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1. 发明申请

US20100043099A1 EXPRESSION CONTROL ELEMENTS FROM THE LEMNACEAE FAMILY 失效
标题翻译：来自LEMNACEAE FAMILY的表达控制元素
公开(公告)号：US20100043099A1
公开(公告)日：2010-02-18
申请号：US12581752
申请日：2009-10-19
申请人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
发明人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N15/63 , C12N5/10
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/2442 , C12N9/88 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12Y302/01014 , C12Y401/01039
摘要： Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
摘要翻译：提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。组合物包括新的核酸分子及其变体和片段，用于从莲子科泛素，r-组蛋白和几丁质酶基因分离的表达控制元件。还提供了使用本文公开的表达控制元件在植物中表达目标核苷酸序列的方法。该方法包括向植物或植物细胞或结节引入包含与感兴趣的核苷酸序列可操作地连接的本发明的表达控制元件的表达构建体。特别地，组合物和方法可用于增强浮萍中感兴趣的核苷酸序列的表达。还提供了一种新颖的莲子科信号肽编码序列和由其编码的信号肽。当本发明的表达构建体设计用于表达感兴趣的多肽时，该新型信号肽编码序列可以包括在本发明的表达构建体内，以提供所编码的目标多肽的细胞外分泌。

2. 发明授权

US07884264B2 Compositions and methods for inhibition of fucosyltransferase and xylosyltransferase expression in duckweed plants 失效
标题翻译：在浮萍植物中抑制岩藻糖基转移酶和木糖基转移酶表达的组合物和方法
公开(公告)号：US07884264B2
公开(公告)日：2011-02-08
申请号：US11624164
申请日：2007-01-17
申请人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
发明人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
IPC分类号： C12N15/82 , A01H5/00 , C07H21/04
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. In some embodiments, the methods comprise introducing into a duckweed plant a recombinant RNAi construct that provides for the inhibition of expression of α1,3-fucosyltransferase (FucT) and β1,2-xylosyltransferase (XylT). Use of these RNAi constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a “humanized” N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants, including duckweed plants, having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the G0 glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。在一些实施方案中，所述方法包括向浮萍植物中引入提供抑制α1,3-岩藻糖基转移酶（FucT）和“1,2-木糖基转移酶（XylT）”的表达的重组RNAi构建体。这些RNAi构建体用于抑制或抑制这两种酶及其同种型的表达有利地提供具有“人源化”N-糖基化模式的内源和异源蛋白质的生产而不影响植物的生长和发育。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物，包括浮萍植物。还提供了具有基本上均匀的糖基化曲线并且对于G0糖型基本均匀的糖蛋白组合物，包括单克隆抗体组合物。

3. 发明授权

US07632983B2 Expression of monoclonal antibodies in duckweed 失效
标题翻译：浮萍中单克隆抗体的表达
公开(公告)号：US07632983B2
公开(公告)日：2009-12-15
申请号：US10794615
申请日：2004-03-05
申请人： Lynn F. Dickey , John R. Gasdaska , Kevin M. Cox , Charles G. Peele , David Spencer
发明人： Lynn F. Dickey , John R. Gasdaska , Kevin M. Cox , Charles G. Peele , David Spencer
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C07K14/56 , C07K16/005 , C07K2317/55 , C12N15/8216 , C12N15/8257
摘要： Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium. Methods of producing recombinant monoclonal antibodies having effector function, and antibodies produced by these methods are provided.
摘要翻译：提供了用于从遗传工程化的浮萍中表达和分泌生物活性多肽的方法，核酸序列和转化的浮萍植物或浮萍结核菌培养物。通过修饰编码多肽的表达盒的核苷酸序列来改善浮萍中重组多肽的表达，从而改善浮萍中的表达。通过将生物活性多肽与指导多肽分泌到培养基中的信号肽连接来改善从浮萍中回收生物活性多肽。提供了具有效应子功能的重组单克隆抗体的方法和通过这些方法产生的抗体。

4. 发明申请

US20090175886A1 MONOCLONAL ANTIBODIES AGAINST CD30 LACKING IN FUCOSYL AND XYLOSYL RESIDUES 审中-公开
标题翻译：针对FOSOSYL和XYLOSYL残留物中CD30缺失的单克隆抗体
公开(公告)号：US20090175886A1
公开(公告)日：2009-07-09
申请号：US12160990
申请日：2007-01-17
申请人： Amelia Naucy Black , David B. Passmore , Mohan Srinivasan , Lynn F. Dickey , Kevin M. Cox , Charles G. Peele , Ming-Bo Wang
发明人： Amelia Naucy Black , David B. Passmore , Mohan Srinivasan , Lynn F. Dickey , Kevin M. Cox , Charles G. Peele , Ming-Bo Wang
IPC分类号： C07K16/46 , C12N5/04 , C12N5/06 , A61K39/395 , A61P35/00
CPC分类号： C07K16/2878 , A61K2039/505 , C07K2317/13 , C07K2317/21 , C07K2317/41 , C07K2317/72 , C07K2317/732
摘要： The invention pertains to anti-CD30 antibodies that lack fucosyl and xylosyl residues. The antibodies of the invention exhibit increased antibody-dependent cellular cytotoxicity (ADCC) activity, including the ability to lyse CD30-expressing cell lines that are not lysed by the fucosylated and xylosylated form of the antibodies. The invention also provides host cells that express the anti-CD30 antibodies that lack fucosyl and xylosyl residues, wherein the host cells are deficient for a fucosyltransferase and a xylosyltransferase. Methods of using the antibodies to inhibit the growth of CD30 cells, such as tumor cells, are also provided.
摘要翻译：本发明涉及缺乏岩藻糖基和木糖基残基的抗-CD30抗体。本发明的抗体表现出增加的抗体依赖性细胞毒性（ADCC）活性，包括裂解不被岩藻糖基化和木糖基化形式的抗体裂解的表达CD30的细胞系的能力。本发明还提供表达缺乏岩藻糖基和木糖基残基的抗CD30抗体的宿主细胞，其中所述宿主细胞对于岩藻糖基转移酶和木糖基转移酶是缺陷的。还提供了使用抗体抑制CD30细胞如肿瘤细胞生长的方法。

