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    • 9. 发明授权
    • Apparatus for collecting lysozyme from egg white by adsorption
    • 用于通过吸附从蛋白中收集溶菌酶的装置
    • US4705755A
    • 1987-11-10
    • US756552
    • 1985-07-19
    • Mineo HasegawaKitao Ozaki
    • Mineo HasegawaKitao Ozaki
    • B01J47/00C12M1/00C12N9/00C12N9/36C12M1/02B01D15/02
    • C12N9/2462B01J47/001C12N9/00Y10S435/815
    • A batch system apparatus for collecting lysozyme from egg white by adsorption on an ion-exchange resin, which includes a lysozyme adsorption tank, equipped therein with a stirring mechanism for stirring an egg white solution and an ion-exchange resin, having a strainer provided in a lower portion thereof, having liquid discharge pipes connected thereto at a position below the strainer, preferably at the bottom of the tank, and at the side wall above the strainer at a height such that the resin when left at rest will not overflow, respectively, and further having a resin discharge pipe connected thereto at the lower portion of the side wall, above and in the vicinity of the strainer; a positive displacement pump connected to the liquid discharge pipes for transferring the egg white solution after removal of lysozyme to a reservoir tank; and a centrifugal pump connected to the resin discharge pipe for transferring the mixture of the resin and water to a separate tank, whereby collection by adsorption can be made simple, convenient for use and also suitable for a small-quantity treatment.
    • 一种分批系统装置,用于通过在离子交换树脂上吸附从蛋白中收集溶菌酶,所述离子交换树脂包括溶菌酶吸附罐,其中装有搅拌机构用于搅拌蛋清溶液和离子交换树脂,所述搅拌机构具有设置在 其下部具有在过滤器下方的位置,优选地在罐的底部连接到其上的液体排出管,以及在过滤器上方的侧壁处,其高度使得树脂在静止时不会溢出 并且在侧壁的下部,过滤器的上方和附近还具有与其连接的树脂排出管; 连接到液体排出管的正排量泵,用于在将溶菌酶移除到储存罐之后转移蛋白溶液; 以及连接到树脂排出管的离心泵,用于将树脂和水的混合物转移到单独的罐中,由此通过吸附进行收集可以简单,使用方便,并且也适合于少量处理。