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1. 发明授权

US06500419B1 Method for introducing and expressing RNA in animal cells 失效
标题翻译：在动物细胞中引入和表达RNA的方法
公开(公告)号：US06500419B1
公开(公告)日：2002-12-31
申请号：US09545153
申请日：2000-04-07
申请人： David M. Hone , George Lewis , Robert Powell
发明人： David M. Hone , George Lewis , Robert Powell
IPC分类号： A61K4800
CPC分类号： C07K14/005 , A61K48/00 , C07K14/24 , C12N15/70 , C12N15/74 , C12N15/85 , C12N15/87 , C12N2740/16122 , C12N2830/002 , C12N2830/50 , C12N2830/55 , C12N2840/203 , C12N2840/206 , C12R1/01
摘要： The invention pertains to a method for introducing RNA molecules into eukaryotic cells, wherein the RNA molecules are capable of being translated in the eukaryotic cells or is an antisense RNA or a catalytic RNA, as well as to such bacteria, compositions comprising such bacteria, and nucleic acids which can be introduced into bacteria for practicing the method of the invention. Examples of products the RNA molecule may encode include vaccine antigens, therapeutic agents, immunoregulatory agents or anti-sense RNA molecules or catalytic RNA molecules.
摘要翻译：本发明涉及将RNA分子引入真核细胞的方法，其中RNA分子能够在真核细胞中翻译，或者是反义RNA或催化RNA，以及这些细菌，包含这种细菌的组合物，和可以引入细菌以实施本发明方法的核酸。 RNA分子可编码的产物的实例包括疫苗抗原，治疗剂，免疫调节剂或反义RNA分子或催化RNA分子。

2. 发明授权

US6150170A Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same 失效
公开(公告)号：US6150170A
公开(公告)日：2000-11-21
申请号：US126220
申请日：1998-07-30
申请人： Robert J. Powell , George K. Lewis , David M. Hone
发明人： Robert J. Powell , George K. Lewis , David M. Hone
IPC分类号： A61K48/00 , C12N15/87 , C12N15/63 , A01N43/04 , A61K31/70 , C12N5/00 , C12N15/00
CPC分类号： A61K48/00 , C12N15/87 , A61K2039/523 , A61K2039/5254 , A61K2039/53
摘要： A method for introducing and expressing genes in animal cells is disclosed comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.

3. 发明授权

US5997881A Method of making non-pyrogenic lipopolysaccharide or A 失效
标题翻译：制备非致热性脂多糖或脂质A的方法
公开(公告)号：US5997881A
公开(公告)日：1999-12-07
申请号：US802371
申请日：1997-02-19
申请人： Robert J. Powell , David M. Hone
发明人： Robert J. Powell , David M. Hone
IPC分类号： A61K48/00 , A61P31/04 , C12N15/74 , C12P19/04 , A61K39/02
CPC分类号： C12R1/01 , A61K48/00 , C12N15/74 , C12P19/04
摘要： The present invention provides gram-negative bacterial strains that produce substantially pure non-pyrogenic lipopolysaccharide or lipid A. The present invention also relates to a use of said strains for the preparation of non-pyrogenic DNA and use of the same for introducing endogenous or foreign genes into animal cells or animal tissue. Further, the present invention relates to a use of said strains for the preparation of non-pyrogenic recombinant mammalian, protozoan and viral proteins. Furthermore, the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial vaccines and vaccine vectors. Yet a further use of the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial proteins and polysaccharide antigens for use as vaccines.
摘要翻译：本发明提供了产生基本上纯的非致热性脂多糖或脂质A的革兰氏阴性细菌菌株。本发明还涉及所述菌株用于制备非致热原DNA的用途，并且使用该菌株用于引入内源或外源基因进入动物细胞或动物组织。此外，本发明涉及所述菌株用于制备非致热性重组哺乳动物，原生动物和病毒蛋白质的用途。此外，本发明涉及所述菌株用于制备非致热性细菌疫苗和疫苗载体的用途。本发明的另一个用途涉及所述菌株用于制备用作疫苗的非致热性细菌蛋白质和多糖抗原的用途。

