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1. 发明申请

US20090233296A1 Thiamin production by fermentation 有权
标题翻译：硫胺素通过发酵生产
公开(公告)号：US20090233296A1
公开(公告)日：2009-09-17
申请号：US12380610
申请日：2009-03-02
申请人： Markus G. Goese , John B. Perkins , Ghislain Schyns
发明人： Markus G. Goese , John B. Perkins , Ghislain Schyns
IPC分类号： C12Q1/68 , C12N1/20 , C12N1/21 , C12P17/16 , C07H21/00 , C07H21/04 , C12Q1/48
CPC分类号： C12R1/125 , C12N15/52 , C12P17/167 , C12Q1/04 , C12Q1/18
摘要： The present invention provides a method for producing thiamin products using a microorganism containing a mutation that causes it to overproduce and release thiamin products into the medium. Biologically pure cultures of the microorganisms and isolated polynucleotides containing the mutations are also provided. In addition, methods for detecting a pathogenic microorganism in a clinical sample, assays for identifying an antibiotic, as well as, antibiotics identified by such assays are provided.
摘要翻译：本发明提供使用含有突变的微生物生产硫胺素的方法，所述突变使其过量产生并将硫胺素释放到培养基中。还提供了微生物的生物学纯培养物和含有突变的分离的多核苷酸。此外，提供了用于检测临床样品中的致病微生物的方法，用于鉴定抗生素的测定法以及通过这些测定法鉴定的抗生素。

2. 发明授权

US08329395B2 Thiamin production by fermentation 有权
标题翻译：硫胺素通过发酵生产
公开(公告)号：US08329395B2
公开(公告)日：2012-12-11
申请号：US12380610
申请日：2009-03-02
申请人： Markus G. Goese , John B. Perkins , Ghislain Schyns
发明人： Markus G. Goese , John B. Perkins , Ghislain Schyns
IPC分类号： C12Q1/68 , C12N1/21 , C12P17/16 , C07H21/04
CPC分类号： C12R1/125 , C12N15/52 , C12P17/167 , C12Q1/04 , C12Q1/18
摘要： The present invention provides a method for producing thiamin products using a microorganism containing a mutation that causes it to overproduce and release thiamin products into the medium. Biologically pure cultures of the microorganisms and isolated polynucleotides containing the mutations are also provided. In addition, methods for detecting a pathogenic microorganism in a clinical sample, assays for identifying an antibiotic, as well as, antibiotics identified by such assays are provided.
摘要翻译：本发明提供使用含有突变的微生物生产硫胺素的方法，所述突变使其过量产生并将硫胺素释放到培养基中。还提供了微生物的生物学纯培养物和含有突变的分离的多核苷酸。此外，提供了用于检测临床样品中的致病微生物的方法，用于鉴定抗生素的测定法以及通过这些测定法鉴定的抗生素。

3. 发明授权

US07435571B2 Microbial production of COQ10 有权
标题翻译： COQ10微生物生产
公开(公告)号：US07435571B2
公开(公告)日：2008-10-14
申请号：US10546109
申请日：2004-02-13
申请人： Markus Huembelin , Rual Lopez-Ulibarri , John B. Perkins , Ghislain Schyns
发明人： Markus Huembelin , Rual Lopez-Ulibarri , John B. Perkins , Ghislain Schyns
IPC分类号： C12N9/12 , C12N9/10 , C12N15/00 , C12N1/20 , C07H21/04
CPC分类号： C12P7/66 , C07K2319/00 , C12N9/1085 , C12Y205/01031
摘要： The present Invention relates to enzymes involved in the Synthesis of Coenzyme Q-10, i.e., decaprenyl diphosphate (DPP) synthase and 4-hydroxybenzoate polyprenyltransferase, to isolated DNA encoding said enzymes and to methods for the microbial production of Coenzyme Q-10.
摘要翻译：本发明涉及参与合成辅酶Q-10，即十聚丙酸二磷酸酯（DPP）合成酶和4-羟基苯甲酸酯聚丙烯酰基转移酶的编码所述酶的分离的DNA以及辅酶Q-10的微生物生产方法。

