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1. 发明授权

US08691552B2 Microaerobic cultures for converting glycerol to chemicals 失效
标题翻译：用于将甘油转化为化学物质的微生物培养物
公开(公告)号：US08691552B2
公开(公告)日：2014-04-08
申请号：US13126499
申请日：2009-10-28
申请人： Ramon Gonzalez , Paul Campbell
发明人： Ramon Gonzalez , Paul Campbell
IPC分类号： C12N1/20
CPC分类号： C12P7/46 , C12P7/065 , C12P7/52 , C12P7/56 , C12P13/06 , C12R1/19 , Y02E50/17
摘要： Glycerol or other reduced carbon sources may be used as a feedstock for the microbial production of chemical products under certain microaerobic conditions. For example, such production may occur under microaerobic or microrespiratory conditions in which electron acceptors are consumed in the reaction as quickly as they are added. In such reactions, the reaction product is at least as reduced as carbon source. Further, during such a reaction, at least some of the carbon source is used to generate cell mass. In addition, microorganisms with modified genomes are provided for carrying out the methods herein.
摘要翻译：甘油或其他还原碳源可用作在某些微量厌氧条件下微生物生产化学产品的原料。例如，这种生产可以在微需氧或微呼吸条件下发生，其中电子受体在反应中被消耗，就像加入它们一样快。在这种反应中，反应产物至少与碳源一样还原。此外，在这种反应期间，使用至少一些碳源来生成电池块。此外，提供了具有修饰基因组的微生物用于实施本文的方法。

2. 发明申请

US20110250654A1 MICROAEROBIC CULTURES FOR CONVERTING GLYCEROL TO CHEMICALS 失效
标题翻译：将甘油转化为化学品的微生物培养基
公开(公告)号：US20110250654A1
公开(公告)日：2011-10-13
申请号：US13126499
申请日：2009-10-28
申请人： Ramon Gonzalez , Paul Campbell
发明人： Ramon Gonzalez , Paul Campbell
IPC分类号： C12P13/06 , C12P13/04 , C12P17/02 , C12P7/40 , C12P7/56 , C12N1/21 , C12P7/46 , C12P7/02 , C12P7/06 , C12P1/04 , C12P1/02 , C12P1/00 , C12P7/52
CPC分类号： C12P7/46 , C12P7/065 , C12P7/52 , C12P7/56 , C12P13/06 , C12R1/19 , Y02E50/17
摘要： Glycerol or other reduced carbon sources may be used as a feedstock for the microbial production of chemical products under certain microaerobic conditions. For example, such production may occur under microaerobic or microrespiratory conditions in which electron acceptors are consumed in the reaction as quickly as they are added. In such reactions, the reaction product is at least as reduced as carbon source. Further, during such a reaction, at least some of the carbon source is used to generate cell mass. In addition, microorganisms with modified genomes are provided for carrying out the methods herein.
摘要翻译：甘油或其他还原碳源可用作在某些微量厌氧条件下微生物生产化学产品的原料。例如，这种生产可以在微需氧或微呼吸条件下发生，其中电子受体在反应中被消耗，就像加入它们一样快。在这种反应中，反应产物至少与碳源一样还原。此外，在这种反应期间，使用至少一些碳源来生成电池块。此外，提供了具有修饰基因组的微生物用于实施本文的方法。

