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1. 发明授权

US5879686A Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis 失效
公开(公告)号：US5879686A
公开(公告)日：1999-03-09
申请号：US853504
申请日：1997-05-08
申请人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
发明人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
IPC分类号： C12N15/09 , A61K39/00 , A61K39/095 , A61P31/04 , C07K1/18 , C07K14/01 , C07K14/22 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/36
CPC分类号： C07K14/005 , C07K14/22 , C12N15/70 , A61K39/00 , C07K2319/00 , C12N2795/10022 , Y10S435/849
摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

2. 发明授权

US5747287A Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis 失效
公开(公告)号：US5747287A
公开(公告)日：1998-05-05
申请号：US877109
申请日：1997-06-17
申请人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
发明人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
IPC分类号： A61K39/00 , C07K14/01 , C07K14/22 , C07K1/00 , A61K39/05 , C12N15/00 , C12P21/06
CPC分类号： C07K14/005 , C07K14/22 , A61K39/00 , C07K2319/00 , C12N2795/10022
摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

3. 发明授权

US5439808A Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis 失效
标题翻译：脑膜炎奈瑟氏球菌外膜组B孔蛋白的高水平表达，纯化和重折叠的方法
公开(公告)号：US5439808A
公开(公告)日：1995-08-08
申请号：US96182
申请日：1993-07-23
申请人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
发明人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
IPC分类号： C12N15/09 , A61K39/00 , A61K39/095 , A61P31/04 , C07K1/18 , C07K14/01 , C07K14/22 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/36 , C12N15/62 , C12N15/67
CPC分类号： C07K14/005 , C07K14/22 , C12N15/70 , A61K39/00 , C07K2319/00 , C12N2795/10022 , Y10S435/849
摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins yard fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.
摘要翻译：本发明一般涉及外膜蛋白脑膜炎球菌B族孔蛋白及其融合蛋白的高水平表达的方法。特别地，本发明涉及在大肠杆菌中表达外膜蛋白质脑膜炎双球菌B蛋白质的蛋白质的方法，其中脑膜炎球菌B蛋白质组蛋白质的蛋白质融合蛋白质包含在大肠杆菌中表达的总蛋白的2％以上。本发明还涉及脑膜炎球菌B族孔蛋白及其融合蛋白的纯化和重折叠方法及其在疫苗中的应用。

4. 发明授权

US6013267A Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis 失效
标题翻译：脑膜炎奈瑟氏球菌外膜组B孔蛋白的高水平表达，纯化和重折叠的方法
公开(公告)号：US6013267A
公开(公告)日：2000-01-11
申请号：US798760
申请日：1997-02-11
申请人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J.J. Hronowski , Jeffrey K. Pullen
发明人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J.J. Hronowski , Jeffrey K. Pullen
IPC分类号： C12N15/09 , A61K39/00 , A61K39/095 , A61P31/04 , C07K1/18 , C07K14/01 , C07K14/22 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/36
CPC分类号： C07K14/005 , C07K14/22 , C12N15/70 , A61K39/00 , C07K2319/00 , C12N2795/10022 , Y10S435/849
摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.
摘要翻译：本发明一般涉及外膜蛋白脑膜炎球菌B族孔蛋白及其融合蛋白的高水平表达的方法。特别地，本发明涉及一种在大肠杆菌中表达外膜蛋白质脑膜炎球菌B蛋白孔蛋白的方法，其中脑膜炎球菌B族孔蛋白及其融合蛋白包含大肠杆菌中表达的总蛋白的2％以上。本发明还涉及脑膜炎球菌B族孔蛋白及其融合蛋白的纯化和重折叠方法及其在疫苗中的应用。

5. 发明授权

US6153406A Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B 失效
标题翻译：来自流感嗜血杆菌B型的外膜蛋白P2的高水平表达，纯化和重折叠的方法
公开(公告)号：US6153406A
公开(公告)日：2000-11-28
申请号：US96181
申请日：1993-07-23
申请人： Joseph Y. Tai , Jeffrey K. Pullen , Thomas Soper , Shu-Mei Liang , Milan S. Blake
发明人： Joseph Y. Tai , Jeffrey K. Pullen , Thomas Soper , Shu-Mei Liang , Milan S. Blake
IPC分类号： C12N15/09 , A61K39/00 , A61K39/102 , A61P31/04 , C07K1/34 , C07K14/01 , C07K14/11 , C07K14/285 , C07K16/10 , C12N1/21 , C12P21/02 , C12P21/04 , C12R1/19 , C12R1/92 , C12N1/20
CPC分类号： C07K14/005 , C07K14/285 , A61K39/00 , C07K2319/00 , C12N2795/10222
摘要： The present invention relates, in general, to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b (Hib-P2) and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b or fusion protein thereof in E. coli wherein the Hib-P2 protein or fusion protein comprises more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of Hib-P2 protein and fusion protein thereof.
摘要翻译：本发明一般涉及从流感嗜血杆菌b型（Hib-P2）表达外膜蛋白P2及其融合蛋白的方法。特别地，本发明涉及从流感嗜血杆菌b型或其融合蛋白的大肠杆菌中表达外膜蛋白P2的方法，其中Hib-P2蛋白或融合蛋白包含超过总蛋白的2％大肠杆菌本发明还涉及Hib-P2蛋白及其融合蛋白的纯化和重折叠方法。

