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1. 发明授权

US07393525B2 Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same 失效
标题翻译：在动物细胞中引入和表达基因的方法以及用于其中的活的侵入性细菌载体
公开(公告)号：US07393525B2
公开(公告)日：2008-07-01
申请号：US10369519
申请日：2003-02-21
申请人： Robert J. Powell , George K. Lewis , David M. Hone
发明人： Robert J. Powell , George K. Lewis , David M. Hone
IPC分类号： A01N63/00 , A61K48/00 , A61K39/00 , C12N15/63
CPC分类号： A61K48/0008 , A61K48/00 , A61K48/0091 , A61K2039/51 , A61K2039/523 , A61K2039/5254 , A61K2039/53 , C12N15/87
摘要： A method for introducing and expressing genes in animal cells is disclosed comprising infecting said the animal cells with live invasive bacteria, wherein bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.
摘要翻译：公开了用于在动物细胞中引入和表达基因的方法，其包括用活细菌感染所述动物细胞，其中细菌含有编码所述基因的真核表达盒。该基因可以编码例如疫苗抗原，治疗剂，免疫调节剂或反义RNA或催化RNA。

2. 发明授权

US06548287B1 Non-pyrogenic bacterial strains and use of the same 失效
标题翻译：非热原菌菌株和使用相同
公开(公告)号：US06548287B1
公开(公告)日：2003-04-15
申请号：US09339180
申请日：1999-06-24
申请人： Robert J. Powell , David M. Hone
发明人： Robert J. Powell , David M. Hone
IPC分类号： A61K3910
CPC分类号： C12R1/01 , A61K48/00 , C12N15/74 , C12P19/04
摘要： The present invention provides gram-negative bacterial strains that produce substantially pure non-pyrogenic lipopolysaccharide or lipid A. The present invention also relates to a use of said strains for the preparation of non-pyrogenic DNA and use of the same for introducing endogenous or foreign genes into animal cells or animal tissue. Further, the present invention relates to a use of said strains for the preparation of non-pyrogenic recombinant mammalian, protozoan and viral proteins. Furthermore, the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial vaccines and vaccine vectors. Yet a further use of the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial proteins and polysaccharides antigens for use as vaccines.
摘要翻译：本发明提供了产生基本上纯的非致热性脂多糖或脂质A的革兰氏阴性细菌菌株。本发明还涉及所述菌株用于制备非致热原DNA的用途，并且使用该菌株用于引入内源或外源基因进入动物细胞或动物组织。此外，本发明涉及所述菌株用于制备非致热性重组哺乳动物，原生动物和病毒蛋白质的用途。此外，本发明涉及所述菌株用于制备非致热性细菌疫苗和疫苗载体的用途。本发明的进一步应用涉及所述菌株用于制备用作疫苗的非致热性细菌蛋白质和多糖抗原的用途。

3. 发明授权

US5877159A Method for introducing and expressing genes in animal cells and live invasive bacterial vectors for use in the same 失效
标题翻译：在动物细胞中引入和表达基因和活体侵袭性细菌载体用于其中的方法
公开(公告)号：US5877159A
公开(公告)日：1999-03-02
申请号：US433790
申请日：1995-05-03
申请人： Robert J. Powell , George K. Lewis , David M. Hone
发明人： Robert J. Powell , George K. Lewis , David M. Hone
IPC分类号： C12N15/09 , A61K35/74 , A61K39/00 , A61K45/00 , A61K48/00 , A61P31/04 , A61P31/12 , A61P33/00 , C12N1/21 , C12N5/10 , C12N15/87 , A01N43/04 , A61K31/70 , C12N15/63
CPC分类号： C12N15/87 , A61K48/00 , A61K2039/51
摘要： A method for introducing and expressing genes in animal cells is disclosed comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.
摘要翻译：公开了用于在动物细胞中引入和表达基因的方法，其包括用活细菌感染所述动物细胞，其中所述细菌含有编码所述基因的真核表达盒。该基因可以编码例如疫苗抗原，治疗剂，免疫调节剂或反义RNA或催化RNA。

4. 发明授权

US6150170A Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same 失效
公开(公告)号：US6150170A
公开(公告)日：2000-11-21
申请号：US126220
申请日：1998-07-30
申请人： Robert J. Powell , George K. Lewis , David M. Hone
发明人： Robert J. Powell , George K. Lewis , David M. Hone
IPC分类号： A61K48/00 , C12N15/87 , C12N15/63 , A01N43/04 , A61K31/70 , C12N5/00 , C12N15/00
CPC分类号： A61K48/00 , C12N15/87 , A61K2039/523 , A61K2039/5254 , A61K2039/53
摘要： A method for introducing and expressing genes in animal cells is disclosed comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.