3. 发明申请

US20110229942A1 Microbial Conversion of Oils and Fatty Acids to High-Value Chemicals 审中-公开
标题翻译：油和脂肪酸微生物转化为高价值化学品
公开(公告)号：US20110229942A1
公开(公告)日：2011-09-22
申请号：US12747436
申请日：2008-12-15
申请人： Paul Campbell , Ramon Gonzalez
发明人： Paul Campbell , Ramon Gonzalez
IPC分类号： C12P17/04 , C12N1/21 , C12N1/15 , C12N1/19 , C12P7/06 , C12P7/54 , C12P7/46 , C12P7/18 , C12P7/52 , C12P7/28 , C12P7/62 , C12P7/04 , C12P7/16 , C12P7/42 , C12P13/00
CPC分类号： C12P7/065 , C12N1/20 , C12P7/04 , C12P7/16 , C12P7/18 , C12P7/28 , C12P7/42 , C12P7/46 , C12P7/52 , C12P7/54 , C12P7/62 , C12P13/001 , C12P17/04 , Y02E50/10 , Y02E50/17
摘要： Microorganisms for the production of high-value chemicals from free fatty acids are provided. The microorganisms comprise genetic mutations that alter fatty acid metabolism. The genetic mutations include a mutation or deletion of a fadR gene in which the FadR enzyme activity is partially or substantially eliminated and a mutation in an atoC gene that provides overexpression of the microorganism's ato operon. Methods of using the microorganisms to produce high-value chemicals are also provided. The high-value chemicals include ethanol, methyl acetate, succinate, gamma-butyrolactone, 1,4-butanediol, acetone, iso-propanol, butyrate, butanol, mevalonate, propionate, ethanolamine and 1,2-propanediol.
摘要翻译：提供了用于从游离脂肪酸生产高价值化学物质的微生物。微生物包括改变脂肪酸代谢的遗传突变。遗传突变包括FadR基因的突变或缺失，其中FadR酶活性被部分或基本上消除，并且atoC基因中的突变提供微生物的操纵子的过表达。还提供了使用微生物生产高价值化学品的方法。高价值化学品包括乙醇，乙酸甲酯，琥珀酸酯，γ-丁内酯，1,4-丁二醇，丙酮，异丙醇，丁酸酯，丁醇，甲羟戊酸，丙酸酯，乙醇胺和1,2-丙二醇。

4. 发明公开

US20240309417A1 Bioconversion of Short-Chain Hydrocarbons to Fuels and Chemicals 审中-公开
公开(公告)号：US20240309417A1
公开(公告)日：2024-09-19
申请号：US18413954
申请日：2024-01-16
申请人： Ramon Gonzalez
发明人： Ramon Gonzalez , James Clomburg , Alexander Chou
IPC分类号： C12P19/32 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/88 , C12N9/90 , C12P5/02 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/625 , C12P9/00 , C12P13/00 , C12P13/04
CPC分类号： C12P19/32 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0077 , C12N9/13 , C12N9/88 , C12N9/90 , C12N9/93 , C12P5/02 , C12P5/026 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/625 , C12P9/00 , C12P13/001 , C12P13/04 , C12Y101/01 , C12Y101/03039 , C12Y102/01 , C12Y102/04001 , C12Y103/05001 , C12Y103/99 , C12Y108/01004 , C12Y114/15003 , C12Y203/01012 , C12Y203/01054 , C12Y207/01031 , C12Y208/03 , C12Y401/00 , C12Y401/01 , C12Y401/01047 , C12Y401/03024 , C12Y402/01 , C12Y402/01002 , C12Y402/01011 , C12Y501/99001 , C12Y501/99002 , C12Y504/02 , C12Y504/99 , C12Y504/99001 , C12Y504/99002 , C12Y602/01 , C12Y602/01005 , C12Y604/01003 , Y02E50/30
摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

5. 发明授权

US11781120B2 Biosynthesis of polyketides 有权
公开(公告)号：US11781120B2
公开(公告)日：2023-10-10
申请号：US16930220
申请日：2020-07-15
申请人： Ramon Gonzalez
发明人： Ramon Gonzalez , Seokjung Cheong , James M. Clomburg
IPC分类号： C12N9/10 , C12P15/00 , C12P17/06
CPC分类号： C12N9/1025 , C12P17/06 , C12Y203/01174
摘要： This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the β-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the β-keto group to β-hydroxy group. Hydratase converts the β-hydroxy group to α-β-double-bond. Reductase converts the α-β-double-bond to single bond. Spontaneous or thioesterase catalyzed termination reaction terminates the elongation of polyketide chain of polyketoacyl-CoA at any point through CoA removal and spontaneous reactions rearrange the structure, generating the final functional polyketide products.

