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1. 发明授权

US5783399A Chemiluminescent assay methods and devices for detecting target analytes 失效
标题翻译：用于检测目标分析物的化学发光测定方法和装置
公开(公告)号：US5783399A
公开(公告)日：1998-07-21
申请号：US560094
申请日：1995-11-17
申请人： Mary Ann Childs , Gregory K. Shipman , William P. Trainor , Erick Gray , David Bernstein
发明人： Mary Ann Childs , Gregory K. Shipman , William P. Trainor , Erick Gray , David Bernstein
IPC分类号： C12Q1/66 , G01N21/76 , G01N33/543 , G01N33/53 , C12Q1/00 , C12Q1/02 , G01N33/554
CPC分类号： C12Q1/66 , G01N21/76 , G01N33/54366 , Y10S435/968
摘要： A method which utilize chemiluminescence for analyte detection and the detection of bacteria on surfaces. The method uses a device comprising a sampling portion made of a first adsorbent material, a container, and a second adsorbent material located within the container. The sampling portion collects analytes from a test sample which includes a surface or volume of a liquid. The second adsorbent material holds one or more chemiluminescent components including luciferase enzyme and cofactors in a dry state. In a preferred embodiment, the sampling portion is swabbed over a suspected contaminated surface. A bacteriolytic solution is then added to the adsorbent and releases ATP from any bacteria present. The ATP diffuses into the second adsorbent and mixes with the reagents therein to produce detectable light.
摘要翻译：利用化学发光进行分析物检测和表面细菌检测的方法。该方法使用包括由位于容器内的第一吸附剂材料，容器和第二吸附材料制成的取样部分的装置。采样部分从包括液体的表面或体积的测试样品中收集分析物。第二吸附材料在干燥状态下保持一种或多种化学发光成分，包括荧光素酶和辅因子。在优选实施例中，采样部分被擦拭在可疑的污染表面上。然后将溶菌溶液加入到吸附剂中并从存在的任何细菌释放ATP。 ATP扩散到第二吸附剂中并与其中的试剂混合以产生可检测的光。

2. 发明授权

US5833923A Sampling-assay interface system 失效
标题翻译：取样分析界面系统
公开(公告)号：US5833923A
公开(公告)日：1998-11-10
申请号：US577107
申请日：1995-12-22
申请人： Joseph A. McClintock , Mary Ann Childs , Gregory K. Shipman , William P. Trainor , Erick Gray , David Bernstein
发明人： Joseph A. McClintock , Mary Ann Childs , Gregory K. Shipman , William P. Trainor , Erick Gray , David Bernstein
IPC分类号： G01N21/03 , G01N21/76
CPC分类号： G01N21/76 , G01N21/03
摘要： A system and method for performing an assay for target analytes includes a sampling-device holder interface and a quantifier. The sampling device has a sampling portion and a reading portion containing a chemiluminescent reagent. The interface has a sampling-device holder and a light detector. The holder has a housing having an enclosure with a channel and a base plate with an opening. The base plate and the enclosure houses a slidable tray. The tray has a compartment adapted to seat the sampling device and an opening extending through the compartment. The reading portion is aligned with the tray opening. When the tray is closed, the tray opening is also aligned with the base plate opening, exposing the reading portion. Once the sampling portion is exposed to a sample suspected of containing the target analyte, at least one carrier liquid is introduced, if needed, to the sampling portion to transport any target analyte to the reading portion by dispersion. To measure the emitted light, the sampling device is seated into the tray. The tray is then inserted into housing. The light detector, which is connected in close proximity to the reading portion, outputs a signal. This signal is then converted to quantifiable data indicative of the target analyte quantity, using for instance, an ammeter.
摘要翻译：用于对目标分析物进行测定的系统和方法包括采样设备保持器接口和定量器。采样装置具有采样部分和含有化学发光试剂的读取部分。该接口具有取样装置支架和光检测器。保持器具有壳体，其具有带通道的外壳和具有开口的基板。基板和外壳容纳可滑动的托盘。托盘具有适于安置取样装置的隔室和延伸穿过隔间的开口。阅读部分与托盘开口对齐。当托盘关闭时，托盘开口也与基板开口对准，露出读取部分。一旦采样部分暴露于怀疑含有目标分析物的样品，如果需要，将至少一种载体液体引入采样部分，以通过分散体将任何目标分析物输送到读取部分。为了测量发出的光线，采样装置就位于托盘中。然后将托盘插入外壳。连接在阅读部分附近的光检测器输出信号。然后，使用例如电流表将该信号转换成指示目标分析物量的可量化数据。

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