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1. 发明申请

US20110091922A1 Bio-Matrix Stretcher 有权
标题翻译：生物基质担架
公开(公告)号：US20110091922A1
公开(公告)日：2011-04-21
申请号：US12675882
申请日：2008-08-28
申请人： Ramaswamy Krishnan , Chan Young Park , Jeffrey Fredberg , Fei Liu , Justin Mih , Daniel Tschumperlin
发明人： Ramaswamy Krishnan , Chan Young Park , Jeffrey Fredberg , Fei Liu , Justin Mih , Daniel Tschumperlin
IPC分类号： C12Q1/02 , C12M1/34
CPC分类号： C12N5/0068 , C12M23/12 , C12N2533/30
摘要： Embodiments provide techniques for measuring and characterizing the dynamics of cell traction forces. Tunable elastic gel substrates can be disposed in multi-well plates. The gels can be of a uniform predetermined thickness. A multi-well plate can be loaded with gels of different shear moduli. An array of punch indenters can be attached to a loading platen such that the each indenter is aligned to a gel substrate. The indenters can apply tensile or compressive strains to the gel substrates. The magnitude, duration, and frequency of the strain can be controlled by a motor assembly coupled to a control system. The apparatus can be disposed in an incubator for long term cell culture experiments. The cell culture can be observed while a strain is applied. A ring-shaped indenter can be mounted on a microscope, coaxial to the objective lens, and lowered by a calibrated amount onto the underlying gel.
摘要翻译：实施例提供了用于测量和表征细胞牵引力的动力学的技术。可调谐的弹性凝胶基材可以设置在多孔板中。凝胶可以具有均匀的预定厚度。多孔板可装载不同剪切模量的凝胶。可以将一组冲头压头连接到加载压板，使得每个压头与凝胶基板对准。压痕可以对凝胶基质施加拉伸或压缩应变。应变的幅度，持续时间和频率可以通过耦合到控制系统的电机组件来控制。该装置可以放置在用于长期细胞培养实验的培养箱中。可以在应用菌株时观察细胞培养物。环形压头可以安装在与物镜同轴的显微镜上，并通过校准量下降到下面的凝胶上。

2. 发明授权

US08993312B2 Bio-matrix stretcher 有权
标题翻译：生物基质担架
公开(公告)号：US08993312B2
公开(公告)日：2015-03-31
申请号：US12675882
申请日：2008-08-28
申请人： Ramaswamy Krishnan , Chan Young Park , Jeffrey Fredberg , Fei Liu , Justin Mih , Daniel Tschumperlin
发明人： Ramaswamy Krishnan , Chan Young Park , Jeffrey Fredberg , Fei Liu , Justin Mih , Daniel Tschumperlin
IPC分类号： C12Q1/02 , C12M1/34 , C12N5/00 , C12M1/32
CPC分类号： C12N5/0068 , C12M23/12 , C12N2533/30
摘要： Embodiments provide techniques for measuring and characterizing the dynamics of cell traction forces. Tunable elastic gel substrates can be disposed in multi-well plates. The gels can be of a uniform predetermined thickness. A multi-well plate can be loaded with gels of different shear moduli. An array of punch indenters can be attached to a loading platen such that the each indenter is aligned to a gel substrate. The indenters can apply tensile or compressive strains to the gel substrates. The magnitude, duration, and frequency of the strain can be controlled by a motor assembly coupled to a control system. The apparatus can be disposed in an incubator for long term cell culture experiments. The cell culture can be observed while a strain is applied. A ring-shaped indenter can be mounted on a microscope, coaxial to the objective lens, and lowered by a calibrated amount onto the underlying gel.
摘要翻译：实施例提供了用于测量和表征细胞牵引力的动力学的技术。可调谐的弹性凝胶基材可以设置在多孔板中。凝胶可以具有均匀的预定厚度。多孔板可装载不同剪切模量的凝胶。可以将一组冲头压头连接到加载压板，使得每个压头与凝胶基板对准。压痕可以对凝胶基质施加拉伸或压缩应变。应变的幅度，持续时间和频率可以通过耦合到控制系统的电机组件来控制。该装置可以放置在用于长期细胞培养实验的培养箱中。可以在应用菌株时观察细胞培养物。环形压头可以安装在与物镜同轴的显微镜上，并通过校准量下降到下面的凝胶上。

