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1. 发明授权

US5567862A Synthetic insecticidal crystal protein gene 失效
公开(公告)号：US5567862A
公开(公告)日：1996-10-22
申请号：US369839
申请日：1995-01-06
申请人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
发明人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
IPC分类号： C07K14/325 , C12N15/32 , C12N15/82 , A01H4/00 , C12N5/14
CPC分类号： C07K14/325 , C12N15/8216 , C12N15/8286
摘要： Synthetic Bacillus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.

2. 发明授权

US5567600A Synthetic insecticidal crystal protein gene 失效
标题翻译：合成杀虫晶体蛋白基因
公开(公告)号：US5567600A
公开(公告)日：1996-10-22
申请号：US369835
申请日：1995-01-06
申请人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
发明人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
IPC分类号： C07K14/325 , C12N15/82 , C12N15/32
CPC分类号： C12N15/8216 , C07K14/325 , C12N15/8286
摘要： Synthetic Baccilus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.
摘要翻译：提供了设计用于在高于天然存在的Bt基因的植物中表达的合成的苏木精苏木精毒素基因。这些基因使用在高度表达的单子叶植物或双子叶植物蛋白质中优选的密码子。

3. 发明授权

US5380831A Synthetic insecticidal crystal protein gene 失效
标题翻译：合成杀虫晶体蛋白基因
公开(公告)号：US5380831A
公开(公告)日：1995-01-10
申请号：US57191
申请日：1993-05-03
申请人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
发明人： Michael J. Adang , Thomas A. Rocheleau , Donald J. Merlo , Elizabeth E. Murray
IPC分类号： C07K14/325 , C12N15/32 , C12N15/82
CPC分类号： C07K14/325 , C12N15/8216 , C12N15/8286
摘要： Synthetic Bacillus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.
摘要翻译：提供了设计为在高于天然存在的Bt基因的植物中表达的合成苏云金芽孢杆菌毒素基因。这些基因使用在高度表达的单子叶植物或双子叶植物蛋白质中优选的密码子。

4. 发明授权

US6015891A Synthetic insecticidal crystal protein gene having a modified frequency of codon usage 失效
标题翻译：具有修饰密码子使用频率的合成杀虫晶体蛋白基因
公开(公告)号：US6015891A
公开(公告)日：2000-01-18
申请号：US705438
申请日：1996-08-29
申请人： Michael J. Adang , Elizabeth E. Murray
发明人： Michael J. Adang , Elizabeth E. Murray
IPC分类号： C07K14/325 , C12N15/82 , C12N15/00 , C12N15/32
CPC分类号： C07K14/325 , C12N15/8286
摘要： Synthetic Baccilus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.
摘要翻译：提供了设计用于在高于天然存在的Bt基因的植物中表达的合成的苏木精苏木精毒素基因。这些基因使用在高度表达的单子叶植物或双子叶植物蛋白质中优选的密码子。

5. 发明授权

US6013523A Transgenic plants comprising a synthetic insecticidal crystal protein gene having a modified frequency of codon usage 失效
标题翻译：包含具有修饰频率密码子使用的合成杀虫晶体蛋白基因的转基因植物
公开(公告)号：US6013523A
公开(公告)日：2000-01-11
申请号：US704966
申请日：1996-08-29
申请人： Michael J. Adang , Elizabeth E. Murray
发明人： Michael J. Adang , Elizabeth E. Murray
IPC分类号： A01H5/00 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/32 , C12N15/82 , C12P21/02 , C12R1/07 , C12N5/14
CPC分类号： C07K14/325 , C12N15/8286
摘要： Synthetic Baccilus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.
摘要翻译：提供了设计用于在高于天然存在的Bt基因的植物中表达的合成的苏木精苏木精毒素基因。这些基因使用在高度表达的单子叶植物或双子叶植物蛋白质中优选的密码子。

