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1. 发明申请

US20080318288A1 Enzymatic Reduction for Producing Optically Active Alcohols 有权
标题翻译：用于生产光活性醇的酶减量
公开(公告)号：US20080318288A1
公开(公告)日：2008-12-25
申请号：US12158390
申请日：2006-12-13
申请人： Rainer Stürmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
IPC分类号： C12P17/00 , C12N9/14
CPC分类号： C07D333/16 , C07D333/20 , C12P7/02 , C12P11/00 , C12P41/002
摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.
摘要翻译：一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

2. 发明授权

US07888080B2 Enzymatic reduction for producing optically active alcohols 有权
标题翻译：用于制备光学活性醇的酶还原
公开(公告)号：US07888080B2
公开(公告)日：2011-02-15
申请号：US12158390
申请日：2006-12-13
申请人： Rainer Stürmer , Maria Keβeler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Maria Keβeler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
IPC分类号： C12P17/00 , C12N9/14
CPC分类号： C07D333/16 , C07D333/20 , C12P7/02 , C12P11/00 , C12P41/002
摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.
摘要翻译：一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

3. 发明授权

US08012725B2 Method for the production of optically-active alcohols 有权
标题翻译：光学活性醇的制造方法
公开(公告)号：US08012725B2
公开(公告)日：2011-09-06
申请号：US11667721
申请日：2005-11-15
申请人： Rainer Stürmer , Bernhard Hauer , Dejana Drew , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Bernhard Hauer , Dejana Drew , Michael Breuer , Hartwig Schröder
IPC分类号： C12P7/16 , C12P7/02 , C12P7/04 , C12Q1/26 , C12Q1/32 , C12N1/00 , C12N1/20 , C12N9/02 , C07H21/04
CPC分类号： C12P7/16 , Y02E50/10
摘要： The present invention relates to enzymic processes for preparing S-butan-2-ol; and to enzymes for carrying out said processes; to nucleic acid sequences coding for said enzymes, to expression cassettes, vectors and recombinant hosts containing said nucleic acid sequences.
摘要翻译：本发明涉及制备S-丁-2-醇的酶法; 和用于进行所述方法的酶; 编码所述酶的核酸序列，含有所述核酸序列的表达盒，载体和重组宿主。

4. 发明授权

US07498448B2 Methods for the production of 3-methylamino-1-(thiene-2-yl)-propane-1-ol 有权
标题翻译： 3-甲基氨基-1-（噻吩-2-基） - 丙-1-醇的制备方法
公开(公告)号：US07498448B2
公开(公告)日：2009-03-03
申请号：US10573130
申请日：2004-09-30
申请人： Rainer Stürmer , Maria Keβeler , Bernhard Hauer , Thomas Friedrich , Michael Breuer
发明人： Rainer Stürmer , Maria Keβeler , Bernhard Hauer , Thomas Friedrich , Michael Breuer
IPC分类号： C07D333/12
CPC分类号： C07D333/16
摘要： The present invention relates to enzymic and nonenzymic processes for preparing 3-methylamino-1-(thien-2-yl)propan-1-ol; as well as to enzymes for implementing these processes; and to nucleic acid sequences encoding these enzymes, to expression cassettes containing these nucleic acid sequences, to vectors and to recombinant hosts.
摘要翻译：本发明涉及制备3-甲基氨基-1-（噻吩-2-基）丙-1-醇的酶和非酶法; 以及用于实施这些过程的酶; 以及编码这些酶的核酸序列，含有这些核酸序列的表达盒，载体和重组宿主。

5. 发明申请

US20080206824A1 Method For Producing Optically Active Alcohols From Alkanones Using a Dehydrogenase of Azoarcus 有权
标题翻译：使用Azoarcus脱氢酶从烷醇生产光活性醇的方法
公开(公告)号：US20080206824A1
公开(公告)日：2008-08-28
申请号：US11579292
申请日：2005-05-04
申请人： Rainer Sturmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schroder
发明人： Rainer Sturmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schroder
IPC分类号： C12P17/00 , C12P7/22
CPC分类号： C07D333/22 , C07D333/16 , C12P17/00
摘要： The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.
摘要翻译：本发明涉及一种制备式（I）的光学活性链烷醇的方法，其中n为0-5的整数; Cyc表示任选取代的单核或多核，饱和或不饱和的碳环或杂环，R 1表示卤素，SH，OH，NO 2，NR 2/3/3或3/3/3/3/4/4 / 其中R 2，R 3和R 4独立地表示H或低级烷基或低级烷氧基，X - 表示抗衡离子。根据本发明，将选自脱氢酶，醛还原酶和羰基还原酶的酶（E）在含有式（II）的烷酮的培养基中温育，其中n，Cyc和R 1 如上所述，在还原当量的存在下。将式（II）化合物酶还原成式（I）化合物，并且通过使用酶（E）使牺牲醇与相应的牺牲酮反应，并且至少部分除去牺牲酮来再生反应期间消耗的还原当量从反应介质中分离出如此制备的产物（I）。