3. 发明授权

US08871499B2 Multi-well culture plate comprising gels with different shear modulus 有权
标题翻译：包含不同剪切模量的凝胶的多孔培养板
公开(公告)号：US08871499B2
公开(公告)日：2014-10-28
申请号：US12675839
申请日：2008-08-28
申请人： Daniel Tschumperlin , Fei Liu , Justin Mih
发明人： Daniel Tschumperlin , Fei Liu , Justin Mih
IPC分类号： C12M3/00 , C12Q1/02 , C12Q1/04
CPC分类号： C12N5/0068 , C12M23/12 , C12N2533/30
摘要： A multi-well plate can be loaded with a range of compliant substrates. Commerically-available assays can be used to test cellular responses across a plate with shear modulus from 50 to 51200 Pascals. Cells can be grown in the plates, and can be manipulated and analyzed. Hydrogels can be attached to the bottom of a well. The plates can support the attachment and growth of different cell types and can be compatible with standard 96-well and 384-well plate assays. The mechanical properties of the hydrogels can be reproducible and stable to increase the shelf life of the substrate. The hydrogel can be compatible with growth of a variety of cell types, various attachment ligands such as collagen I, collagen IV, fibronectin, vitronectin, laminin, or RGD peptides and can be coupled to the gel surface.
摘要翻译：多孔板可以装载一系列柔顺基板。商业上可用的测定可以用于测试横跨板的细胞反应，剪切模量为50至51200帕斯卡。细胞可以在板中生长，并且可以被操纵和分析。水凝胶可以附着在井的底部。板可以支持不同细胞类型的附着和生长，并且可以与标准的96孔和384孔板测定相兼容。水凝胶的机械性能可重现和稳定，以增加基材的保质期。水凝胶可以与各种细胞类型，各种附着配体如胶原I，胶原IV，纤连蛋白，玻连蛋白，层粘连蛋白或RGD肽的生长相容并且可以与凝胶表面偶联。

4. 发明申请

US20110104798A1 Compliant Surface Multi-Well Culture Plate 有权
标题翻译：符合表面多孔培养板
公开(公告)号：US20110104798A1
公开(公告)日：2011-05-05
申请号：US12675839
申请日：2008-08-28
申请人： Daniel Tschumperlin , Fei Liu , Justin Mih
发明人： Daniel Tschumperlin , Fei Liu , Justin Mih
IPC分类号： C12N5/071 , C12M3/00 , C40B60/00 , B05D3/00
CPC分类号： C12N5/0068 , C12M23/12 , C12N2533/30
摘要： A multi-well plate can be loaded with a range of compliant substrates. Commerically-available assays can be used to test cellular responses across a plate with shear modulus from 50 to 51200 Pascals. Cells can be grown in the plates, and can be manipulated and analyzed. Hydrogels can be attached to the bottom of a well. The plates can support the attachment and growth of different cell types and can be compatible with standard 96-well and 384-well plate assays. The mechanical properties of the hydrogels can be reproducible and stable to increase the shelf life of the substrate. The hydrogel can be compatible with growth of a variety of cell types, various attachment ligands such as collagen I, collagen IV, fibronectin, vitronectin, laminin, or RGD peptides and can be coupled to the gel surface.
摘要翻译：多孔板可以装载一系列柔顺基板。商业上可用的测定可以用于测试横跨板的细胞反应，剪切模量为50至51200帕斯卡。细胞可以在板中生长，并且可以被操纵和分析。水凝胶可以附着在井的底部。板可以支持不同细胞类型的附着和生长，并且可以与标准的96孔和384孔板测定相兼容。水凝胶的机械性能可重现和稳定，以增加基材的保质期。水凝胶可以与各种细胞类型，各种附着配体如胶原I，胶原IV，纤连蛋白，玻连蛋白，层粘连蛋白或RGD肽的生长相容并且可以与凝胶表面偶联。

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