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1. 发明授权

US6013860A Expression of enzymes involved in cellulose modification 失效
标题翻译：参与纤维素修饰的酶的表达
公开(公告)号：US6013860A
公开(公告)日：2000-01-11
申请号：US122533
申请日：1998-07-24
申请人： Michael E. Himmel , David J. Schaaf , David M. Stalker , Steven R. Thomas
发明人： Michael E. Himmel , David J. Schaaf , David M. Stalker , Steven R. Thomas
IPC分类号： A01H5/00 , C12N5/10 , C12N9/24 , C12N15/09 , C12N15/53 , C12N15/82 , C07H21/02 , C07H21/04 , C12N5/04
CPC分类号： C12N15/8246 , C12N15/8214
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide expression in the plastids of a plant or plant cell of cellulose degrading enzymes.
摘要翻译：用于植物细胞遗传工程的新型组合物和方法，用于在纤维素降解酶的植物或植物细胞的质体中提供表达。

2. 发明授权

US06541682B1 Plastid transformation of solanaceous plants 有权
公开(公告)号：US06541682B1
公开(公告)日：2003-04-01
申请号：US09191303
申请日：1998-11-12
申请人： Narender S. Nehra , David J. Schaaf , Vladimir Sidorov , David M. Stalker , Guangning Ye
发明人： Narender S. Nehra , David J. Schaaf , Vladimir Sidorov , David M. Stalker , Guangning Ye
IPC分类号： C12N1582
CPC分类号： C12N15/8216 , C12N15/8214
摘要： A method is provided for transforming solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5′ to 3′ direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilized in the transformation of solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid.

3. 发明授权

US06492578B1 Expression of herbicide tolerance genes in plant plastids 失效
标题翻译：除草剂耐受基因在植物质体中的表达
公开(公告)号：US06492578B1
公开(公告)日：2002-12-10
申请号：US09351123
申请日：1999-07-10
申请人： Jeffrey M. Staub , Peter Hajdukiewicz , Kevin E. McBride , Narender Nehra , David J. Schaaf , David M. Stalker , Guangning Ye
发明人： Jeffrey M. Staub , Peter Hajdukiewicz , Kevin E. McBride , Narender Nehra , David J. Schaaf , David M. Stalker , Guangning Ye
IPC分类号： C12N1582
CPC分类号： C12N9/1092 , C07K2319/00 , C12N9/14 , C12N9/88 , C12N15/8214 , C12N15/8274 , C12N15/8275 , C12N15/8278
摘要： Provided are constructs and methods for expressing herbicide tolerance genes in plastids of plant cells. Constructs include the components of a promoter functional in a plant plastid, a DNA sequence which is capable of conferring tolerance in a plant cell to at least one herbicide compound when said DNA sequence is transcribed in plastids of said plant cell and a transcription termination region. Herbicide tolerance is produced by transforming plastids with the constructs of the invention and growing plant cells comprising the transformed plastids under conditions wherein the DNA sequence is transcribed and plant plastids and cells containing the plastids are rendered tolerant to applications of at least one herbicide compound.
摘要翻译：提供了用于在植物细胞的质体中表达除草剂耐受基因的构建体和方法。构建体包括在植物质体中起作用的启动子的组分，当所述DNA序列在所述植物细胞的质体和转录终止区中转录时，能够赋予植物细胞对至少一种除草剂化合物的耐受性的DNA序列。通过用本发明的构建体转化质体和包含转化的质体的条件下生长的植物细胞产生除草剂耐受性，其中DNA序列被转录并且植物质体和包含质体的细胞对至少一种除草剂化合物的施用具有耐受性。

4. 发明授权

US06512162B2 Expression of eukaryotic peptides in plant plastids 失效
标题翻译：真核肽在植物质体中的表达
公开(公告)号：US06512162B2
公开(公告)日：2003-01-28
申请号：US09113244
申请日：1998-07-10
申请人： Kevin E. McBride , Narender Nehra , Douglas A. Russell , David M. Stalker , Jeffrey M. Staub
发明人： Kevin E. McBride , Narender Nehra , Douglas A. Russell , David M. Stalker , Jeffrey M. Staub
IPC分类号： C12N1582
CPC分类号： C12N15/8275 , C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences re expressed to produce eukaryotic peptide in said plastid.
摘要翻译：提供构建体和方法用于表达在植物质体中衍生自真核生物的肽。构建体在植物质体中具有功能的启动子，编码来自真核生物的肽的DNA序列和转录终止区。其他元件包括用于选择植物细胞的选择性标记，其包括表达标记的质体和与质体的基因组同源的DNA区以及任选的与启动子连接的核糖体结合位点。通过使用这种构建的方法，通过在条件下生长植物细胞，在DNA质粒编码序列中再次表达以在所述质体中产生真核肽，从而在植物细胞中产生高水平的真核肽，例如哺乳动物蛋白质。

