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1. 发明申请

US20080084008A1 Devices and methods for the synthesis of nucleic acids 失效
标题翻译：用于合成核酸的装置和方法
公开(公告)号：US20080084008A1
公开(公告)日：2008-04-10
申请号：US10776694
申请日：2004-02-12
申请人： Nam Ngo , Laurent Jaquinod , Hong Wang
发明人： Nam Ngo , Laurent Jaquinod , Hong Wang
IPC分类号： B29C39/36 , B01J8/00
CPC分类号： B01L3/50255 , B01L2200/12 , B01L2300/0829
摘要： Cylindrical devices (frits) are prepared by embedding aminoalkyl- or mercaptoalkyl-modified Controlled Pore Glass (CPG) in high-density polyethylene. Methods and devices pertaining to their use in the synthesis of nucleic acids are described. A reusable synthesis column or a reusable 96-chamber synthesis plate have been designed to hold one to 96 of the said frits that are inserted reproducibly into the synthesis chambers with a frit insertor. A short gas surpressure is required to drive entry of chemical reagents into the said frit. Reagents are retained into the frit until a second, longer surpressure is applied to drain the said reagents.
摘要翻译：通过将氨基烷基或巯基烷基改性的控制孔玻璃（CPG）嵌入高密度聚乙烯中来制备圆柱形装置（玻璃料）。描述了它们在核酸合成中的用途的方法和装置。已经设计了可重复使用的合成柱或可重复使用的96室合成板，以容纳一至96个所述玻璃料，其被再次插入具有玻璃料插入物的合成室中。需要短时间的气体压力来驱动化学试剂进入所述玻璃料。将试剂保留在玻璃料中，直到施加第二次较长的耐压以排出所述试剂。

2. 发明申请

US20050182241A1 Universal support for nucleic acid synthesis 审中-公开
标题翻译：普遍支持核酸合成
公开(公告)号：US20050182241A1
公开(公告)日：2005-08-18
申请号：US10776695
申请日：2004-02-12
申请人： Nam Ngo , Laurent Jaquinod , Hong Wang
发明人： Nam Ngo , Laurent Jaquinod , Hong Wang
IPC分类号： C07F7/18 , C07F7/21 , C07H21/04 , C08G63/48
CPC分类号： C07H21/04 , C07B2200/11 , C07F7/1804
摘要： Polysubstituted catechol-based universal solid supports suitable for synthesizing oligonucleotides have been prepared. Following synthesis, cleavage of the oligonucleotide from the solid support and catechol-assisted elimination of the 3′-phosphate group is accomplished by treatment with standard basic media such as ammonium hydroxyde or1 methylamine.
摘要翻译：已经制备了适用于合成寡核苷酸的基于多取代儿茶酚的通用固体支持物。合成后，通过用标准碱性介质如氢氧化铵或叔丁醇处理，可以通过用固体载体和儿茶酚辅助消除3'-磷酸基团来切割寡核苷酸。

3. 发明授权

US07691316B2 Devices and methods for the synthesis of nucleic acids 失效
标题翻译：用于合成核酸的装置和方法
公开(公告)号：US07691316B2
公开(公告)日：2010-04-06
申请号：US10776694
申请日：2004-02-12
申请人： Nam Ngo , Laurent Jaquinod , Hong Wang
发明人： Nam Ngo , Laurent Jaquinod , Hong Wang
IPC分类号： B29C39/36 , B29C67/04
CPC分类号： B01L3/50255 , B01L2200/12 , B01L2300/0829
摘要： Cylindrical devices (frits) are prepared by embedding aminoalkyl- or mercaptoalkyl-modified Controlled Pore Glass (CPG) in high-density polyethylene. Methods and devices pertaining to their use in the synthesis of nucleic acids are described. A reusable synthesis column or a reusable 96-chamber synthesis plate have been designed to hold one to 96 of the said frits that are inserted reproducibly into the synthesis chambers with a frit insertor. A short gas surpressure is required to drive entry of chemical reagents into the said frit. Reagents are retained into the frit until a second, longer surpressure is applied to drain the said reagents.
摘要翻译：通过将氨基烷基或巯基烷基改性的控制孔玻璃（CPG）嵌入高密度聚乙烯中来制备圆柱形装置（玻璃料）。描述了它们在核酸合成中的用途的方法和装置。已经设计了可重复使用的合成柱或可重复使用的96室合成板，以容纳一至96个所述玻璃料，其被再次插入具有玻璃料插入物的合成室中。需要短时间的气体压力来驱动化学试剂进入所述玻璃料。将试剂保留在玻璃料中，直到施加第二次较长的耐压以排出所述试剂。

