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1. 发明授权

US5986079A Expression of pace in host cells and methods of use thereof 失效
公开(公告)号：US5986079A
公开(公告)日：1999-11-16
申请号：US480382
申请日：1995-06-07
申请人： Philip J. Barr , Anthony J. Brake , Randal J. Kaufman , Patricia Tekamp-Olson , Louise Wasley , Polly A. Wong
发明人： Philip J. Barr , Anthony J. Brake , Randal J. Kaufman , Patricia Tekamp-Olson , Louise Wasley , Polly A. Wong
IPC分类号： C12N5/10 , C07K14/755 , C12N9/50 , C12N9/64 , C12N15/09 , C12N15/57 , C12P21/02 , C12N15/79 , C12N15/85
CPC分类号： C12N9/6454 , C07K14/755
摘要： Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.

2. 发明授权

US5965425A Expression of pace in host cells and methods of use thereof 失效
标题翻译：宿主细胞中步调的表达及其使用方法
公开(公告)号：US5965425A
公开(公告)日：1999-10-12
申请号：US745880
申请日：1996-11-08
申请人： Philip J. Barr , Anthony J. Brake , Randal J. Kaufman , Patricia Tekamp-Olson , Louise Wasley , Polly A. Wong
发明人： Philip J. Barr , Anthony J. Brake , Randal J. Kaufman , Patricia Tekamp-Olson , Louise Wasley , Polly A. Wong
IPC分类号： C12N5/10 , C07K14/755 , C12N9/50 , C12N9/64 , C12N15/09 , C12N15/57 , C12P21/02 , C12N9/48 , C12N15/79
CPC分类号： C12N9/6454 , C07K14/755
摘要： Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.
摘要翻译：提供用于内肽酶生产的组合物和方法，以及提高加工异源前体多肽对成熟多肽（包括需要γ-羧化生物活性的蛋白质）的效率。这些组合物和方法利用重组PACE，一种特异于二元氨基酸位点的哺乳动物内肽酶。

3. 发明授权

US5460950A Expression of PACE in host cells and methods of use thereof 失效
标题翻译： PACE在宿主细胞中的表达及其使用方法
公开(公告)号：US5460950A
公开(公告)日：1995-10-24
申请号：US885972
申请日：1992-05-20
申请人： Philip J. Barr , Anthony J. Brake , Randal J. Kaufman , Louise Wasley , Patricia Tekamp-Olson , Polly A. Wong
发明人： Philip J. Barr , Anthony J. Brake , Randal J. Kaufman , Louise Wasley , Patricia Tekamp-Olson , Polly A. Wong
IPC分类号： C12N5/10 , C07K14/755 , C12N9/50 , C12N9/64 , C12N15/09 , C12N15/57 , C12P21/02 , C12N15/00 , C12N15/81 , C12N15/85
CPC分类号： C12N9/6454 , C07K14/755
摘要： Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.
摘要翻译：提供用于内肽酶生产的组合物和方法，以及提高加工异源前体多肽对成熟多肽（包括需要γ-羧化生物活性的蛋白质）的效率。这些组合物和方法利用重组PACE，一种特异于二元氨基酸位点的哺乳动物内肽酶。

4. 发明授权

US4880734A Eukaryotic regulatable transcription 失效
标题翻译：真核调控转录
公开(公告)号：US4880734A
公开(公告)日：1989-11-14
申请号：US868639
申请日：1986-05-29
申请人： Rae L. Burke , Steven Rosenberg , Jeffrey R. Shuster , Patricia Tekamp-Olson , Pablo D. T. Valenzuela , Philip J. Barr
发明人： Rae L. Burke , Steven Rosenberg , Jeffrey R. Shuster , Patricia Tekamp-Olson , Pablo D. T. Valenzuela , Philip J. Barr
IPC分类号： C07K14/02 , C07K14/81 , C12N9/02 , C12N15/62 , C12N15/81
CPC分类号： C07K14/005 , C07K14/8125 , C12N15/62 , C12N15/81 , C12N9/0089 , C07K2319/00 , C07K2319/02 , C07K2319/40 , C07K2319/75 , C12N2730/10122
摘要： Methods and compositions are provided for efficient expression of genes in unicellular microoorganisms, particularly yeast. The systems involve an expression system employing transcriptional initiation regions from glycolytic enzymes, particularly a chimeric expression system, having a first region providing for regulatable or constitutive expression, a second region providing for transcriptional initiation, where regions one and two are not found joined together in functional relationship in nature, and optionally a sequence providing for a secretory leader and processing signal, where the expression cassette will be joined to a gene which may be homologous or heterologous to the host. The expression cassette can be used on an extrachromosomal element or integrated into the host genome, whereby continuous expression can be achieved or inducible expression is obtained, by virtue of the presence or absence of an inducer. Constructions may be provided, where structural genes may be introduced in reading frame with the cassette to provide for expression of the natural peptide, a fused peptide, or a peptide precursor.The S. cerevisiae strain AB110 (pAB24/P. vivax 1-5 was deposited at the A.T.C.C. on May 9, 1986, and given Accession No. 20797.
摘要翻译：提供了用于在单细胞微生物，特别是酵母中有效表达基因的方法和组合物。该系统涉及使用来自糖酵解酶，特别是嵌合表达系统的转录起始区的表达系统，其具有提供可调节或组成型表达的第一区域，提供转录起始的第二区域，其中未发现第一和第二区域连接在一起功能性关系，以及任选地提供分泌前导序列和处理信号的序列，其中表达盒将与可与宿主同源或异源的基因连接。表达盒可以在染色体外元件上使用或整合到宿主基因组中，由此可以通过诱导剂的存在或不存在来实现连续表达或获得诱导型表达。可以提供构建体，其中结构基因可以在盒子的阅读框中引入以提供天然肽，融合肽或肽前体的表达。酿酒酵母菌株AB110（pAB24 / P.vivax 1-5）于1986年5月9日保藏于A.T.C.C.，保藏号为20797。

