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1. 发明授权

US06350934B1 Nucleic acid encoding delta-9 desaturase 失效
标题翻译：编码Δ-9去饱和酶的核酸
公开(公告)号：US06350934B1
公开(公告)日：2002-02-26
申请号：US08679645
申请日：1996-07-12
申请人： Michael G. Zwick , Brent E. Edington , James A. McSwiggen , Patricia Ann Owens Merlo , Lining Guo , Thomas A. Skokut , Scott A. Young , Otto Folkerts , Donald J. Merlo
发明人： Michael G. Zwick , Brent E. Edington , James A. McSwiggen , Patricia Ann Owens Merlo , Lining Guo , Thomas A. Skokut , Scott A. Young , Otto Folkerts , Donald J. Merlo
IPC分类号： C12N504
CPC分类号： C12N15/113 , C12N9/0083 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/8255 , C12N2310/12 , C12N2310/121 , C12N2310/122
摘要： The present invention relates to nucleic acid molecules encoding delta 9 desaturase gene, and expression vectors, plant cells, and transgenic plants expressing delta 9 desaturase nucleic acid. The nucleic acid molecules of the present invention can be used, for example, to decrease delta 9 desaturase activity in plant cells, resulting in decreased unsaturated fatty acid production.
摘要翻译：本发明涉及编码Δ9去饱和酶基因的核酸分子和表达载体，植物细胞和表达Δ9去饱和酶核酸的转基因植物。本发明的核酸分子可以用于例如降低植物细胞中δ9去饱和酶的活性，导致不饱和脂肪酸的产生降低。

2. 发明授权

US06495738B1 Modification of fatty acid composition in plants by expression of a fungal acyl-CoA desaturase 有权
标题翻译：通过真菌酰基辅酶A去饱和酶的表达修饰植物中的脂肪酸组成
公开(公告)号：US06495738B1
公开(公告)日：2002-12-17
申请号：US09280428
申请日：1999-03-29
申请人： Otto Folkerts , Donald J. Merlo
发明人： Otto Folkerts , Donald J. Merlo
IPC分类号： A01H500
CPC分类号： C12N9/0083 , C12N15/8247
摘要： Genes-encoding a delta-9 CoA desaturase from Aspergillus nidulans have been isolated. The proteins encoded by genes, when expressed in a plant, can alter the saturate levels of the oil.
摘要翻译：已经分离了从构巢曲霉编码Δ-9 CoA去饱和酶的基因。由基因编码的蛋白质，当在植物中表达时，可以改变油的饱和水平。

3. 发明授权

US6166302A Modified Bacillus thuringiensis gene for lepidopteran control in plants 失效
标题翻译：改良的苏云金芽孢杆菌基因在植物中的鳞翅目控制
公开(公告)号：US6166302A
公开(公告)日：2000-12-26
申请号：US729601
申请日：1996-10-11
申请人： Donald J. Merlo , Otto Folkerts
发明人： Donald J. Merlo , Otto Folkerts
IPC分类号： C12N15/09 , A01H5/00 , A01H5/10 , C07K14/325 , C12N15/11 , C12N15/32 , C12N15/67 , C12N15/82
CPC分类号： C12N15/8216 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Synthetic DNA sequences which are optimized for expression in plants, particularly maize, and which encode a Bacillus thuringiensis protein that is toxic to specific insects are provided, along with methods for the engineering of any synthetic insecticidal gene in maize.
摘要翻译：提供了优化用于在植物，特别是玉米中表达并且编码对特定昆虫有毒的苏云金芽孢杆菌蛋白质的合成DNA序列，以及在玉米中任何合成杀虫基因工程的方法。

4. 发明授权

US09127289B2 Herbicide resistance genes 有权
标题翻译：除草剂抗性基因
公开(公告)号：US09127289B2
公开(公告)日：2015-09-08
申请号：US12951813
申请日：2010-11-22
申请人： Terry R. Wright , Justin M. Lira , Donald J. Merlo , Nicole L. Arnold
发明人： Terry R. Wright , Justin M. Lira , Donald J. Merlo , Nicole L. Arnold
IPC分类号： C12N15/82 , C12N9/02
CPC分类号： C12N15/8274 , C12N9/0069 , C12N15/8209
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
摘要翻译：本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和改善除草剂抗性管理方案。更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。本发明还包括控制杂草的相关方法。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

5. 发明申请

US20110107455A1 Novel Selectable Marker Genes 审中-公开
标题翻译：新型可选标记基因
公开(公告)号：US20110107455A1
公开(公告)日：2011-05-05
申请号：US12517906
申请日：2007-12-07
申请人： Justin M. Lira , Terry R. Wright , Sean M. Russell , Donald J. Merlo , Steven Robert Webb , Nicole L. Arnold , Andrew E. Robinson , Kelley A. Smith
发明人： Justin M. Lira , Terry R. Wright , Sean M. Russell , Donald J. Merlo , Steven Robert Webb , Nicole L. Arnold , Andrew E. Robinson , Kelley A. Smith
IPC分类号： C12N15/82 , C12N5/10 , C12N15/63 , C12Q1/04 , A01H5/10 , A01H1/00 , C12N9/10 , C12N1/21 , A01N57/18 , C12Q1/68 , A01H5/00
摘要： The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.
摘要翻译：本发明涉及本文称为DSM-2的新基因。该基因在天竺葵A3中鉴定。 DSM-2蛋白与PAT和BAR密切相关。本发明还提供编码DSM-2蛋白质的植物优化基因，DSM-2可用作转基因性状，以将植物和植物细胞中的耐受性赋予除草剂草铵膦和双丙二酸。主题基因的一个优选用途是选择标记。在细菌系统中使用该基因作为选择标记可以提高植物转化的效率。使用DSM-2作为唯一选择标记，在克隆过程中不需要额外的药用抗生素标记（如氨苄青霉素抗性）。根据本发明，各种其他用途也是可能的。