6. 发明授权

US11697830B2 Iterative platform for the synthesis of alpha functionalized products 有权
公开(公告)号：US11697830B2
公开(公告)日：2023-07-11
申请号：US16818642
申请日：2020-03-13
申请人： Ramon Gonzalez , James M. Clomburg , Seokjung Cheong
发明人： Ramon Gonzalez , James M. Clomburg , Seokjung Cheong
IPC分类号： C12N9/02 , C12P7/42 , C12N9/00 , C12N9/10 , C12N9/04 , C12N9/16 , C12N9/88
CPC分类号： C12P7/42 , C12N9/001 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12Y101/01036 , C12Y102/0101 , C12Y103/01044 , C12Y402/01119
摘要： The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized β-keto acyl-CoA. Dehydrogenase converts alpha-functionalized β-keto acyl-CoA to alpha-functionalized β-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized β-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting alpha-functionalized acyl-CoA as primer and the aforementioned alpha-functionalized extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four alpha-functionalized CoA thioester intermediates terminate the platform and generate various alpha-functionalized carboxylic acids, alcohols and amines with different β-reduction degree.

7. 发明申请

US20200347423A1 Bioconversion of Short-Chain Hydrocarbons to Fuels and Chemicals 审中-公开
公开(公告)号：US20200347423A1
公开(公告)日：2020-11-05
申请号：US16878032
申请日：2020-05-19
申请人： Ramon Gonzalez , James Clomburg , Alexander Chou
发明人： Ramon Gonzalez , James Clomburg , Alexander Chou
IPC分类号： C12P19/32 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/46 , C12N9/04 , C12N9/02 , C12N9/00 , C12P7/62 , C12N9/10 , C12N9/88 , C12N9/90 , C12P5/02 , C12P7/42 , C12P9/00 , C12P13/00 , C12P13/04
摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

8. 发明授权

US11319562B2 Modified fatty acid biosynthesis with ACP-dependent thiolases 有权
公开(公告)号：US11319562B2
公开(公告)日：2022-05-03
申请号：US16815790
申请日：2020-03-11
申请人： Ramon Gonzalez , James M. Clomburg
发明人： Ramon Gonzalez , James M. Clomburg
IPC分类号： C12N9/00 , C12P7/6409
摘要： Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse β-oxidation cycle.

9. 发明授权

US08334119B2 Anaerobic fermentation of glycerol 有权
标题翻译：甘油的厌氧发酵
公开(公告)号：US08334119B2
公开(公告)日：2012-12-18
申请号：US13347811
申请日：2012-01-11
申请人： Ramon Gonzalez
发明人： Ramon Gonzalez
IPC分类号： C12P7/00
CPC分类号： C12P7/065 , C12P3/00 , C12P7/40 , Y02E50/17
摘要： The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pII, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol.
摘要翻译：本发明涉及在甘油底物上厌氧生长（发酵）培养细菌的合适培养条件。该方法需要在含有高浓度甘油，中性至轻度酸性pII，低水平钾和磷酸盐的培养基中培养具有官能1,2-丙二醇途径和功能性II型甘油脱氢酶 - 二羟基丙酮激酶途径的细菌，以及高水平的CO 2，使得甘油因此转化为所需的产物，例如乙醇，氢气，甲酸盐，琥珀酸盐或1,2-丙二醇。

10. 发明授权

US4679793A Table game 失效
标题翻译：桌面游戏
公开(公告)号：US4679793A
公开(公告)日：1987-07-14
申请号：US838308
申请日：1986-03-10
申请人： Ramon Gonzalez
发明人： Ramon Gonzalez
IPC分类号： A63F9/02 , A63F7/24
CPC分类号： A63F9/02
摘要： A table game as provided to be played by up to four players and to simulate the action of a boxing contest, or the like. The game includes a flat board with players and markers and an adjustable pendulum used by the players to attempt to knock over the opponents' game pieces.
摘要翻译：由多达四名玩家提供的桌面游戏，并模拟拳击比赛的动作等。该游戏包括一个带有玩家和标记的平板，以及玩家使用的可调节摆锤，试图敲击对手的游戏。

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