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1. 发明授权

US5879686A Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis 失效
公开(公告)号：US5879686A
公开(公告)日：1999-03-09
申请号：US853504
申请日：1997-05-08
申请人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
发明人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
IPC分类号： C12N15/09 , A61K39/00 , A61K39/095 , A61P31/04 , C07K1/18 , C07K14/01 , C07K14/22 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/36
CPC分类号： C07K14/005 , C07K14/22 , C12N15/70 , A61K39/00 , C07K2319/00 , C12N2795/10022 , Y10S435/849
摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

2. 发明授权

US08906421B2 Methods of dissolving blood clots and the like 有权
标题翻译：溶血栓等的方法
公开(公告)号：US08906421B2
公开(公告)日：2014-12-09
申请号：US13529445
申请日：2012-06-21
申请人： John S. Fisher , Fred Ahari , Christopher Jon Perry , Lucjan J. J. Hronowski
发明人： John S. Fisher , Fred Ahari , Christopher Jon Perry , Lucjan J. J. Hronowski
IPC分类号： A61K33/00 , A61K31/17 , A61K31/16 , A61K31/155 , A61K33/06 , A61P7/02 , A61K47/18 , A61K31/164 , A61K9/00 , A61K31/198
CPC分类号： A61K31/164 , A61K9/0019 , A61K31/17 , A61K31/198 , A61K33/00 , A61K47/183 , A61K2300/00
摘要： The present invention provides an aqueous composition and its use for dissolving blood clots in vivo or in clinical or research samples in vitro, said composition comprising at least one chaotropic agent, at least one chelating agent that complexes iron if the chaotropic agent does not complex iron and at least one surface-active emulsifier, the composition being buffered near pH 7.4. The process of dissolving the clot with the aqueous composition is advantageous because it is simple, inexpensive, rapid and can be applied in a variety of contexts, including basic research and clinical situations.
摘要翻译：本发明提供一种水性组合物及其在体内或体外临床或研究样品中溶血栓的用途，所述组合物包含至少一种离液剂，至少一种螯合剂，如果离液剂不复合铁，则络合铁和至少一种表面活性乳化剂，该组合物在pH 7.4附近缓冲。使用水性组合物溶解凝块的过程是有利的，因为其简单，便宜，快速，并且可以应用于各种情况，包括基础研究和临床情况。

3. 发明授权

US5439808A Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis 失效
标题翻译：脑膜炎奈瑟氏球菌外膜组B孔蛋白的高水平表达，纯化和重折叠的方法
公开(公告)号：US5439808A
公开(公告)日：1995-08-08
申请号：US96182
申请日：1993-07-23
申请人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
发明人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
IPC分类号： C12N15/09 , A61K39/00 , A61K39/095 , A61P31/04 , C07K1/18 , C07K14/01 , C07K14/22 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/36 , C12N15/62 , C12N15/67
CPC分类号： C07K14/005 , C07K14/22 , C12N15/70 , A61K39/00 , C07K2319/00 , C12N2795/10022 , Y10S435/849
摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins yard fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.
摘要翻译：本发明一般涉及外膜蛋白脑膜炎球菌B族孔蛋白及其融合蛋白的高水平表达的方法。特别地，本发明涉及在大肠杆菌中表达外膜蛋白质脑膜炎双球菌B蛋白质的蛋白质的方法，其中脑膜炎球菌B蛋白质组蛋白质的蛋白质融合蛋白质包含在大肠杆菌中表达的总蛋白的2％以上。本发明还涉及脑膜炎球菌B族孔蛋白及其融合蛋白的纯化和重折叠方法及其在疫苗中的应用。

4. 发明授权

US5425946A Vaccines against group C Neisseria meningitidis 失效
标题翻译：疫苗针对C组脑膜炎奈瑟氏球菌
公开(公告)号：US5425946A
公开(公告)日：1995-06-20
申请号：US938367
申请日：1992-08-31
申请人： Joseph Y. Tai , Lucjan J. J. Hronowski , Sharon Mates
发明人： Joseph Y. Tai , Lucjan J. J. Hronowski , Sharon Mates
IPC分类号： A61K39/095 , G01N33/68 , C07K1/10
CPC分类号： A61K39/095 , G01N33/6854 , A61K2039/6037 , Y10S530/807
摘要： An immunogenic conjugate comprising a modified group C meningococcal polysaccharide (GCMP) coupled to a carrier molecule. The GCMP is modified by O-deacetylation to a varying extent. This modified GCMP conjugate stimulates a strong immunogenic response. The antiserum, produced by immunization with this conjugate, is cross-reactive with the native polysaccharide. In addition, these antisera elicit a class of antibodies that have patent bactericidal activity.
摘要翻译：包含与载体分子偶联的修饰的C组脑膜炎球菌多糖（GCMP）的免疫原性缀合物。通过O-去乙酰化可以改变GCMP的程度。该修饰的GCMP缀合物刺激强的免疫原性反应。通过用该缀合物免疫产生的抗血清与天然多糖交叉反应。此外，这些抗血清引起一类具有专利杀菌活性的抗体。