5. 发明授权

US5576198A Controlled expression of transgenic constructs in plant plastids 失效
标题翻译：植物质体中转基因构建体的受控表达
公开(公告)号：US5576198A
公开(公告)日：1996-11-19
申请号：US167638
申请日：1993-12-14
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/82 , A01H4/00
CPC分类号： C12N15/8214
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide a method of controlling the timing or tissue pattern of expression of foreign DNA sequences inserted into the plant plastid genome are provided in the instant invention. Constructs include those for nuclear transformation which provide for expression of a viral single subunit RNA polymerase in plant cell tissues, and targeting of the expressed polymerase protein into the plant cell plastids. In addition, plastid expression constructs comprising a viral gene promoter region which is specific to the RNA polymerase expressed from the nuclear expression constructs described above, and a DNA sequence of interest to be expressed in the transformed plastid cells are provided. Plant cells and plants comprising the nuclear and/or plastid constructs described herein are of interest. Of particular interest is a method of controlling expression of the inserted plastid gene constructs in a tissue and/or developmental specific manner in plants comprising both the nuclear polymerase construct and the plastid expression constructs.
摘要翻译：在本发明中提供了可用于植物细胞遗传工程的新型组合物和方法，以提供控制插入植物质体基因组中的外来DNA序列的表达的时间或组织模式的方法。构建体包括提供用于在植物细胞组织中表达病毒单亚基RNA聚合酶的核转化的构建体，以及将表达的聚合酶蛋白靶向植物细胞质体。此外，提供了包含由上述核表达构建体表达的RNA聚合酶特异的病毒基因启动子区和在转化的质体细胞中表达的目标DNA序列的质体表达构建体。包含本文所述的核和/或质体构建体的植物细胞和植物是令人感兴趣的。特别感兴趣的是在包含核聚合酶构建体和质体表达构建体的植物中以组织和/或发育特异性方式控制插入的质体基因构建体的表达的方法。

6. 发明授权

US4810648A Haloarylnitrile degrading gene, its use, and cells containing the gene 失效
标题翻译：卤代芳基腈降解基因，其用途和含有该基因的细胞
公开(公告)号：US4810648A
公开(公告)日：1989-03-07
申请号：US71146
申请日：1987-07-08
申请人： David M. Stalker
发明人： David M. Stalker
IPC分类号： C12N5/02 , C12N9/78 , C12N15/55 , C12N15/63 , C12N15/64 , C12N15/70 , C12N15/82 , C12N9/06 , A01H1/00 , C12N5/00
CPC分类号： C12N15/63 , C12N15/64 , C12N15/70 , C12N15/8274 , C12N9/78
摘要： Nitrilase enzymes specific for the hyrdolsis of the nitrile group of bromoxynil, nucleotide sequences encoding for such enzymes, and transformed cells in which the nitrilase expression if foreign are provided. The transformed cells are capable of expressing the nitrilase enzyme to provide detoxification of an environment and protect bromoxynil-sensitive cells from its cytotoxic effect. Particularly, plants are developed which are resistant to bromoxynil.E. coli MM294 strain (pBrx5) was deposited at the A.T.C.C. on Jan. 22, 1986 and given Accession no. 53435.E. coli MM294 strain (pBrx11) was deposited at the A.T.C.C. on June 18, 1987 and given Accession no. 67441.E. coli MM294 strain (pBrx23) was deposited at the A.T.C.C. on June 18, 1987 and given Accession no. 67442.
摘要翻译：溴苯腈的腈基特异性的腈水解酶，编码这些酶的核苷酸序列以及其中提供外源的腈水解酶表达的转化细胞。转化细胞能够表达腈水解酶以提供环境的解毒，并保护溴苯腈敏感细胞免受其细胞毒性作用。特别地，开发出抗溴苯腈耐受性的植物。大肠杆菌MM294菌株（pBrx5）保藏在A.T.C.C. 1986年1月22日，加拿大号大肠杆菌MM294菌株（pBrx11）保藏在A.T.C.C. 1987年6月18日，加拿大号大肠杆菌MM294菌株（pBrx23）保藏在A.T.C.C. 1987年6月18日，加拿大号 67442。

