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1. 发明授权

US07632983B2 Expression of monoclonal antibodies in duckweed 失效
标题翻译：浮萍中单克隆抗体的表达
公开(公告)号：US07632983B2
公开(公告)日：2009-12-15
申请号：US10794615
申请日：2004-03-05
申请人： Lynn F. Dickey , John R. Gasdaska , Kevin M. Cox , Charles G. Peele , David Spencer
发明人： Lynn F. Dickey , John R. Gasdaska , Kevin M. Cox , Charles G. Peele , David Spencer
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C07K14/56 , C07K16/005 , C07K2317/55 , C12N15/8216 , C12N15/8257
摘要： Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium. Methods of producing recombinant monoclonal antibodies having effector function, and antibodies produced by these methods are provided.
摘要翻译：提供了用于从遗传工程化的浮萍中表达和分泌生物活性多肽的方法，核酸序列和转化的浮萍植物或浮萍结核菌培养物。通过修饰编码多肽的表达盒的核苷酸序列来改善浮萍中重组多肽的表达，从而改善浮萍中的表达。通过将生物活性多肽与指导多肽分泌到培养基中的信号肽连接来改善从浮萍中回收生物活性多肽。提供了具有效应子功能的重组单克隆抗体的方法和通过这些方法产生的抗体。

2. 发明授权

US08017836B2 Expression of plasminogen and microplasminogen in duckweed 失效
标题翻译：浮萍中纤维蛋白溶酶原和微量淀粉样蛋白的表达
公开(公告)号：US08017836B2
公开(公告)日：2011-09-13
申请号：US12644640
申请日：2009-12-22
申请人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
发明人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04 , C12N5/10 , C12N15/12 , C12N15/62
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

3. 发明授权

US07659445B2 Expression of plasminogen and microplasminogen in duck weed 失效
标题翻译：在鸭杂草中表达纤溶酶原和微量淀粉样蛋白
公开(公告)号：US07659445B2
公开(公告)日：2010-02-09
申请号：US11056621
申请日：2005-02-11
申请人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
发明人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
IPC分类号： C12N15/82 , C12N15/12 , C12N15/62
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

4. 发明申请

US20110201905A1 Decision support method for casualty treatment using vital sign combinations 审中-公开
标题翻译：使用生命体征组合进行伤员治疗的决策支持方法
公开(公告)号：US20110201905A1
公开(公告)日：2011-08-18
申请号：US12931848
申请日：2011-02-11
申请人： David Spencer
发明人： David Spencer
IPC分类号： A61B5/00
CPC分类号： A61B5/1455 , A61B5/02405 , A61B5/02416 , A61B5/412
摘要： A device and control software and system capable of accepting patient vital sign readings from an oximeter and applying a predetermined decision algorithm that incorporates unique combinations of vital signs and will allow the care giver to continuously monitor and have continuous feedback regarding certain patient vital functions, body trauma status, and immediate treatment needs, such as the patient's likelihood of the onset of shock and the advisability of introducing blood or blood products to the patient.
摘要翻译：一种能够接受来自血氧计的患者生命体征读数的装置和控制软件和系统，并且应用包含生命体征的独特组合的预定决定算法，并且将允许护理人员持续地监测和持续反馈关于某些患者的重要功能，身体创伤状态和即时治疗需要，例如患者发生休克的可能性以及向患者引入血液或血液制品的可行性。

5. 发明授权

US07788870B1 Method and roofing strip for retarding moss, fungi, and algae growth on roofs 有权
标题翻译：用于延缓屋顶上苔藓，真菌和藻类生长的方法和屋顶条
公开(公告)号：US07788870B1
公开(公告)日：2010-09-07
申请号：US12004463
申请日：2007-12-20
申请人： David Spencer
发明人： David Spencer
IPC分类号： E04D1/00
CPC分类号： E04D13/002
摘要： A method for inhibiting growths on shingled roofs, one or more of the strips installed along shingle courses. The strips are preferably formed of copper and have an upper portion formed with a pattern of through holes along the length of the strip and pattern of dimples extending along a lower portion of the length of the strips to increase the duration and extent of contact of rain or dew with the copper metal to increase the release of cupric ions effective in inhibiting growths on the roof.
摘要翻译：一种用于抑制带屋顶上的生长的方法，一个或多个条带沿着木瓦路线安装。条带优选地由铜形成并且具有沿着带的长度形成有通孔图案的上部部分和沿着条带的长度的下部延伸的凹坑的图案，以增加雨的接触的持续时间和程度或露出铜金属，以增加铜离子的释放，有效抑制屋顶上的生长。

