专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06342364B1 Cholesterol sensor and method of determining cholesterol 有权
标题翻译：胆固醇传感器和胆固醇测定方法
公开(公告)号：US06342364B1
公开(公告)日：2002-01-29
申请号：US09831091
申请日：2001-05-03
申请人： Motokazu Watanabe , Keiko Yugawa , Shiro Nankai
发明人： Motokazu Watanabe , Keiko Yugawa , Shiro Nankai
IPC分类号： C12Q160
CPC分类号： C12Q1/60 , C12Q1/005 , G01N33/92 , G01N2800/044 , Y10S435/817
摘要： The present invention provides a sensor that electrochemically determines cholesterol in low density lipoprotein by only one feed of a sample. The sensor has: an electrode system that is mounted on an electrically insulating base plate and includes at least a working electrode and a counter electrode; an enzyme layer formed on the base plate with the electrode system; and a reagent layer that is arranged before the enzyme layer in a sample solution supply path to the electrode system. The enzyme layer includes at least an oxidoreductase and an electron mediator. The reagent layer includes a reagent that depresses reactivity of cholesterol in lipoproteins other than the low density lipoprotein with the oxidoreductase, for example, a reagent that attaches to lipoproteins other than the low density lipoprotein to form a water-soluble complex.
摘要翻译：本发明提供一种传感器，其通过仅一种样品的进料电化学地测定低密度脂蛋白中的胆固醇。传感器具有：电极系统，其安装在电绝缘基板上，并且至少包括工作电极和对电极; 在所述基板上形成有电极系的酶层; 以及被配置在所述酶层之前的试剂溶液供给到所述电极系统的路径中的试剂层。酶层至少包含氧化还原酶和电子介体。试剂层包括抑制低密度脂蛋白以外的脂蛋白中的胆固醇与氧化还原酶的反应性的试剂，例如附着于低密度脂蛋白以外的脂蛋白的试剂，形成水溶性络合物。

2. 发明授权

US07005048B1 Glucose sensor 有权
标题翻译：葡萄糖传感器
公开(公告)号：US07005048B1
公开(公告)日：2006-02-28
申请号：US09807692
申请日：2000-10-02
申请人： Motokazu Watanabe , Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai , Junko Nakayama , Shoji Miyazaki , Hideyuki Baba
发明人： Motokazu Watanabe , Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai , Junko Nakayama , Shoji Miyazaki , Hideyuki Baba
IPC分类号： G01N27/327
CPC分类号： C12Q1/006
摘要： The present invention provides a high-performance glucose sensor having excellent storage stability and an improved response characteristic. This sensor comprises: an electrically insulating base plate; an electrode system including at least a working electrode and a counter electrode formed on the base plate; and a reaction layer containing at least pyrrolo-quinoline quinone dependent glucose dehydrogenase, formed in contact with or in the vicinity of the electrode system, and the reaction layer contains at least one kind of additive selected from the group consisting of gluconic acid and salts thereof.
摘要翻译：本发明提供一种具有优异的储存稳定性和改善的响应特性的高性能葡萄糖传感器。该传感器包括：电绝缘基板; 电极系统，其至少包括形成在所述基板上的工作电极和对电极; 和至少含有与电极体系接触或附近形成的吡咯并喹啉醌依赖型葡萄糖脱氢酶的反应层，反应层含有选自葡萄糖酸及其盐的至少一种添加剂。

3. 发明授权

US06656702B1 Biosensor containing glucose dehydrogenase 有权
标题翻译：含有葡萄糖脱氢酶的生物传感器
公开(公告)号：US06656702B1
公开(公告)日：2003-12-02
申请号：US09345676
申请日：1999-06-30
申请人： Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai
发明人： Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： C12Q132
CPC分类号： C12Q1/005
摘要： A biosensor that enables rapid and simple quantitation of a substrate with high accuracy and demonstrates an excellent preservation characteristic by best retaining enzyme activity is disclosed. The biosensor comprises an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on the base plate, and a reaction layer containing at least an enzyme and a sugar. The reaction layer may be formed on the electrode system. The enzyme may be selected from glucose oxidase, glucose dehydrogenase, and fructose dehydrogenase. The sugar is trehalose, sucrose, glycerol, mannitol, and ribose. If the enzyme selected is glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the use of trehalose is preferable.
摘要翻译：公开了一种生物传感器，其能够高精度地快速和简单地定量基质，并通过最佳的保留酶活性表现出优异的保存特性。生物传感器包括电绝缘基板，至少包括形成在基板上的工作电极和对电极的电极系统和至少含有酶和糖的反应层。反应层可以形成在电极系统上。酶可以选自葡萄糖氧化酶，葡萄糖脱氢酶和果糖脱氢酶。糖是海藻糖，蔗糖，甘油，甘露糖醇和核糖。如果选择的酶是辅酶是吡咯并喹啉醌的葡萄糖脱氢酶，则优选使用海藻糖。

