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1. 发明授权

US09089843B2 Fluid controlling apparatus and filter and biochip including the fluid controlling apparatus 有权
标题翻译：流体控制装置和过滤器以及包括流体控制装置的生物芯片
公开(公告)号：US09089843B2
公开(公告)日：2015-07-28
申请号：US13584552
申请日：2012-08-13
申请人： Minseok S. Kim , Sun-soo Kim , Jin-hoon Kim , Won-ho Lee , Jeong-gun Lee , Tae-seok Sim , Sang-hyun Baek , Hyo-young Jeong
发明人： Minseok S. Kim , Sun-soo Kim , Jin-hoon Kim , Won-ho Lee , Jeong-gun Lee , Tae-seok Sim , Sang-hyun Baek , Hyo-young Jeong
IPC分类号： B01D29/50 , C40B40/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/00 , B01L3/00 , G01N21/05 , G01N21/03 , G01N1/40
CPC分类号： B01L3/502753 , B01L3/502746 , B01L2200/10 , B01L2300/0816 , B01L2300/0819 , B01L2300/0877 , B01L2300/0887 , B01L2400/0487 , B01L2400/086 , G01N21/05 , G01N2001/4088 , G01N2021/0325 , G01N2021/0346
摘要： A fluid controlling apparatus including an inlet through which a fluid is introduced, a channel portion connected to the inlet, an outlet that is connected to the channel portion and through which the fluid is discharged, and at least one fluid resisting portion disposed between the inlet and the outlet, as well as a filter and a biochip including the fluid controlling apparatus.
摘要翻译：一种流体控制装置，其包括入口，流体通过该入口，与入口连接的通道部分，连接到通道部分并且流体被排出的出口以及设置在入口之间的至少一个流体阻力部分和出口，以及包括流体控制装置的过滤器和生物芯片。

2. 发明授权

US09421543B2 Hydrodynamic filter unit, hydrodynamic filter including the hydrodynamic filter unit, and method of filtering target material by using the hydrodynamic filter unit and the hydrodynamic filter 有权
标题翻译：流体动力过滤器单元，包括流体动力过滤器单元的流体动力过滤器，以及通过使用流体动力过滤器单元和流体动力过滤器过滤目标材料的方法
公开(公告)号：US09421543B2
公开(公告)日：2016-08-23
申请号：US13434252
申请日：2012-03-29
申请人： Minseok S. Kim , Won-ho Lee , Sun-soo Kim , Jin-hoon Kim , Jeong-gun Lee , Tae-seok Sim
发明人： Minseok S. Kim , Won-ho Lee , Sun-soo Kim , Jin-hoon Kim , Jeong-gun Lee , Tae-seok Sim
IPC分类号： B01D33/00 , B01D15/00 , B01D33/41 , B01D37/00 , B82Y99/00 , B01L3/00
CPC分类号： B01L3/502753 , B01L3/502761 , B01L2200/0652 , B01L2300/0816 , B01L2400/086
摘要： A hydrodynamic filter unit includes an inlet channel connected to a fluid chamber, into which a fluid including a target material is introduced, and a plurality of outlet channels connected to the fluid chamber, through which the fluid is discharged. A filtering method includes introducing a fluid including a target material into the hydrodynamic filter unit through the inlet channel, capturing the target material in the hydrodynamic filter unit, and discharging a remaining part of the fluid outside of the hydrodynamic filter unit through an outlet channel.
摘要翻译：流体动力过滤器单元包括连接到流体室的入口通道，包括目标材料的流体被引入到该流体室中，以及连接到流体室的多个出口通道，流体被排出。过滤方法包括通过入口通道将包括目标材料的流体引入到流体动力过滤器单元中，捕获流体动力过滤器单元中的目标材料，以及通过出口通道将流体动力过滤器单元外剩余部分排出。

