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    • 5. 发明授权
    • Constitutive expression cassettes for regulation of plant expression
    • 用于调节植物表达的组成型表达盒
    • US08519114B2
    • 2013-08-27
    • US13282973
    • 2011-10-27
    • Ulrich KeetmanUte LinemannKarin HerbersHelke Hillebrand
    • Ulrich KeetmanUte LinemannKarin HerbersHelke Hillebrand
    • C12N15/82C12N15/87C12N15/63
    • C12N15/8216
    • The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable from Arabidopsis thaliana genes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, Atlg64090, At5g60690, Atlg76350, At1g76580, Atlg31930, At5g18230, Atlg20970, or At4g35620. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such an expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector are also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with constitutive transcription regulating activity.
    • 本发明涉及含有从拟南芥基因At5g17920,At3g03780,At2g01100,At2g34770,At5g61560,At4g00830,At3g10220,At4g38520,At3g11110,At2g47170,Atlg64090,At5g60690,Atlg76350,At1g76580中获得的植物中具有组成型表达谱的转录调控序列的表达盒, Atlg31930,At5g18230,Atlg20970或At4g35620。 根据本发明的表达盒可用于表达蛋白质,或表达反义RNA,正义或双链RNA。 优选地,核酸序列的表达赋予植物农学上有价值的性状。 包含这种表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物也被封闭。 还包括用于鉴定和/或分离具有组成型转录调节活性的序列的方法。
    • 6. 发明授权
    • Expression cassettes for root-preferential expression in plants
    • 表达盒在植物中优先表达
    • US07999150B2
    • 2011-08-16
    • US12648651
    • 2009-12-29
    • Ulrich KeetmanUte LinemannKarin HerbersHelke Hillebrand
    • Ulrich KeetmanUte LinemannKarin HerbersHelke Hillebrand
    • C12N15/82C12N15/63A01H5/00
    • C12N15/8227C07K14/415
    • The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.
    • 本发明涉及包含从拟南芥基因At1g66280,At1g74500,At1g49570,At1g70710,At5g66690,At3g29630,At5g48070或At4g17800获得的植物中具有根优先或根特异性表达谱的转录调节序列的表达盒。 根据本发明的表达盒可用于表达蛋白质,或表达反义RNA,正义或双链RNA。 优选地,核酸序列的表达赋予植物农学上有价值的性状。 包含该表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物体也被封闭。 还包括用于鉴定和/或分离具有根特异性或根优先转录调节活性的序列的方法。
    • 7. 发明授权
    • Expression cassettes for root-preferential expression in plants
    • 表达盒在植物中优先表达
    • US07667098B2
    • 2010-02-23
    • US12041253
    • 2008-03-03
    • Ulrich KeetmanUte LinemannKarin HerbersHelke Hillebrand
    • Ulrich KeetmanUte LinemannKarin HerbersHelke Hillebrand
    • A01H1/00C12N15/82C12N15/87C07H21/04C12N15/00
    • C12N15/8227C07K14/415
    • The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.
    • 本发明涉及包含从拟南芥基因At1g66280,At1g74500,At1g49570,At1g70710,At5g66690,At3g29630,At5g48070或At4g17800获得的植物中具有根优先或根特异性表达谱的转录调节序列的表达盒。 根据本发明的表达盒可用于表达蛋白质,或表达反义RNA,正义或双链RNA。 优选地,核酸序列的表达赋予植物农学上有价值的性状。 包含该表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物体也被封闭。 还包括用于鉴定和/或分离具有根特异性或根优先转录调节活性的序列的方法。