4. 发明授权

US06773564B1 Glucose sensor 有权
标题翻译：葡萄糖传感器
公开(公告)号：US06773564B1
公开(公告)日：2004-08-10
申请号：US09406832
申请日：1999-09-28
申请人： Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai , Junko Iwata , Shoji Miyazaki , Hideyuki Baba , Seiji Takeshima
发明人： Keiko Yugawa , Toshihiko Yoshioka , Shiro Nankai , Junko Iwata , Shoji Miyazaki , Hideyuki Baba , Seiji Takeshima
IPC分类号： G01N27327
CPC分类号： C12Q1/006
摘要： The present invention provides a high performance glucose sensor which can demonstrate high stability against preservation and produces only a low blank value. The glucose sensor comprises an electrically insulating base plate, an electrode system formed on the base plate, and a reaction layer which is formed in contact with or in the vicinity of the electrode system and contains at least a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the reaction layer further containing an additive such as phthalic acid.
摘要翻译：本发明提供了一种高性能葡萄糖传感器，其可以显示对保存的高稳定性并且仅产生低的空白值。葡萄糖传感器包括电绝缘基板，形成在基板上的电极系统和与电极系统接触或附近形成的反应层，并且至少含有辅酶是吡咯并喹啉的葡萄糖脱氢酶醌，反应层还含有邻苯二甲酸等添加剂。

5. 发明授权

US07267750B2 Biosensor 有权
标题翻译：生物传感器
公开(公告)号：US07267750B2
公开(公告)日：2007-09-11
申请号：US10466561
申请日：2002-01-10
申请人： Motokazu Watanabe , Tomohiro Yamamoto , Miwa Hasegawa , Shin Ikeda , Shiro Nankai
发明人： Motokazu Watanabe , Tomohiro Yamamoto , Miwa Hasegawa , Shin Ikeda , Shiro Nankai
IPC分类号： G01N27/327
CPC分类号： C12Q1/005 , C12Q1/004
摘要： A biosensor comprising: an electrically insulating base plate; an electrode system comprising a working electrode and a counter electrode formed on the base plate; a cover member which is joined to the base plate to form a sample solution supply pathway for supplying a sample solution to the electrode system between the cover member and the base plate; and a reagent system comprising at least an oxidoreductase and an electron mediator disposed in the sample solution supply pathway, wherein the electron mediator is provided on the base plate and the oxidoreductase is provided on the cover member such that the electron mediator and the oxidoreductase are not in contact with each other.
摘要翻译：一种生物传感器，包括：电绝缘基板; 电极系统，包括形成在所述基板上的工作电极和对电极; 盖构件，其连接到所述基板，以形成用于将样品溶液供应到所述盖构件和所述基板之间的所述电极系统的样品溶液供给路径; 以及试剂系统，其至少包含设置在样品溶液供给路径中的氧化还原酶和电子介体，其中，所述电子介体设置在所述底板上，所述氧化还原酶设置在所述覆盖部件上，使得所述电子介体和所述氧化还原酶不是彼此接触。