6. 发明授权

US06998258B1 L-pantolactone-hydrolase and a method for producing D-pantolactone 失效
标题翻译： L-泛酸内酯水解酶和D-泛酸内酯的制备方法
公开(公告)号：US06998258B1
公开(公告)日：2006-02-14
申请号：US10111451
申请日：2000-10-20
申请人： Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Ralf Mattes
发明人： Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Ralf Mattes
IPC分类号： C12N9/14 , C12N15/55 , C12P7/26
CPC分类号： C12P17/04 , C12N9/18 , C12P41/005
摘要： The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.
摘要翻译：本发明涉及具有水解L-泛酸内酯的酶活性的蛋白质。本发明还涉及编码这些蛋白质的核酸，核酸构建体，载体，遗传修饰的微生物以及制备D-泛酸内酯的方法。

7. 发明申请

US20060259999A1 Modified nitrilases and their use in methods for the production of carboxylic acids 有权
标题翻译：改性腈水解酶及其在羧酸生产方法中的应用
公开(公告)号：US20060259999A1
公开(公告)日：2006-11-16
申请号：US10546611
申请日：2004-02-24
申请人： Thomas Zelinski , Maria Kesseler , Bernhard Hauer , Thomas Friedrich
发明人： Thomas Zelinski , Maria Kesseler , Bernhard Hauer , Thomas Friedrich
IPC分类号： C12P7/40 , C12N9/80 , C12N15/87
CPC分类号： C12N9/80 , C12N9/78 , C12N15/8274 , C12P7/40 , C12P7/42
摘要： The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.
摘要翻译：本发明涉及具有改进的基板接受性的新腈水解酶，获得它们的方法和使用上述腈水解酶。所述腈水解酶由多肽序列编码，其在与野生型产碱杆菌粪便中的位置296相对应的位置包含不是酪氨酸的氨基酸。本发明进一步涉及编码所述腈水解酶的核酸序列和氨基酸序列，包含所述核酸序列，包含核酸序列或exPression构建体的载体的表达构建体，包括核酸序列，表达构建体或生物体向量本发明还涉及从外消旋腈制备羧酸，优选取代的手性羧酸的方法。

8. 发明申请

US20100035315A1 PROCESS FOR ENZYMATIC REDUCTION OF ALKENE DERIVATIVES 失效
标题翻译：碱性衍生物的酶促还原过程
公开(公告)号：US20100035315A1
公开(公告)日：2010-02-11
申请号：US12514215
申请日：2007-11-13
申请人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
发明人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
IPC分类号： C12P7/26
CPC分类号： C12P7/24 , C12P7/26 , C12P7/40 , C12P7/46 , C12P7/62 , C12P13/008 , C12P17/10 , C12P41/002
摘要： A method for enzymatic preparation of compounds of the general formula (2) from unsaturated alkene derivatives of the general formula (1) by reducing a compound of the formula (1) in the presence of a reductase, comprising at least one of the polypeptide sequences SEQ ID NO: 1, 2 or 3 or having a functionally equivalent polypeptide sequence which is at least 80% identical to SEQ ID NO: 1, 2 or 3.
摘要翻译：一种通过在还原酶的存在下还原式（1）的化合物，从通式（1）的不饱和烯烃衍生物中酶制备通式（2）的化合物的方法，所述方法包括至少一种多肽序列具有与SEQ ID NO：1,2或3至少80％相同的功能等同多肽序列的SEQ ID NO：1,2或3。

9. 发明授权

US08313923B2 Process for enzymatic reduction of alkene derivatives 失效
标题翻译：烯烃衍生物的酶还原方法
公开(公告)号：US08313923B2
公开(公告)日：2012-11-20
申请号：US12514215
申请日：2007-11-13
申请人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
发明人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
IPC分类号： C12P7/26
CPC分类号： C12P7/24 , C12P7/26 , C12P7/40 , C12P7/46 , C12P7/62 , C12P13/008 , C12P17/10 , C12P41/002
摘要： A method for enzymatic preparation of compounds of the general formula (2) from unsaturated alkene derivatives of the general formula (1) by reducing a compound of the formula (1) in the presence of a reductase, comprising at least one of the polypeptide sequences SEQ ID NO: 1, 2 or 3 or having a functionally equivalent polypeptide sequence which is at least 80% identical to SEQ ID NO: 1, 2 or 3.
摘要翻译：一种通过在还原酶的存在下还原式（1）的化合物，从通式（1）的不饱和烯烃衍生物中酶制备通式（2）的化合物的方法，所述方法包括至少一种多肽序列具有与SEQ ID NO：1,2或3至少80％相同的功能等同多肽序列的SEQ ID NO：1,2或3。

10. 发明授权

US07531331B2 Butynol I esterase 有权
标题翻译：丁醇I酯酶
公开(公告)号：US07531331B2
公开(公告)日：2009-05-12
申请号：US10362530
申请日：2001-08-30
申请人： Bernhard Hauer , Thomas Friedrich , Christoph Nübling , Rainer Stürmer , Wolfgang Ladner
发明人： Bernhard Hauer , Thomas Friedrich , Christoph Nübling , Rainer Stürmer , Wolfgang Ladner
IPC分类号： C12P7/62 , C12P7/64 , C12P21/06 , C12N9/18
CPC分类号： C12N9/16 , C12P41/00
摘要： The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.
摘要翻译：本发明涉及具有酯酶活性，特别是丁酸酯酶活性的编码的核酸序列，表达盒，载体和重组微生物的新型蛋白质，涉及用于制备所述蛋白质的方法及其用于酶，特别是对映选择性酶，酯水解或酯交换有机酯的用途。

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