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1. 发明授权

US07485443B2 Solid-phase reactions 失效
标题翻译：固相反应
公开(公告)号：US07485443B2
公开(公告)日：2009-02-03
申请号：US10197189
申请日：2002-07-17
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
IPC分类号： C12P19/34
CPC分类号： C12Q1/683 , C12Q2565/549 , C12Q2561/109
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译：本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。本发明涉及将核酸连接到固体支持物和修饰核酸的方法。例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。

2. 发明申请

US20050003355A1 Single nucleotide polymorphism analysis using surface invasive cleavage reactions 审中-公开
标题翻译：使用表面侵入性切割反应的单核苷酸多态性分析
公开(公告)号：US20050003355A1
公开(公告)日：2005-01-06
申请号：US10420247
申请日：2003-04-22
申请人： Manchun Lu , Jeff Hall , Michael Shortreed , Liman Wang , Travis Berggren , Priscilla Stevens , David Kelso , Victor Lyamichev , Bruce Neri , Lloyd Smith
发明人： Manchun Lu , Jeff Hall , Michael Shortreed , Liman Wang , Travis Berggren , Priscilla Stevens , David Kelso , Victor Lyamichev , Bruce Neri , Lloyd Smith
IPC分类号： C12Q1/68
CPC分类号： B82Y30/00 , B82Y15/00 , C12Q1/6827 , C12Q1/6837 , C12Q2565/501 , C12Q2561/109 , C12Q2565/101 , C12Q2521/319 , C12Q2565/543 , C12Q2537/143
摘要： Disclosed are a method and a corresponding composition of matter for detecting single nucleotide polymorphisms. The composition of matter includes a metal substrate, a probe oligonucleotide immobilized on the substrate; and an upstream oligonucleotide either immobilized on the substrate or in a solution in contact with the substrate. The probe oligonucleotide and the upstream oligonucleotide participate cooperatively in an invasive cleavage reaction when the substrate is contacted with a cleavage agent and a target nucleic acid. Under proper reactions conditions, the probe oligonucleotide, the upstream oligonucleotide, the target nucleotide, and the cleavage agent cooperative result in the formation of a cleavage product at or near the location of a single nucleotide polymorphism of interest.
摘要翻译：公开了用于检测单核苷酸多态性的方法和相应的物质组成。物质组成包括金属底物，固定在基底上的探针寡核苷酸; 以及固定在基底上或与基底接触的溶液中的上游寡核苷酸。当底物与切割剂和靶核酸接触时，探针寡核苷酸和上游寡核苷酸协同参与侵入性切割反应。在适当的反应条件下，探针寡核苷酸，上游寡核苷酸，靶核苷酸和切割剂协同导致在感兴趣的单核苷酸多态性位置处或附近形成切割产物。

3. 发明申请

US20050164177A1 Reactions on a solid surface 失效
标题翻译：固体表面上的反应
公开(公告)号：US20050164177A1
公开(公告)日：2005-07-28
申请号：US09732622
申请日：2000-12-08
申请人： Bruce Neri , Jeff Hall , Victor Lyamichev , Lloyd Smith
发明人： Bruce Neri , Jeff Hall , Victor Lyamichev , Lloyd Smith
IPC分类号： C12M1/34 , C12N9/22 , C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译：本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。

4. 发明申请

US20070023621A1 Nanoelectromechanical and Microelectromechanical Sensors and Analyzers 有权
标题翻译：纳米机电和微机电传感器和分析仪
公开(公告)号：US20070023621A1
公开(公告)日：2007-02-01
申请号：US11460063
申请日：2006-07-26
申请人： Robert Blick , Michael Westphall , Lloyd Smith
发明人： Robert Blick , Michael Westphall , Lloyd Smith
IPC分类号： H05H3/02
CPC分类号： G01N29/022 , B82Y15/00 , G01N29/036 , G01N2291/0256 , G01N2291/0423 , G01N2291/0427 , G01N2291/106 , H01J49/025 , H01J2329/00
摘要： The present invention provides methods, devices and device components for detecting, sensing and analyzing molecules. Detectors of the present invention provide good detection sensitivity over a wide range of molecular masses ranging from a few Daltons up to 10s of megadaltons, which does not decrease as function of molecular mass. Sensors and analyzers of the present invention detect emission from an array of resonators to determine the molecular masses and/or electric charges of molecules which impact or contact an external surface of a membrane that is used to mount and excite the resonators in the array. Resonators in the array are excited via piezoelectric and/or magnetic excitation of the mounting membrane and, optionally, grid electrodes are used in certain configurations for electrically biasing for the resonator array, and for amplification or suppression of emission from the resonators so as to provide detection and mass/electric charge analysis with good sensitivity and resolution.
摘要翻译：本发明提供用于检测，感测和分析分子的方法，装置和装置组件。本发明的检测器在范围广泛的分子量范围内提供良好的检测灵敏度，范围从几道尔顿至10s兆兆分之一，其不随分子量的功能而降低。本发明的传感器和分析器检测来自谐振器阵列的发射，以确定影响或接触用于安装和激励阵列中的谐振器的膜的外表面的分子的分子质量和/或电荷。阵列中的谐振器通过安装膜的压电和/或磁激励而激发，并且可选地，栅极用于某些构造以用于谐振器阵列的电偏置，以及用于放大或抑制来自谐振器的发射，从而提供检测和质量/电荷分析具有良好的灵敏度和分辨率。