5. 发明授权

US09637763B2 Recombinant production systems for aromatic molecules 有权
公开(公告)号：US09637763B2
公开(公告)日：2017-05-02
申请号：US14128540
申请日：2012-06-22
申请人： Philip J. Barr
发明人： Philip J. Barr
IPC分类号： C12P7/22 , C12P7/00 , C12N1/00 , C12N15/00 , C12P21/06 , C12P19/34 , C07H21/04 , C12N9/88 , C12N9/10 , C12N9/12 , C12N15/52 , C12P17/04
CPC分类号： C12P7/22 , C12N9/1007 , C12N9/1288 , C12N9/88 , C12N15/52 , C12P7/00 , C12P17/04
摘要： The invention relates to the production of aromatic molecules in prokaryotic and eukaryotic hosts such as E. coli, yeasts, filamentous fungi, algae, microalgae, other plant cells.

6. 发明授权

US07316915B2 Bak binding protein, DNA encoding the protein, and methods of use thereof 有权
标题翻译： Bak结合蛋白，编码蛋白的DNA及其使用方法
公开(公告)号：US07316915B2
公开(公告)日：2008-01-08
申请号：US10189294
申请日：2002-07-01
申请人： Michael C. Kiefer , Paul A. Fitzpatrick , Helen L. Gibson , Philip J. Barr
发明人： Michael C. Kiefer , Paul A. Fitzpatrick , Helen L. Gibson , Philip J. Barr
IPC分类号： C12P21/06
CPC分类号： C07K14/47 , A61K38/00 , A61K48/00 , C07K2319/00 , G01N2333/47 , G01N2500/02 , G01N2510/00
摘要： The present invention provides polynucleotide sequences (bbp) encoding a Bak Binding Protein (BBP) and fragments thereof that bind to Bak. The invention also provides a BBP which binds to Bak. The invention also provides recombinant host cells containing polynucleotides encoding BBP. The invention further provides antibodies that specifically bind to BBP. The invention further provides methods for detecting agents such as drugs that alter the binding of a BBP with a Bak protein. The invention further provides methods for detecting the presence of bbp or BBP in a biological sample, and further provides methods for modulating the levels of BBP in a cell. This invention additionally encompasses novel peptides, designated the “BBP Binding Domains” and the respective nucleotides, designated “bbpbd-1” and “bbpbd-2” which are involved in the interaction between Bak and BBP.
摘要翻译：本发明提供编码Bak结合蛋白（BBP）的多核苷酸序列（bbp）及其与Bak结合的片段。本发明还提供了结合Bak的BBP。本发明还提供含有编码BBP的多核苷酸的重组宿主细胞。本发明还提供了特异性结合BBP的抗体。本发明还提供了用于检测诸如改变BBP与Bak蛋白的结合的药物的药物的方法。本发明还提供了用于检测生物样品中bbp或BBP的存在的方法，并且还提供了调节细胞中BBP水平的方法。本发明另外包括称为“BBP结合域”的新肽，以及涉及Bak和BBP之间的相互作用的“bbpbd-1”和“bbpbd-2”的相应核苷酸。