3. 发明授权

US09238225B2 Fluid controlling apparatus and method of controlling fluid by using the same 有权
标题翻译：流体控制装置及其使用方法
公开(公告)号：US09238225B2
公开(公告)日：2016-01-19
申请号：US13571202
申请日：2012-08-09
申请人： June-young Lee , Sang-hyun Baek , Hyo-young Jeong , Tae-seok Sim , Jeong-gun Lee
发明人： June-young Lee , Sang-hyun Baek , Hyo-young Jeong , Tae-seok Sim , Jeong-gun Lee
IPC分类号： G01N1/20 , B01L3/00 , G01N35/00 , G01N35/04
CPC分类号： B01L3/502753 , B01L3/5027 , B01L2200/0621 , B01L2200/141 , B01L2300/0681 , B01L2300/0861 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , G01N2035/00237 , G01N2035/0437 , Y10T137/4259
摘要： A fluid controlling apparatus including at least one sample chamber for holding a fluid containing target materials; a cleaning chamber for holding a cleaning solution; a first multi-port connected to the at least one sample chamber through a first channel and connected to the cleaning chamber through a second channel; a filter portion, connected to the first multi-port through a third channel, for filtering the target materials; and a first pump, connected to the filter portion, for applying a pressure; and a method of controlling a fluid using the fluid controlling apparatus, which comprises passing the fluid containing the target materials from the at least one sample chamber to the filter portion; and cleaning a path of the fluid by passing the cleaning solution through the path.
摘要翻译：一种流体控制装置，包括用于保持含有目标材料的流体的至少一个样品室; 用于保持清洁溶液的清洁室; 第一多端口，其通过第一通道连接到所述至少一个样品室，并通过第二通道连接到所述清洁室; 过滤器部分，通过第三通道连接到第一多端口，用于过滤目标材料; 以及连接到过滤器部分的用于施加压力的第一泵; 以及使用所述流体控制装置控制流体的方法，所述方法包括将包含所述目标材料的流体从所述至少一个样品室传送到所述过滤器部分; 以及通过使清洁溶液通过路径来清洁流体的路径。

4. 发明授权

US08590710B2 Target particles-separating device and method using multi-orifice flow fractionation channel 有权
$Target particles-separating device and method using multi-orifice flow fractionation channel$
标题翻译：目标颗粒分离装置和使用多孔流量分馏通道的方法
公开(公告)号：US08590710B2
公开(公告)日：2013-11-26
申请号：US13156630
申请日：2011-06-09
申请人： Tae-seok Sim , Jeong-gun Lee , Hyo-il Jung , Ki-ho Kwon
发明人： Tae-seok Sim , Jeong-gun Lee , Hyo-il Jung , Ki-ho Kwon
IPC分类号： B07C5/00
CPC分类号： B01L3/502761 , B01L3/502753 , B01L2200/0652 , B01L2300/087 , B01L2400/0487
摘要： A device separating target particles in a fluid sample includes first through third multi-orifice flow fractionation (“MOFF”) channels, each including a multi-orifice segment with an inlet and an outlet at opposite ends, and an alternating series of contraction channels and expansion chambers interconnected in a lengthwise direction; a first separation unit including a first separation channel which is interconnected in fluid communication with a center region of the outlet of the first MOFF channel, and first branch channels which are interconnected in fluid communication with sidewall regions of the outlet of the first MOFF channel, and respectively with inlets of the second and third MOFF channels; and buffer inlets which are connected to the inlets of the second and third MOFF channels and through which a buffer flows into the second and third MOFF channels.
摘要翻译：分离流体样品中的目标颗粒的装置包括第一至第三多孔流动分馏（“MOFF”）通道，每个通道包括具有相对端的入口和出口的多孔段，以及交替的一系列收缩通道和膨胀室沿长度方向互相连接; 第一分离单元，其包括与第一MOFF通道的出口的中心区域流体连通的第一分离通道和与第一MOFF通道的出口的侧壁区域流体连通地互连的第一分支通道，并分别具有第二和第三MOFF通道的入口; 以及连接到第二和第三MOFF通道的入口并且缓冲器通过其流入第二和第三MOFF通道的缓冲器入口。