5. 发明申请

US20070102634A1 Electrospray ionization ion source with tunable charge reduction 有权
标题翻译：具有可调电荷还原的电喷雾离子源
公开(公告)号：US20070102634A1
公开(公告)日：2007-05-10
申请号：US11272450
申请日：2005-11-10
申请人： Brian Frey , Lloyd Smith , Michael Westphall
发明人： Brian Frey , Lloyd Smith , Michael Westphall
IPC分类号： H01J49/10
CPC分类号： H01J49/165 , H01J49/0045
摘要： This invention provides methods, devices and device components for preparing ions from liquid samples containing chemical species and methods and devices for analyzing chemical species in liquid samples. The present invention provides an ion source for generating analyte ions having a selected charge state distribution, such as a reduced charged state distribution, that may be effectively interfaced with a variety of charged particle analyzers, including virtually any type of mass spectrometer.
摘要翻译：本发明提供用于从含有化学物质的液体样品制备离子的方法，装置和装置组件以及用于分析液体样品中的化学物质的方法和装置。本发明提供了一种用于产生具有选定的电荷状态分布的分析离子的离子源，所述电荷状态分布例如降低的充电状态分布，其可以有效地与包括实际上任何类型的质谱仪在内的各种带电粒子分析仪接口。

6. 发明授权

US5827657A Direct cloning of PCR amplified nucleic acids 失效
公开(公告)号：US5827657A
公开(公告)日：1998-10-27
申请号：US683237
申请日：1996-07-18
申请人： Corinna Herrnstadt , Joseph M. Fernandez , Lloyd Smith , David A. Mead
发明人： Corinna Herrnstadt , Joseph M. Fernandez , Lloyd Smith , David A. Mead
IPC分类号： C12N15/10 , C12N15/66 , C12N15/70 , C12Q1/68
CPC分类号： C12N15/70 , C12N15/10 , C12N15/66 , C12Q1/6853
摘要： Methods are described for producing recombinant DNA molecules from suitable host vectors and nucleic acids subjected to 3'-terminal transferase activity. In one embodiment, the method takes advantage of the single 3'-deoxy-adenosine monophosphate (dAMP) residues attached to the 3' termini of PCR generated nucleic acids. Vectors are prepared with recognition sequences that afford single 3'-terminal deoxy-thymidine monophosphate (dTMP) residues upon reaction with a suitable restriction enzyme. Thus, PCR generated copies of genes can be directly cloned into the vectors without need for preparing primers having suitable restriction sites therein. The invention also contemplates associated plasmid vectors and kits for implementing the methods.

7. 发明申请

US20080050833A1 Ionizable isotopic labeling reagents for relative quantification by mass spectrometry 有权
标题翻译：用于质谱相对定量的可离子化同位素标记试剂
公开(公告)号：US20080050833A1
公开(公告)日：2008-02-28
申请号：US11726307
申请日：2007-03-21
申请人： Lloyd Smith , Michael Shortreed , Brian Frey , Margaret Phillips , Joshua Coon , Shane Lamos , Casey Krusemark , Peter Belshaw , Madhusudan Patel , Neil Kelleher
发明人： Lloyd Smith , Michael Shortreed , Brian Frey , Margaret Phillips , Joshua Coon , Shane Lamos , Casey Krusemark , Peter Belshaw , Madhusudan Patel , Neil Kelleher
IPC分类号： G01N33/00
CPC分类号： C07B59/001 , C07B59/00 , C07B59/008 , G01N30/7233 , G01N2458/15 , Y10T436/163333 , Y10T436/17
摘要： Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic kit facilitating relative quantification and providing tangible evidence for the existence of specific functional groups. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules, such as biological samples. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. Advantageously, the labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications. Labeled primary and secondary amine and carboxylic acid end-products also generated higher signals concerning mass-spectra than pre-cursor molecules and improved sensitivity. Improved accuracy concerning relative quantification was achieved by mixing heavy and light labeled Arabidopsis extracts in different ratios. Labeling strategy was further employed to ascertain differences in the amounts of amine-containing metabolites for two strains of Arabidopsis seeds.
摘要翻译：通过电喷雾离子化质谱（ESI-MS）对代谢物进行相对定量，其需要同时分析两个或多个样品中多种分析物的机制。标记对特定化合物类别具有反应性的试剂，仅在其同位素试剂盒中有所不同，便于相对定量，并为特定官能团的存在提供有形证据。合成了甲基亚酰亚胺酸的重和轻同位素形式，并用作定量含胺分子（如生物样品）的标记试剂。还合成了重和轻的同位素形式的甲醛和胆胺，并且独立地用作用于定量含胺和含羧酸的分子的标记试剂，例如在生物样品中发现的。有利地，标记的最终产物在常规的液相色谱质谱（LC / MS）应用的正常酸性条件下带正电荷。标记的伯胺和仲胺和羧酸终产物也产生了比前体分子更高的质谱信号，提高了灵敏度。通过以不同比例混合重和轻标记的拟南芥提取物，提高了相对定量的准确性。进一步使用标记策略来确定两种拟南芥种子的含胺代谢物的量的差异。

