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1. 发明授权

US09500589B2 Sample holding carrier and fluorescence detection device using same 有权
标题翻译：样品保持载体和使用其的荧光检测装置
公开(公告)号：US09500589B2
公开(公告)日：2016-11-22
申请号：US14514048
申请日：2014-10-14
申请人： PANASONIC CORPORATION , SANYO ELECTRIC CO., LTD.
发明人： Morio Nakatani , Masaya Nakatani , Yoshiyuki Matsumura , Kenji Nagatomi , Akio Oki
IPC分类号： G01N21/64 , G01N21/27
CPC分类号： G01N21/6452 , G01N21/274 , G01N21/6428 , G01N21/645 , G01N21/6486 , G01N2021/6439
摘要： Disclosed is a sample holding carrier allowing samples to be measured accurately, and a fluorescence detection device for use with the sample holding carrier. A biosensor substrate includes a base substrate, a plurality of wells formed on a first surface side of the base substrate; and grooves formed on the first surface side of the base substrate separately from the wells and generating fluorescence under exposure to excitation light. The fluorescence detection device applies excitation light to the grooves, thereby figuring out the level of the fluorescence to be detected from the biosensor substrate. As a result, the fluorescence detection device can amplify the detection signals of the fluorescence generated when the excitation light is applied to the wells to an appropriate level, thereby accurately detecting the fluorescence generated in the samples.
摘要翻译：公开了一种允许准确测量样品的样品保持载体和用于样品保持载体的荧光检测装置。生物传感器基板包括基底基板，形成在基底基板的第一表面侧上的多个阱; 以及与所述孔分开形成在所述基底基板的第一表面侧上的凹槽，并且在暴露于激发光的情况下产生荧光。荧光检测装置将激发光施加到沟槽，从而从生物传感器基板上确定要检测的荧光的水平。结果，荧光检测装置可以将激发光施加到阱上产生的荧光的检测信号放大到适当的水平，从而准确地检测样品中产生的荧光。

2. 发明授权

US09128056B2 Sample holding carrier, and fluorescence detection system and fluorescence detection device that use same 有权
标题翻译：样品保持载体，荧光检测系统和荧光检测装置使用相同
公开(公告)号：US09128056B2
公开(公告)日：2015-09-08
申请号：US14498877
申请日：2014-09-26
申请人： PANASONIC CORPORATION , SANYO Electric Co., Ltd.
发明人： Yoshiyuki Matsumura , Kenji Nagatomi , Masaya Nakatani , Akio Oki , Morio Nakatani , Takuya Hayashi , Masaaki Hayama
IPC分类号： G01J1/58 , G01N21/64 , G01N21/03 , G01N35/00 , G11B7/00 , G11B7/09 , B01L3/00
CPC分类号： G01N21/645 , B01L3/5085 , B01L2300/024 , B01L2300/0654 , B01L2300/0803 , B01L2300/0806 , B01L2300/0819 , B01L2300/0893 , B01L2300/168 , G01N21/03 , G01N21/6447 , G01N21/6452 , G01N35/00069 , G01N2021/6463 , G01N2021/6482 , G01N2201/105 , G11B7/00 , G11B7/09
摘要： The present invention provides a sample holding carrier that can accurately measure a sample with a simple configuration, and a fluorescence detection system and device that use the same. Biosensor substrate includes: base substrate on which excitation light is incident from a lower surface; reflecting film that is arranged on an upper surface of base substrate to partially reflect the excitation light; and plural wells that are arranged on an upper surface side of reflecting film and have bottom surface portions. The excitation light is converged to be incident on base substrate. Distance from reflecting surface that is of a boundary between reflecting film and base substrate to bottom surface portion of well is less than or equal to a focal depth of the excitation light. Therefore, the sample accommodated in bottom surface portion of well can surely and efficiently be irradiated with the excitation light, and accurately be measured.
摘要翻译：本发明提供一种能够以简单的结构准确测量样品的样品保持载体和使用其的荧光检测系统和装置。生物传感器基板包括：激发光从其下表面入射的基底基板; 反射膜，其布置在基底基板的上表面上以部分地反射激发光; 以及布置在反射膜的上表面侧并具有底表面部分的多个阱。激发光会聚到入射到基底基板上。与反射膜和基底之间的边界的反射面与孔的底面部的距离小于或等于激发光的焦深。因此，能够可靠且有效地利用激发光照射容纳在井底部的样品，并且可以精确地测量。