4. 发明申请

US20130216653A1 SPORE SURFACE DISPLAY OF BIOACTIVE MOLECULES 审中-公开
标题翻译：生物活性分子的表面显示
公开(公告)号：US20130216653A1
公开(公告)日：2013-08-22
申请号：US13807804
申请日：2011-06-28
申请人： John B. Perkins , Zoltan Pragai , Ghislain Schyns
发明人： John B. Perkins , Zoltan Pragai , Ghislain Schyns
IPC分类号： C12N11/16 , A23K1/00 , C12N15/74
CPC分类号： C12N11/16 , A23K10/16 , A23K10/18 , A23K20/189 , A23K50/10 , A23K50/75 , C07K14/32 , C12N3/00 , C12N9/16 , C12N15/74 , C12N15/75
摘要： The present invention relates to the display of bioactive molecules at the surface of spores for both in vitro and in vivo applications. Three small open reading frames (ORFs) have been identified which are very useful for display of bioactive molecules at the spore surface. The encoded small proteins have a molecular weight of less than 12 kDa, which corresponds to about less than 100 amino acids.
摘要翻译：本发明涉及在体外和体内应用中在孢子表面显示生物活性分子。已经鉴定了三个小的开放阅读框（ORFs），其对于在孢子表面显示生物活性分子是非常有用的。编码的小蛋白质具有小于12kDa的分子量，其对应于约小于100个氨基酸。

5. 发明授权

US09631185B2 Fusion of bioactive molecules 有权
公开(公告)号：US09631185B2
公开(公告)日：2017-04-25
申请号：US14124769
申请日：2012-06-11
申请人： Ghislain Schyns , Stephane Duval , Aurelie Brelin
发明人： Ghislain Schyns , Stephane Duval , Aurelie Brelin
IPC分类号： C12N9/16 , C07K14/32 , C07K14/335 , A23K20/174 , A23K20/147 , A23K20/189 , A23K20/20 , A23K50/75 , A23K50/30 , A23K50/80 , A23L33/18
CPC分类号： C12N9/16 , A23K20/147 , A23K20/174 , A23K20/189 , A23K20/30 , A23K50/30 , A23K50/75 , A23K50/80 , A23L33/18 , C07K14/32 , C07K14/335 , C07K2319/00 , C12Y301/03008 , C12Y301/03026
摘要： Fusion-proteins containing an enzyme, preferably a feed or food enzyme, coupled to a gut surface-binding domain are presented. The fusion-proteins can be used to promote feed utilization in animals. In a particular example, a Fusion enzyme according to the invention comprising a gut-surface-binding polypeptide segment linked to a phytase show an increased resident time in the gut, which leads to an increased amount of time given to the enzyme to catalyse the corresponding reaction which finally leads to improved feed utilisation.

6. 发明申请

US20140356478A1 FUSION OF BIOACTIVE MOLECULES 有权
标题翻译：生物活性分子的融合
公开(公告)号：US20140356478A1
公开(公告)日：2014-12-04
申请号：US14124769
申请日：2012-06-11
申请人： Ghislain Schyns , Stephane Duval , Aurelie Brelin
发明人： Ghislain Schyns , Stephane Duval , Aurelie Brelin
IPC分类号： C12N9/16 , A23K1/16 , A23K1/165 , C07K14/32 , C07K14/335
CPC分类号： C12N9/16 , A23K20/147 , A23K20/174 , A23K20/189 , A23K20/30 , A23K50/30 , A23K50/75 , A23K50/80 , A23L33/18 , C07K14/32 , C07K14/335 , C07K2319/00 , C12Y301/03008 , C12Y301/03026
摘要： Fusion-proteins containing an enzyme, preferably a feed or food enzyme, coupled to a gut surface-binding domain are presented. The fusion-proteins can be used to promote feed utilization in animals. In a particular example, a Fusion enzyme according to the invention comprising a gut-surface-binding polypeptide segment linked to a phytase show an increased resident time in the gut, which leads to an increased amount of time given to the enzyme to catalyse the corresponding reaction which finally leads to improved feed utilisation.
摘要翻译：提供了含有与肠表面结合域偶联的酶，优选饲料或食物酶的融合蛋白。融合蛋白可用于促进动物的饲料利用。在具体实例中，包含与肌醇六磷酸酶连接的肠表面结合多肽片段的根据本发明的融合酶显示在肠中增加的停留时间，这导致增加的时间量给酶以催化相应的最终导致饲料利用率提高的反应。

7. 发明申请

US20060088920A1 Microbial production of coq10 有权
标题翻译： coq10微生物生产
公开(公告)号：US20060088920A1
公开(公告)日：2006-04-27
申请号：US10546109
申请日：2004-02-13
申请人： Markus Huembelin , Rual Lopez-Ulibarri , John Perkkins , Ghislain Schyns
发明人： Markus Huembelin , Rual Lopez-Ulibarri , John Perkkins , Ghislain Schyns
IPC分类号： C12P7/66 , C07H21/04 , C12P21/06 , C12N9/10 , C12N15/74 , C12N1/21
CPC分类号： C12P7/66 , C07K2319/00 , C12N9/1085 , C12Y205/01031
摘要： The present Invention relates to enzymes involved in the Synthesis of Coenzyme Q-10, i.e., decaprenyl diphosphate (DPP) synthase and 4-hydroxybenzoate polyprenyltransferase, to isolated DNA encoding said enzymes and to methods for the microbial production of Coenzyme Q-10.
摘要翻译：本发明涉及参与合成辅酶Q-10，即十聚丙酸二磷酸酯（DPP）合成酶和4-羟基苯甲酸酯聚丙烯酰基转移酶的编码所述酶的分离的DNA以及辅酶Q-10的微生物生产方法。

