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1. 发明授权

US5618678A Methods for detecting PDGF agonist or antagonist activity using PDGF .alpha . 失效
标题翻译：使用PDGFα-受体检测PDGF激动剂或拮抗剂活性的方法
公开(公告)号：US5618678A
公开(公告)日：1997-04-08
申请号：US340754
申请日：1994-11-16
申请人： James D. Kelly , Mark J. Murray
发明人： James D. Kelly , Mark J. Murray
IPC分类号： C12N1/19 , C07K14/71 , C12N5/10 , C12N15/09 , C12N15/12 , G01N33/53 , C12Q1/00
CPC分类号： C07K14/71
摘要： Isolated DNA molecules that encode a novel PDGF receptor are disclosed. The receptor binds the AA, AB and BB isoforms of PDGF with high affinity. Cells transfected or transformed with the DNA molecules are also disclosed. The cells can be used within methods for detecting PDGF agonist or antagonist activity in a test compound.
摘要翻译：公开了编码新型PDGF受体的分离的DNA分子。该受体以高亲和力结合PDGF的AA，AB和BB同种型。还公开了用DNA分子转染或转化的细胞。细胞可用于在测试化合物中检测PDGF激动剂或拮抗剂活性的方法中。

2. 发明授权

US4801542A Expression of biologically active PDGF analogs in eucaryotic cells 失效
标题翻译：生物活性PDGF类似物在真核细胞中的表达
公开(公告)号：US4801542A
公开(公告)日：1989-01-31
申请号：US705175
申请日：1985-02-25
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： C12N15/09 , A61K38/00 , C07H21/04 , C07K14/005 , C07K14/15 , C07K14/195 , C07K14/49 , C07K14/52 , C12N1/16 , C12N1/19 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/16 , C12N15/33 , C12N15/81 , C12P21/02 , C12R1/865 , C12P19/00 , C12P21/00
CPC分类号： C07K14/005 , C07K14/49 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N2740/11022 , Y10S930/12 , Y10S930/221
摘要： Biologically active PDGF analogs expressed in eucaryotic cells are disclosed. The analogs are produced by yeast strains transformed with an extrachromosomal element composed of a strong transcriptional promoter directing the expression of a gene which encodes a protein having substantially the same biological activity as PDGF. Suitable genes include the v-sis gene or a derivative of the v-sis gene of simian sarcoma virus or portions thereof, or the human cDNA gene for PDGF or portions thereof. In particular, DNA sequences encoding polypeptides substantially homologous to the B chain of PDGF are preferred. A secretory signal sequence may be provided upstream of the gene, enabling secretion of the gene product from the host cell. Mitogenic activity is one of the biological activities possessed by these PDGF analogs, making them useful in promoting the growth of mammalian cells.

3. 发明授权

US5474982A PDGF analogs and methods of use 失效
公开(公告)号：US5474982A
公开(公告)日：1995-12-12
申请号：US273779
申请日：1994-07-12
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K38/00 , C07K14/15 , C07K14/49 , C12N15/81
CPC分类号： C12N15/81 , C07K14/49 , A61K38/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , Y10S514/97
摘要： Proteins having substantially the same biological activity as PDGF are provided. In one aspect, a protein homodimer having two polypeptide chains is disclosed, each of the chains being a mosaic of amino acid sequences substantially identical to portions of the A- and B-chains of PDGF, the protein being chemotactic or mitogenic for fibroblasts. Therapeutic compositions comprising such proteins in combination with a physiologically acceptable carrier or diluent are also provided. Such therapeutic compositions may be used within methods for enhancing the wound-healing process in warm-blooded animals.