6. 发明申请

US20090093366A1 Novel Herbicide Resistance Genes 有权
标题翻译：新型除草剂抗性基因
公开(公告)号：US20090093366A1
公开(公告)日：2009-04-09
申请号：US11587893
申请日：2005-05-02
申请人： Terry R. Wright , Justin M. Lira , Donald J. Merlo , Nicole L. Arnold
发明人： Terry R. Wright , Justin M. Lira , Donald J. Merlo , Nicole L. Arnold
IPC分类号： A01N39/02 , C12N5/10 , A01H5/00 , A01N37/00 , C07H21/04 , C12Q1/68
CPC分类号： C12N15/8274 , C12N9/0069 , C12N15/8209
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
摘要翻译：本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和改善除草剂抗性管理方案。更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。本发明还包括控制杂草的相关方法。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

7. 发明授权

US6143526A Biosynthetic genes for spinosyn insecticide production 失效
标题翻译：多杀菌素杀虫剂生产的生物合成基因
公开(公告)号：US6143526A
公开(公告)日：2000-11-07
申请号：US036987
申请日：1998-03-09
申请人： Richard H. Baltz , M. Christine Broughton , Kathryn P. Crawford , Krishnamurthy Madduri , Donald J. Merlo , Patti J. Treadway , Jan R. Turner , Clive Waldron
发明人： Richard H. Baltz , M. Christine Broughton , Kathryn P. Crawford , Krishnamurthy Madduri , Donald J. Merlo , Patti J. Treadway , Jan R. Turner , Clive Waldron
IPC分类号： C12N15/09 , C07K14/195 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/52 , C12P19/62 , C12Q1/68 , C07H21/04 , C12N1/14 , C12N9/00
CPC分类号： C12N15/52 , C07K14/195 , C12P19/62
摘要： Spinosyn biosynthetic genes, spinosyn producing microorganisms transformed with the biosynthetic genes, methods using the biosynthetic genes to increase production of spinosyn insecticidal macrolides, and methods using the genes or fragments thereof to change the products produced by spinosyn-producing microorganisms.
摘要翻译： Spinosyn生物合成基因，用生物合成基因转化的多杀菌素微生物，使用生物合成基因增加多杀菌素杀虫大环内酯类的生产的方法，以及使用其基因或片段改变由产生多杀菌素的微生物产生的产物的方法。

8. 发明授权

US5567862A Synthetic insecticidal crystal protein gene 失效
公开(公告)号：US5567862A
公开(公告)日：1996-10-22
申请号：US369839
申请日：1995-01-06
申请人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
发明人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
IPC分类号： C07K14/325 , C12N15/32 , C12N15/82 , A01H4/00 , C12N5/14
CPC分类号： C07K14/325 , C12N15/8216 , C12N15/8286
摘要： Synthetic Bacillus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.

9. 发明授权

US09617551B2 Method of increasing plant transformation frequency using modified strains of Agrobacteria 有权
公开(公告)号：US09617551B2
公开(公告)日：2017-04-11
申请号：US13812469
申请日：2011-07-29
申请人： Donald J. Merlo , Sean M. Russell , Diane M. Retallack , Aaron T. Woosley , Thomas Meade , Kenneth Narva
发明人： Donald J. Merlo , Sean M. Russell , Diane M. Retallack , Aaron T. Woosley , Thomas Meade , Kenneth Narva
IPC分类号： C12N15/82 , C12N15/74
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

10. 发明授权

US09434773B2 Plants having stress related traits and methods for making the same 有权
标题翻译：具有压力相关性状的植物及其制作方法
公开(公告)号：US09434773B2
公开(公告)日：2016-09-06
申请号：US13617207
申请日：2012-09-14
申请人： Avutu S. Reddy , Sun Yuejin , Donald J. Merlo , Weiting W. Ni , Dayakar Pareddy , Zhifang Gao , Gregory A. Bradfisch
发明人： Avutu S. Reddy , Sun Yuejin , Donald J. Merlo , Weiting W. Ni , Dayakar Pareddy , Zhifang Gao , Gregory A. Bradfisch
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N9/00 , C07K14/415
CPC分类号： C07K14/415 , C12N9/93 , C12N15/8273 , C12Y603/02019
摘要： This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.
摘要翻译：本发明一般涉及通过增加或产生植物中RING蛋白的一种或多种活性而与相应的非转化野生型植物细胞相比，优选在瞬时和重复的非生物胁迫条件下具有增加的产量的植物细胞。

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