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1. 发明申请

US20090137053A1 BEAD SET, PRODUCTION PROCESS OF THE BEAD SET, AND METHOD OF USING THE BEAD SET 审中-公开
标题翻译：珠组，珠组的生产过程以及使用珠组的方法
公开(公告)号：US20090137053A1
公开(公告)日：2009-05-28
申请号：US12108348
申请日：2008-04-23
申请人： Noriyuki Kishii , Mari Ichimura , Tomoteru Abe , Kazuhiro Nakagawa , Akio Yasuda , Mayumi Shiono , Michihiro Ohnishi , Takuro Yamamoto , Minoru Takeda
发明人： Noriyuki Kishii , Mari Ichimura , Tomoteru Abe , Kazuhiro Nakagawa , Akio Yasuda , Mayumi Shiono , Michihiro Ohnishi , Takuro Yamamoto , Minoru Takeda
IPC分类号： G01N33/50 , B05D3/00
CPC分类号： G01N21/645 , C12Q1/6869 , G01N33/54313 , Y10T436/143333 , C12Q2563/149
摘要： A bead set is composed of plural subsets of beads. The beads in each subset are each provided with a surface permitting immobilization thereon of a substance that takes part in a predetermined corresponding reaction or interaction. The individual beads in the beat set are provided with different physical elements, respectively, such that the individual beads in the bead can be distinguished into the plural subsets through an analysis of captured images of the individual beads in the bead set by a computer.
摘要翻译：珠组由多个珠子组成。每个子集中的珠粒各自具有允许在其上固定参与预定相应反应或相互作用的物质的表面。节拍组中的各个珠分别设置有不同的物理元件，使得可以通过分析由计算机设置的珠粒中的各个珠子的捕获图像来将珠粒中的各个珠子区分为多个子集。

2. 发明授权

US08753898B2 Microbead analysis method and microbead analyzer 失效
标题翻译：微珠分析法和微珠分析仪
公开(公告)号：US08753898B2
公开(公告)日：2014-06-17
申请号：US12877237
申请日：2010-09-08
申请人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
发明人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
IPC分类号： G01N33/543
CPC分类号： G01N33/54366 , G01N21/6452
摘要： Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.
摘要翻译：微珠的微珠分析方法。微珠形成为具有彼此面对的顶表面和底表面的几乎平行的柱状和从其延伸的侧表面，并且携带形成在顶表面和底部中的至少一个上的识别图案表面和固定在其对分析物质具有亲和性的表面上的物质。该方法包括检测由于分析物质与对分析物质具有亲和性的物质的相互作用而从微珠表面发射的荧光，该区域包括不形成识别图案的顶表面和底表面的区域，侧面表面。

3. 发明授权

US08548221B2 Microbead automatic recognition method and microbead 有权
标题翻译：微珠自动识别方法和微珠
公开(公告)号：US08548221B2
公开(公告)日：2013-10-01
申请号：US13440366
申请日：2012-04-05
申请人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
发明人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
IPC分类号： G06K9/00
CPC分类号： G06K19/06037 , G06T7/74 , G06T2207/30072 , G06T2207/30208 , Y10T428/2991
摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.
摘要翻译：微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。

4. 发明授权

US08409706B2 Preparation process of microbeads, and microbeads 有权
标题翻译：微珠和微珠的制备过程
公开(公告)号：US08409706B2
公开(公告)日：2013-04-02
申请号：US12432108
申请日：2009-04-29
申请人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Masanobu Tanaka
发明人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Masanobu Tanaka
IPC分类号： B32B1/00 , G03F7/20
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00459 , B01J2219/00466 , B01J2219/005 , B01J2219/00502 , B01J2219/00542 , B01J2219/00547 , B01J2219/00556 , B01J2219/00558 , B01J2219/0056 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00617 , B01J2219/00619 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , Y10T428/2991
摘要： The present application provides a process for the preparation of microbeads, including the steps of: patterning a thin film, which has been formed on a substrate, into microbead regions of at least one predetermined shape by photolithography; immobilizing a predetermined substance on the thus-patterned microbead regions; and stripping off, from the substrate, the thus-patterned microbead regions with the substance immobilized thereon.
摘要翻译：本申请提供了一种制备微珠的方法，包括以下步骤：通过光刻将形成在基材上的薄膜图案化成至少一个预定形状的微珠区域; 将预定物质固定在如此构图的微珠区域上; 并从基材上剥离固定有物质的如此图案化的微珠区域。

5. 发明授权

US08200000B2 Microbead automatic recognition method and microbead 有权
标题翻译：微珠自动识别方法和微珠
公开(公告)号：US08200000B2
公开(公告)日：2012-06-12
申请号：US12432121
申请日：2009-04-29
申请人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
发明人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
IPC分类号： G06K9/00
CPC分类号： G06K19/06037 , G06T7/74 , G06T2207/30072 , G06T2207/30208 , Y10T428/2991
摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.
摘要翻译：微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。

6. 发明申请

US20110136263A1 MICROBEAD ANALYSIS METHOD AND MICROBEAD ANALYZER 失效
标题翻译： MICROBEAD分析方法和MICROBEAD分析仪
公开(公告)号：US20110136263A1
公开(公告)日：2011-06-09
申请号：US12877237
申请日：2010-09-08
申请人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
发明人： Kenzo Machida , Noriyuki Kishii , Mari Ichimura , Kazumine Ito , Takuya Kishimoto , Naohisa Sakamoto
IPC分类号： G01N33/543 , G01N21/64
CPC分类号： G01N33/54366 , G01N21/6452
摘要： Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.
摘要翻译：微珠的微珠分析方法。微珠形成为具有彼此面对的顶表面和底表面的几乎平行的柱状和从其延伸的侧表面，并且携带形成在顶表面和底部中的至少一个上的识别图案表面和固定在其对分析物质具有亲和性的表面上的物质。该方法包括检测由于分析物质与对分析物质具有亲和性的物质的相互作用而从微珠表面发射的荧光，该区域包括不形成识别图案的顶表面和底表面的区域，侧面表面。