4. 发明授权

US5428010A Biologically active B-chain homodimers 失效
标题翻译：生物活性B链同型二聚体
公开(公告)号：US5428010A
公开(公告)日：1995-06-27
申请号：US852905
申请日：1992-03-18
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K38/00 , C07K14/15 , C07K14/49 , C12N15/81 , A61K38/27
CPC分类号： C07K14/005 , C07K14/49 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N2740/11022
摘要： Dimeric proteins having substantially the same biological activity as PDGF are disclosed. More specifically, the protein may have two substantially identical polypeptide chains, each of the chains being substantially homologous to the B-chain of PDGF. Alternatively, the protein may have two polypeptide chains that are substantially identical to the B-chain of PDGF. In addition, proteins comprising polypeptides that are variants or derivatives of the B-chain of PDGF are also disclosed. Therapeutic compositions containing these proteins and methods for enhancing the wound-healing process in warm- blooded animals are also disclosed.
摘要翻译：公开了具有与PDGF基本相同的生物活性的二聚体蛋白质。更具体地，蛋白质可以具有两个基本相同的多肽链，每个链基本上与PDGF的B链同源。或者，蛋白质可以具有与PDGF的B链基本相同的两条多肽链。此外，还公开了包含作为PDGF的B链的变体或衍生物的多肽的蛋白质。还公开了含有这些蛋白质的治疗组合物和用于增强温血动物中伤口愈合过程的方法。

5. 发明授权

US5187263A Expression of biologically active PDGE analogs in eucaryotic cells 失效
标题翻译：生物活性PDGE类似物在真核细胞中的表达
公开(公告)号：US5187263A
公开(公告)日：1993-02-16
申请号：US818397
申请日：1992-01-02
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K38/00 , C07K14/15 , C07K14/49 , C12N15/62 , C12N15/81
CPC分类号： C07K14/005 , C07K14/49 , C12N15/625 , C12N15/81 , A61K38/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N2740/11022
摘要： Methods for expressing a variety of biologically active PDGF analogs in eucaryotic cells are disclosed. The methods generally comprise introducing into a eucaryotic host cell a DNA construct capable of directing the expression and secretion of biologically active PDGF analogs in eucaryotic cells. The DNA construct contains a transcriptional promoter followed downstream by a suitable DNA sequence. The DNA sequence may encode a protein substantially homologous to the A-chain or the B-chain of PDGF, or a portion thereof, or an A-B heterodimer. In addition, a portion of the DNA sequence may encode at least a portion of the A-chain, while another portion encodes at least a portion of the B-chain of PDGF. Eucaryotic cells transformed with these DNA constructs are also disclosed. Methods of promoting the growth of mammalian cells, comprising incubating the cells with a biologically active PDGF analog expressed by a eucaryotic host cell transformed with such a DNA construct, are also disclosed.
摘要翻译：公开了在真核细胞中表达多种生物活性PDGF类似物的方法。所述方法通常包括向真核宿主细胞中引入能够指导生物活性PDGF类似物在真核细胞中的表达和分泌的DNA构建体。 DNA构建体含有转录启动子，后续是合适的DNA序列。 DNA序列可以编码与PDGF或其一部分或A-B异源二聚体的A链或B链基本同源的蛋白质。此外，DNA序列的一部分可以编码A链的至少一部分，而另一部分编码PDGF的至少一部分B链。还公开了用这些DNA构建体转化的真核细胞。还公开了促进哺乳动物细胞生长的方法，包括用由这种DNA构建体转化的真核宿主细胞表达的生物活性PDGF类似物孵育细胞。