4. 发明授权

US07393525B2 Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same 失效
标题翻译：在动物细胞中引入和表达基因的方法以及用于其中的活的侵入性细菌载体
公开(公告)号：US07393525B2
公开(公告)日：2008-07-01
申请号：US10369519
申请日：2003-02-21
申请人： Robert J. Powell , George K. Lewis , David M. Hone
发明人： Robert J. Powell , George K. Lewis , David M. Hone
IPC分类号： A01N63/00 , A61K48/00 , A61K39/00 , C12N15/63
CPC分类号： A61K48/0008 , A61K48/00 , A61K48/0091 , A61K2039/51 , A61K2039/523 , A61K2039/5254 , A61K2039/53 , C12N15/87
摘要： A method for introducing and expressing genes in animal cells is disclosed comprising infecting said the animal cells with live invasive bacteria, wherein bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.
摘要翻译：公开了用于在动物细胞中引入和表达基因的方法，其包括用活细菌感染所述动物细胞，其中细菌含有编码所述基因的真核表达盒。该基因可以编码例如疫苗抗原，治疗剂，免疫调节剂或反义RNA或催化RNA。

5. 发明授权

US06596477B1 Treatment and prevention of immunodeficiency virus infection by administration of non-pyrogenic derivatives of lipid A 失效
标题翻译：通过施用脂质A的非致热原衍生物来治疗和预防免疫缺陷病毒感染
公开(公告)号：US06596477B1
公开(公告)日：2003-07-22
申请号：US09536668
申请日：2000-03-27
申请人： David M. Hone , Richard Crowley , George Lewis
发明人： David M. Hone , Richard Crowley , George Lewis
IPC分类号： C12Q170
CPC分类号： A61K31/715 , A61K38/195 , A61K45/06 , Y02A50/473 , A61K2300/00
摘要： The present inventors have found that certain preparations containing LPS and/or lipid A variants, derivatives, and/or analogs demonstrate non-pyrogenic properties and exhibit anti-viral activities. In particular, non-pyrogenic preparations of LPS, lipid A, LPS antagonists and lipid A antagonists, and derivatives thereof induce &bgr; chemokine secretion, such as MIP-1&bgr;, but not proinflammatory cytokines, such as TNF&agr;, IL-1&bgr; and IL-6. Non-pyrogenic preparations of the invention have been demonstrated by the Applicant to suppress HIV replication in human peripheral blood monocytes, as described by way of example herein. The present invention provides preparations of LPS or lipid A variants, analogs and derivatives of decreased or absent pyrogenicity which can be used as therapeutics for the treatment or prevention of immunodeficiency virus infection and its consequences.
摘要翻译：本发明人已经发现含有LPS和/或脂质A变体，衍生物和/或类似物的某些制剂表现出非热原性质并表现出抗病毒活性。特别地，LPS，脂质A，LPS拮抗剂和脂质A拮抗剂及其衍生物的非致热制剂诱导β趋化因子分泌，例如MIP-1β，但不引起促炎细胞因子，例如TNFα，IL-1β和IL-6 。本发明的非热原制剂已经被申请人证实，以抑制人外周血单核细胞中的HIV复制，如本文实施例所述。本发明提供可用作治疗或预防免疫缺陷病毒感染的治疗剂及其后果的LPS或脂质A变体，类似物和降低或不存在致热原性的衍生物的制剂。

