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1. 发明申请

US20080301823A1 Polynucleotide for Producing Recombinant Protein in Silkworm 审中-公开
标题翻译：用于在蚕中产生重组蛋白的多核苷酸
公开(公告)号：US20080301823A1
公开(公告)日：2008-12-04
申请号：US11665396
申请日：2005-10-14
申请人： Masahiro Tomita , Katsuhiko Shimizu , Shingo Ogawa , Rika Hino , Masashi Iizuka , Takahiro Adachi , Katsutoshi Yoshizato
发明人： Masahiro Tomita , Katsuhiko Shimizu , Shingo Ogawa , Rika Hino , Masashi Iizuka , Takahiro Adachi , Katsutoshi Yoshizato
IPC分类号： A01K67/027 , C12N15/11 , C12N15/00
CPC分类号： C12P21/02 , A01K67/0339 , A01K67/04 , A01K2217/05 , A01K2227/706 , A01K2267/01 , C07K14/43586
摘要： As novel means for causing any recombinant protein produced by the silkworm middle silk gland to be secreted in cocoons and easily extracting the recombinant protein from the cocoons without modification of the conformation of the protein, there is provided a polynucleotide that in an expression cassette for expression of a recombinant protein by the silkworm middle silk gland, is functionally linked to a recombinant protein structure gene, which polynucleotide is one composed of a polynucleotide constructing the promoter regions of sericin 1 or sericin 2 gene and, functionally linked thereto, a polynucleotide constructing the homologous regions of baculovirus.
摘要翻译：作为使蚕中间丝腺产生的任何重组蛋白质在茧中分泌并容易地从茧中提取重组蛋白质而不修饰蛋白质构象的新手段，提供了用于表达的表达盒中的多核苷酸通过蚕中间丝腺的重组蛋白质与功能上连接于重组蛋白质结构基因，该多核苷酸由构建丝胶蛋白1或丝胶蛋白2基因的启动子区域的多核苷酸构成，并与其功能连接，构建杆状病毒的同源区。

2. 发明申请

US20070083940A1 Transformed silkworm producing human collagen 审中-公开
标题翻译：转化蚕生产人胶原蛋白
公开(公告)号：US20070083940A1
公开(公告)日：2007-04-12
申请号：US10475107
申请日：2001-06-11
申请人： Katsutoshi Yoshizato , Masahiro Tomita , Tsutomu Satou , Hajime Mori , Toshiki Tamura , Takahiro Adachi , Hiroto Munetsuna
发明人： Katsutoshi Yoshizato , Masahiro Tomita , Tsutomu Satou , Hajime Mori , Toshiki Tamura , Takahiro Adachi , Hiroto Munetsuna
IPC分类号： A01K67/033
CPC分类号： C12N15/8509 , A01K67/0333 , A01K67/0339 , A01K2217/05 , A01K2267/01
摘要： The present invention provides a transformed silkworm which has a polynucleotide encoding human collagen within the genomic DNA and produces recombinant human collagen as a part of proteins in the cocoon or the silk gland; a process for producing recombinant human collagen by using this transformed silkworm; and a recombinant vector for use in the generation of the transformed silkworm. Since human collagen is collected from the cocoon discharged by this transformed silkworm or its silk gland, highly pure human collagen can be conveniently obtained in a large amount. Moreover, because the recombinant human collagen produced by the transformed silkworm is safe collagen which is free from any fear of the contamination with pathogens such as viruses or prions and exhibits no antigenicity toward humans. Thus, it can be utilized in various industrial fields including medicines, foods, cosmetics and the like.
摘要翻译：本发明提供了一种转化的蚕，其具有在基因组DNA内编码人胶原蛋白的多核苷酸，并产生作为茧或丝腺中的蛋白质的一部分的重组人胶原蛋白; 通过使用该转化的蚕生产重组人胶原蛋白的方法; 以及用于产生转化的蚕的重组载体。由于由这种转化的蚕丝或其丝腺排出的茧收集人胶原蛋白，所以可以方便地获得高纯度的人胶原蛋白。此外，因为由转化的蚕产生的重组人胶原是安全的胶原蛋白，它不受任何对病原体（例如病毒或朊病毒）的污染的恐惧，并且对人类没有抗原性。因此，可以用于包括药品，食品，化妆品等在内的各种工业领域。

