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1. 发明授权

US08759043B2 Biocatalytic production of ambroxan 有权
标题翻译：生物催化生产氨溴索
公开(公告)号：US08759043B2
公开(公告)日：2014-06-24
申请号：US13375996
申请日：2010-06-02
申请人： Michael Breuer , Andrea Hörster , Bernhard Hauer
发明人： Michael Breuer , Andrea Hörster , Bernhard Hauer
IPC分类号： C12P17/04 , C12N1/15 , C12N1/19 , C12N1/21
CPC分类号： C12P17/04 , C12N9/90
摘要： The invention relates to a process for the biocatalytic production of ambroxan by means of a polypeptide with the activity of a homofarnesol-ambroxan cyclase, which are a novel class of enzymes.
摘要翻译：本发明涉及一种通过具有活性的同型异恶唑烷醇 - 氨溴环己烷酶的多肽生物催化生产氨溴辛的方法，该酶是新一类酶。

2. 发明申请

US20120135477A1 Biocatalytic Production of Ambroxan 有权
标题翻译： Ambroxan的生物催化生产
公开(公告)号：US20120135477A1
公开(公告)日：2012-05-31
申请号：US13375996
申请日：2010-06-02
申请人： Michael Breuer , Andrea Hörster , Bernhard Hauer
发明人： Michael Breuer , Andrea Hörster , Bernhard Hauer
IPC分类号： C12P17/04 , C12N1/15 , C12N1/19 , C12N15/63 , C12N1/21
CPC分类号： C12P17/04 , C12N9/90
摘要： The invention relates to a process for the biocatalytic production of ambroxan by means of a polypeptide with the activity of a homofarnesol-ambroxan cyclase, which are a novel class of enzymes.
摘要翻译：本发明涉及一种通过具有活性的同型异恶唑烷醇 - 氨溴环己烷酶的多肽生物催化生产氨溴辛的方法，该酶是新一类酶。

3. 发明授权

US08932839B2 Method for the biocatalytic cyclization of terpenes and cyclase mutants employable therein 有权
标题翻译：用于其中可用的萜烯和环化酶突变体的生物催化环化的方法
公开(公告)号：US08932839B2
公开(公告)日：2015-01-13
申请号：US13297798
申请日：2011-11-16
申请人： Michael Breuer , Bernhard Hauer , Dieter Jendrossek , Gabriele Siedenburg , Juergen Pleiss , Demet Sirim , Silvia Racolta
发明人： Michael Breuer , Bernhard Hauer , Dieter Jendrossek , Gabriele Siedenburg , Juergen Pleiss , Demet Sirim , Silvia Racolta
IPC分类号： C12P5/00 , C12N1/19 , C12P7/02 , C12N9/88
CPC分类号： C12N9/88 , C12N9/90 , C12P5/007 , C12P7/02 , C12P7/22 , C12Y406/01 , C12Y504/99017 , Y02P20/52
摘要： The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
摘要翻译：本发明涉及具有环化酶活性的新颖突变体及其在萜烯生物催化环化方法中的应用，特别是通过香茅醛环化生产异胡胆糖; 用于制备薄荷醇的方法和用于生物催化转化具有类似于萜烯的结构基序的其它化合物的方法。

4. 发明授权

US08617854B2 Method for producing L-phenylephrine using an alcohol dehydrogenase of Aromatoleum aromaticum EBN1 (Azoarcus sp. EBN1) 有权
标题翻译：使用芳香香草EBN1（Azoarcus sp。EBN1）的醇脱氢酶生产L-苯肾上腺素的方法
公开(公告)号：US08617854B2
公开(公告)日：2013-12-31
申请号：US13063630
申请日：2009-09-15
申请人： Michael Breuer , Andreas Pletsch , Bernhard Hauer , Wolfgang Siegel
发明人： Michael Breuer , Andreas Pletsch , Bernhard Hauer , Wolfgang Siegel
IPC分类号： C12P7/22 , C12P13/00
CPC分类号： C12P13/001 , C12P7/22
摘要： The present invention relates to a multi-stage process for producing substituted, optically active alcohols, comprising an enzyme-catalyzed synthesis step, in particular a synthesis step which is catalyzed by an alcohol dehydrogenase. The inventive method is particularly suitable for producing phenylephrine, i.e. 3-[(1R)-1-hydroxy-2-methylamino-ethyl]-phenol.
摘要翻译：本发明涉及一种制备取代的光学活性醇的多阶段方法，其包括酶催化的合成步骤，特别是由醇脱氢酶催化的合成步骤。本发明的方法特别适用于生产去氧肾上腺素，即3 - [（1R）-1-羟基-2-甲基氨基 - 乙基] - 苯酚。