6. 发明申请

US20100152770A1 Truncal Tourniquet System & Method 审中-公开
标题翻译：截颈止血带系统与方法
公开(公告)号：US20100152770A1
公开(公告)日：2010-06-17
申请号：US12638519
申请日：2009-12-15
申请人： David Spencer
发明人： David Spencer
IPC分类号： A61B17/00
CPC分类号： A61B17/1325
摘要： A bleed out prevention system and method includes an adjustable length attachment belt assembly which is passed around the body of a human being suspected of having an injured or severed major blood vessel. The adjustable length attachment belt assembly positions a plunger plate assembly over a location where compressive force can be applied to a portion of the major blood vessel which has not been injured or severed. A compressive force application assembly then applies force which reduces the size of the lumen in the major blood vessel thereby restricting or stopping the flow of blood therethrough.
摘要翻译：排泄预防系统和方法包括可调节长度的附接带组件，其围绕怀疑具有受伤或切断的主要血管的人的身体通过。可调节长度的附接带组件将柱塞板组件定位在可以施加压缩力的位置上，该位置处于未受伤或切断的主要血管的一部分。压缩力施加组件然后施加减小主血管内腔尺寸的力，从而限制或阻止血液通过其中。

7. 发明申请

US20060013360A1 High speed materials sorting using X-Ray fluorescence 审中-公开
标题翻译：使用X射线荧光的高速材料分选
公开(公告)号：US20060013360A1
公开(公告)日：2006-01-19
申请号：US11232574
申请日：2005-09-22
申请人： Edward Sommer , Robert Parrish , David Spencer , Charles Roos
发明人： Edward Sommer , Robert Parrish , David Spencer , Charles Roos
IPC分类号： G01N23/223 , G01T1/36
CPC分类号： B07C5/3427 , B07C5/346 , B07C5/365 , G01N23/223 , G01N2223/076
摘要： A system and process for classifying a piece of material of unknown composition at high speeds, where the system connected to a power supply. The piece is irradiated with first x-rays from an x-ray source, causing the piece to fluoresce x-rays. The fluoresced x-rays are detected with an x-ray detector, and the piece of material is classified from the detected fluoresced x-rays. Detecting and classifying may be cumulatively performed in less than one second. An x-ray fluorescence spectrum of the piece of material may be determined from the detected fluoresced x-rays, and the detection of the fluoresced x-rays may be conditioned such that accurate determination of the x-ray fluorescence spectrum is not significantly compromised, slowed or complicated by extraneous x-rays. The piece of material may be classified by recognizing the spectral pattern of the determined x-ray fluorescence spectrum. The piece of material may be flattened prior to irradiation and detection. The x-ray source may irradiate the first x-rays at a high intensity, and the x-ray source may be an x-ray tube.
摘要翻译：一种系统和过程，用于在系统连接到电源的高速下对未知组成的材料进行分类。用X射线源的第一x射线照射该片，使该片发出X射线。用x射线检测器检测荧光的X射线，并将该片材从检测到的荧光X射线分类。检测和分类可能在不到一秒内累积执行。可以从检测到的荧光X射线确定该片材料的X射线荧光光谱，并且可以调节荧光X射线的检测，使得x射线荧光光谱的精确测定不会受到严重影响，由外来X射线减慢或复杂。可以通过识别确定的X射线荧光光谱的光谱图来分类该材料。在照射和检测之前，该片材料可以变平。 x射线源可以以高强度照射第一x射线，并且x射线源可以是X射线管。