7. 发明授权

US06441135B1 Bak binding protein, DNA encoding the protein, and methods of use thereof 有权
标题翻译： Bak结合蛋白，编码蛋白的DNA及其使用方法
公开(公告)号：US06441135B1
公开(公告)日：2002-08-27
申请号：US09381488
申请日：2000-02-11
申请人： Michael C. Kiefer , Paul A. Fitzpatrick , Helen L. Gibson , Philip J. Barr
发明人： Michael C. Kiefer , Paul A. Fitzpatrick , Helen L. Gibson , Philip J. Barr
IPC分类号： C07K100
CPC分类号： C07K14/47 , A61K38/00 , A61K48/00 , C07K2319/00 , G01N2333/47 , G01N2500/02 , G01N2510/00
摘要： The present invention provides polynucleotide sequences (bbp) encoding a Bak Binding Protein (BBP) and fragments thereof that bind to Bak. The invention also provides a BBP which binds to Bak. The invention also provides recombinant host cells containing polynucleotides encoding BBP. The invention further provides antibodies that specifically bind to BBP. The invention further provides methods for detecting agents such as drugs that alter the binding of a BBP with a Bak protein. The invention neither provides methods for detecting the presence of bbp or BBP in a biological sample, and further provides methods for modulating the levels of BBP in a cell. This invention additionally encompasses novel peptides, designated the “BBP Binding Domains” and the respective nucleotides, designated “bbpbd-1” and “bbpbd-2” which arc involved in the interaction between Bak and BBP.
摘要翻译：本发明提供编码Bak结合蛋白（BBP）的多核苷酸序列（bbp）及其与Bak结合的片段。本发明还提供了结合Bak的BBP。本发明还提供含有编码BBP的多核苷酸的重组宿主细胞。本发明还提供了特异性结合BBP的抗体。本发明还提供了用于检测诸如改变BBP与Bak蛋白的结合的药物的药物的方法。本发明既没有提供用于检测生物样品中bbp或BBP的存在的方法，并且还提供了调节细胞中BBP水平的方法。本发明还包括命名为“BBP结合域”的新肽，以及涉及Bak和BBP之间相互作用的“bbpbd-1”和“bbpbd-2”的相应核苷酸。

8. 发明授权

US5863756A Compositions and methods for PACE 4 and 4.1 gene and polypeptides in cells 失效
公开(公告)号：US5863756A
公开(公告)日：1999-01-26
申请号：US284941
申请日：1994-08-02
申请人： Philip J. Barr , Michael C. Kiefer
发明人： Philip J. Barr , Michael C. Kiefer
IPC分类号： C12N15/09 , A61K38/00 , A61K38/55 , C07H21/04 , C12N1/21 , C12N5/10 , C12N9/64 , C12N15/57 , C12R1/00 , C12R1/91 , C12N15/00
CPC分类号： C12N9/6454 , A61K38/00 , C07K2319/00
摘要： Compositions and methods are provided for endopeptidases and their production, and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides. These compositions and methods utilize recombinant PACE 4 and 4.1, mammalian endopeptidases that are specific for dibasic amino acid sites. Therapeutic compositions and methods employing PACE 4 or 4.1 or their inhibitors are also provided.

9. 发明授权

US5770443A Apoptosis-modulating proteins, DNA encoding the proteins and methods of use thereof 失效
公开(公告)号：US5770443A
公开(公告)日：1998-06-23
申请号：US471058
申请日：1995-06-06
申请人： Michael C. Kiefer , Philip J. Barr
发明人： Michael C. Kiefer , Philip J. Barr
IPC分类号： A01K67/027 , A61K38/00 , C07H21/04 , C07K14/47 , C07K16/18 , C12N5/10 , C12N15/09 , C12N15/13 , C12N15/85 , C12P21/02 , C12P21/08 , C12Q1/68 , C12R1/91 , G01N33/53 , C12N15/63
CPC分类号： C12N15/8509 , C07K14/4747 , C12Q1/6876 , A01K2217/05 , A01K2227/105 , A01K2267/025 , A01K2267/03 , A61K38/00 , C12Q2600/158
摘要： The present invention provides a novel family of apoptosis-modulating proteins. Nucleotide and amino acid residue sequences and methods of use thereof are also provided.

10. 发明授权

US5620867A Bone morphogenetic protein expression and DNA 失效
标题翻译：骨形态发生蛋白表达和DNA
公开(公告)号：US5620867A
公开(公告)日：1997-04-15
申请号：US142847
申请日：1993-10-26
申请人： Michael C. Kiefer , Frank R. Masiarz , Philip J. Barr
发明人： Michael C. Kiefer , Frank R. Masiarz , Philip J. Barr
IPC分类号： C12P21/00 , A61K38/00 , A61P43/00 , C07K14/00 , C07K14/51 , C07K16/00 , C07K16/22 , C12N15/12 , C12N15/16 , C12P21/08 , C12R1/865 , C12N15/18 , C12N15/11 , C12N5/10 , C12N15/63
CPC分类号： C07K16/22 , C07K14/51 , A61K38/00 , C07K2319/02
摘要： The purification and cloning of bone morphogenetic proteins are disclosed, as well as production of BMP and its analogs thereof by recombinant DNA techniques. Pharmaceutical compositions comprising BMP and the use of such compositions are also disclosed.
摘要翻译：公开了骨形态发生蛋白的纯化和克隆，以及通过重组DNA技术生产BMP及其类似物。还公开了包含BMP的药物组合物和这种组合物的用途。

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