3. 发明授权

US08278499B2 Nonalcoholic steatohepatitis model rodent and fatty liver model rodent 失效
标题翻译：非酒精性脂肪性肝炎模型啮齿动物和脂肪肝模型啮齿动物
公开(公告)号：US08278499B2
公开(公告)日：2012-10-02
申请号：US12308758
申请日：2007-06-13
申请人： Chise Mukaidani , Katsutoshi Yoshizato , Miho Kataoka
发明人： Chise Mukaidani , Katsutoshi Yoshizato , Miho Kataoka
IPC分类号： A01K67/00 , A01K67/027 , G01N33/00
CPC分类号： A01K67/0271 , A01K2207/12 , A01K2227/105 , A01K2267/035 , A01K2267/0362 , G01N33/5088
摘要： Disclosed is a nonhuman animal showing the symptoms of human nonalcoholic steatohepatitis which is obtained by transplanting human hepatocytes into an immunodeficient hepatopathic nonhuman animal to produce a chimeric nonhuman animal and then transplanting human hepatocytes that are propagated in the body of the chimeric nonhuman animal into an immunodeficient hepatopathic nonhuman animal of the same species as the immunodeficient hepatopathic nonhuman animal described above, as well as a nonhuman animal showing the symptoms of human fatty liver which is obtained by transplanting human hepatocytes into an immunodeficient hepatopathic nonhuman animal to produce a chimeric nonhuman animal.
摘要翻译：公开了一种非人动物，其显示人类非酒精性脂肪性肝炎的症状，其通过将人肝细胞移植到免疫缺陷型肝病非人类动物中以产生嵌合非人动物，然后将在嵌合非人动物体内繁殖的人肝细胞移植到免疫缺陷与上述免疫缺陷性肝病非人类动物相同物种的肝病非人类动物，以及显示通过将人肝细胞移植到免疫缺陷型肝病非人类动物中以产生嵌合非人动物而获得的人类脂肪肝症状的非人动物。

4. 发明申请

US20100197019A1 Method for Cultivation of Hair Follicular Dermal Sheath Cells 有权
标题翻译：毛囊皮肤细胞培养方法
公开(公告)号：US20100197019A1
公开(公告)日：2010-08-05
申请号：US11992753
申请日：2006-10-02
申请人： Koei Toyoshima , Mikaru Matsunaga , Katsutoshi Yoshizato
发明人： Koei Toyoshima , Mikaru Matsunaga , Katsutoshi Yoshizato
IPC分类号： C12N5/02 , C12N5/00
CPC分类号： C12N5/0627 , C12N2501/115 , C12N2501/135
摘要： A method for culture of hair follicular dermal sheath cells or precursor cells thereof which are potent cellular materials for such as hair regeneration by cell transplantation is provided. That is, by performing culture in an animal cell culture medium supplemented with platelet-derived growth factor AA (PDGF-AA) and fibroblast growth factor 2 (FGF2), hair follicular dermal sheath cells are proliferated while sustaining their function, or hair follicular dermal sheath precursor cells are differentiated into dermal sheath cells and proliferated.
摘要翻译：提供了通过细胞移植进行毛发再生的有效细胞材料的毛囊皮肤细胞或其前体细胞的培养方法。也就是说，通过在补充有血小板衍生生长因子AA（PDGF-AA）和成纤维细胞生长因子2（FGF2）的动物细胞培养基中进行培养，毛囊真皮鞘细胞在维持其功能的同时增殖，或毛囊皮肤鞘前体细胞分化成皮肤鞘细胞并增殖。

5. 发明授权

US4883487A Method of producing an artifical skin 失效
标题翻译：生产人造皮肤的方法
公开(公告)号：US4883487A
公开(公告)日：1989-11-28
申请号：US34958
申请日：1987-04-06
申请人： Katsutoshi Yoshizato , Toshio Taira , Teruo Miyata
发明人： Katsutoshi Yoshizato , Toshio Taira , Teruo Miyata
IPC分类号： A61L27/00 , A61F2/00 , A61F2/10 , A61L27/24 , A61L27/60
CPC分类号： A61L27/24 , A61F2/105 , A61L27/60 , A61F2310/00365 , Y10S623/901
摘要： An artifical skin comprising an insoluble atelocollagen sheet and an epidermal cell layer cultured only on one surface of the sheet, and a method of producing the same are disclosed, which method includes the steps of: forming an insoluble atelocollagen sheet; inoculating epidermal cells only on one surface of the atelocollagen sheet; and suspending the atelocollagen sheet in a liquid culture medium to culture the epidermal cells, thereby forming an epidermal cell layer.
摘要翻译：公开了包含不溶性胶原蛋白片和仅在片材的一个表面上培养的表皮细胞层的人造皮肤及其制造方法，该方法包括以下步骤：形成不溶性胶原蛋白片; 仅在表皮细胞上接种atelocollagen片的一个表面; 并将所述胶原蛋白片悬浮在液体培养基中以培养表皮细胞，由此形成表皮细胞层。