5. 发明授权

US08012725B2 Method for the production of optically-active alcohols 有权
标题翻译：光学活性醇的制造方法
公开(公告)号：US08012725B2
公开(公告)日：2011-09-06
申请号：US11667721
申请日：2005-11-15
申请人： Rainer Stürmer , Bernhard Hauer , Dejana Drew , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Bernhard Hauer , Dejana Drew , Michael Breuer , Hartwig Schröder
IPC分类号： C12P7/16 , C12P7/02 , C12P7/04 , C12Q1/26 , C12Q1/32 , C12N1/00 , C12N1/20 , C12N9/02 , C07H21/04
CPC分类号： C12P7/16 , Y02E50/10
摘要： The present invention relates to enzymic processes for preparing S-butan-2-ol; and to enzymes for carrying out said processes; to nucleic acid sequences coding for said enzymes, to expression cassettes, vectors and recombinant hosts containing said nucleic acid sequences.
摘要翻译：本发明涉及制备S-丁-2-醇的酶法; 和用于进行所述方法的酶; 编码所述酶的核酸序列，含有所述核酸序列的表达盒，载体和重组宿主。

6. 发明申请

US20100068760A1 PROTEINS WITH ESTERASE ACTIVITY 有权
标题翻译：蛋白质与蛋白酶活性
公开(公告)号：US20100068760A1
公开(公告)日：2010-03-18
申请号：US12306703
申请日：2007-06-26
申请人： Michael Breuer , Bernhard Hauer , Ulrich Schwaneberg , Tuck Seng Wong
发明人： Michael Breuer , Bernhard Hauer , Ulrich Schwaneberg , Tuck Seng Wong
IPC分类号： C12P21/00 , C12N9/16 , C07H21/00 , C12N15/63 , C12N1/00 , C12P7/62
CPC分类号： C12N9/16 , C12P7/62 , C12P41/003
摘要： The invention relates to novel proteins having esterase activity, to mutants thereof, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.
摘要翻译：本发明涉及具有酯酶活性的新型蛋白质，其突变体与其编码的核酸序列，表达盒，载体和重组微生物; 涉及用于制备所述蛋白质的方法及其用于酶，特别是对映选择性酶，酯水解或酯交换有机酯的用途。

7. 发明申请

US20090104657A1 Lipase Production Method 审中-公开
标题翻译：脂肪酶生产方法
公开(公告)号：US20090104657A1
公开(公告)日：2009-04-23
申请号：US11922176
申请日：2006-06-13
申请人： Anke Beselin , Frank Rosenau , Karl-Erich Jaeger , Michael Breuer , Bernhard Hauer
发明人： Anke Beselin , Frank Rosenau , Karl-Erich Jaeger , Michael Breuer , Bernhard Hauer
IPC分类号： C12P21/00 , C07K14/00 , C12N9/48 , C07H21/00 , C12N15/63 , C12N1/21
CPC分类号： C12N9/52 , C12N9/20
摘要： The invention relates to novel proteins, especially with protease activity, which promote the production of extracellular lipases by bacteria, especially of the genus Burkholderia, nucleic acid sequences encoding them, expression constructs containing them, hosts transformed with them, methods for the production of the proteins with protease activity and methods for the production of lipases, especially by bacteria of the genus Burkholderia.
摘要翻译：本发明涉及特别是蛋白酶活性的新型蛋白质，其促进细菌特别是伯克霍尔德杆菌属细菌细胞外脂肪酶的生产，编码它们的核酸序列，含有它们的表达构建体，用它们转化的宿主，用于生产具有蛋白酶活性的蛋白质和生产脂肪酶的方法，特别是伯克霍尔德氏菌属的细菌。