4. 发明申请

US20100183488A1 DEVICES AND METHODS FOR THE SYNTHESIS OF NUCLEIC ACIDS 审中-公开
标题翻译：用于合成核酸的装置和方法
公开(公告)号：US20100183488A1
公开(公告)日：2010-07-22
申请号：US12749754
申请日：2010-03-30
申请人： Nam Ngo , Laurent Jaquinod , Hong Wang
发明人： Nam Ngo , Laurent Jaquinod , Hong Wang
IPC分类号： B01J19/00 , C08J5/00
CPC分类号： B01L3/50255 , B01L2200/12 , B01L2300/0829
摘要： Cylindrical devices (frits) are prepared by embedding aminoalkyl- or mercaptoalkyl-modified Controlled Pore Glass (CPG) in high-density polyethylene. Methods and devices pertaining to their use in the synthesis of nucleic acids are described. A reusable synthesis column or a reusable 96-chamber synthesis plate have been designed to hold one to 96 of the said frits that are inserted reproducibly into the synthesis chambers with a frit insertor. A short gas surpressure is required to drive entry of chemical reagents into the said frit. Reagents are retained into the frit until a second, longer surpressure is applied to drain the said reagents.
摘要翻译：通过将氨基烷基或巯基烷基改性的控制孔玻璃（CPG）嵌入高密度聚乙烯中来制备圆柱形装置（玻璃料）。描述了它们在核酸合成中的用途的方法和装置。已经设计了可重复使用的合成柱或可重复使用的96室合成板，以容纳一至96个所述玻璃料，其被再次插入具有玻璃料插入物的合成室中。需要短时间的气体压力来驱动化学试剂进入所述玻璃料。将试剂保留在玻璃料中，直到施加第二次较长的耐压以排出所述试剂。

5. 发明申请

US20080033158A1 Multi layer chromatography of nucleic acids 有权
标题翻译：核酸多层色谱法
公开(公告)号：US20080033158A1
公开(公告)日：2008-02-07
申请号：US11888490
申请日：2007-07-31
申请人： Nam Ngo , Laurent Jaquinod
发明人： Nam Ngo , Laurent Jaquinod
IPC分类号： C07H21/04
CPC分类号： C12N15/101
摘要： Herein, we introduce three multimodal SPE methods using two to (n) purification columns to separate full length 5′-DMT-on oligonucleotides with size ranging from 40 to 180-mers from short length 5′-DMT-on oligonucleotides. Two of the said methods require using some columns sequentially with the collection and reprocessing of an intermediate fraction and are used for oligonucleotides with length ranging from 70 to 180-mers. A third method is carried out with columns stacked and used in series and is best used to purify oligonucleotides with length ranging from 40 to 80-mers. Preferentially, a series of stacked columns contains from top to bottom hydrophobic porous sorbents with increasing pore sizes. Short length DMT-on oligonucleotides arise from depurination or branching during phosphoramidite based synthesis. Reversed phase partitioning and binding of short length DMT-on oligonucleotides take place simultaneously with the size exclusion of the full length DMT-on oligonucleotides. In the presence of a high ionic strength buffer, the short length DMT-on oligonucleotides bind to the top stacked columns while the less hydrophobic contaminant or DMT-off failures do not bind and/or are being washed off. In a stacked configuration, the full length DMT-on oligonucleotides are retained by the bottom column while in a sequential configuration, full length DMT-on oligonucleotides are collected and reprocessed. After detritylation of the full length oligonucleotides from the bottom column or last column in a sequence, full length nucleic acids are eluted with purity typically ranging from 90 to 95% for oligonucleotides about 80-mers in size and purity around 80 to 90% for oligonucleotides about 150-mers in size. This invention yields purified long oligonucleotides at a fraction of traditional purification costs which could spur their wider use in biological applications.
摘要翻译：在这里，我们引入三种多模态SPE方法，使用两个到（n）纯化柱来分离大小范围在从40-180毫米的短长度5'-DMT-寡核苷酸的全长5'-DMT-寡核苷酸。所述方法中的两个需要依次使用一些列进行中间馏分的收集和重新处理，并且用于长度范围为70-180的寡核苷酸。第三种方法用柱堆叠并串联使用，最适用于纯化长度范围为40至80毫升的寡核苷酸。优选地，一系列堆叠的柱含有从上到下的具有增加的孔径的疏水性多孔吸附剂。短的DMT-寡核苷酸产生于基于亚磷酰胺的合成期间的去除或分支。反转相分配和短长度DMT-寡核苷酸的结合与全长DMT-寡核苷酸的大小排除同时进行。在存在高离子强度缓冲液的情况下，短长度DMT-on寡核苷酸结合顶部堆叠柱，而疏水性较小的污染物或DMT脱离失败不结合和/或被洗掉。在堆叠配置中，全长DMT-on寡核苷酸由底部柱保留，而在顺序配置中，全长DMT-寡核苷酸被收集并再处理。在序列中的底部柱或最后一列的全长寡核苷酸脱结合后，对于寡核苷酸，对于寡核苷酸，对于寡核苷酸，大约80个多聚体的寡核苷酸的纯度通常为90-95％，对于寡核苷酸，纯度约为80-90％，全长核酸大约150米。本发明以传统净化成本的一小部分产生纯化的长寡核苷酸，这可以促进其在生物应用中的更广泛的应用。

