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2. 发明授权

US5798253A Method of culturing micro-organisms under a mutagenic influence 失效
标题翻译：在诱变影响下培养微生物的方法
公开(公告)号：US5798253A
公开(公告)日：1998-08-25
申请号：US704749
申请日：1996-10-29
申请人： Peter Bartholmes , Michael Kaufmann , Thomas Schwarz
发明人： Peter Bartholmes , Michael Kaufmann , Thomas Schwarz
IPC分类号： C12M1/00 , C02F3/34 , C12N1/26 , C12N1/36 , C12N1/00 , C12N1/20
CPC分类号： C12N1/26 , C02F3/34 , C12N1/36 , Y10S435/813 , Y10S435/819 , Y10S435/821 , Y10S435/822
摘要： An accelerated micro-organism culture method is for decomposing harmful substances. For this purpose, the cultivation, mutagenic treatment and stabilization of the micro-organisms are carried out simultaneously in a mutation fermenter containing a nutrient-rich basic medium (basic medium 1); selection is carried out in a selection fermenter containing a basic medium which is low in nutrients and is enriched with the harmful substance (basic medium 2); small amounts of biomass released from the nutrient-rich basic medium (basic medium 1) of the mutation fermenter are continuously removed from the mutation fermenter and transferred to the selection fermenter. Small amounts of biomass released from the basic medium which is low in nutrients (basic medium 2) of the selection fermenter are removed from the selection fermenter and transferred to the mutation fermenter. The entire process is carried out continuously in alternating selection and mutation phases, in the manner of a repeated cycle. The mutation fermenter and the selection fermenter are associated with computerized control circuits by means of which the fermentation parameters can be adjusted independently of one another in both fermenters.
摘要翻译： PCT No.PCT / EP95 / 00752 Sec。 371日期1996年10月29日第 102（e）日期1996年10月29日PCT 1995年3月1日PCT PCT。出版物WO95 / 23845 日期1995年9月8日加速微生物培养方法是分解有害物质。为此，在含有营养丰富的碱性培养基（碱性培养基1）的突变发酵罐中同时进行微生物的培养，诱变处理和稳定化。选择在含有营养成分低且富含有害物质（碱性培养基2）的碱性培养基的选择发酵器中进行）从突变发酵罐的营养丰富的碱性培养基（碱性培养基1）中释放的少量生物量从突变发酵罐中连续移出并转移到选择发酵罐中。从选择发酵罐的营养物质（碱性培养基2）中低碱性培养基中释放的少量生物质从选择发酵罐中移出并转移到突变发酵罐中。整个过程以交替的选择和突变阶段以重复循环的方式连续进行。突变发酵罐和选择发酵罐与计算机控制回路相关联，通过这些电路可以在发酵罐中彼此独立地调节发酵参数。

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