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1. 发明授权

US09416364B2 Reverse beta oxidation pathway 有权
标题翻译：反向β氧化途径
公开(公告)号：US09416364B2
公开(公告)日：2016-08-16
申请号：US13983885
申请日：2012-02-07
申请人： Ramon Gonzalez , James Clomburg , Clementina Dellomonaco , Elliot N. Miller
发明人： Ramon Gonzalez , James Clomburg , Clementina Dellomonaco , Elliot N. Miller
IPC分类号： C12N15/70 , C12P5/02 , C12P7/40 , C12P7/04 , C12P7/16 , C12P7/42 , C12P7/64
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/001 , C12N9/1029 , C12N9/88 , C12N15/70 , C12P5/02 , C12P5/026 , C12P7/04 , C12P7/40 , C12P7/42 , C12P7/6409 , C12Y101/01035 , C12Y102/0105 , C12Y103/99003 , C12Y203/01 , C12Y402/01017 , Y02E50/10 , Y02P20/52
摘要： The invention relates to recombinant microorganisms that have been engineered to produce various chemicals using genes that have been repurposed to create a reverse beta oxidation pathway. Generally speaking, the beta oxidation cycle is expressed and driven in reverse by modifying various regulation points for as many cycles as needed, and then the CoA thioester intermediates are converted to useful products by the action of termination enzymes.
摘要翻译：本发明涉及重组微生物，其已被工程改造成使用已被重新利用以产生反向β氧化途径的基因产生各种化学物质。一般来说，通过根据需要修改多个循环的各种调节点，反向表达和驱动β氧化循环，然后通过终止酶的作用将CoA硫酯中间体转化成有用的产物。

2. 发明申请

US20130316413A1 REVERSE BETA OXIDATION PATHWAY 有权
标题翻译：反向氧化途径
公开(公告)号：US20130316413A1
公开(公告)日：2013-11-28
申请号：US13983885
申请日：2012-02-07
申请人： Ramon Gonzalez , James Clomburg , Clementina Dellomonaco , Elliot N. Miller
发明人： Ramon Gonzalez , James Clomburg , Clementina Dellomonaco , Elliot N. Miller
IPC分类号： C12N15/70
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/001 , C12N9/1029 , C12N9/88 , C12N15/70 , C12P5/02 , C12P5/026 , C12P7/04 , C12P7/40 , C12P7/42 , C12P7/6409 , C12Y101/01035 , C12Y102/0105 , C12Y103/99003 , C12Y203/01 , C12Y402/01017 , Y02E50/10 , Y02P20/52
摘要： The invention relates to recombinant microorganisms that have been engineered to produce various chemicals using genes that have been repurposed to create a reverse beta oxidation pathway. Generally speaking, the beta oxidation cycle is expressed and driven in reverse by modifying various regulation points for as many cycles as needed, and then the CoA thioester intermediates are converted to useful products by the action of termination enzymes.
摘要翻译：本发明涉及重组微生物，其已被工程改造成使用已被重新利用以产生反向β氧化途径的基因产生各种化学物质。一般来说，通过根据需要修改多个循环的各种调节点，反向表达和驱动β氧化循环，然后通过终止酶的作用将CoA硫酯中间体转化为有用的产物。

3. 发明申请

US20200347423A1 Bioconversion of Short-Chain Hydrocarbons to Fuels and Chemicals 审中-公开
公开(公告)号：US20200347423A1
公开(公告)日：2020-11-05
申请号：US16878032
申请日：2020-05-19
申请人： Ramon Gonzalez , James Clomburg , Alexander Chou
发明人： Ramon Gonzalez , James Clomburg , Alexander Chou
IPC分类号： C12P19/32 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/46 , C12N9/04 , C12N9/02 , C12N9/00 , C12P7/62 , C12N9/10 , C12N9/88 , C12N9/90 , C12P5/02 , C12P7/42 , C12P9/00 , C12P13/00 , C12P13/04
摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

