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1. 发明授权

US07109309B2 Peptide fructose and protein conjugate with the same 有权
标题翻译：肽果糖和蛋白质缀合物相同
公开(公告)号：US07109309B2
公开(公告)日：2006-09-19
申请号：US10312828
申请日：2001-07-13
申请人： Nobuyuki Shigetoh , Hiroshi Nakayama , Keiko Yugawa , Fumihisa Kitawaki
发明人： Nobuyuki Shigetoh , Hiroshi Nakayama , Keiko Yugawa , Fumihisa Kitawaki
IPC分类号： C07K17/10 , C07K16/18 , C12P21/08 , G01N33/531
CPC分类号： C07K16/16 , C07K9/001 , C07K16/18 , C12P21/005 , Y10S436/823 , Y10S530/809
摘要： It is intended to provide an antibody specific to HbA1c, antibody-producing cells capable of supplying the antibody in a stable state in the future, and a method of constructing the antibody-producing cells without any probability factors, and a method which comprises fusing mouse spleen cells, which have been sensitized with an immunogen composed of a compound containing the following structural formula (I) and a binding protein, with a myeloma-origin cell line, obtaining monoclonal antibody-producing cells by cloning, and then purifying and acquiring the monoclonal antibody produced by these cells into the culture supernatant
摘要翻译：旨在提供对HbA1c特异的抗体，能够在将来以稳定状态供给抗体的抗体产生细胞，以及构建没有任何概率因子的抗体产生细胞的方法，以及包括使小鼠融合的方法已经使用由含有以下结构式（I）的化合物和结合蛋白构成的免疫原与骨髓源起始的细胞系致敏的脾细胞，通过克隆获得产生单克隆抗体的细胞，然后纯化和获得由这些细胞产生的单克隆抗体进入培养上清液

2. 发明授权

US5488114A Labelling color, method of preparing the same and method for detecting methamphetamine with the color 失效
标题翻译：标签颜色，制备方法和颜色检测甲基苯丙胺的方法
公开(公告)号：US5488114A
公开(公告)日：1996-01-30
申请号：US306936
申请日：1994-09-16
申请人： Nobuyuki Shigetoh , Jipsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
发明人： Nobuyuki Shigetoh , Jipsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
IPC分类号： C09B23/08 , G01N33/58 , C07D209/04
CPC分类号： C09B23/083 , G01N33/582 , Y10S435/968 , Y10S436/816 , Y10S436/901 , Y10T436/14
摘要： A labelling color of pentamethine, its intermediate and a method for preparing and using the same to detect methamphetamine with high sensitivity.The invention is exemplified by pentamethine of the following Formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.A method for using the labelling color to detect methamphetamine. The method comprises preparing a solution of the labelling color and methamphetamine-antibody in a buffer, measuring its fluorescent intensity at 660 nm in a 600 nm excited state, adding methamphetamine in a buffer to the solution, measuring its fluorescent intensity similarly, and examining the change in fluorescent intensity between both solutions.
摘要翻译：五甲胺的标记颜色及其中间体及其制备方法，以高灵敏度检测甲基苯丙胺。本发明以下式（1）的五甲基胺为例：其中R1，R2，R3和R4各自为C1-6烷基，X为阴离子的式（1）。一种使用标记颜色检测甲基苯丙胺的方法。该方法包括在缓冲液中制备标记颜色和甲基苯丙胺抗体的溶液，在600nm激发状态下测量其在660nm处的荧光强度，向缓冲液中加入甲基苯丙胺，同样测量其荧光强度，并检查两种溶液之间荧光强度的变化。