6. 发明申请

US20070128172A1 Hair growth method 审中-公开
标题翻译：头发生长法
公开(公告)号：US20070128172A1
公开(公告)日：2007-06-07
申请号：US10581606
申请日：2004-12-03
申请人： Katsutoshi Yoshizato , Takashi Shimada , Koei Toyoshima , Mikaru Matsunaga
发明人： Katsutoshi Yoshizato , Takashi Shimada , Koei Toyoshima , Mikaru Matsunaga
IPC分类号： A01N63/00 , C12N5/00 , C12N5/08 , A01N65/00 , C12N5/02
CPC分类号： A61L27/3869 , A61F2/10 , A61K35/12 , A61L27/362 , A61L27/3666 , A61L27/3813 , A61L2430/18 , C12N5/0627 , C12N2501/115
摘要： A method of enabling effective hair growth and furthermore, inducing hair growth closely similar to the natural hair state in the case of transplanting dermal papillae or cultured dermal papilla cells into the skin to regenerate the hair, characterized by comprising transplanting a composition containing the following components into an epidermis defect site: (a) dermal papillae or dermal papilla cells, (b) an epidermal tissue or epidermal cells and/or (c) a tissue constituting hair follicles or cells thereof.
摘要翻译：此外，在将真皮乳头或培养的真皮乳头细胞移植到皮肤中以再生毛发的情况下，能够有效地进行毛发生长的方法，此外，诱导与天然毛发状态非常相似的毛发生长，其特征在于，包括移植含有以下成分的组合物进入表皮缺损部位：（a）真皮乳头或真皮乳头细胞，（b）表皮组织或表皮细胞和/或（c）构成毛囊或其细胞的组织。

7. 发明申请

US20050119463A1 Antibody recognizing proliferative human liver cells proliferative human liver cells and functional human liver cells 失效
标题翻译：抗体识别增殖性人肝细胞增殖性人肝细胞和功能性人肝细胞
公开(公告)号：US20050119463A1
公开(公告)日：2005-06-02
申请号：US10509096
申请日：2003-03-25
申请人： Chise Mukaidani , Katsutoshi Yoshizato , Chihiro Yamasaki
发明人： Chise Mukaidani , Katsutoshi Yoshizato , Chihiro Yamasaki
IPC分类号： C07K16/18 , C07K16/28 , C12N5/071 , C12N5/18 , C12P21/08 , A61K39/395 , C12N5/08
CPC分类号： C12N5/067 , C07K16/18 , C07K16/28 , C12N5/0697 , C12N2501/38 , C12N2510/00
摘要： The invention provides a monoclonal antibody which specifically recognizes proliferative human hepatocytes, a method for isolating proliferative human hepatocytes by using the antibody, and a method for inducing to differentiate the proliferative human hepatocytes to functional human hepatocytes. Further, the invention provides the proliferative human hepatocytes and functional human hepatocytes obtained by the methods, as well as a cell kit and a hybrid artificial liver comprising those hepatocytes.
摘要翻译：本发明提供特异性识别增殖性人肝细胞的单克隆抗体，通过使用抗体分离增殖性人肝细胞的方法，以及诱导将增殖性人肝细胞分化为功能性人肝细胞的方法。此外，本发明提供了通过所述方法获得的增殖性人肝细胞和功能性人肝细胞，以及包含所述肝细胞的细胞试剂盒和混合人造肝。