6. 发明申请

US20110112012A1 Novel Protein for Binding Bacillus Thuringiensis Cry Toxins and Fragments of Cadherins for Enhancing Cry Toxicity Against Dipterans 审中-公开
标题翻译：用于结合苏云金芽孢杆菌的新型蛋白质用于增强对Dipterans的Cry毒性的卡介素的毒素和片段
公开(公告)号：US20110112012A1
公开(公告)日：2011-05-12
申请号：US12936027
申请日：2009-04-03
申请人： Michael J. Adang , Gang Hua , Mohd Amir Fursan Abdullah , Rui Zhang
发明人： Michael J. Adang , Gang Hua , Mohd Amir Fursan Abdullah , Rui Zhang
IPC分类号： A01N37/18 , A01P7/04 , C07K14/435 , A01H5/00
CPC分类号： A01N63/02 , C07K14/43563 , C07K14/705 , C12N15/8286 , Y02A40/162 , A01N2300/00
摘要： The subject invention relates in part to a novel protein for binding Bacillus thuringiensis Cry toxins, and fragments of cadherins for enhancing Cry toxicity against dipterans. The subject invention also relates in part to the discovery that fragments of a midgut cadherin from a dipteran insect synergize Cry proteins that are active against dipterans. Thus, the subject invention includes the use of fragments of cadherin ectodomains for controlling dipterans. Such fragments (that bind Crys) can be administered to a dipteran insect for ingestion. In some preferred embodiments, the source cadherin is a dipteran cadherin. Also in some preferred embodiments, the fragment is administered with a Cry protein that is active against a dipteran. Variants of the fragments of naturally occurring cadherins are included within the scope of the subject invention.
摘要翻译：本发明部分涉及用于结合苏云金芽孢杆菌Cry毒素的新型蛋白质和用于增强对dip子素的Cry毒性的钙粘蛋白片段。本发明还部分涉及这样一个发现，即来自二极虫昆虫的中肠钙粘蛋白的片段与对dip虫有活性的Cry蛋白协同作用。因此，本发明包括使用钙粘蛋白胞外域的片段来控制dip子。可以将这样的片段（结合Crys）施用于用于摄取的dip虫。在一些优选的实施方案中，源钙粘蛋白是二极体钙粘蛋白。同样在一些优选的实施方案中，所述片段与对dipteran有活性的Cry蛋白施用。天然存在的钙粘素的片段的变体包括在本发明的范围内。

7. 发明授权

US07345229B1 Insect resistant cotton plants 失效
标题翻译：抗虫棉花植物
公开(公告)号：US07345229B1
公开(公告)日：2008-03-18
申请号：US08478153
申请日：1995-06-07
申请人： Michael J. Adang , John D. Kemp , Ebrahim Firoozabady
发明人： Michael J. Adang , John D. Kemp , Ebrahim Firoozabady
IPC分类号： C12N15/82 , A01H5/00 , C12N15/32
CPC分类号： C07K14/325 , C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： A method for expressing insecticidal protein structural genes in cotton plant genomes is provided. In the preferred embodiments this invention comprises placing a structural gene for the Bacillus thuringiensis crystal protein under control of a plant or a T-DNA promoter and ahead of a polyadenylation site followed by insertion of said promoter/structural gene combination into a plant genome by utilizing an Agrobacterium tumefaciens Ti plasmid-based transformation system. The modified Ti plasmid is then used to transform recipient plant cells. Also provided are the plants and tissues produced by this method and bacterial strains, plasmids, and vectors useful for execution of this invention.
摘要翻译：提供了一种在棉花植物基因组中表达杀虫蛋白结构基因的方法。在优选实施方案中，本发明包括将苏云金芽孢杆菌晶体蛋白质的结构基因置于植物或T-DNA启动子的控制下，并在多腺苷酸化位点之前，然后通过利用所述启动子/结构基因组合插入植物基因组一种根癌土壤杆菌Ti质粒转化系统。然后将修饰的Ti质粒用于转化受体植物细胞。还提供了通过该方法产生的植物和组织以及用于实施本发明的细菌菌株，质粒和载体。