3. 发明授权

US07241578B2 Immunoassay method/equipment, biological component measurable toilet, anti-albumin monoclonal antibody, cell strain producing the same, and albumin detection kit 失效
标题翻译：免疫测定法/设备，生物成分可测量马桶，抗白蛋白单克隆抗体，产生相同的细胞株和白蛋白检测试剂盒
公开(公告)号：US07241578B2
公开(公告)日：2007-07-10
申请号：US10441192
申请日：2003-05-20
申请人： Keiko Yugawa , Nobuyuki Shigetoh , Akihito Kamei , Tatsurou Kawamura
发明人： Keiko Yugawa , Nobuyuki Shigetoh , Akihito Kamei , Tatsurou Kawamura
IPC分类号： G01K33/53 , G01K33/536 , G01K33/557 , G01K33/577 , C07K16/18 , C12N5/20 , C12P21/08
CPC分类号： A61B5/415 , A61B5/20 , A61B10/0038 , A61B10/007 , C07K16/18 , G01N33/577 , G01N33/68
摘要： The immunoassay method of this invention measures the content of a subject substance in a sample. The method includes the steps of: (a) preparing a mixed solution by mixing the sample and an antibody solution including a first monoclonal antibody and a second monoclonal antibody capable of specifically binding to the subject substance; and (b) measuring an optical property of the mixed solution. The first monoclonal antibody is capable of binding to a first epitope of the subject substance, and the second monoclonal antibody is capable of binding to a second epitope of the subject substance different from the first epitope. Each of the first and second epitopes exists singly in the subject substance.
摘要翻译：本发明的免疫测定方法测定样品中目标物质的含量。该方法包括以下步骤：（a）通过混合样品和包含第一单克隆抗体和能够特异性结合本发明物质的第二单克隆抗体的抗体溶液来制备混合溶液; 和（b）测量混合溶液的光学性质。第一单克隆抗体能够结合受试物质的第一表位，第二单克隆抗体能够结合不同于第一表位的受试物质的第二表位。第一和第二表位中的每一个单独存在于主题物质中。

4. 发明授权

US5378634A Labelling color for detecting methamphetamine 失效
标题翻译：用于检测甲基苯丙胺的标签颜色
公开(公告)号：US5378634A
公开(公告)日：1995-01-03
申请号：US102426
申请日：1993-08-05
申请人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
发明人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
IPC分类号： C09B23/08 , G01N33/58 , G01N33/00
CPC分类号： C09B23/083 , G01N33/582 , Y10S435/968 , Y10S436/816 , Y10S436/901 , Y10T436/14
摘要： A labelling color which includes a pentamethine of the following formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.
摘要翻译：包括下式（1）的五甲氧基的标记颜色：其中R 1，R 2，R 3和R 4各自为C 1-6烷基，X为阴离子。

5. 发明申请

US20050009102A1 Turbidimetric immunoassay and an apparatus therefor 失效
标题翻译：浊度免疫测定及其设备
公开(公告)号：US20050009102A1
公开(公告)日：2005-01-13
申请号：US10886640
申请日：2004-07-09
申请人： Tatsurou Kawamura , Keiko Yugawa , Akihito Kamei
发明人： Tatsurou Kawamura , Keiko Yugawa , Akihito Kamei
IPC分类号： G01N21/82 , G01N33/52 , G01N33/53 , G01N33/536 , G01N33/557
CPC分类号： G01N33/557
摘要： According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and/or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and/or minimum value is observed, and turbidity level.
摘要翻译：根据本发明，可以在没有稀释步骤等的情况下实现样品溶液中的抗原的可测量的浓度范围的扩大。样品溶液中含有的抗原浓度是根据观察浊度的瞬时现象，观察到最大值和/或最小值时的经过时间以及浊度的最大值和/或最小值来确定的。水平。