8. 发明申请

US20050197968A1 Method and system for using a camera cell phone in transactions 有权
标题翻译：在交易中使用相机手机的方法和系统
公开(公告)号：US20050197968A1
公开(公告)日：2005-09-08
申请号：US11064409
申请日：2005-02-23
申请人： Pardeep Das , David Spencer
发明人： Pardeep Das , David Spencer
IPC分类号： H04K1/00 , H04L9/00 , H04M3/00 , G06F17/60
CPC分类号： G06Q20/382 , G06Q20/3829 , G06Q20/401
摘要： A method facilitating a transaction between a camera-phone user and a business entity. The business entity produces a printed medium indicating a transaction. The printed medium includes a barcode that includes direct contact data for communicating with the business entity. The barcode further includes specific transaction data relating to the transaction in the printed medium. Using the digital camera feature, the user captures a digital image of the barcode. The camera-phone is programmed to decode the digital image to retrieve the direct contact data and the transaction data embedded in the barcode. The camera-phone then automatically initiates a direct communication with the business entity via the mobile communications device using the decoded direct contact data. One communications are established, the camera phone further transmits the transaction data derived from the barcode to further facilitate the transaction.
摘要翻译：一种促进照相机电话用户与商业实体之间交易的方法。业务实体生成指示交易的打印介质。打印介质包括条形码，其包括用于与业务实体通信的直接联系人数据。条形码还包括与打印介质中的交易有关的特定交易数据。使用数码相机功能，用户捕获条形码的数字图像。相机手机被编程为对数字图像进行解码，以检索直接联系人数据和嵌入在条形码中的交易数据。然后，相机电话使用解码的直接联系人数据通过移动通信设备自动发起与业务实体的直接通信。建立一个通信，相机手机进一步发送从条形码导出的交易数据，以进一步促进交易。

9. 发明申请

US20050048956A1 Method and system for providing directions to a mobile phone user 审中-公开
标题翻译：用于向移动电话用户提供指示的方法和系统
公开(公告)号：US20050048956A1
公开(公告)日：2005-03-03
申请号：US10654339
申请日：2003-09-02
申请人： Pradeep Das , David Spencer
发明人： Pradeep Das , David Spencer
IPC分类号： H04W64/00 , H04Q7/20
CPC分类号： H04W64/00
摘要： A method and system for providing services to a mobile phone user, who needs directions to a desired destination. When the user calls a call center, the call center obtains the location of the mobile phone user from the communications network using a triangulation method. Based on the user's location, the operator at the call center verbally responds to the user with appropriate directions. Advantageously, the service is provided to the brand loyal customers who wish to locate a specific retail establishment such as a gas station or restaurant.
摘要翻译：一种用于向移动电话用户提供服务的方法和系统，所述移动电话用户需要到所需目的地的方向。当用户呼叫呼叫中心时，呼叫中心使用三角测量方法从通信网络获取移动电话用户的位置。根据用户的位置，呼叫中心的运营商以适当的方向口头回复用户。有利地，将服务提供给希望定位诸如加油站或餐馆的特定零售店的品牌忠实客户。

10. 发明授权

US08310211B1 Auto-regulated motion power system 有权
标题翻译：自动调节动力系统
公开(公告)号：US08310211B1
公开(公告)日：2012-11-13
申请号：US12969924
申请日：2010-12-16
申请人： Robert Mount , David Spencer
发明人： Robert Mount , David Spencer
IPC分类号： H02P9/00
CPC分类号： H02P9/48
摘要： An auto-regulated motion power system apparatus that may be used to generate usable electricity from disparate energy sources includes a combination of a variable frequency alternator driven by a primary mover and coupled to a load. An energy control module includes a closed-loop feedback system coupled to a pulse width modulation controller and a switch mode rectifier. The alternator has a modulated control signal input having the ability to vary in frequency and voltage with an output having a controlled voltage and varying frequency. The switch mode rectifier accepts variable AC voltages from the alternator and outputs a constant predetermined DC voltage both to one side of the windings of the alternator and to a first side of a high frequency switch. An output on the pulse width modulation generator is connected to a switch control so that when the switch is closed, current flows through the windings of the alternator and when the switch is open, no current flows through the windings. This modulation of current flow (or lack thereof) regulates the magnetic field strength inside the alternator to produce a stable voltage over a wide range of RPMs.
摘要翻译：可用于从不同能量源产生可用电力的自动调节运动电力系统装置包括由主动臂驱动并耦合到负载的变频交流发电机的组合。能量控制模块包括耦合到脉宽调制控制器和开关模式整流器的闭环反馈系统。交流发电机具有调制控制信号输入，其具有在具有受控电压和变化频率的输出端上频率和电压变化的能力。开关模式整流器接受来自交流发电机的可变交流电压，并向交流发电机的绕组的一侧和高频开关的第一侧输出恒定的预定直流电压。脉冲宽度调制发生器的输出端与开关控制相连，当开关闭合时，电流流过交流发电机的绕组，当开关断开时，电流不会流过绕组。电流（或其不足）的这种调制调节交流发电机内的磁场强度，以在宽的RPM范围内产生稳定的电压。

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