6. 发明授权

US5045633A Expression of biologically active PDGF analogs in eucaryotic cells 失效
标题翻译：生物活性PDGF类似物在真核细胞中的表达
公开(公告)号：US5045633A
公开(公告)日：1991-09-03
申请号：US235984
申请日：1988-08-23
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K38/00 , C07K14/15 , C07K14/49 , C12N15/62 , C12N15/81
CPC分类号： C07K14/005 , C07K14/49 , C12N15/625 , C12N15/81 , A61K38/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N2740/11022
摘要： Biologically active PDGF analogs expressed in eucaryotic cells are disclosed. The analogs are produced by yeast strains transformed with an extrachromosomal element composed of a strong transcriptional promoter directing the expression of a gene which encodes a protein having substantially the same biological activity as PDGF. Suitable genes include the v-sis gene or a derivative of the v-sis gene of simian sarcoma virus or portions thereof, or the human cDNA gene for PDGF or portions thereof. In particular, DNA sequences encoding polypeptides substantially homologous to the B chain of PDGF are preferred. A secretory signal sequence may be provided upstream of the gene, enabling secretion of the gene product from the host cell. Mitogenic activity is one of the biological activites possessed by these PDGF analogs, making them useful in promoting the growth of mammalian cells.
摘要翻译：公开了在真核细胞中表达的生物活性PDGF类似物。类似物由通过引导表达编码具有与PDGF基本相同的生物学活性的蛋白质的基因的强转录启动子组成的染色体外元件转化的酵母菌株产生。合适的基因包括v-sis基因或猿肉瘤病毒的v-sis基因的衍生物或其部分，或用于PDGF或其部分的人cDNA基因。特别地，优选编码与PDGF的B链基本同源的多肽的DNA序列。可以在基因的上游提供分泌信号序列，从而能够从宿主细胞分泌基因产物。有丝分裂活性是这些PDGF类似物拥有的生物活性之一，使其有助于促进哺乳动物细胞的生长。

7. 发明授权

US4845075A Biologically active B-chain homodimers 失效
标题翻译：生物活性B链同型二聚体
公开(公告)号：US4845075A
公开(公告)日：1989-07-04
申请号：US942161
申请日：1986-12-15
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K35/16 , A61K38/00 , C07K14/15 , C07K14/49 , C12N15/18 , C12N15/62 , C12N15/81
CPC分类号： C07K14/005 , A61K35/16 , C07K14/49 , C12N15/625 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N2740/11022 , Y10S930/12 , Y10S930/221
摘要： Dimeric proteins having substantially the same biological activity as PDGF are disclosed. More specifically, the protein may have two substantially identical polypeptide chains, each of the chains being substantially homologous to the B-chain of PDGF. Alternatively, the protein may have two polypeptide chains that are substantially identical to the B-chain of PDGF. In addition, proteins comprising polypeptides that are variants or derivatives of the B-chain of PDGF are also disclosed. Therapeutic compositions containing these proteins and methods for enhancing the wound-healing process in warm-blooded animals are also disclosed.
摘要翻译：公开了具有与PDGF基本相同的生物活性的二聚体蛋白质。更具体地，蛋白质可以具有两个基本相同的多肽链，每个链基本上与PDGF的B链同源。或者，蛋白质可以具有与PDGF的B链基本相同的两条多肽链。此外，还公开了包含作为PDGF的B链的变体或衍生物的多肽的蛋白质。还公开了含有这些蛋白质的治疗组合物和用于增强温血动物中伤口愈合过程的方法。

8. 发明授权

US5516896A Biologically active B-chain homodimers 失效
标题翻译：生物活性B链同型二聚体
公开(公告)号：US5516896A
公开(公告)日：1996-05-14
申请号：US381096
申请日：1989-07-13
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K35/16 , A61K38/00 , C07K14/15 , C07K14/49 , C12N15/18 , C12N15/62 , C12N15/81 , C12N15/79 , A61K38/18
CPC分类号： C07K14/005 , A61K35/16 , C07K14/49 , C12N15/625 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N2740/11022 , Y10S930/12 , Y10S930/221
摘要： Dimeric proteins having substantially the same biological activity as PDGF are disclosed. More specifically, the protein may have two substantially identical polypeptide chains, each of the chains being substantially homologous to the B-chain of PDGF. Alternatively, the protein may have two polypeptide chains that are substantially identical to the B-chain of PDGF. In addition, proteins comprising polypeptides that are variants or derivatives of the B-chain of PDGF are also disclosed. Therapeutic compositions containing these proteins and methods for enhancing the wound-healing process in warm-blooded animals are also disclosed.
摘要翻译：公开了具有与PDGF基本相同的生物活性的二聚体蛋白质。更具体地，蛋白质可以具有两个基本相同的多肽链，每个链基本上与PDGF的B链同源。或者，蛋白质可以具有与PDGF的B链基本相同的两条多肽链。此外，还公开了包含作为PDGF的B链的变体或衍生物的多肽的蛋白质。还公开了含有这些蛋白质的治疗组合物和用于增强温血动物中伤口愈合过程的方法。