7. 发明申请

US20120195507A1 MICROBEAD AUTOMATIC RECOGNITION METHOD AND MICROBEAD 有权
标题翻译： MICROBEAD自动识别方法和MICROBEAD
公开(公告)号：US20120195507A1
公开(公告)日：2012-08-02
申请号：US13440366
申请日：2012-04-05
申请人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
发明人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
IPC分类号： G06K9/46
CPC分类号： G06K19/06037 , G06T7/74 , G06T2207/30072 , G06T2207/30208 , Y10T428/2991
摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.
摘要翻译：微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。

8. 发明申请

US20110183272A1 METHOD FOR FABRICATING MICROBEADS AND MICROBEADS 有权
标题翻译：制备微生物和微生物的方法
公开(公告)号：US20110183272A1
公开(公告)日：2011-07-28
申请号：US13120967
申请日：2009-09-04
申请人： Mari Ichimura , Kenzo Machida , Noriyuki Kishii , Masannobu Tanaka
发明人： Mari Ichimura , Kenzo Machida , Noriyuki Kishii , Masannobu Tanaka
IPC分类号： G03F7/20
CPC分类号： G01N33/54313 , B01J2219/00432 , B01J2219/005 , B01J2219/00502 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00677 , B01J2219/00722
摘要： In one example embodiment, a method fabricates microbeads, which can supply a bead set containing a various types of microbeads and having distinct populations of the respective types of microbeads. In one example embodiment, the method includes forming a hydrophilic layer made of a hydrophilic organic material on a substrate. In one example embodiment, the method includes laminating on the hydrophilic layer a thin film capable of being peeled off in the form of microbeads. In one example embodiment, the method includes forming the thin film in a given configuration by photolithography. In one example embodiment, the method includes solid-phasing a given substance on the post-formed thin films. In one example embodiment, the method includes peeling off the post-formed thin films, which have been solid-phased with the substance, from the substrate along with at least a part of the hydrophilic layer to obtain microbeads.
摘要翻译：在一个示例性实施例中，一种制造微珠的方法，其可以提供包含各种类型的微珠并且具有各种类型的微珠的不同种群的珠粒组。在一个示例性实施例中，该方法包括在基底上形成由亲水性有机材料制成的亲水层。在一个示例性实施例中，该方法包括在亲水层上层叠能够以微珠形式剥离的薄膜。在一个示例性实施例中，该方法包括通过光刻法以给定的配置形成薄膜。在一个示例性实施例中，该方法包括将定影物质固定在后成形薄膜上。在一个示例性实施例中，该方法包括将已经与物质固相化的后成形薄膜与至少一部分亲水层一起从衬底剥离以获得微珠。

9. 发明申请

US20090279787A1 MICROBEAD AUTOMATIC RECOGNITION METHOD AND MICROBEAD 有权
标题翻译： MICROBEAD自动识别方法和MICROBEAD
公开(公告)号：US20090279787A1
公开(公告)日：2009-11-12
申请号：US12432121
申请日：2009-04-29
申请人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
发明人： Noriyuki Kishii , Mari Ichimura , Kenzo Machida
IPC分类号： G06K9/46 , B32B1/00
CPC分类号： G06K19/06037 , G06T7/74 , G06T2207/30072 , G06T2207/30208 , Y10T428/2991
摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.
摘要翻译：微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。

10. 发明授权

US08546068B2 Method for fabricating microbeads and microbeads 有权
标题翻译：微珠和微珠的制备方法
公开(公告)号：US08546068B2
公开(公告)日：2013-10-01
申请号：US13120967
申请日：2009-09-04
申请人： Mari Ichimura , Kenzo Machida , Noriyuki Kishii , Masanobu Tanaka
发明人： Mari Ichimura , Kenzo Machida , Noriyuki Kishii , Masanobu Tanaka
IPC分类号： G03F7/26
CPC分类号： G01N33/54313 , B01J2219/00432 , B01J2219/005 , B01J2219/00502 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00677 , B01J2219/00722
摘要： In one example embodiment, a method fabricates microbeads, which can supply a bead set containing a various types of microbeads and having distinct populations of the respective types of microbeads. In one example embodiment, the method includes forming a hydrophilic layer made of a hydrophilic organic material on a substrate. In one example embodiment, the method includes laminating on the hydrophilic layer a thin film capable of being peeled off in the form of microbeads. In one example embodiment, the method includes forming the thin film in a given configuration by photolithography. In one example embodiment, the method includes solid-phasing a given substance on the post-formed thin films. In one example embodiment, the method includes peeling off the post-formed thin films, which have been solid-phased with the substance, from the substrate along with at least a part of the hydrophilic layer to obtain microbeads.
摘要翻译：在一个示例性实施例中，一种制造微珠的方法，其可以提供包含各种类型的微珠并且具有各种类型的微珠的不同种群的珠粒组。在一个示例性实施例中，该方法包括在基底上形成由亲水性有机材料制成的亲水层。在一个示例性实施例中，该方法包括在亲水层上层叠能够以微珠形式剥离的薄膜。在一个示例性实施例中，该方法包括通过光刻法以给定的配置形成薄膜。在一个示例性实施例中，该方法包括将定影物质固定在后成形薄膜上。在一个示例性实施例中，该方法包括将已经与物质固相化的后成形薄膜与至少一部分亲水层一起从衬底剥离以获得微珠。

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