5. 发明授权

US5997881A Method of making non-pyrogenic lipopolysaccharide or A 失效
标题翻译：制备非致热性脂多糖或脂质A的方法
公开(公告)号：US5997881A
公开(公告)日：1999-12-07
申请号：US802371
申请日：1997-02-19
申请人： Robert J. Powell , David M. Hone
发明人： Robert J. Powell , David M. Hone
IPC分类号： A61K48/00 , A61P31/04 , C12N15/74 , C12P19/04 , A61K39/02
CPC分类号： C12R1/01 , A61K48/00 , C12N15/74 , C12P19/04
摘要： The present invention provides gram-negative bacterial strains that produce substantially pure non-pyrogenic lipopolysaccharide or lipid A. The present invention also relates to a use of said strains for the preparation of non-pyrogenic DNA and use of the same for introducing endogenous or foreign genes into animal cells or animal tissue. Further, the present invention relates to a use of said strains for the preparation of non-pyrogenic recombinant mammalian, protozoan and viral proteins. Furthermore, the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial vaccines and vaccine vectors. Yet a further use of the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial proteins and polysaccharide antigens for use as vaccines.
摘要翻译：本发明提供了产生基本上纯的非致热性脂多糖或脂质A的革兰氏阴性细菌菌株。本发明还涉及所述菌株用于制备非致热原DNA的用途，并且使用该菌株用于引入内源或外源基因进入动物细胞或动物组织。此外，本发明涉及所述菌株用于制备非致热性重组哺乳动物，原生动物和病毒蛋白质的用途。此外，本发明涉及所述菌株用于制备非致热性细菌疫苗和疫苗载体的用途。本发明的另一个用途涉及所述菌株用于制备用作疫苗的非致热性细菌蛋白质和多糖抗原的用途。

6. 发明授权

US06682729B1 Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same 失效
标题翻译：在动物细胞中引入和表达基因的方法以及用于其中的活的侵入性细菌载体
公开(公告)号：US06682729B1
公开(公告)日：2004-01-27
申请号：US09420770
申请日：1999-10-19
申请人： Robert J. Powell , George K. Lewis , David M. Hone
发明人： Robert J. Powell , George K. Lewis , David M. Hone
IPC分类号： A01N6300
CPC分类号： A61K48/0008 , A61K48/00 , A61K48/0091 , A61K2039/51 , A61K2039/523 , A61K2039/5254 , A61K2039/53 , C12N15/87
摘要： A method for introducing and expressing genes in animal cells is disclosed comprising infecting the animal cells with live invasive bacteria, wherein the bacteria contain a eukaryotic expression cassette encoding the gene. The gene may encode, e.g., a vaccine antigen, a therapeutic agent, an immunoregulatory agent or an anti-sense RNA or a catalytic RNA.
摘要翻译：公开了用于在动物细胞中引入和表达基因的方法，其包括用活的侵入性细菌感染动物细胞，其中所述细菌含有编码该基因的真核表达盒。该基因可编码例如疫苗抗原，治疗剂，免疫调节剂或反义RNA或催化RNA。

7. 发明授权

US09039996B2 Silicon substrate optimization for microarray technology 有权
标题翻译：微阵列技术的硅衬底优化
公开(公告)号：US09039996B2
公开(公告)日：2015-05-26
申请号：US13272063
申请日：2011-10-12
申请人： Massimiliano Pesaturo , Robert J. Powell
发明人： Massimiliano Pesaturo , Robert J. Powell
IPC分类号： B01L3/00 , C40B60/10
CPC分类号： B01L3/502707 , B01J2219/00317 , B01J2219/00466 , B01J2219/00702 , B01L3/502753 , B01L3/502761 , B01L2200/0668 , B01L2300/16 , B01L2300/161 , C40B60/10 , G01B5/00 , Y10T428/24355
摘要： A micro device includes a substrate and a structure configured to bind to an object or a material, or not to bind to an object or material. The structure has a roughness based on a roughness of the object or material. For example, a microarray includes a substrate and a well positioned in the substrate and configured to bind to a type of bead. The well has a roughness based on a roughness of the type of bead to which the well is configured to bind. The roughness of the well is controlled by controlling a position and number of striations in the side of the well. In another example, a moveable component of a micro device may have a roughness different from a roughness of an adjacent component, to reduce the likelihood of the moveable component sticking to the adjacent component.
摘要翻译：微型装置包括衬底和构造成结合物体或材料的结构，或不结合物体或材料。该结构具有基于物体或材料的粗糙度的粗糙度。例如，微阵列包括基底和定位在基底中并且被配置为结合一种珠粒的孔。井具有基于井的类型的粗糙度的孔的粗糙度，孔被构造成结合。井的粗糙度通过控制井的位置和条纹的数量来控制。在另一示例中，微型装置的可移动部件可以具有与相邻部件的粗糙度不同的粗糙度，以减少可移动部件粘附到相邻部件的可能性。