6. 发明授权

US06764686B2 Modified immunogenic pneumolysin compositions as vaccines 失效
标题翻译：修饰的免疫原性肺炎溶蛋白组合物作为疫苗
公开(公告)号：US06764686B2
公开(公告)日：2004-07-20
申请号：US09120044
申请日：1998-07-21
申请人： Conceicao Minetti , Francis Michon , Jeffrey K. Pullen , Mary Ellen Polvino-Bodnar , Shu-Mei Liang , Joseph Y. Tai
发明人： Conceicao Minetti , Francis Michon , Jeffrey K. Pullen , Mary Ellen Polvino-Bodnar , Shu-Mei Liang , Joseph Y. Tai
IPC分类号： A61K3902
CPC分类号： C07K14/3156 , A61K39/092 , A61K2039/6037 , Y10S424/831 , Y10S530/825
摘要： This invention relates to modified pneumolysin polypeptides that retain the immunogenic nature of pneumolysin but have reduced or undetectable hemolytic activity compared to native pneumolysin. The invention also provides a method for generating novel pneumolysin variants with these desired characteristic properties. The invention also provides immunogenic compositions useful as pharmaceutical compositions including vaccines in which non-toxic, modified pneumolysin is used to stimulate protective immunity against Streptococcus pneumoniae. The vaccines may be compositions in which the modified pneumolysin is conjugated to bacterial polysaccharides or may be carried on an attenuated viral vector. In addition, the invention also provides a method of using the non-toxic, modified pneumolysin toxoid in order to stimulate antibodies against Streptococcus pneumoniae in a treated individual which are then isolated and transferred to a second individual, thereby conferring protection against Streptococcus pneumoniae in the second individual.
摘要翻译：本发明涉及与天然气溶素相比，保留免疫原性的气溶菌素的修饰的气溶胶素多肽，但具有降低的或不可检测的溶血活性。本发明还提供了一种产生具有这些所需特征性质的新型肺炎球菌变异体的方法。本发明还提供可用作药物组合物的免疫原性组合物，包括其中无毒的改良的肺炎溶血杆菌刺激对肺炎链球菌的保护性免疫的疫苗。疫苗可以是其中改性气溶胶与细菌多糖缀合或可以携带在减毒病毒载体上的组合物。此外，本发明还提供了一种使用无毒的改良型肺炎杆菌素类毒素以刺激处理的个体中针对肺炎链球菌的抗体的方法，然后将其分离并转移到第二个体中，从而在肺炎链球菌中得到保护第二个人。

7. 发明授权

US08071105B2 Reishi F3 sub fraction polysaccharides and methods of using same 有权
$Reishi F3 sub fraction polysaccharides and methods of using same$
标题翻译： Reishi F3分级多糖及其使用方法
公开(公告)号：US08071105B2
公开(公告)日：2011-12-06
申请号：US12244709
申请日：2008-10-02
申请人： Shu-Mei Liang , Yen-Po Chen , Wen-Bin Yang , Chi-Huey Wong
发明人： Shu-Mei Liang , Yen-Po Chen , Wen-Bin Yang , Chi-Huey Wong
IPC分类号： A61K36/06
CPC分类号： A61K39/0002 , A61K2039/55588
摘要： The present disclosure relates to the discovery of methods of isolating subfractions of an F3 Reishi extract, and of administration of these novel isolates to eukaryotic cells in order to induce certain immumodulatory, hematopoeitic and tumor-inhibiting phenotypic changes in those eukaryotic cells, mediated through particular toll-like receptor (TLR) and other transmembrane receptors. F3 subfractions F301 and F331 have demonstrated that F331 is capable of activating at least TLR-2 while F301 is capable of activating at least TLR-2, TLR-4, and TLR-5.
摘要翻译：本公开涉及发现分离F3赖氏提取物的亚组分的方法，以及将这些新型分离物施用于真核细胞，以便诱导那些通过特异性介导的那些真核细胞中的某些免疫调节，造血细胞和肿瘤抑制表型变化 toll样受体（TLR）和其他跨膜受体。 F3亚分数F301和F331已经证明F331能够至少激活至少TLR-2，而F301能够至少激活TLR-2，TLR-4和TLR-5。