4. 发明授权

US11913049B2 Bioconversion of short-chain hydrocarbons to fuels and chemicals 有权
公开(公告)号：US11913049B2
公开(公告)日：2024-02-27
申请号：US16878032
申请日：2020-05-19
申请人： Ramon Gonzalez , James Clomburg , Alexander Chou
发明人： Ramon Gonzalez , James Clomburg , Alexander Chou
IPC分类号： C12N9/00 , C12P19/32 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/46 , C12N9/04 , C12N9/02 , C12P7/625 , C12N9/10 , C12N9/88 , C12N9/90 , C12P5/02 , C12P7/42 , C12P9/00 , C12P13/00 , C12P13/04
CPC分类号： C12P19/32 , C12N9/00 , C12N9/001 , C12N9/0006 , C12N9/0008 , C12N9/0077 , C12N9/13 , C12N9/88 , C12N9/90 , C12N9/93 , C12P5/02 , C12P5/026 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/625 , C12P9/00 , C12P13/001 , C12P13/04 , C12Y101/01 , C12Y101/03039 , C12Y102/01 , C12Y102/04001 , C12Y103/05001 , C12Y103/99 , C12Y108/01004 , C12Y114/15003 , C12Y203/01012 , C12Y203/01054 , C12Y207/01031 , C12Y208/03 , C12Y401/00 , C12Y401/01 , C12Y401/01047 , C12Y401/03024 , C12Y402/01 , C12Y402/01002 , C12Y402/01011 , C12Y501/99001 , C12Y501/99002 , C12Y504/02 , C12Y504/99 , C12Y504/99001 , C12Y504/99002 , C12Y602/01 , C12Y602/01005 , C12Y604/01003 , Y02E50/30
摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

5. 发明申请

US20220112477A1 BIOSYNTHESIS OF PRODUCTS FROM 1-CARBON COMPOUNDS 有权
公开(公告)号：US20220112477A1
公开(公告)日：2022-04-14
申请号：US17510641
申请日：2021-10-26
申请人： Ramon Gonzalez , Alexander Chou , James Clomburg
发明人： Ramon Gonzalez , Alexander Chou , James Clomburg
IPC分类号： C12N9/88 , C12N15/52 , C12P11/00 , C12P7/24 , C12P13/00 , C12P5/02 , C12P7/40 , C12P1/04 , C12P7/04 , C12N15/70 , C12P7/20
摘要： An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.

6. 发明授权

US11186834B2 Biosynthesis of products from 1-carbon compounds 有权
公开(公告)号：US11186834B2
公开(公告)日：2021-11-30
申请号：US15523284
申请日：2015-10-29
申请人： Ramon Gonzalez , Alexander Chou , James Clomburg
发明人： Ramon Gonzalez , Alexander Chou , James Clomburg
IPC分类号： C12P7/40 , C12P7/44 , C12P7/18 , C12N9/16 , C12N9/04 , C12N9/88 , C12N15/52 , C12P11/00 , C12P7/24 , C12P13/00 , C12P5/02 , C12P1/04 , C12P7/04 , C12N15/70 , C12P7/20
摘要： An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.

7. 发明公开

US20240309417A1 Bioconversion of Short-Chain Hydrocarbons to Fuels and Chemicals 审中-公开
公开(公告)号：US20240309417A1
公开(公告)日：2024-09-19
申请号：US18413954
申请日：2024-01-16
申请人： Ramon Gonzalez
发明人： Ramon Gonzalez , James Clomburg , Alexander Chou
IPC分类号： C12P19/32 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/88 , C12N9/90 , C12P5/02 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/625 , C12P9/00 , C12P13/00 , C12P13/04
CPC分类号： C12P19/32 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0077 , C12N9/13 , C12N9/88 , C12N9/90 , C12N9/93 , C12P5/02 , C12P5/026 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/625 , C12P9/00 , C12P13/001 , C12P13/04 , C12Y101/01 , C12Y101/03039 , C12Y102/01 , C12Y102/04001 , C12Y103/05001 , C12Y103/99 , C12Y108/01004 , C12Y114/15003 , C12Y203/01012 , C12Y203/01054 , C12Y207/01031 , C12Y208/03 , C12Y401/00 , C12Y401/01 , C12Y401/01047 , C12Y401/03024 , C12Y402/01 , C12Y402/01002 , C12Y402/01011 , C12Y501/99001 , C12Y501/99002 , C12Y504/02 , C12Y504/99 , C12Y504/99001 , C12Y504/99002 , C12Y602/01 , C12Y602/01005 , C12Y604/01003 , Y02E50/30
摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