8. 发明申请

US20100055244A1 SPORE SURFACE DISPLAYS OF BIOACTIVE MOLECULES 审中-公开
标题翻译：生物活性分子的表面显示
公开(公告)号：US20100055244A1
公开(公告)日：2010-03-04
申请号：US12375976
申请日：2007-08-09
申请人： Adriano O. Henriques , Ghislain Schyns , Thibaut José Wenzel , Sebastian Potot
发明人： Adriano O. Henriques , Ghislain Schyns , Thibaut José Wenzel , Sebastian Potot
IPC分类号： A23K1/165 , C12N1/21 , A23K1/175
CPC分类号： C12N15/75 , A23K10/16 , A23K10/18 , A23K20/189 , A61K38/00 , C07K14/32 , C07K14/33 , C07K14/38 , C12N9/16 , C12Y301/03001 , C12Y301/03008 , C12Y301/03026 , C12Y302/01031
摘要： This invention discloses novel bacterial spore systems. It has now been found surprisingly that under certain conditions bacterial spore systems can be used in the food and feed industry, preferably in animal feeding and as biohybrid material. More precisely applicant has found the following: Genetically modified or genetically engineered viable spore systems expressing bioactive polypeptides, for example bacteriocins and/or enzymatically active feed enzymes, at the spore surface, have a great potential use in animal feeding. Further, it has been found that genetically modified or “genetically engineered inert spore systems expressing affinity ligands or immobilized enzymes at the surface have a great potential use in biocatalysis and in the construction of biocatalytic films. Especially the resistance to harsh chemicals, desiccation, strong pressure, or high temperatures allows the spores to be a potentially valuable tool for the display of bioactive molecules, like biocatalytic enzymes or bioactive feed enzymes that must survive harsh conditions to deliver their full potential. Finally, applicant has found that instead of translational fusions to spore structural genes as it is known from the prior art described above, passenger bioactive polypeptides, as for example enzymes, bacteriocins, affinity ligands, can also be fused to spore-specific surface enzymes, for example to spore specific enzymes as mentioned herein above.
摘要翻译：本发明公开了新颖的细菌孢子体系。现在已经令人惊奇地发现，在某些条件下，细菌孢子体系可以用于食品和饲料工业，优选用于动物饲养和作为生物混合材料。更准确地说，申请人已经发现以下：在孢子表面上表达生物活性多肽（例如细菌素和/或酶促活性进料酶）的基因改造或遗传工程可行的孢子体系在动物饲养中具有巨大的潜在用途。此外，已经发现在表面上表达亲和配体或固定化酶的遗传修饰或“遗传工程惰性孢子体系在生物催化和生物催化膜的构建中具有很大的潜在用途。特别是对苛刻化学品，干燥，强压或高温的抵抗能使孢子成为显示生物活性分子（如生物催化酶或生物活性饲料酶）的潜在有价值的工具，生物活性分子必须经受恶劣条件才能发挥其潜力。最后，申请人已经发现，如从上述现有技术已知的那样，代替融合孢子结构基因的翻译融合，也可以将游离生物活性多肽（例如酶，细菌素，亲和配体）与孢子特异性表面酶融合，例如如上所述孢子特异性酶。

9. 发明申请

US20060127993A1 Thiamin production by fermentation 审中-公开
公开(公告)号：US20060127993A1
公开(公告)日：2006-06-15
申请号：US10559307
申请日：2004-05-27
申请人： Markus Goese , John Perkins , Ghislain Schyns
发明人： Markus Goese , John Perkins , Ghislain Schyns
IPC分类号： C12P17/12 , C12N1/20 , C12N1/21
CPC分类号： C12R1/125 , C12N15/52 , C12P17/167 , C12Q1/04 , C12Q1/18
摘要： The present invention provides a method for producing thiamin products using a microorganism containing a mutation that causes it to overproduce and release thiamin products into the medium. Biologically pure cultures of the microorganisms and isolated polynucleotides containing the mutations are also provided. In addition, methods for detecting a pathogenic microorganism in a clinical sample, assays for identifying an antibiotic, as well as, antibiotics identified by such assays are provided.

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