8. 发明申请

US20110183896A1 ENHANCEMENT OF BACILLUS THURINGIENSIS CRY PROTEIN TOXICITIES TO COLEOPTERANS, AND NOVEL INSECT CADHERIN FRAGMENTS 有权
标题翻译：咖啡因增强蛋白质蛋白质对蛋鸡的毒性和新型内脏蛋白质片段
公开(公告)号：US20110183896A1
公开(公告)日：2011-07-28
申请号：US12672974
申请日：2008-08-11
申请人： Michael J. Adang , Mohd Amir Fursan Abdullah
发明人： Michael J. Adang , Mohd Amir Fursan Abdullah
IPC分类号： A01N37/18 , C07K14/325 , A01P7/04 , A01P15/00
CPC分类号： A01N63/00 , A01N63/02 , A01N2300/00
摘要： The subject invention relates in part to the discovery that a fragment from a cadherin of the western corn rootworm enhances Cry3 toxicity to larvae of naturally susceptible species. The subject invention also relates in part to the discovery that a cadherin fragment from a beetle enhances Cry3Aa and Cry3Bb toxicity to coleopteran larvae, particularly those in the family Chrysomelidae. Such cadherin fragments are referred to as Bt Boosters (BtBs). The subject invention can be extended to the use of BtBs with other coleopteran-toxic Cry proteins for controlling a wide range of coleopterans.
摘要翻译：本发明部分地涉及以下发现：玉米根虫西瓜的钙粘蛋白的片段增强对天然易感物种的幼虫的Cry3毒性。本发明还部分涉及来自甲虫的钙粘着蛋白片段增强Cry3Aa和Cry3Bb对鞘翅目幼虫特别是家蝇科的鞘氨酯幼虫毒性的发现。这种钙粘蛋白片段被称为Bt Boosters（BtBosts）（BtBs）。本发明可以扩展到使用具有其它鞘翅目昆虫毒素Cry蛋白的BtB来控制广泛的鞘翅目。

9. 发明授权

US06251656B1 Insecticidal protein fragments 失效
标题翻译：杀虫蛋白片段
公开(公告)号：US06251656B1
公开(公告)日：2001-06-26
申请号：US09422833
申请日：1999-10-21
申请人： Michael J. Adang
发明人： Michael J. Adang
IPC分类号： C12N120
CPC分类号： C12N15/8216 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The Bacillus thuringiensis var. kurstaki HD-73 crystal protein gene was cloned into pBR322. E. coli cells harboring this recombinant plasmid produced a 130 kD protoxin that was toxic to Manduca sexta (tobacco hornworm) larvae. Plasmids having the 3′-end of the protoxin gene deleted where also constructed. E. coli cells harboring these deleted plasmids produced an active, soluble 68 kD toxin, provided that the 3′-deletion had not removed sequences encoding the 68 kD toxin. The invention provides methods to produce 68 kD toxin protein by constructing partial protoxin genes encoding the toxin followed by expression of the genes in living cells. Useful plasmids and cells are also provided.
摘要翻译：苏云金芽孢杆菌变种将kurstaki HD-73晶体蛋白基因克隆到pBR322中。含有该重组质粒的大肠杆菌细胞产生对Manduca sexta（烟草蛔虫）幼虫有毒的130kD原毒素。具有前毒素基因3'末端的质粒也被删除。携带这些缺失质粒的大肠杆菌细胞产生活性的可溶性68kD毒素，条件是3'缺失未除去编码68kD毒素的序列。本发明提供了通过构建编码毒素的部分原毒素基因，然后在活细胞中表达基因来产生68kD毒素蛋白质的方法。还提供了有用的质粒和细胞。

10. 发明授权

US08802389B2 Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods 有权
标题翻译：鉴定涉及抗Cry1毒素的毒素结合蛋白，以及相关筛选方法
公开(公告)号：US08802389B2
公开(公告)日：2014-08-12
申请号：US13274646
申请日：2011-10-17
申请人： Michael J. Adang , Rebecca McNall , Juan Luis Jurat Fuentes
发明人： Michael J. Adang , Rebecca McNall , Juan Luis Jurat Fuentes
IPC分类号： A01N25/00 , C12N1/20 , C12Q1/00 , C12Q1/02 , C12Q1/42 , G01N33/53 , G01N33/554 , G01N33/567 , G01N33/569
CPC分类号： G01N33/573 , C12Q1/42 , G01N33/5085 , G01N2333/43552 , G01N2333/916 , Y10S435/832 , Y10S435/962 , Y10S435/967 , Y10S435/97
摘要： The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B.t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach.
摘要翻译：本发明部分地涉及惊人的和意想不到的发现，与对于Cry毒素敏感的昆虫相比，对苏云金芽孢杆菌Cry毒素具有抗性的昆虫具有可测量的碱性磷酸酶（ALP）活性。这里报道的这个和其他惊人的发现在诸如管理和监测对B.t.的昆虫抗性的发展的领域具有广泛的影响。毒素例如，本发明提供了用于检测ALP活性水平的简单且快速的测定（酶或其它），从而监测昆虫对晶体蛋白质昆虫毒素的抗性的发展。没有事先的动机或建议，使用这种简单而简单的方法来进行阻力监测。

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