6. 发明授权

US07235170B2 Biosensor 有权
标题翻译：生物传感器
公开(公告)号：US07235170B2
公开(公告)日：2007-06-26
申请号：US10344671
申请日：2002-05-13
申请人： Motokazu Watanabe , Takahiro Nakaminami , Shin Ikeda , Shiro Nankai
发明人： Motokazu Watanabe , Takahiro Nakaminami , Shin Ikeda , Shiro Nankai
IPC分类号： C12Q1/26
CPC分类号： C12Q1/006 , C12Q1/001 , C12Q1/54
摘要： A biosensor that is highly responsive and capable of rapid and highly sensitive quantification of a specific component contained in a sample is provided. The biosensor of this invention comprises: an electrically insulating base plate; an electrode system comprising a working electrode and a counter electrode disposed on the base plate; and a reagent system comprising an oxidoreductase which catalyzes the oxidation reaction of glucose, gluconolactonase and a buffer. The buffer is selected from the group consisting of phthalic acid and its salts, maleic acid and its salts, succinic acid and its salts, phospholic acid and its salts, acetic acid and its salts, boric acid and its salts, citric acid and its salts, glycine, tris(hydroxymethyl)aminomethane, piperazine-N,N′-bis(2-ethane sulfonic acid) and the like.
摘要翻译：提供了一种生物传感器，其具有高响应性并且能够对包含在样品中的特定成分进行快速和高灵敏度的定量。本发明的生物传感器包括：电绝缘基板; 电极系统，包括设置在所述基板上的工作电极和对置电极; 以及包含催化葡萄糖，葡糖酸内切酶和缓冲液的氧化反应的氧化还原酶的试剂体系。缓冲剂选自邻苯二甲酸及其盐，马来酸及其盐，琥珀酸及其盐，磷酸及其盐，乙酸及其盐，硼酸及其盐，柠檬酸及其盐，甘氨酸，三（羟甲基）氨基甲烷，哌嗪-N，N'-双（2-乙磺酸）等。

7. 发明授权

US06436255B2 Biosensor 失效
标题翻译：生物传感器
公开(公告)号：US06436255B2
公开(公告)日：2002-08-20
申请号：US09764142
申请日：2001-01-19
申请人： Tomohiro Yamamoto , Motokazu Watanabe , Shin Ikeda , Shiro Nankai
发明人： Tomohiro Yamamoto , Motokazu Watanabe , Shin Ikeda , Shiro Nankai
IPC分类号： G01N27327
CPC分类号： C12Q1/005
摘要： The present invention provides a biosensor which enables prompt quantification by facilitating introduction of a sample solution even when the amount of the sample solution is small and improving dissolution of reagents. The biosensor comprises an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on the base plate; a cover member integrated into the base plate to form a sample solution supply pathway for supplying a sample solution to the electrode system between the cover member and the base plate; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of the reaction reagent system, wherein the carrier is partially or wholly disposed outside the sample solution supply pathway.
摘要翻译：本发明提供一种生物传感器，其即使在样品溶液量少且改善试剂溶解的情况下也能够通过促进样品溶液的引入而迅速进行定量。生物传感器包括绝缘基板; 电极系统，其至少包括形成在所述基板上的测量电极和对电极; 集成到所述基板中以形成用于将样品溶液供应到所述盖构件和所述基板之间的所述电极系统的样品溶液供给路径的盖构件; 至少包括电子介体和氧化还原酶的反应试剂系统; 和至少支持反应试剂体系的电子介体或氧化还原酶的载体，其中载体被部分或全部置于样品溶液供给路径的外部。

8. 发明授权

US6104940A Electrode probe and body fluid examination equipment including the same 有权
标题翻译：电极探针和体液检查设备包括相同
公开(公告)号：US6104940A
公开(公告)日：2000-08-15
申请号：US326350
申请日：1999-06-04
申请人： Motokazu Watanabe , Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai
发明人： Motokazu Watanabe , Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： G01N27/30 , A61B5/00 , A61B5/145 , A61B5/1486 , A61B5/15 , A61B5/157 , A61B5/05
CPC分类号： A61B5/1486 , A61B5/6848
摘要： Disclosed is an electrode probe and a body fluid examination equipment enabling examination of a trace amount of body fluid. The electrode probe comprises an insulating needle member, a working electrode lead disposed in the needle member, a working electrode whose lead is protruding outside from the needle member, a counter electrode disposed on the surface of the needle member, a lead connected to the counter electrode, and a reagent layer disposed in contact with or in the vicinity of the working and counter electrodes of the electrode system. When a trace amount of body fluid leaking on the skin surface is brought into contact with the reagent layer, the reagent layer dissolves which results in electron transfer whereby information about the concentration of analyte in the body fluid can be obtained from the electrode system.
摘要翻译：公开了能够检测微量体液的电极探针和体液检查装置。电极探针包括绝缘针构件，设置在针构件中的工作电极引线，其引线从针构件向外突出的工作电极，设置在针构件表面上的对电极，连接到计数器的引线电极和设置成与电极系统的工作电极和对电极接触或附近的试剂层。当皮肤表面泄漏的微量体液与试剂层接触时，试剂层溶解，导致电子转移，从而可以从电极系统获得关于体液中分析物浓度的信息。