9. 发明授权

US5128321A PDGF analogs and methods of use 失效
标题翻译： PDGF类似物和使用方法
公开(公告)号：US5128321A
公开(公告)日：1992-07-07
申请号：US230190
申请日：1988-08-08
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K38/00 , C07K14/15 , C07K14/49 , C12N15/62 , C12N15/81
CPC分类号： C07K14/005 , C07K14/49 , C12N15/625 , C12N15/81 , A61K38/00 , C07K2319/036 , C07K2319/75 , C12N2740/11022 , Y10S514/97
摘要： Proteins having substantially the same biological activity as PDGF are provided. In one aspect, a protein homodimer having two polypeptide chains is disclosed, each of the chains being a mosaic of amino acid sequences substantially identical to portions of the A- and B-chains of PDGF, the protein being chemotactic or mitogenic for fibroblasts. Therapeutic compositions comprising such proteins in combination with a physiologically acceptable carrier or diluent are also provided. Such therapeutic compositions may be used within methods for enhancing the wound-healing process in warm-blooded animals.
摘要翻译：提供了具有与PDGF基本上相同的生物活性的蛋白质。一方面，公开了具有两条多肽链的蛋白质同二聚体，每条链是与PDGF的A链和B链的部分基本相同的氨基酸序列嵌合体，所述蛋白质对于成纤维细胞具有趋化性或促有丝分裂。还提供包含这些蛋白质与生理上可接受的载体或稀释剂组合的治疗组合物。这些治疗组合物可用于在温血动物中增强伤口愈合过程的方法中。

10. 发明授权

US4849407A Biologically active mosaic proteins 失效
标题翻译：生物活性镶嵌蛋白
公开(公告)号：US4849407A
公开(公告)日：1989-07-18
申请号：US941970
申请日：1986-12-15
申请人： Mark J. Murray , James D. Kelly
发明人： Mark J. Murray , James D. Kelly
IPC分类号： A61K38/00 , C07K14/15 , C07K14/49 , C12N15/62 , C12N15/81
CPC分类号： C07K14/005 , C07K14/49 , C12N15/625 , C12N15/81 , A61K38/00 , C07K2319/036 , C07K2319/75 , C12N2740/11022 , Y10S930/12 , Y10S930/221
摘要： Dimeric proteins having substantially the same biological activity as PDGF are disclosed. More specifically, the proteins may have two polypeptide chains, one of the chains being a mosaic of amino acid sequences substantially identical to portions of the A- or B-chains of PDGF, the second of the chains being substantially homologous to either the A-chain or the B-chain of PDGF, the proteins being chemotactic or mitogenic for fibroblasts. Alternatively, each of the two polypeptide chains may be a mosaic of amino acid sequences as described above. Therapeutic compositions containing these proteins and methods for enhancing the wound-healing process in warm-blooded animals are also disclosed.
摘要翻译：公开了具有与PDGF基本相同的生物活性的二聚体蛋白质。更具体地，蛋白质可以具有两条多肽链，其中一条链是与PDGF的A链或B链的部分基本相同的氨基酸序列的嵌合，第二条链基本上与A- 链或PDGF的B链，所述蛋白质对于成纤维细胞具有趋化性或有丝分裂。或者，两个多肽链中的每一个可以是如上所述的氨基酸序列的嵌合体。还公开了含有这些蛋白质的治疗组合物和用于增强温血动物中伤口愈合过程的方法。

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