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    • 1. 发明申请
    • Screening Method for Specific Protein in Proteome Comprehensive Analysis
    • 蛋白质组学综合分析中特异性蛋白质的筛选方法
    • US20090132171A1
    • 2009-05-21
    • US11915981
    • 2006-02-08
    • Rieko GotoShohei ShioyamaZenzaburo TozukaKunio MomiyamaYasushi NakamuraKennichi Kakudo
    • Rieko GotoShohei ShioyamaZenzaburo TozukaKunio MomiyamaYasushi NakamuraKennichi Kakudo
    • G01N33/68G06F19/00
    • G01N33/6848
    • A screening method for a specific protein in a proteome analysis comprises: (a) obtaining samples containing a protein or protein digest from a cell or tissue in a specific group and a control group; (b) analyzing the samples obtained in the step (a) with a mass spectrometer, thereby obtaining mass spectrometry data; (c) analyzing the mass spectrometry data obtained in the step (b) using an arbitrary database searching software, thereby acquiring a protein list containing items for specifying proteins and indexes for identifying the proteins, for each of the samples; (d) averaging values of the indexes for each of the items in all of the protein lists acquired in the step (c), and acquiring protein list models of the specific group and the control group, containing the average values of the indexes; (e) calculating a difference between the average values for each of the items, between the protein list models of the specific group and the control group obtained in the step (d), and acquiring one protein list in which the items are rearranged in the order of the difference between the average values; and (f) selecting a protein with a large difference between the average values, from the protein list acquired in the step (e).
    • 蛋白质组分析中特定蛋白质的筛选方法包括:(a)从特定组和对照组的细胞或组织中获得含有蛋白质或蛋白质消化物的样品; (b)用质谱仪分析步骤(a)中获得的样品,从而获得质谱数据; (c)使用任意的数据库搜索软件分析步骤(b)中获得的质谱数据,从而获得每个样品获得含有用于指定蛋白质的项目和用于识别蛋白质的指标的蛋白质列表; (d)对步骤(c)中获得的所有蛋白质列表中的每个项目的指标平均值,并获取包含指标的平均值的特定组和对照组的蛋白质表型模型; (e)计算特定组的蛋白质列表模型与步骤(d)中获得的对照组之间的每个项目的平均值之间的差异,并且获取在其中重新排列项目的一个蛋白质列表 平均值之间的差异顺序; 和(f)从步骤(e)中获得的蛋白质列表中选择平均值差异大的蛋白质。