8. 发明申请

US20100183649A1 METHOD OF PRODUCING VIRUS-LIKE PARTICLES OF PICORNAVIRUS USING A SMALL-UBIQUITIN-RELATED FUSION PROTEIN EXPRESSION SYSTEM 有权
标题翻译：使用与小蛋白质相关的融合蛋白表达系统生产拟南芥的病毒颗粒的方法
公开(公告)号：US20100183649A1
公开(公告)日：2010-07-22
申请号：US12683568
申请日：2010-01-07
申请人： Ting-Fang Wang , Shu-Mei Liang
发明人： Ting-Fang Wang , Shu-Mei Liang
IPC分类号： A61K39/135 , C12P21/00 , C12P21/06 , A61P31/12 , C12N1/20
CPC分类号： A61K39/135 , A61K39/12 , A61K2039/5258 , A61K2039/552 , C07K2319/21 , C07K2319/35 , C07K2319/95 , C12N7/00 , C12N2770/32123 , C12N2770/32134
摘要： A method for producing picornaviral capsid protein complexes (e.g., picornavirus like particles) in E. coli using a small-ubiquitin-related fusion protein expression system and an E. coli strain used in practicing this method. Also disclosed is use of the picornaviral capsid protein complexes like thus prepared for eliciting immune responses.
摘要翻译：使用小泛素相关融合蛋白表达系统和用于实施该方法的大肠杆菌菌株在大肠杆菌中生产小核糖核酸病毒衣壳蛋白复合物（例如，小核糖核酸病毒样颗粒）的方法。还公开了如此制备的小核糖核酸病毒衣壳蛋白复合物用于引发免疫应答。

9. 发明授权

US07323546B2 Apoptosis-inducing polypeptides 有权
标题翻译：细胞凋亡诱导多肽
公开(公告)号：US07323546B2
公开(公告)日：2008-01-29
申请号：US10863637
申请日：2004-06-08
申请人： Shu-Mei Liang , Jei-Ming Peng , Chi-Ming Liang
发明人： Shu-Mei Liang , Jei-Ming Peng , Chi-Ming Liang
IPC分类号： C07K14/00 , A61K39/00
CPC分类号： C07K14/005 , A61K31/7088 , A61K38/162 , A61K39/00 , A61K39/12 , A61K39/135 , A61K2039/523 , C07K1/1136 , C12N2770/32122 , C12N2770/32133
摘要： An isolated water-soluble VP1 polypeptide of foot-and-mouth disease virus and a nucleic acid encoding the polypeptide. Also disclosed are a pharmaceutical composition containing the polypeptide or nucleic acid and related methods of inducing apoptosis and treating an apoptosis-related disorder.
摘要翻译：口蹄疫病毒的分离的水溶性VP1多肽和编码该多肽的核酸。还公开了含有多肽或核酸的药物组合物和诱导凋亡和治疗凋亡相关病症的相关方法。

10. 发明授权

US08357652B2 Anti-tumor fibrillar human serum albumin methods and compositions 有权
标题翻译：抗肿瘤原纤维人血清白蛋白的方法和组成
公开(公告)号：US08357652B2
公开(公告)日：2013-01-22
申请号：US12623162
申请日：2009-11-20
申请人： Shu-Mei Liang , Chi-Ming Liang
发明人： Shu-Mei Liang , Chi-Ming Liang
IPC分类号： A61K35/12 , A61K38/00
CPC分类号： A61K38/385 , A61K38/162 , A61K38/38 , A61K45/06 , A61K47/643 , A61N5/10 , C07K14/765 , C12N7/00 , C12N2770/32133
摘要： Fibrillar human serum albumin was shown to be effective in the treatment of various types of cancers. Methods and compositions are disclosed for using fibrillar human serum albumin as a medicament to treat subjects having cancer.
摘要翻译：纤维素人血清白蛋白显示有效治疗各种类型的癌症。公开了使用原纤维人血清白蛋白作为治疗患有癌症的受试者的药物的方法和组合物。

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