6. 发明申请

US20070135626A1 Activator bound solid supports for nucleic acid synthesis via the phosphoramidite approach 有权
标题翻译：活化剂通过亚磷酰胺方法结合核酸合成的固体支持物
公开(公告)号：US20070135626A1
公开(公告)日：2007-06-14
申请号：US11301020
申请日：2005-12-12
申请人： Nam Ngo , Laurent Jaquinod
发明人： Nam Ngo , Laurent Jaquinod
IPC分类号： C07H21/00 , C08G63/91
CPC分类号： C07H21/00
摘要： The present invention relates to improved methods for the preparation of nucleic acids. More particularly, conventional solid supports used for nucleic acid synthesis are derivatized with activators having pKas within the 4 to 7 range. Preferentially, CPG-based solid supports are reacted with trialkoxysilanes containing an activator moiety such as pyridine. During each deblocking step of the nucleic acid synthesis cycle, bound pyridiniums are generated, yielding a weak acidic medium spreads throughout the solid support. The bound activators efficiently activate the phosphoramidite reagents towards coupling with 5′-hydroxynucleosides bound to the solid supports, thus eliminating or supplementing external deliveries of activator during the coupling steps.
摘要翻译：本发明涉及用于制备核酸的改进方法。更具体地，用于核酸合成的常规固体支持物用具有4至7范围内的pKas的激活剂衍生化。优选地，将基于CPG的固体载体与含有活化剂部分如吡啶的三烷氧基硅烷反应。在核酸合成循环的每个去封闭步骤期间，产生结合的吡啶鎓，产生弱酸性介质遍及整个固体支持物。结合的活化剂有效地活化亚磷酰胺试剂以与与固体支持物结合的5'-羟基核苷偶联，从而在偶联步骤期间消除或补充活化剂的外部输送。

7. 发明申请

US20060160099A1 Substrate preparation process 审中-公开
公开(公告)号：US20060160099A1
公开(公告)日：2006-07-20
申请号：US11015257
申请日：2004-12-16
申请人： Martin Goldberg , Martin Diggelman , Earl Hubbell , Glenn McGall , Nam Ngo , MacDonald Morris , Mel Yamamoto , Jennifer Tan , Richard Rava
发明人： Martin Goldberg , Martin Diggelman , Earl Hubbell , Glenn McGall , Nam Ngo , MacDonald Morris , Mel Yamamoto , Jennifer Tan , Richard Rava
IPC分类号： C12Q1/68 , C12M1/34 , B05D3/02
CPC分类号： C07K1/047 , B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y30/00 , C07B2200/11 , C07H21/00 , C40B40/06 , C40B40/10 , C40B60/14
摘要： The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture of polymer arrays.