3. 发明授权

US09046490B2 Fluorescence detection device 有权
标题翻译：荧光检测装置
公开(公告)号：US09046490B2
公开(公告)日：2015-06-02
申请号：US14498596
申请日：2014-09-26
申请人： PANASONIC CORPORATION , SANYO Electric Co., Ltd.
发明人： Kenji Nagatomi , Masaya Nakatani
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , G01N21/645 , G01N21/6452 , G01N2021/6463
摘要： To provide a fluorescence detection device that can effectively remove autofluorescence using a simple configuration. A fluorescence detection device comprises: semiconductor laser for emitting excitation light; an objective lens for converging excitation light onto a sample on a biosensor substrate; an anamorphic lens which introduces astigmatism to fluorescence that is from the biosensor substrate and incident on the objective lens and that passed through the objective lens; a spectral element for separating the fluorescence into a plurality of light rays; and a fluorescence detector for receiving the light rays separated by the spectral element. On the light receiving surface of the fluorescence detector, the spectral element splits the fluorescence so that the fluorescence generated at the sample is separated from the fluorescence generated at a specific depth position other than the sample position.
摘要翻译：提供可以使用简单配置有效去除自体荧光的荧光检测装置。荧光检测装置包括：用于发射激发光的半导体激光器; 用于将激发光会聚到生物传感器基板上的样品上的物镜; 一种变形透镜，其对来自生物传感器基板的荧光引入散光，并入射到物镜上并穿过物镜; 用于将荧光分离成多个光线的光谱元件; 以及用于接收由光谱元件分离的光线的荧光检测器。在荧光检测器的光接收表面上，光谱元件分离荧光，使得在样品处产生的荧光与在样品位置以外的特定深度位置处产生的荧光分离。

4. 发明申请

US20150014552A1 FLUORESCENCE DETECTION DEVICE 有权
标题翻译：荧光检测装置
公开(公告)号：US20150014552A1
公开(公告)日：2015-01-15
申请号：US14498596
申请日：2014-09-26
申请人： PANASONIC CORPORATION , SANYO Electric Co., Ltd.
发明人： Kenji Nagatomi , Masaya Nakatani
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , G01N21/645 , G01N21/6452 , G01N2021/6463
摘要： To provide a fluorescence detection device that can effectively remove autofluorescence using a simple configuration. A fluorescence detection device comprises: semiconductor laser for emitting excitation light; an objective lens for converging excitation light onto a sample on a biosensor substrate; an anamorphic lens which introduces astigmatism to fluorescence that is from the biosensor substrate and incident on the objective lens and that passed through the objective lens; a spectral element for separating the fluorescence into a plurality of light rays; and a fluorescence detector for receiving the light rays separated by the spectral element. On the light receiving surface of the fluorescence detector, the spectral element splits the fluorescence so that the fluorescence generated at the sample is separated from the fluorescence generated at a specific depth position other than the sample position.
摘要翻译：提供可以使用简单配置有效去除自体荧光的荧光检测装置。荧光检测装置包括：用于发射激发光的半导体激光器; 用于将激发光会聚到生物传感器基板上的样品上的物镜; 变形透镜，其对来自生物传感器基板的荧光引入散光，并入射到物镜上并穿过物镜; 用于将荧光分离成多个光线的光谱元件; 以及用于接收由光谱元件分离的光线的荧光检测器。在荧光检测器的光接收表面上，光谱元件分离荧光，使得在样品处产生的荧光与在样品位置以外的特定深度位置处产生的荧光分离。

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