5. 发明授权

US08993340B2 Device and method of separating cells by using magnetic force 有权
标题翻译：使用磁力分离细胞的装置和方法
公开(公告)号：US08993340B2
公开(公告)日：2015-03-31
申请号：US13051251
申请日：2011-03-18
申请人： Hun-joo Lee , Jeong-gun Lee , Mi-jeong Song , Jong-myeon Park , Tae-seok Sim , Min-seok S. Kim
发明人： Hun-joo Lee , Jeong-gun Lee , Mi-jeong Song , Jong-myeon Park , Tae-seok Sim , Min-seok S. Kim
IPC分类号： G01N1/28 , C12N5/00 , C12M1/00 , B01L3/00 , B03C1/14 , G01N1/40 , C12M1/26 , B03C1/02
CPC分类号： C12M47/04 , B01L3/502753 , B01L3/502761 , B01L2300/0864 , B03C1/02 , B03C1/14 , B03C1/145 , C12M1/266 , G01N1/4077 , Y10T436/25
摘要： A cell separation device includes a channel or chamber in which a sample flows or moves, the sample including target cells marked with magnetic particles, and non-target cells, and a magnet which generates a magnetic first force in a first direction with respect to the sample within the channel or chamber. The channel or chamber of the cell separation device is applied with a second force in a second direction opposite to the first direction of the magnetic force. According to the cell separation device and a method of separating cells, the target cells move in the first direction by the magnetic force, and the non-target cells move in the second direction by the second force, by simultaneously applying the magnetic force and the second force in opposing directions, thereby separating the target cells from the non-target cells.
摘要翻译：细胞分离装置包括样品流动或移动的通道或室，包括标记有磁性颗粒的靶细胞的样品和非靶细胞，以及相对于第一方向产生第一方向的磁性第一力的磁体样品在通道或室内。细胞分离装置的通道或腔室以与磁力的第一方向相反的第二方向施加第二力。根据细胞分离装置和分离细胞的方法，靶细胞通过磁力在第一方向上移动，非靶细胞通过第二力在第二方向上移动，同时施加磁力和从而将目标细胞与非靶细胞分离。

6. 发明授权

US08846322B2 Method and kit for isolating target cell 有权
标题翻译：用于分离靶细胞的方法和试剂盒
公开(公告)号：US08846322B2
公开(公告)日：2014-09-30
申请号：US13582931
申请日：2011-03-07
申请人： Jong-myeon Park , Jeong-gun Lee
发明人： Jong-myeon Park , Jeong-gun Lee
IPC分类号： G01N33/53 , G01N33/574 , G01N33/543 , G01N33/569
CPC分类号： G01N33/57492 , C07K16/30 , C07K17/08 , G01N33/543 , G01N33/54313 , G01N33/54353 , G01N33/56966 , G01N33/574 , G01N33/57484 , Y10T428/2982
摘要： Provided are a method and kit for isolating a target cell by using particles comprising polymers having positive charge and negative charge and at least one antibody bound to polymers. The target cell in the biological sample may be efficiently isolated and detected by using a method and kit according to an exemplary embodiment.
摘要翻译：提供了通过使用包含具有正电荷和负电荷的聚合物的颗粒和与聚合物结合的至少一种抗体来分离靶细胞的方法和试剂盒。通过使用根据示例性实施方案的方法和试剂盒可以有效地分离和检测生物样品中的靶细胞。

7. 发明申请

US20130052664A1 METHOD AND KIT FOR ISOLATING TARGET CELL 有权
标题翻译：用于分离目标细胞的方法和工具包
公开(公告)号：US20130052664A1
公开(公告)日：2013-02-28
申请号：US13582931
申请日：2011-03-07
申请人： Jong-myeon Park , Jeong-gun Lee
发明人： Jong-myeon Park , Jeong-gun Lee
IPC分类号： G01N33/574 , C08G73/04 , C08F12/08 , C08F228/02 , G01N33/566 , C12M1/34
CPC分类号： G01N33/57492 , C07K16/30 , C07K17/08 , G01N33/543 , G01N33/54313 , G01N33/54353 , G01N33/56966 , G01N33/574 , G01N33/57484 , Y10T428/2982
摘要： Provided are a method and kit for isolating a target cell by using particles comprising polymers having positive charge and negative charge and at least one antibody bound to polymers. The target cell in the biological sample may be efficiently isolated and detected by using a method and kit according to an exemplary embodiment.
摘要翻译：提供了通过使用包含具有正电荷和负电荷的聚合物的颗粒和与聚合物结合的至少一种抗体来分离靶细胞的方法和试剂盒。通过使用根据示例性实施方案的方法和试剂盒可以有效地分离和检测生物样品中的靶细胞。