8. 发明申请

US20080318288A1 Enzymatic Reduction for Producing Optically Active Alcohols 有权
标题翻译：用于生产光活性醇的酶减量
公开(公告)号：US20080318288A1
公开(公告)日：2008-12-25
申请号：US12158390
申请日：2006-12-13
申请人： Rainer Stürmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
IPC分类号： C12P17/00 , C12N9/14
CPC分类号： C07D333/16 , C07D333/20 , C12P7/02 , C12P11/00 , C12P41/002
摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.
摘要翻译：一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

9. 发明授权

US09187737B2 Mutants of a pseudomonas glumae butynol esterase 有权
标题翻译：假单胞菌乳酸丁酯酯酶的突变体
公开(公告)号：US09187737B2
公开(公告)日：2015-11-17
申请号：US12306703
申请日：2007-06-26
申请人： Michael Breuer , Bernhard Hauer , Ulrich Schwaneberg , Tuck Seng Wong
发明人： Michael Breuer , Bernhard Hauer , Ulrich Schwaneberg , Tuck Seng Wong
IPC分类号： C12P7/62 , C12N9/18 , C12N9/16 , C12P41/00
CPC分类号： C12N9/16 , C12P7/62 , C12P41/003
摘要： The invention relates to novel proteins having esterase activity, to mutants thereof, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.
摘要翻译：本发明涉及具有酯酶活性的新型蛋白质，其突变体与其编码的核酸序列，表达盒，载体和重组微生物; 涉及用于制备所述蛋白质的方法及其用于酶，特别是对映选择性酶，酯水解或酯交换有机酯的用途。

10. 发明申请

US20120285816A1 METHOD FOR ISOLATING AN ALKANOL FROM AN AQUEOUS BIOTRANSFORMATION MIXTURE 审中-公开
标题翻译：从水溶性生物混合物中分离出烷基醇的方法
公开(公告)号：US20120285816A1
公开(公告)日：2012-11-15
申请号：US13511594
申请日：2010-11-24
申请人： Jürgen Däuwel , Michael Breuer , Bernhard Hauer
发明人： Jürgen Däuwel , Michael Breuer , Bernhard Hauer
IPC分类号： B01D3/36
CPC分类号： C07C29/82 , C07B2200/07 , C07C29/80 , C07C29/86 , C07C31/12 , C07C31/125
摘要： The invention relates to the isolation of an alkanol from an aqueous biotransformation mixture, in that a) a first alkanol phase is obtained by means of distilling out an alkanol-water azeotrope from the aqueous biotransformation mixture and, if the azeotrope is a heteroazeotrope, phase separating the azeotrope and separating out an aqueous phase, b) a second alkanol phase is obtained by (i) liquid/liquid extracting the first alkanol phase using a solvent as an extracting agent, or (ii) azeotropic drying the first alkanol phase in the presence of the solvent as a carrier agent, and c) the second alkanol phase is fractionally distilled, producing a pure alkanol fraction. The biotransformation mixture is obtained, for example, by means of reducing an alkanol in the presence of an alcohol dehydrogenase. The method is adapted to the severe dilution of the products of value in the biotransformation mixture and works without long phase separation times when extracting by means of organic solvents.
摘要翻译：本发明涉及从水生物转化混合物中分离链烷醇，其中a）通过从水生物转化混合物中蒸馏出链烷醇 - 水共沸物获得第一链烷醇相，如果共沸物是杂合物，则相分离共沸物并分离水相，b）通过以下步骤获得第二链烷醇相：（i）使用溶剂作为萃取剂液/液萃取第一链烷醇相，或（ii）共沸干燥第一链烷醇相作为载体剂的溶剂的存在，和c）分馏第二链烷醇相，产生纯的链烷醇级分。生物转化混合物例如通过在醇脱氢酶的存在下还原链烷醇获得。该方法适用于在生物转化混合物中严重稀释有价值的产物，并在通过有机溶剂萃取时无长时间分离时间。

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