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1. 发明授权

US07345216B2 Expression cassettes for meristem-preferential expression in plants 有权
标题翻译：用于分生组织优先表达在植物中的表达盒
公开(公告)号：US07345216B2
公开(公告)日：2008-03-18
申请号：US11297315
申请日：2005-12-08
申请人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
发明人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
IPC分类号： C12N15/82 , A01H5/10 , C07H21/04
CPC分类号： C12N15/8229 , C07K14/415 , C12N15/8226 , C12N15/8227
摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with meristem-preferential or meristem-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At2g02180, At5g54510, At2g26970, At2g01180, At3g45560, At4g00580, At1g54480, or At4g11490, or the Arabidopsis thaliana genomic sequences as described by SEQ ID NO: 35 or 36.
摘要翻译：本发明涉及包含从拟南芥基因At2g02180，At5g54510，At2g26970，At2g01180，At3g45560，At4g00580，At1g54480或At4g11490或拟南芥基因组获得的植物中具有分生组织优先或分生组织特异性表达谱的转录调节序列的表达盒 SEQ ID NO：35或36所述的序列。

2. 发明授权

US07335813B2 Expression cassettes for guard cell-preferential expression in plants 有权
标题翻译：用于在植物中保护细胞优先表达的表达盒
公开(公告)号：US07335813B2
公开(公告)日：2008-02-26
申请号：US11285674
申请日：2005-11-22
申请人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
发明人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
IPC分类号： C12N15/82 , A01H5/10 , C07H21/04
CPC分类号： C12N15/8225
摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with guard cell-preferential or guard cell-specific expression profiles in plants obtainable from Arabidopsis thaliana gene At5g58580, or from Arabidopsis genomic DNA sequences as described by SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 13, 14, or 15.
摘要翻译：本发明涉及包含从拟南芥基因At5g58580获得的植物中的保护性细胞优先或保卫细胞特异性表达谱的转录调控序列的表达盒，或如SEQ ID NO：1,2,3所描述的拟南芥基因组DNA序列，4，5，6，7，8，9，12，13，14或15。

3. 发明申请

US20060117415A1 Expression cassettes for root-preferential expression in plants 失效
标题翻译：表达盒在植物中优先表达
公开(公告)号：US20060117415A1
公开(公告)日：2006-06-01
申请号：US11285701
申请日：2005-11-22
申请人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
发明人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8227 , C07K14/415
摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800.
摘要翻译：本发明涉及包含从拟南芥基因At1g66280，At1g74500，At1g49570，At1g70710，At5g66690，At3g29630，At5g48070或At4g17800获得的植物中具有根优先或根特异性表达谱的转录调节序列的表达盒。

4. 发明授权

US07557203B2 Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效
标题翻译：用于植物中核酸双向转录表达的表达盒
公开(公告)号：US07557203B2
公开(公告)日：2009-07-07
申请号：US10565221
申请日：2004-07-03
申请人： Ute Linemann , Karin Herbers , Irene Kunze
发明人： Ute Linemann , Karin Herbers , Irene Kunze
IPC分类号： C12N15/82 , C12N15/00 , A01H5/00 , A01H5/10 , C12N15/11 , C12N15/63
CPC分类号： C12N15/8216
摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.
摘要翻译：本发明涉及用于在植物细胞中表达两种核酸序列的转基因表达盒，其包含至少一种选自a）SEQ ID NO：1或2所示的启动子的调节序列，b）启动子的功能等同物如SEQ ID NO：1或2所示，其与SEQ ID NO：1或2所示的序列具有至少80％的同一性，并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性，c）SEQ ID NO：1或2所示的启动子的功能等同物，其包含SEQ ID NO：1或2所示序列的至少25个连续核苷酸并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性 SEQ ID NO：1或2，以及d）序列a）或b）或c）的功能上等同的片段，其具有所述序列a）或b）或c）的至少25个连续核苷酸，并且具有基本上相同的启动子活性 SEQ ID NO：1或2所示的启动子，其中所述调节元件设置在两个核酸序列之间并且与所述核酸序列相关并且与所述核酸序列功能性连接，使得两个不同的核糖核酸序列的表达在至少一个植物细胞，其中所述核糖核酸序列选自编码i）氨基酸序列的核糖核酸序列或ii）导致所述植物细胞的至少一个内源基因表达降低的核糖核酸序列。

5. 发明申请

US20060150282A1 Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效
公开(公告)号：US20060150282A1
公开(公告)日：2006-07-06
申请号：US10565221
申请日：2004-07-03
申请人： Ute Linemann , Karin Herbers , Irene Kunze
发明人： Ute Linemann , Karin Herbers , Irene Kunze
IPC分类号： C12N5/04 , C12N15/82 , A01H1/00
CPC分类号： C12N15/8216
摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.