8. 发明授权

US11781120B2 Biosynthesis of polyketides 有权
公开(公告)号：US11781120B2
公开(公告)日：2023-10-10
申请号：US16930220
申请日：2020-07-15
申请人： Ramon Gonzalez
发明人： Ramon Gonzalez , Seokjung Cheong , James M. Clomburg
IPC分类号： C12N9/10 , C12P15/00 , C12P17/06
CPC分类号： C12N9/1025 , C12P17/06 , C12Y203/01174
摘要： This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the β-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the β-keto group to β-hydroxy group. Hydratase converts the β-hydroxy group to α-β-double-bond. Reductase converts the α-β-double-bond to single bond. Spontaneous or thioesterase catalyzed termination reaction terminates the elongation of polyketide chain of polyketoacyl-CoA at any point through CoA removal and spontaneous reactions rearrange the structure, generating the final functional polyketide products.

9. 发明授权

US11697830B2 Iterative platform for the synthesis of alpha functionalized products 有权
公开(公告)号：US11697830B2
公开(公告)日：2023-07-11
申请号：US16818642
申请日：2020-03-13
申请人： Ramon Gonzalez , James M. Clomburg , Seokjung Cheong
发明人： Ramon Gonzalez , James M. Clomburg , Seokjung Cheong
IPC分类号： C12N9/02 , C12P7/42 , C12N9/00 , C12N9/10 , C12N9/04 , C12N9/16 , C12N9/88
CPC分类号： C12P7/42 , C12N9/001 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12Y101/01036 , C12Y102/0101 , C12Y103/01044 , C12Y402/01119
摘要： The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized β-keto acyl-CoA. Dehydrogenase converts alpha-functionalized β-keto acyl-CoA to alpha-functionalized β-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized β-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting alpha-functionalized acyl-CoA as primer and the aforementioned alpha-functionalized extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four alpha-functionalized CoA thioester intermediates terminate the platform and generate various alpha-functionalized carboxylic acids, alcohols and amines with different β-reduction degree.

10. 发明授权

US08691552B2 Microaerobic cultures for converting glycerol to chemicals 失效
标题翻译：用于将甘油转化为化学物质的微生物培养物
公开(公告)号：US08691552B2
公开(公告)日：2014-04-08
申请号：US13126499
申请日：2009-10-28
申请人： Ramon Gonzalez , Paul Campbell
发明人： Ramon Gonzalez , Paul Campbell
IPC分类号： C12N1/20
CPC分类号： C12P7/46 , C12P7/065 , C12P7/52 , C12P7/56 , C12P13/06 , C12R1/19 , Y02E50/17
摘要： Glycerol or other reduced carbon sources may be used as a feedstock for the microbial production of chemical products under certain microaerobic conditions. For example, such production may occur under microaerobic or microrespiratory conditions in which electron acceptors are consumed in the reaction as quickly as they are added. In such reactions, the reaction product is at least as reduced as carbon source. Further, during such a reaction, at least some of the carbon source is used to generate cell mass. In addition, microorganisms with modified genomes are provided for carrying out the methods herein.
摘要翻译：甘油或其他还原碳源可用作在某些微量厌氧条件下微生物生产化学产品的原料。例如，这种生产可以在微需氧或微呼吸条件下发生，其中电子受体在反应中被消耗，就像加入它们一样快。在这种反应中，反应产物至少与碳源一样还原。此外，在这种反应期间，使用至少一些碳源来生成电池块。此外，提供了具有修饰基因组的微生物用于实施本文的方法。

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