6. 发明授权

US06342364B1 Cholesterol sensor and method of determining cholesterol 有权
标题翻译：胆固醇传感器和胆固醇测定方法
公开(公告)号：US06342364B1
公开(公告)日：2002-01-29
申请号：US09831091
申请日：2001-05-03
申请人： Motokazu Watanabe , Keiko Yugawa , Shiro Nankai
发明人： Motokazu Watanabe , Keiko Yugawa , Shiro Nankai
IPC分类号： C12Q160
CPC分类号： C12Q1/60 , C12Q1/005 , G01N33/92 , G01N2800/044 , Y10S435/817
摘要： The present invention provides a sensor that electrochemically determines cholesterol in low density lipoprotein by only one feed of a sample. The sensor has: an electrode system that is mounted on an electrically insulating base plate and includes at least a working electrode and a counter electrode; an enzyme layer formed on the base plate with the electrode system; and a reagent layer that is arranged before the enzyme layer in a sample solution supply path to the electrode system. The enzyme layer includes at least an oxidoreductase and an electron mediator. The reagent layer includes a reagent that depresses reactivity of cholesterol in lipoproteins other than the low density lipoprotein with the oxidoreductase, for example, a reagent that attaches to lipoproteins other than the low density lipoprotein to form a water-soluble complex.
摘要翻译：本发明提供一种传感器，其通过仅一种样品的进料电化学地测定低密度脂蛋白中的胆固醇。传感器具有：电极系统，其安装在电绝缘基板上，并且至少包括工作电极和对电极; 在所述基板上形成有电极系的酶层; 以及被配置在所述酶层之前的试剂溶液供给到所述电极系统的路径中的试剂层。酶层至少包含氧化还原酶和电子介体。试剂层包括抑制低密度脂蛋白以外的脂蛋白中的胆固醇与氧化还原酶的反应性的试剂，例如附着于低密度脂蛋白以外的脂蛋白的试剂，形成水溶性络合物。

7. 发明授权

US07226777B2 Turbidimetric immunoassay and an apparatus therefor 失效
标题翻译：浊度免疫测定及其设备
公开(公告)号：US07226777B2
公开(公告)日：2007-06-05
申请号：US10886640
申请日：2004-07-09
申请人： Tatsurou Kawamura , Keiko Yugawa , Akihito Kamei
发明人： Tatsurou Kawamura , Keiko Yugawa , Akihito Kamei
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N33/557
摘要： According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and/or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and/or minimum value is observed, and turbidity level.
摘要翻译：根据本发明，可以在没有稀释步骤等的情况下实现样品溶液中的抗原的可测量的浓度范围的扩大。样品溶液中含有的抗原浓度是根据观察浊度的瞬时现象，观察到最大值和/或最小值时的经过时间以及浊度的最大值和/或最小值来确定的。水平。

8. 发明授权

US07202041B2 Immunoreaction measurement method 有权
标题翻译：免疫反应测定法
公开(公告)号：US07202041B2
公开(公告)日：2007-04-10
申请号：US10516067
申请日：2003-12-09
申请人： Akihito Kamei , Tatsurou Kawamura , Keiko Yugawa
发明人： Akihito Kamei , Tatsurou Kawamura , Keiko Yugawa
IPC分类号： G01N33/53 , G01N33/536 , G01N21/00 , C07K16/00
CPC分类号： G01N33/5306 , Y10S435/961 , Y10S436/805
摘要： The present invention relates to an immunoreaction measuring method for measuring an antigen or antibody contained as a subject substance in a sample, wherein the sample was mixed with at least one compound selected from the group consisting of dicarboxylic acids having a hydroxyl group, dicarboxylic acids having a double bond, straight-chain dicarboxylic acids expressed by the chemical formula (1): HOOC(CH2)nCOOH (n is an integer), and the salts of these dicarboxylic acids, and an antibody or antigen as a specifically binding substance capable of specifically binding to the subject substance, to obtain an acidic reaction solution, and then an antigen-antibody complex, generated by an antigen-antibody reaction of the subject substance with the specifically binding substance in the reaction solution was detected. This makes it possible to improve a measurement value and to relax a limitation of a measurement range due to a zone phenomenon that occurs in an antigen-excess region.
摘要翻译：本发明涉及一种用于测量样品中作为主体物质的抗原或抗体的免疫反应测定方法，其中将样品与选自由具有羟基的二羧酸组成的组中选择的至少一种化合物混合，双键，由化学式（1）表示的直链二羧酸：HOOC（CH 2 CH 2）n COOH（n为整数），其盐这些二羧酸和作为特异性结合物质的能够与本发明物质特异性结合的抗体或抗原，获得酸性反应溶液，然后将抗原 - 抗体复合物通过本发明物质的抗原 - 抗体反应与检测反应溶液中特异性结合物质。这使得可以改善测量值并且放松由于在抗原过量区域中发生的区域现象引起的测量范围的限制。