8. 发明申请

US20050181396A1 Substrate preparation process 审中-公开
标题翻译：基材制备工艺
公开(公告)号：US20050181396A1
公开(公告)日：2005-08-18
申请号：US11016629
申请日：2004-12-17
申请人： Martin Goldberg , Martin Diggelman , Earl Hubbell , Glenn McGall , Nam Ngo , MacDonald Morris , Mel Yamamoto , Jennifer Tan , Richard Rava
发明人： Martin Goldberg , Martin Diggelman , Earl Hubbell , Glenn McGall , Nam Ngo , MacDonald Morris , Mel Yamamoto , Jennifer Tan , Richard Rava
IPC分类号： B01J19/00 , C07B61/00 , C07H21/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B60/14 , C12Q1/68 , B05D3/00 , C12M1/34
CPC分类号： C07K1/047 , B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y30/00 , C07B2200/11 , C07H21/00 , C40B40/06 , C40B40/10 , C40B60/14
摘要： The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture of polymer arrays.
摘要翻译：本发明提供了用于大规模制备聚合物序列阵列的新方法，其中每个阵列包括具有已知单体序列的多个不同的位置不同的聚合物序列。本发明的方法将高通量处理步骤与高分辨率光刻技术结合在聚合物阵列的制造中。

9. 发明申请

US20060147985A1 Methods and compositions for monitoring polymer array synthesis 审中-公开
标题翻译：监测聚合物阵列合成的方法和组成
公开(公告)号：US20060147985A1
公开(公告)日：2006-07-06
申请号：US11369628
申请日：2006-03-07
申请人： Anthony Barone , Glenn McGall , Evelyn Chai , Nam Ngo
发明人： Anthony Barone , Glenn McGall , Evelyn Chai , Nam Ngo
IPC分类号： C40B40/08 , C12Q1/68 , C07H21/04
CPC分类号： G01N33/54366 , C07H21/04 , C12Q1/6837 , C12Q2565/137 , C12Q2545/114 , C12Q2523/107
摘要： VLSIPS™ manufacturing processes are of increasing commercial importance. The present invention provides methods and compositions for monitoring the efficiency and quality of polymer synthesis in VLSIPS™ arrays. Methods for monitoring polymer synthesis in an array on a substrate are provided. Mono-isomeric labels for the labeling of synthetic polymer arrays are provided. Methods and compositions for post-synthetically labeling polymers in polymer arrays are also provided.
摘要翻译： VLSIPS（TM）制造工艺在商业上的重要性日益增加。本发明提供了用于监测VLSIPS TM阵列中聚合物合成的效率和质量的方法和组合物。提供了在基板上阵列中监测聚合物合成的方法。提供了用于标记合成聚合物阵列的单异构体标记。还提供了聚合物阵列中后合成标记聚合物的方法和组合物。

10. 发明申请

US20050181431A1 Substrate preparation process 审中-公开
公开(公告)号：US20050181431A1
公开(公告)日：2005-08-18
申请号：US11090876
申请日：2005-03-25
申请人： Martin Goldberg , Martin Diggelman , Earl Hubbell , Glenn McGall , Nam Ngo , MacDonald Morris , Mel Yamamoto , Jennifer Tan , Richard Rava
发明人： Martin Goldberg , Martin Diggelman , Earl Hubbell , Glenn McGall , Nam Ngo , MacDonald Morris , Mel Yamamoto , Jennifer Tan , Richard Rava
IPC分类号： B01J19/00 , C07B61/00 , C07H21/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B60/14 , C12Q1/68 , B05D3/00 , C12M1/34 , G01N33/53
CPC分类号： C07K1/047 , B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y30/00 , C07B2200/11 , C07H21/00 , C40B40/06 , C40B40/10 , C40B60/14
摘要： The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture of polymer arrays.

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