6. 发明授权

US06548287B1 Non-pyrogenic bacterial strains and use of the same 失效
标题翻译：非热原菌菌株和使用相同
公开(公告)号：US06548287B1
公开(公告)日：2003-04-15
申请号：US09339180
申请日：1999-06-24
申请人： Robert J. Powell , David M. Hone
发明人： Robert J. Powell , David M. Hone
IPC分类号： A61K3910
CPC分类号： C12R1/01 , A61K48/00 , C12N15/74 , C12P19/04
摘要： The present invention provides gram-negative bacterial strains that produce substantially pure non-pyrogenic lipopolysaccharide or lipid A. The present invention also relates to a use of said strains for the preparation of non-pyrogenic DNA and use of the same for introducing endogenous or foreign genes into animal cells or animal tissue. Further, the present invention relates to a use of said strains for the preparation of non-pyrogenic recombinant mammalian, protozoan and viral proteins. Furthermore, the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial vaccines and vaccine vectors. Yet a further use of the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial proteins and polysaccharides antigens for use as vaccines.
摘要翻译：本发明提供了产生基本上纯的非致热性脂多糖或脂质A的革兰氏阴性细菌菌株。本发明还涉及所述菌株用于制备非致热原DNA的用途，并且使用该菌株用于引入内源或外源基因进入动物细胞或动物组织。此外，本发明涉及所述菌株用于制备非致热性重组哺乳动物，原生动物和病毒蛋白质的用途。此外，本发明涉及所述菌株用于制备非致热性细菌疫苗和疫苗载体的用途。本发明的进一步应用涉及所述菌株用于制备用作疫苗的非致热性细菌蛋白质和多糖抗原的用途。

7. 发明申请

US20100047286A1 Recombinant BCG strains with enhanced ability to escape the endosome 审中-公开
标题翻译：具有增强脱逃内体能力的重组BCG菌株
公开(公告)号：US20100047286A1
公开(公告)日：2010-02-25
申请号：US11578124
申请日：2005-11-23
申请人： Ronggai Sun , David M. Hone , Jerald C. Sadoff
发明人： Ronggai Sun , David M. Hone , Jerald C. Sadoff
IPC分类号： A61K39/04 , C12N1/21 , A61P31/04
CPC分类号： A61K39/04 , A61K2039/522 , A61K2039/523 , C07K14/33 , Y02A50/412
摘要： Mycobacterium strains that have an enhanced ability to elicit a Histocompatibility-Class I-restricted CD8+ T cell immune response are provided. The Mycobacterium strains are genetically engineered to express a endosomalytic protein that is active at neutral pH (e.g. Perfringolysin O), permitting escape of the Mycobacterium from endosomes into the cytoplasm of the cell. The invention also provides vaccine preparations containing the Mycobacterium strains.
摘要翻译：提供了具有增强的能够引发组织相容性I类限制性CD8 + T细胞免疫应答的分枝杆菌菌株。分枝杆菌菌株进行遗传工程改造以表达在中性pH（例如Perfringolysin O）上有活性的内分泌蛋白，允许分枝杆菌从内体逃逸到细胞的细胞质中。本发明还提供含有分枝杆菌菌株的疫苗制剂。

8. 发明授权

US06841345B1 Treatment and prevention of immunodeficiency virus infection by administration of non-pyrogenic derivatives of lipid A 失效
标题翻译：通过施用脂质A的非致热原衍生物来治疗和预防免疫缺陷病毒感染
公开(公告)号：US06841345B1
公开(公告)日：2005-01-11
申请号：US09383709
申请日：1999-08-26
申请人： David M. Hone , Richard Crowley , George Lewis
发明人： David M. Hone , Richard Crowley , George Lewis
IPC分类号： A61K39/02 , A61K45/06 , C12Q1/70
CPC分类号： A61K31/7024 , A61K39/02 , A61K45/06 , Y02A50/478
摘要： The present inventors have found that certain preparations containing LPS and/or lipid A variants, derivatives, and/or analogs demonstrate non-pyrogenic properties and exhibit anti-viral activities. In particular, non-pyrogenic preparations of LPS, lipid A, LPS antagonists and lipid A antagonists, and derivatives thereof induce β chemokine secretion, such as MIP-1β, but not proinflammatory cytokines, such as TNFα, IL-1β and IL-6. Non-pyrogenic preparations of the invention have been demonstrated by the Applicant to suppress HIV replication in human peripheral blood monocytes, as described by way of example herein. The present invention provides preparations of LPS or lipid A variants, analogs and derivatives of decreased or absent pyrogenicity which can be used as therapeutics for the treatment or prevention of immunodeficiency virus infection and its consequences.
摘要翻译：本发明人已经发现含有LPS和/或脂质A变体，衍生物和/或类似物的某些制剂表现出非热原性质并表现出抗病毒活性。特别地，LPS，脂质A，LPS拮抗剂和脂质A拮抗剂及其衍生物的非致热制剂诱导β趋化因子分泌，例如MIP-1β，但不引起促炎细胞因子，例如TNFα，IL-1β和IL-6 。本发明的非热原制剂已经被申请人证实，以抑制人外周血单核细胞中的HIV复制，如本文实施例所述。本发明提供可用作治疗或预防免疫缺陷病毒感染的治疗剂及其后果的LPS或脂质A变体，类似物和降低或不存在致热原性的衍生物的制剂。