5. 发明授权

US07329414B2 Biodegradable polymer for marking tissue and sealing tracts 有权
标题翻译：用于标记组织和密封带的生物可降解聚合物
公开(公告)号：US07329414B2
公开(公告)日：2008-02-12
申请号：US10063620
申请日：2002-05-03
申请人： John S. Fisher , Frederick Ahari , Lucjan J. J. Hronowski
发明人： John S. Fisher , Frederick Ahari , Lucjan J. J. Hronowski
IPC分类号： A61F2/02 , A61K51/02
CPC分类号： A61L24/0042 , A61B90/39 , A61B2017/00004 , A61B2017/00893 , A61B2017/00898 , A61L24/0015 , A61L24/0031 , A61L31/18 , A61L2300/406 , A61L2300/41 , A61L2300/416 , A61L2300/44 , A61L2300/604
摘要： A tissue marker formed of a biodegradable polymer having drug-delivery capabilities is combined with a sealant that encapsulates the tissue marker and which serves to help anchor the tissue marker against migration. The sealant is delivered to a site in dehydrated form and moisture inherent in tissue at the site expands the sealant. The expanded sealant is formed of a hydrogel and is therefore more compatible to the surrounding tissue than the material of the tissue marker. The sealant and the tissue marker are both bioabsorbed over time.
摘要翻译：由具有药物递送能力的可生物降解的聚合物形成的组织标记物与封装组织标记物的密封剂组合，并且其用于帮助锚定组织标记物抵抗迁移。密封剂被递送到脱水形式的部位，并且在该部位组织中固有的水分使密封剂膨胀。膨胀的密封剂由水凝胶形成，因此与组织标记的材料相比，与周围组织更相容。密封剂和组织标记物随时间生物吸收。

6. 发明授权

US07001410B2 Bioabsorbable sealant 有权
公开(公告)号：US07001410B2
公开(公告)日：2006-02-21
申请号：US10249263
申请日：2003-03-27
申请人： John S. Fisher , Frederick Ahari , Lucjan J. J. Hronowski
发明人： John S. Fisher , Frederick Ahari , Lucjan J. J. Hronowski
IPC分类号： A61B17/04
CPC分类号： A61B17/0644 , A61B17/0057 , A61B17/064 , A61B2017/00004 , A61B2017/00575 , A61B2017/00601
摘要： Openings in a mammalian body made by any medical procedure or non-medical event are sealed with a bioabsorbable plug or sewn with a bioabsorbable suture. In one exemplary embodiment, the plug in dehydrated, unexpanded condition is pushed by a pushing device through the lumen of a needle until a first part of the plug is external to the opening and a second part is internal to the opening. The needle is then withdrawn while the position of the pushing device is maintained. The pushing device is then withdrawn, leaving the plug in sealing relation to the opening. The body's moisture causes the plug to expand to complete the sealing of the opening, or the expansion may be caused by exposure to air, light, or other stimulant. The opening may be formed in soft tissue, internal organs, or hard tissue. The plug seals the flow of liquid or gaseous biological fluids.

7. 发明授权

US06592608B2 Bioabsorbable sealant 失效
标题翻译：生物可吸收密封剂
公开(公告)号：US06592608B2
公开(公告)日：2003-07-15
申请号：US09683282
申请日：2001-12-07
申请人： John S. Fisher , Frederick Ahari , Lucjan J. J. Hronowski
发明人： John S. Fisher , Frederick Ahari , Lucjan J. J. Hronowski
IPC分类号： A61D100
CPC分类号： A61B17/0644 , A61B17/0057 , A61B17/064 , A61B2017/00004 , A61B2017/00575 , A61B2017/00601
摘要： Openings in a mammalian body made by any medical procedure or non-medical event are sealed with a bioabsorbable plug or sewn with a bioabsorbable suture. In one exemplary embodiment, the plug in dehydrated, unexpanded condition is pushed by a pushing device through the lumen of a needle until a first part of the plug is external to the opening and a second part is internal to the opening. The needle is then withdrawn while the position of the pushing device is maintained. The pushing device is then withdrawn, leaving the plug in sealing relation to the opening. The body's moisture causes the plug to expand to complete the sealing of the opening, or the expansion may be caused by exposure to air, light, or other stimulant. The opening may be formed in soft tissue, internal organs, or hard tissue. The plug seals the flow of liquid or gaseous biological fluids.
摘要翻译：通过任何医疗程序或非医疗事件制成的哺乳动物体内的开口用生物可吸收的塞子密封或用生物可吸收的缝线缝合。在一个示例性实施例中，脱水，未膨胀状态的塞子被推动装置推动穿过针管，直到插头的第一部分在开口外部，第二部分在开口内部。然后在保持推动装置的位置的同时取出针。然后将推动装置取出，使塞子与开口密封。身体的水分导致插头膨胀以完成开口的密封，或者膨胀可能由于暴露于空气，光线或其他兴奋剂而引起。开口可以形成在软组织，内部器官或硬组织中。塞子密封液体或气体生物流体的流动。

8. 发明授权

US5747287A Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis 失效
公开(公告)号：US5747287A
公开(公告)日：1998-05-05
申请号：US877109
申请日：1997-06-17
申请人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
发明人： Milan S. Blake , Joseph Y. Tai , Huilin L. Qi , Shu-Mei Liang , Lucjan J. J. Hronowski , Jeffrey K. Pullen
IPC分类号： A61K39/00 , C07K14/01 , C07K14/22 , C07K1/00 , A61K39/05 , C12N15/00 , C12P21/06
CPC分类号： C07K14/005 , C07K14/22 , A61K39/00 , C07K2319/00 , C12N2795/10022
摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

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