8. 发明授权

US6004810A Liver parenchymal cells having clonal growth ability, method for obtaining same, method for subculturing same, and subculturing system of primary hepatocytes 失效
标题翻译：具有克隆生长能力的肝实质细胞，获得其的方法，传代培养方法，原代肝细胞传代培养系统
公开(公告)号：US6004810A
公开(公告)日：1999-12-21
申请号：US419982
申请日：1995-04-11
申请人： Chise Tateno , Katsutoshi Yoshizato
发明人： Chise Tateno , Katsutoshi Yoshizato
IPC分类号： C12N5/074 , C12N5/06
CPC分类号： C12N5/0672 , C12N2500/38 , C12N2501/11
摘要： The present invention provides liver parenchymal cells having a clonal growth ability, which possesses at least one of the cell biological properties such as: presence of peroxysome; being positive to hepatocyte-markers; being partially positive to neoplastic hepatocyte-markers or immature hepatocyte-markers; being positive to antibodies against the surface antigens of ovall cells; and being partially positive to bile duct cell-markers. The present invention also provides a method for obtaining such cells and a method for subculturing such cells.With the liver parenchymal cells above, it will be possible to research in detail the process of development and differentiation, the mechanisms of growth and functional expression of hepatic cells, and to open up a new way to clalification of mechanisms or hepatoma and various other diseases and to development of therapeutic method against these disseases.
摘要翻译：本发明提供具有克隆生长能力的肝实质细胞，其具有至少一种细胞生物学特性，例如：存在过氧化物酶体; 对肝细胞标志阳性; 对肿瘤性肝细胞标志物或未成熟肝细胞标志物部分阳性; 对抗卵巢细胞表面抗原的抗体呈阳性; 并且对胆管细胞标志物部分阳性。本发明还提供了获得这种细胞的方法以及传代这种细胞的方法。随着肝实质细胞的上游，有可能详细研究发育和分化过程，肝细胞生长和功能表达的机制，并开辟新的机制或肝癌和各种其他疾病的分离方式并开发针对这些疾病的治疗方法。

9. 发明授权

US5851831A Method for long term subculture of dermal papilla cells 失效
标题翻译：长期继代培养真皮乳头细胞的方法
公开(公告)号：US5851831A
公开(公告)日：1998-12-22
申请号：US419708
申请日：1995-04-11
申请人： Mutsumi Inamatsu , Takashi Matsuzaki , Katsutoshi Yoshizato
发明人： Mutsumi Inamatsu , Takashi Matsuzaki , Katsutoshi Yoshizato
IPC分类号： C12N5/071 , C12N5/00
CPC分类号： C12N5/0627 , C12N2502/094
摘要： This invention provides a method for culturing dermal papilla cells with at least either of the mammalian epidermal cells from the sole or other portions of a mammal and the conditioned medium thereof, in order to permit long stable subculture of dermal papilla cells while keeping the original function thereof intact.
摘要翻译：本发明提供了用哺乳动物表皮细胞中至少一种哺乳动物表皮细胞培养真皮乳头细胞的方法，该方法来自哺乳动物的其他部分和其条件培养基，以便允许真皮乳头细胞的长期稳定的传代培养，同时保持原始功能它完好无损。

10. 发明授权

US07560279B2 Method of proliferating human hepatocytes and method for obtaining human hepatocytes 失效
标题翻译：增殖人肝细胞的方法和获得人类肝细胞的方法
公开(公告)号：US07560279B2
公开(公告)日：2009-07-14
申请号：US10509032
申请日：2003-03-25
申请人： Chise Mukaidani , Katsutoshi Yoshizato
发明人： Chise Mukaidani , Katsutoshi Yoshizato
IPC分类号： C12N5/08 , C12N5/00 , G01N33/00
CPC分类号： C12N5/067 , C07K16/18 , C07K16/28 , C12N5/0697 , C12N2501/38 , C12N2510/00
摘要： Transplanting human hepatocytes into a liver of an immunodeficient hepatopathy mouse, and then feeding the mouse transplanted with the human hepatocytes under such a condition as being protected from the attack by human complement produced by the human hepatocytes thereby proliferating the transplanted human hepatocytes in the mouse liver. Further, obtaining human hepatocytes in large scale by repeating the above steps using the proliferated human hepatocytes.
摘要翻译：将人肝细胞移植到免疫缺陷型肝病小鼠的肝脏中，然后在被保护免受人肝细胞产生的人补体攻击的条件下喂养移植有人肝细胞的小鼠，从而增殖小鼠肝脏中移植的人肝细胞。此外，通过使用增殖的人肝细胞重复上述步骤，大规模获得人肝细胞。

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