8. 发明申请

US20060211024A1 Methods for analysis of a nucleic acid sample 审中-公开
标题翻译：分析核酸样品的方法
公开(公告)号：US20060211024A1
公开(公告)日：2006-09-21
申请号：US11372823
申请日：2006-03-09
申请人： Robert Corn , Lloyd Smith , Timothy Burland , Voula Kodoyianni
发明人： Robert Corn , Lloyd Smith , Timothy Burland , Voula Kodoyianni
IPC分类号： C12Q1/68
CPC分类号： B82Y15/00 , B82Y30/00 , C12Q1/6834 , C12Q2561/108 , C12Q2537/149 , C12Q2533/101
摘要： An assay for analysis of a nucleic acid sample is provided. In one embodiment, the assay includes: a) employing an RNA sample to obtain a DNA probe; b) contacting the DNA probe with a substrate containing a surface-immobilized RNA oligonucleotide to produce a surface-immobilized RNA/DNA duplex; and c) detecting RNAseH-dependent cleavage of the surface-immobilized RNA oligonucleotide in the RNA/DNA duplex.
摘要翻译：提供了用于分析核酸样品的测定法。在一个实施方案中，测定包括：a）使用RNA样品获得DNA探针; b）将DNA探针与含有表面固定的RNA寡核苷酸的底物接触以产生表面固定的RNA / DNA双链体; 和c）检测RNA / DNA双链体中表面固定的RNA寡核苷酸的RNAseH依赖性切割。

9. 发明申请

US20050056267A1 COMBINATION RADIANT/CONVECTION GAS COOKING APPLIANCE 有权
标题翻译：组合式辐射/对流气体烹饪器具
公开(公告)号：US20050056267A1
公开(公告)日：2005-03-17
申请号：US10659321
申请日：2003-09-11
申请人： David Levi , Mark Pickering , Lloyd Smith
发明人： David Levi , Mark Pickering , Lloyd Smith
IPC分类号： F24C15/32
CPC分类号： F24C15/322
摘要： A gas cooking appliance includes a combination radiant/convection cooking system mounted below a bottom wall portion of an oven cavity. The combination cooking system includes a gas burner in the form of a ring element having a central portion within which is positioned a convection fan. In addition to the convection fan, a forced air combustion fan supplies a combustion airflow to the gas burner. The oven preferably includes a glass panel arranged in a bottom wall portion of the oven cavity, above the gas burner. Arranged about the central opening are a plurality of vented openings for introducing the convective airflow into the oven cavity. The glass panel provides both a conductive heat surface transmitting at least a portion of the radiate heat energy into the oven cavity and a viewing surface allowing a consumer to view the operation of the gas burner.
摘要翻译：燃气烹饪器具包括安装在烤箱腔的底壁部分下方的辐射/对流烹调系统。组合烹饪系统包括具有中心部分的环形元件形式的气体燃烧器，在其中定位有对流风扇。除了对流风扇之外，强制空气燃烧风扇向燃气燃烧器供应燃烧气流。烘箱优选地包括布置在炉腔的底壁部分中的气体燃烧器上方的玻璃面板。围绕中心开口排列的是用于将对流气流引入烘箱腔的多个通气孔。玻璃面板提供将辐射热能的至少一部分传递到烤箱腔中的导电热表面和允许消费者观察气体燃烧器的操作的观察表面。

10. 发明授权

US06805462B1 Towable light tower and power plant 失效
标题翻译：牵引灯塔和发电厂
公开(公告)号：US06805462B1
公开(公告)日：2004-10-19
申请号：US10234011
申请日：2002-09-03
申请人： Lloyd Smith , Robert C. Dezan , Philip H. Dupuis
发明人： Lloyd Smith , Robert C. Dezan , Philip H. Dupuis
IPC分类号： B60Q100
CPC分类号： B60P3/14
摘要： A towable power plant is disclosed capable of supplying visible light for use at a worksite and at least on other power source. The power source may generate compressed air, electricity, or may pump water or a hydraulic fluid. The light tower may be coupled to the drawbar of an existing towable power plant.
摘要翻译：公开了一种能够提供在工地和至少其他电源上使用的可见光的可拖动发电厂。电源可能产生压缩空气，电力，或者可以泵送水或液压流体。光塔可以联接到现有可拖动发电厂的牵引杆。

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