9. 发明授权

US06776888B2 Biosensor 失效
标题翻译：生物传感器
公开(公告)号：US06776888B2
公开(公告)日：2004-08-17
申请号：US10089289
申请日：2002-03-26
申请人： Tomohiro Yamamoto , Miwa Hasegawa , Motokazu Watanabe , Shin Ikeda , Shiro Nankai
发明人： Tomohiro Yamamoto , Miwa Hasegawa , Motokazu Watanabe , Shin Ikeda , Shiro Nankai
IPC分类号： G01N27327
CPC分类号： C12Q1/004 , G01N27/3272
摘要： The present invention provides a biosensor that enables highly-accurate measurement of a sample solution including a solid component like hemocytes and has a little variation in response. The biosensor includes: an insulating base plate, an electrode system having at least a working electrode and a counter electrode provided on the base plate, a cover member that is combined with the base plate to define a sample solution supply pathway for leading a sample solution from a sample supply unit to the electrode system, a reaction reagent system including at least an oxidation-reduction enzyme and an electron mediator, and a filter disposed between the electrode system and the sample supply unit in the sample solution supply pathway. The biosensor has a space that encircles surface of the filter in an area from one end of the filter close to the sample supply unit to the other end of the filter close to the electrode system. This arrangement effectively prevents the solid component like hemocytes from flowing into the electrode system without being filtered out by the filter.
摘要翻译：本发明提供一种生物传感器，其能够高精度地测量包含像血细胞这样的固体成分的样品溶液，并且响应性变化很小。生物传感器包括：绝缘基板，至少具有工作电极和设置在基板上的对电极的电极系统，与基板结合以限定用于引导样品溶液的样品溶液供给路径的盖构件从样品供给单元到电极系统，至少包含氧化还原酶和电子介体的反应试剂系统，以及设置在样品溶液供给路径中的电极系统和样品供给单元之间的过滤器。生物传感器具有在过滤器的一端靠近样品供给单元的一个区域中的过滤器的表面周围到靠近电极系统的过滤器的另一端的空间。这种布置有效地防止诸如血细胞的固体组分流入电极系统而不被过滤器过滤掉。

10. 发明授权

US06383819B1 Method for determining cholesterol and sensor applicable to the same 有权
标题翻译：胆固醇和传感器的测定方法
公开(公告)号：US06383819B1
公开(公告)日：2002-05-07
申请号：US09492979
申请日：2000-01-27
申请人： Motokazu Watanabe , Toshihiko Yoshioka , Shiro Nankai
发明人： Motokazu Watanabe , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： G01N33543
CPC分类号： G01N27/3272 , G01N33/92 , G01N2800/044 , G01N2800/52 , Y10S435/803 , Y10S435/814 , Y10S435/817 , Y10S435/962 , Y10S435/973 , Y10S436/806 , Y10S436/815 , Y10S436/824 , Y10S436/825 , Y10T436/25375 , Y10T436/255
摘要： The present disclosure relates to a method for determining cholesterol in low density lipoprotein comprising the steps of (a) measuring total cholesterol level in a sample containing at least high density lipoprotein, low density lipoprotein, very low density lipoprotein and chylomicron, and (b) measuring cholesterol levels in the high density lipoprotein, very low density lipoprotein and chylomicron in the sample, wherein the cholesterol level in the low density lipoprotein is determined by subtracting a value obtained in step (b) from a value obtained in step (a). The present invention enables concurrent determination of cholesterol level in low density lipoprotein and total cholesterol level, facilitating acquisition of two types of biological information at a time.
摘要翻译：本公开涉及一种用于测定低密度脂蛋白胆固醇的方法，包括以下步骤：（a）测量含有至少高密度脂蛋白，低密度脂蛋白，极低密度脂蛋白和乳糜微粒的样品中的总胆固醇水平，和（b）测量样品中高密度脂蛋白，极低密度脂蛋白和乳糜微粒中的胆固醇水平，其中通过从步骤（a）中获得的值减去步骤（b）中获得的值来确定低密度脂蛋白中的胆固醇水平。本发明能够同时测定低密度脂蛋白和总胆固醇水平的胆固醇水平，便于一次获取两种生物信息。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式