9. 发明授权

US06759241B1 Adjuvant comprising a lipopolysaccharide antagonist 失效
标题翻译：佐剂包含脂多糖拮抗剂
公开(公告)号：US06759241B1
公开(公告)日：2004-07-06
申请号：US10110072
申请日：2003-02-27
申请人： David M. Hone , Richard Crowley , Mohamed Tarek Shata
发明人： David M. Hone , Richard Crowley , Mohamed Tarek Shata
IPC分类号： C12N502
CPC分类号： C12P19/44 , A61K39/39 , A61K2039/55572 , Y02A50/403 , Y02A50/41 , Y02A50/412 , Y02A50/466 , Y10S435/81
摘要： LPS preparations, isolated from gram negative bacterial strains that contain at least one mutation in at least one of the htrB and msbB genes, and methods and therapeutics related thereto. The LPS preparations display both LPS antagonist and adjuvant activities.
摘要翻译：从在htrB和msbB基因中的至少一个中含有至少一个突变的革兰氏阴性细菌菌株分离的LPS制剂，以及与其相关的方法和治疗方法。 LPS制剂显示LPS拮抗剂和佐剂活性。

10. 发明授权

US06368604B1 Non-pyrogenic derivatives of lipid A 失效
标题翻译：脂质A的非致热原衍生物
公开(公告)号：US06368604B1
公开(公告)日：2002-04-09
申请号：US08938106
申请日：1997-09-26
申请人： David M. Hone , Richard Crowley , George Lewis
发明人： David M. Hone , Richard Crowley , George Lewis
IPC分类号： A61K3902
CPC分类号： A61K31/7024 , A61K39/02 , A61K45/06 , Y02A50/478
摘要： The present inventors have found that certain preparations containing LPS and/or lipid A variants, derivatives, and/or analogs demonstrate non-pyrogenic properties and exhibit anti-viral activities. In particular, non-pyrogenic preparations of LPS, lipid A, LPS antagonists and lipid A antagonists, and derivatives thereof induce &bgr; chemokine secretion, such as MIP-1&bgr;, but not proinflammatory cytokines, such as TNF&agr;, IL-1&bgr; and IL-6. Non-pyrogenic preparations of the invention have been demonstrated by the Applicant to suppress HIV replication in human peripheral blood monocytes, as described by way of example herein. The present invention provides preparations of LPS or lipid A variants, analogs and derivatives of decreased or absent pyrogenicity which can be used as therapeutics for the treatment or prevention of immunodeficiency virus infection and its consequences.
摘要翻译：本发明人已经发现，含有LPS和/或脂质A变体，衍生物和/或类似物的某些制剂表现出非热原性质并表现出抗病毒活性。特别地，LPS，脂质A，LPS拮抗剂和脂质A拮抗剂及其衍生物的非致热制剂诱导β趋化因子分泌，例如MIP-1β，但不引起促炎细胞因子，例如TNFα，IL-1β和IL-6 。本发明的非热原制剂已经被申请人证实，以抑制人外周血单核细胞中的HIV复制，如本文实施例所述。本发明提供可用作治疗或预防免疫缺陷病毒感染的治疗剂及其后果的LPS或脂质A变体，类似物和降低或不存在致热原性的衍生物的制剂。

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