9. 发明授权

US07005048B1 Glucose sensor 有权
标题翻译：葡萄糖传感器
公开(公告)号：US07005048B1
公开(公告)日：2006-02-28
申请号：US09807692
申请日：2000-10-02
申请人： Motokazu Watanabe , Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai , Junko Nakayama , Shoji Miyazaki , Hideyuki Baba
发明人： Motokazu Watanabe , Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai , Junko Nakayama , Shoji Miyazaki , Hideyuki Baba
IPC分类号： G01N27/327
CPC分类号： C12Q1/006
摘要： The present invention provides a high-performance glucose sensor having excellent storage stability and an improved response characteristic. This sensor comprises: an electrically insulating base plate; an electrode system including at least a working electrode and a counter electrode formed on the base plate; and a reaction layer containing at least pyrrolo-quinoline quinone dependent glucose dehydrogenase, formed in contact with or in the vicinity of the electrode system, and the reaction layer contains at least one kind of additive selected from the group consisting of gluconic acid and salts thereof.
摘要翻译：本发明提供一种具有优异的储存稳定性和改善的响应特性的高性能葡萄糖传感器。该传感器包括：电绝缘基板; 电极系统，其至少包括形成在所述基板上的工作电极和对电极; 和至少含有与电极体系接触或附近形成的吡咯并喹啉醌依赖型葡萄糖脱氢酶的反应层，反应层含有选自葡萄糖酸及其盐的至少一种添加剂。

10. 发明授权

US06174723B1 Cocaine derivative, protein conjugate thereof, monoclonal antibody producing cell line, method for preparing the cell line and monoclonal antibody 失效
标题翻译：可卡因衍生物，其蛋白质缀合物，产生单克隆抗体的细胞系，制备细胞系的方法和单克隆抗体
公开(公告)号：US06174723B1
公开(公告)日：2001-01-16
申请号：US08982963
申请日：1997-12-02
申请人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C12N506
CPC分类号： G01N33/946 , A61K47/60 , A61K47/643 , C07D451/12 , C07K16/16
摘要： A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives. A monoclonal antibody, a monoclonal antibody producing cell line, and a method for producing the monoclonal antibody producing cell line by using the above cocaine-protein conjugate as an immunogen is also described. The method of the invention comprises preparing a cocaine-protein conjugate with a pyridyl dithiococaine derivative of Formula (I): where R is either H or R′; and where R′ is: wherein n=1 to 4 and Ph is a benzene ring, and wherein said monoclonal antibody detects 10−9 M cocaine; and which derivative binds to a protein by a disulfide bond; immunizing A/J mouse with the conjugate; fusing its spleen cell and a myeloma cell line; cloning the fusion to produce a monoclonal antibody producing cell line; culturing the cell line, and purifying a supernatant of the culture to form a monoclonal antibody to specifically bind to cocaine or cocaine derivatives.
摘要翻译：通过使用具有甲氧基羰基和苯甲酰基的可卡因衍生物容易地制备可卡因 - 蛋白质结合物的方法。该缀合物可用于检测可卡因或可卡因衍生物。还描述了单克隆抗体，产生单克隆抗体的细胞系，以及通过使用上述可卡因蛋白质缀合物作为免疫原来制备产生单克隆抗体的细胞系的方法。本发明的方法包括用式（I）的吡啶基二硫代可卡因衍生物制备可卡因蛋白质结合物：其中R是H或R'; 其中R'为：其中n = 1-4，Ph为苯环，其中所述单克隆抗体检测10-9M可卡因; 哪个衍生物通过二硫键与蛋白质结合; 用缀合物免疫A / J小鼠; 融合其脾细胞和骨髓瘤细胞系; 克隆融合物以产生产生单克隆抗体的细胞系; 培养细胞系，并纯化培养物的上清液以形成特异性结合可卡因或可卡因衍生物的单克隆抗体。

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