8. 发明授权

US08252569B2 Microfluidic device and method for concentration and lysis of cells or viruses 有权
标题翻译：用于细胞或病毒浓缩和溶解的微流控装置和方法
公开(公告)号：US08252569B2
公开(公告)日：2012-08-28
申请号：US13034857
申请日：2011-02-25
申请人： Yoon-kyoung Cho , Jeong-gun Lee , Sung-young Jeong
发明人： Yoon-kyoung Cho , Jeong-gun Lee , Sung-young Jeong
IPC分类号： C12N13/00 , C12N1/08 , G01N33/00
CPC分类号： B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L2200/10 , B01L2300/1861 , B01L2400/0421 , B01L2400/0424 , B01L2400/043 , B01L2400/0439 , C12M47/02 , C12M47/04 , C12M47/06 , Y10T436/25 , Y10T436/2575
摘要： A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.
摘要翻译：用于细胞或病毒的浓缩和裂解的微流体装置以及使用微流体装置浓缩和裂解细胞或病毒的方法包括：磁珠，容纳磁珠的反应室和激光源。反应室包括彼此交叉并被电介质隔开以产生电场的多个电极以及搅拌室中的磁珠的振动部分。激光源将激光辐射到反应室中的磁珠上。

9. 发明授权

US08057759B2 Microfluidic system and apparatus and method of controlling the same 有权
标题翻译：微流体系统及其控制方法
公开(公告)号：US08057759B2
公开(公告)日：2011-11-15
申请号：US11835461
申请日：2007-08-08
申请人： Jeong-gun Lee , Yoon-kyoung Cho , Beom-seok Lee , Jong-myeon Park
发明人： Jeong-gun Lee , Yoon-kyoung Cho , Beom-seok Lee , Jong-myeon Park
IPC分类号： B01L3/02 , B01L3/00 , G01N15/06 , G01N33/00
CPC分类号： B01L3/502715 , B01L3/5027 , B01L3/50273 , B01L3/502738 , B01L2200/14 , B01L2300/0806 , B01L2300/1861 , B01L2400/0409 , B01L2400/0677 , F16K99/0001 , F16K99/0032 , F16K2099/0084 , F16K2099/0086 , G01N35/00069 , Y10T436/11 , Y10T436/25375 , Y10T436/2575
摘要： Provided are an apparatus and a method of controlling a microfluidic system, and the microfluidic system. The apparatus of controlling the microfluidic system includes a central control block controlling an operation of the microfluidic system, a rotator control block controlling a rotator, a position control block controlling the position of a moving unit, the moving unit moving to a position of the microfluidic structure, and a radiation energy source control block controlling energy of a radiation energy source, the radiation energy source using an electromagnetic wave to scan over a position of the microfluidic structure. Such a configuration allows effective control of a miniaturized portable microfluidic system.
摘要翻译：提供了一种控制微流体系统和微流体系统的装置和方法。控制微流体系统的装置包括控制微流体系统的操作的中央控制块，控制旋转器的旋转体控制块，控制移动单元的位置的位置控制块，移动到微流体位置的移动单元结构以及辐射能量源控制块，其控制辐射能源的能量，所述辐射能源使用电磁波扫描微流体结构的位置。这种配置允许有效地控制微型便携式微流体系统。

10. 发明授权

US07959862B2 Microfluidic device and method for concentration and lysis of cells or viruses 有权
标题翻译：用于细胞或病毒浓缩和溶解的微流控装置和方法
公开(公告)号：US07959862B2
公开(公告)日：2011-06-14
申请号：US11610938
申请日：2006-12-14
申请人： Yoon-kyoung Cho , Jeong-gun Lee , Sung-young Jeong
发明人： Yoon-kyoung Cho , Jeong-gun Lee , Sung-young Jeong
IPC分类号： G01N15/06
CPC分类号： B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L2200/10 , B01L2300/1861 , B01L2400/0421 , B01L2400/0424 , B01L2400/043 , B01L2400/0439 , C12M47/02 , C12M47/04 , C12M47/06 , Y10T436/25 , Y10T436/2575
摘要： A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.
摘要翻译：用于细胞或病毒的浓缩和裂解的微流体装置以及使用微流体装置浓缩和裂解细胞或病毒的方法包括：磁珠，容纳磁珠的反应室和激光源。反应室包括彼此交叉并被电介质隔开以产生电场的多个电极以及搅拌室中的磁珠的振动部分。激光源将激光辐射到反应室中的磁珠上。

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