6. 发明申请

US20060117408A1 Expression cassettes for guard cell-preferential expression in plants 有权
标题翻译：用于在植物中保护细胞优先表达的表达盒
公开(公告)号：US20060117408A1
公开(公告)日：2006-06-01
申请号：US11285674
申请日：2005-11-22
申请人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
发明人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
IPC分类号： A01H1/00 , C12Q1/68 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8225
摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with guard cell-preferential or guard cell-specific expression profiles in plants obtainable from Arabidopsis thaliana gene At5g58580, or from Arabidopsis genomic DNA sequences as described by SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 13, 14, or 15.
摘要翻译：本发明涉及包含从拟南芥基因At5g58580获得的植物中的保护性细胞优先或保卫细胞特异性表达谱的转录调控序列的表达盒，或如SEQ ID NO：1,2,3所描述的拟南芥基因组DNA序列，4，5，6，7，8，9，12，13，14或15。

7. 发明授权

US08519114B2 Constitutive expression cassettes for regulation of plant expression 失效
标题翻译：用于调节植物表达的组成型表达盒
公开(公告)号：US08519114B2
公开(公告)日：2013-08-27
申请号：US13282973
申请日：2011-10-27
申请人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
发明人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
IPC分类号： C12N15/82 , C12N15/87 , C12N15/63
CPC分类号： C12N15/8216
摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable from Arabidopsis thaliana genes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, Atlg64090, At5g60690, Atlg76350, At1g76580, Atlg31930, At5g18230, Atlg20970, or At4g35620. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such an expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector are also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with constitutive transcription regulating activity.
摘要翻译：本发明涉及含有从拟南芥基因At5g17920，At3g03780，At2g01100，At2g34770，At5g61560，At4g00830，At3g10220，At4g38520，At3g11110，At2g47170，Atlg64090，At5g60690，Atlg76350，At1g76580中获得的植物中具有组成型表达谱的转录调控序列的表达盒， Atlg31930，At5g18230，Atlg20970或At4g35620。根据本发明的表达盒可用于表达蛋白质，或表达反义RNA，正义或双链RNA。优选地，核酸序列的表达赋予植物农学上有价值的性状。包含这种表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物也被封闭。还包括用于鉴定和/或分离具有组成型转录调节活性的序列的方法。

8. 发明授权

US07999150B2 Expression cassettes for root-preferential expression in plants 失效
标题翻译：表达盒在植物中优先表达
公开(公告)号：US07999150B2
公开(公告)日：2011-08-16
申请号：US12648651
申请日：2009-12-29
申请人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
发明人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
IPC分类号： C12N15/82 , C12N15/63 , A01H5/00
CPC分类号： C12N15/8227 , C07K14/415
摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.
摘要翻译：本发明涉及包含从拟南芥基因At1g66280，At1g74500，At1g49570，At1g70710，At5g66690，At3g29630，At5g48070或At4g17800获得的植物中具有根优先或根特异性表达谱的转录调节序列的表达盒。根据本发明的表达盒可用于表达蛋白质，或表达反义RNA，正义或双链RNA。优选地，核酸序列的表达赋予植物农学上有价值的性状。包含该表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物体也被封闭。还包括用于鉴定和/或分离具有根特异性或根优先转录调节活性的序列的方法。

9. 发明授权

US07667098B2 Expression cassettes for root-preferential expression in plants 失效
标题翻译：表达盒在植物中优先表达
公开(公告)号：US07667098B2
公开(公告)日：2010-02-23
申请号：US12041253
申请日：2008-03-03
申请人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
发明人： Ulrich Keetman , Ute Linemann , Karin Herbers , Helke Hillebrand
IPC分类号： A01H1/00 , C12N15/82 , C12N15/87 , C07H21/04 , C12N15/00
CPC分类号： C12N15/8227 , C07K14/415
摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.
摘要翻译：本发明涉及包含从拟南芥基因At1g66280，At1g74500，At1g49570，At1g70710，At5g66690，At3g29630，At5g48070或At4g17800获得的植物中具有根优先或根特异性表达谱的转录调节序列的表达盒。根据本发明的表达盒可用于表达蛋白质，或表达反义RNA，正义或双链RNA。优选地，核酸序列的表达赋予植物农学上有价值的性状。包含该表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物体也被封闭。还包括用于鉴定和/或分离具有根特异性或根优先转录调节活性的序列的方法。

10. 发明申请

US20120117676A1 Soybean Event 127 and Methods Related Thereto 有权
标题翻译：大豆事件127及相关方法
公开(公告)号：US20120117676A1
公开(公告)日：2012-05-10
申请号：US13143491
申请日：2010-01-06
申请人： Dale Carlson , Francisco Jose Lima Aragao , Carlos Alberto Arrabal Arias , Luiz Louzano , Bruce M. Luzzi , Tim Malefyt , Elibio Leopoldo Rech Filho , Siyuan Tan , Adolfo Ulbrich , Tadashi Yotsumoto , Ute Linemann
发明人： Dale Carlson , Francisco Jose Lima Aragao , Carlos Alberto Arrabal Arias , Luiz Louzano , Bruce M. Luzzi , Tim Malefyt , Elibio Leopoldo Rech Filho , Siyuan Tan , Adolfo Ulbrich , Tadashi Yotsumoto , Ute Linemann
IPC分类号： A01N43/54 , A01H5/00 , C07H21/04 , C12Q1/68 , A01N61/00 , A01H5/10 , G01N33/566 , C07K17/00 , C40B40/06 , C40B40/10 , C12N15/11 , A01H1/02
CPC分类号： A01H5/10 , A01H1/04 , C12N15/8274 , C12N15/8275 , C12Q1/6895
摘要： Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location may comprise genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.
摘要翻译：提供了与转基因AHAS抑制除草剂抗性大豆植物相关的组合物和方法。提供了具有突变的AHAS编码序列的127种大豆植物，其赋予对AHAS抑制性除草剂的耐受性。在确定的染色体位置处具有事件127核酸分子的127个大豆植物的事件可以包含至少具有SEQ ID NO：5和/或6的核酸序列的基因组/转基因接头。事件127提供增强的育种效率，并且能够使用分子标记来跟踪育种种群中的转基因插入物及其后代。提供用于鉴定，检测和使用事件127种大豆植物的各种方法和组合物。

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