7. 发明授权

US07732678B1 Cotton fiber transcriptional factors 失效
标题翻译：棉纤维转录因子
公开(公告)号：US07732678B1
公开(公告)日：2010-06-08
申请号：US08984099
申请日：1997-12-03
申请人： Kevin McBride , David M. Stalker , Julie R. Pear , Luis Perez-Grau
发明人： Kevin McBride , David M. Stalker , Julie R. Pear , Luis Perez-Grau
IPC分类号： A01H5/00 , C07H21/04 , C12N15/82
CPC分类号： C12N9/0071 , C07K14/36 , C07K14/415 , C12N9/0069 , C12N9/88 , C12N15/8222 , C12N15/8233 , C12N15/825 , C12N15/8261 , Y02A40/146
摘要： Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest in cotton fiber, particularly in very early fiber development. The DNA constructs comprise a cotton fiber transcriptional initiation regulatory region associated with a gene which is expressed in cotton fiber.
摘要翻译：提供了新的DNA构建体，其可以用作分子探针或插入到植物宿主中，以提供对棉花纤维中感兴趣的DNA序列的转录的修饰，特别是在非常早期的纤维发育中。 DNA构建体包含与在棉纤维中表达的基因相关的棉花纤维转录起始调节区。

8. 发明授权

US06576818B1 Plant cellulose synthase and promoter sequences 有权
公开(公告)号：US06576818B1
公开(公告)日：2003-06-10
申请号：US09838586
申请日：2001-04-18
申请人： David M. Stalker , Julie R. Pear , Deborah Delmer
发明人： David M. Stalker , Julie R. Pear , Deborah Delmer
IPC分类号： A01H500
CPC分类号： C12N9/1059 , C12N15/8222 , C12N15/8233 , C12N15/8242 , C12N15/8246 , C12N15/8261 , Y02A40/146
摘要： Provided are two plant cDNA clones that are homologs of the bacterial CelA genes that encode the catalytic subunit of cellulose synthase, derived from cotton (Gossypium hirsutum). Also provided are genomic promoter regions to these encoding regions to cellulose synthase. Methods for using cellulose synthase in cotton fiber and wood quality modification are also provided.

9. 发明授权

US06222097B1 Use of ovary-tissue transcriptional factors for altering plant color 失效
标题翻译：使用卵巢组织转录因子改变植物的颜色
公开(公告)号：US06222097B1
公开(公告)日：2001-04-24
申请号：US08984320
申请日：1997-12-03
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
IPC分类号： A01H100
CPC分类号： C07K14/415 , C07K2319/00 , C07K2319/02 , C12N9/0071 , C12N15/8218 , C12N15/8222 , C12N15/8233 , C12N15/825
摘要： Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest in ovary tissue, particularly in very early fruit development. The DNA constructs comprise a transcriptional initiation regulatory region associated with gene expression in ovary tissue from immediately prior to anthesis through flower senescence.
摘要翻译：提供了新的DNA构建体，其可以用作分子探针或插入到植物宿主中以提供对于在卵巢组织中感兴趣的DNA序列的转录的修饰，特别是在非常早期的果实发育中。 DNA构建体包含与通过花衰老开花之前的卵巢组织中与基因表达相关的转录起始调节区。

10. 发明授权

US5866421A Enhanced expression in a plant plastid 失效
标题翻译：在植物质体中增强表达
公开(公告)号：US5866421A
公开(公告)日：1999-02-02
申请号：US593205
申请日：1996-01-29
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
IPC分类号： A01H5/00 , C07K14/325 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/02 , C12N5/14
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/8214 , C12N15/8216 , C12N15/8223
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.
摘要翻译：用于植物细胞遗传工程的新型组合物和方法可用于提供蛋白质的植物或植物细胞的质体中增加的表达，该蛋白质产生当在不存在选择转化的方法的情况下生长植物或植物细胞时存在的表型细胞。苏云金芽孢杆菌细菌原毒素在植物叶绿体中的表达。

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