8. 发明授权

US5198729A CRT monitor with elimination of unwanted time variable electric field 失效
标题翻译： CRT显示器消除无用的时间可变电场
公开(公告)号：US5198729A
公开(公告)日：1993-03-30
申请号：US907686
申请日：1992-07-02
申请人： Robert J. Powell
发明人： Robert J. Powell
IPC分类号： G09G1/00 , H04N3/185
CPC分类号： G09G1/00 , H04N3/185
摘要： Unwanted electric fields in the area in front of a CRT can be produced by flyback voltage pulses which occur across the horizontal deflection coils and are coupled to the inner DAG coating of the CRT. The unwanted fields can be eliminated by providing voltage pulses equal and opposite to the causative voltage pulses and applying the provided voltage pulses to the inner coating and the anode button, leaving no fields to be cancelled in the relevant area.
摘要翻译：可以通过在水平偏转线圈两端发生的回扫电压脉冲来产生CRT前方区域中的不需要的电场，并且与CRT的内部DAG涂层耦合。可以通过提供与原因电压脉冲相等和相反的电压脉冲来消除不需要的场，并将所提供的电压脉冲施加到内部涂层和阳极按钮，在相关区域中不会取消场。

9. 发明申请

US20120093700A1 SILICON SUBSTRATE OPTIMIZATION FOR MICROARRAY TECHNOLOGY 有权
标题翻译：硅基板优化微波技术
公开(公告)号：US20120093700A1
公开(公告)日：2012-04-19
申请号：US13272063
申请日：2011-10-12
申请人： Massimiliano Pesaturo , Robert J. Powell
发明人： Massimiliano Pesaturo , Robert J. Powell
IPC分类号： G06F19/00 , B01J19/00
CPC分类号： B01L3/502707 , B01J2219/00317 , B01J2219/00466 , B01J2219/00702 , B01L3/502753 , B01L3/502761 , B01L2200/0668 , B01L2300/16 , B01L2300/161 , C40B60/10 , G01B5/00 , Y10T428/24355
摘要： A micro device includes a substrate and a structure configured to bind to an object or a material, or not to bind to an object or material. The structure has a roughness based on a roughness of the object or material. For example, a microarray includes a substrate and a well positioned in the substrate and configured to bind to a type of bead. The well has a roughness based on a roughness of the type of bead to which the well is configured to bind. The roughness of the well is controlled by controlling a position and number of striations in the side of the well. In another example, a moveable component of a micro device may have a roughness different from a roughness of an adjacent component, to reduce the likelihood of the moveable component sticking to the adjacent component.
摘要翻译：微型装置包括衬底和构造成结合物体或材料的结构，或不结合物体或材料。该结构具有基于物体或材料的粗糙度的粗糙度。例如，微阵列包括基底和定位在基底中并且被配置为结合一种珠粒的孔。井具有基于井的类型的粗糙度的孔的粗糙度，孔被构造成结合。井的粗糙度通过控制井的位置和条纹的数量来控制。在另一示例中，微型装置的可移动部件可以具有与相邻部件的粗糙度不同的粗糙度，以减少可移动部件粘附到相邻部件的可能性。

10. 发明授权

US07125718B2 Method for introducing and expressing genes in animal cells, and bacterial blebs for use in same 失效
标题翻译：在动物细胞中引入和表达基因的方法，以及用于其中的细菌泡
公开(公告)号：US07125718B2
公开(公告)日：2006-10-24
申请号：US10432149
申请日：2001-05-24
申请人： Robert J. Powell , David Hone
发明人： Robert J. Powell , David Hone
IPC分类号： C12N15/88 , C12N15/85 , C12P21/06 , A61K9/127
CPC分类号： C12N15/88 , A61K39/025 , A61K39/0275 , A61K39/0283 , A61K48/00 , A61K2039/523 , A61K2039/53 , A61K2039/542 , A61K2039/552 , Y02A50/476 , Y02A50/482
摘要： A method for introducing and expressing genes in animal cells, in which the animal cells are transfected with bacterial blebs containing a eukaryotic expression cassette encoding the gene. Bacterial blebs comprising a eukaryotic expression cassette, wherein the bacterial blebs are derived from gram negative bacteria.
摘要翻译：一种用于在动物细胞中引入和表达基因的方法，其中用含有编码该基因的真核表达盒的细菌细胞泡转染动物细胞。包含真核表达盒的细菌泡，其中细菌泡从革兰氏阴性细菌衍生。

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