N30/0005 , G01N2015/149 , Y10T436/118339
摘要： The invention relates to a method for selecting charged particles possessing a predetermined property from a population of a multiplicity of different particles, and also to a device which is suitable for implementing the method.
摘要翻译：本发明涉及从多种不同粒子的群体中选择具有预定特性的带电粒子的方法，还涉及适用于实施该方法的装置。

7. 发明授权

US08017319B2 Evanescence-based multiplex sequencing method 有权
标题翻译：基于Evanescence的多重测序方法
公开(公告)号：US08017319B2
公开(公告)日：2011-09-13
申请号：US10499547
申请日：2002-12-18
申请人： Rudolf Rigler
发明人： Rudolf Rigler
IPC分类号： C12Q1/68 , C12M1/00 , C12M1/34 , C12M3/00 , G02B6/10 , C07H21/02
CPC分类号： C12Q1/6869 , C12Q2565/501 , C12Q2565/518 , C12Q2521/319 , C12Q2563/107
摘要： The invention relates to a method and a device for evanescence-based multiplex sequencing of nucleic acid molecules immobilized on a support.
摘要翻译：本发明涉及一种用于固定在载体上的核酸分子的基于消逝的多重测序的方法和装置。

8. 发明授权

US07553620B2 Method for determining polynucleotides in a sample without attaching these to a support, and using detection probes 失效
标题翻译：用于确定样品中多核苷酸的方法，而不将其连接到载体上，并使用检测探针
公开(公告)号：US07553620B2
公开(公告)日：2009-06-30
申请号：US10276039
申请日：2001-05-11
申请人： Rudolf Rigler , Zeno Foldes-Papp
发明人： Rudolf Rigler , Zeno Foldes-Papp
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816
摘要： The invention relates to a method for detecting polynucleotides on a substrate, whereby the substrate contains a multitude of separate detection regions each accommodating different polynucleotides. The polynucleotides in the detection regions exist, contrary to known DNA chip test formats, in a form that is not bound to the substrate. The invention also relates to a device for implementing said method.
摘要翻译：本发明涉及一种用于检测底物上的多核苷酸的方法，由此所述底物含有多个单独的检测区域，每个检测区域各自容纳不同的多核苷酸。与已知的DNA芯片测试形式相反，检测区域中的多核苷酸以不与底物结合的形式存在。本发明还涉及一种用于实现所述方法的装置。

9. 发明申请

US20070243525A1 Method and a device for the evaluation of biopolymer fitness 失效
公开(公告)号：US20070243525A1
公开(公告)日：2007-10-18
申请号：US11730931
申请日：2007-04-04
申请人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehmann
发明人： Rudolf Rigler , Manfred Eigen , Karsten Henco , Ulo Mets , Jerker Widengren , Michael Stuke , Michael Brinkmeyer , Wolfgang Simm , Olaf Lehmann
IPC分类号： C12Q1/02 , C12Q1/70 , G01J3/30
CPC分类号： G01N21/6452 , C12Q1/04 , C12Q1/6827 , G01N21/64 , G01N21/6402 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G02B21/24 , Y10S435/808 , Y10S436/805 , Y10T436/11 , C12Q2565/107 , C12Q2561/113 , C12Q2535/131
摘要： A Method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined. The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ≧1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 μm from the observation objective.

10. 发明授权

US07227631B2 Method for examining a test sample by means of fluorescence spectroscopy, especially fluorescence correlatin spectroscopy, and device for carrying out said method 失效
标题翻译：通过荧光光谱法，特别是荧光相关光谱法检测测试样品的方法，以及用于实施所述方法的装置
公开(公告)号：US07227631B2
公开(公告)日：2007-06-05
申请号：US10470246
申请日：2002-01-25
申请人： Lars Edman , Rudolf Rigler
发明人： Lars Edman , Rudolf Rigler
IPC分类号： G01N21/01
CPC分类号： G01N21/6408 , G01N1/40 , G01N21/03 , G01N21/6452 , G01N2001/4038
摘要： In a method for the fluorescence spectroscopic, in particular fluorescence correlation spectroscopic study of a measurement sample, the latter is introduced into a sample holding chamber (12) formed hollowed in a sample support (10). Electrically charged analytes contained in the measurement sample are then concentrated in a measurement volume (26) lying inside the sample volume, whereupon the measurement volume (26) is studied. According to the invention, the boundary walls (28) of the sample holding chamber (12) are brought essentially surface-wide to an electrostatic potential having the same sign as the charge of the analytes in order to concentrate the analytes in the measurement volume (26). An electrical molecule trap formed in this way can be produced very straightforwardly and is suitable in particular for screening studies of measurement samples, a large multiplicity of which are arranged close together in indentations (12) of the sample support (10).
摘要翻译：在用于荧光光谱的方法中，特别是测量样品的荧光相关光谱学研究，将后者引入样品支持体（10）中形成的样品保持室（12）中。然后将包含在测量样品中的电荷分析物浓缩在位于样品体积内的测量体积（26）中，由此研究测量体积（26）。根据本发明，样品保持室（12）的边界壁（28）基本上在表面上具有与分析物的电荷相同符号的静电势，以便将分析物浓缩到测量体积（ 26）。以这种方式形成的电分子陷阱可以非常直接地产生，并且特别适用于筛选研究样品的测量样品，其中大量的测量样品在样品支撑体（10）的凹陷（12）中靠近放置在一起。

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