5. 发明授权

US08034916B2 Expression control elements from the Lemnaceae family 失效
标题翻译：来自Lemnaceae科的表达控制元件
公开(公告)号：US08034916B2
公开(公告)日：2011-10-11
申请号：US12581752
申请日：2009-10-19
申请人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
发明人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
IPC分类号： C07H21/04 , A01H5/00 , C12N15/05 , C12N15/82
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/2442 , C12N9/88 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12Y302/01014 , C12Y401/01039
摘要： Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
摘要翻译：提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。组合物包括新的核酸分子及其变体和片段，用于从莲子科泛素，r-组蛋白和几丁质酶基因分离的表达控制元件。还提供了使用本文公开的表达控制元件在植物中表达目标核苷酸序列的方法。该方法包括向植物或植物细胞或结节引入包含与感兴趣的核苷酸序列可操作地连接的本发明的表达控制元件的表达构建体。特别地，组合物和方法可用于增强浮萍中感兴趣的核苷酸序列的表达。还提供了一种新颖的莲子科信号肽编码序列和由其编码的信号肽。当本发明的表达构建体设计用于表达感兴趣的多肽时，该新型信号肽编码序列可以包括在本发明的表达构建体内，以提供所编码的目标多肽的细胞外分泌。

6. 发明授权

US07622573B2 Expression control elements from the lemnaceae family 失效
标题翻译：来自lemnaceae家族的表达控制元件
公开(公告)号：US07622573B2
公开(公告)日：2009-11-24
申请号：US11653593
申请日：2007-01-16
申请人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
发明人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele
IPC分类号： C12N15/82 , C12N5/04 , C07H21/04 , A01H5/04
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/2442 , C12N9/88 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12Y302/01014 , C12Y401/01039
摘要： Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
摘要翻译：提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。组合物包括新的核酸分子及其变体和片段，用于从莲子科泛素，r-组蛋白和几丁质酶基因分离的表达控制元件。还提供了使用本文公开的表达控制元件在植物中表达目标核苷酸序列的方法。该方法包括向植物或植物细胞或结节引入包含与感兴趣的核苷酸序列可操作地连接的本发明的表达控制元件的表达构建体。特别地，组合物和方法可用于增强浮萍中感兴趣的核苷酸序列的表达。还提供了一种新颖的莲子科信号肽编码序列和由其编码的信号肽。当本发明的表达构建体设计用于表达感兴趣的多肽时，该新型信号肽编码序列可以包括在本发明的表达构建体内，以提供所编码的目标多肽的细胞外分泌。

7. 发明授权

US08716557B2 Compositions and methods for inhibition of fucosyltransferase and xylosyltransferase expression in plants 失效
标题翻译：用于抑制植物中岩藻糖基转移酶和木糖基转移酶表达的组合物和方法
公开(公告)号：US08716557B2
公开(公告)日：2014-05-06
申请号：US11624158
申请日：2007-01-17
申请人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele , Ming-Bo Wang
发明人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele , Ming-Bo Wang
IPC分类号： C12N15/82 , C07H21/04 , A01H5/00
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant RNAi construct that provides for the inhibition of expression of α1,3-fucosyltransferase (FucT) and β1,2-xylosyltransferase (XylT) in a plant. Use of these RNAi constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a “humanized” N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the G0 glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。所述方法包括将植物中的α1,3-岩藻糖基转移酶（FucT）和β-木糖基转移酶（XylT）的表达抑制的重组RNAi构建体引入植物中。这些RNAi构建体用于抑制或抑制这两种酶及其同种型的表达有利地提供具有“人源化”N-糖基化模式的内源和异源蛋白质的生产而不影响植物的生长和发育。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物。还提供了具有基本上均匀的糖基化曲线并且对于G0糖型基本均匀的糖蛋白组合物，包括单克隆抗体组合物。

8. 发明授权

US08017836B2 Expression of plasminogen and microplasminogen in duckweed 失效
标题翻译：浮萍中纤维蛋白溶酶原和微量淀粉样蛋白的表达
公开(公告)号：US08017836B2
公开(公告)日：2011-09-13
申请号：US12644640
申请日：2009-12-22
申请人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
发明人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04 , C12N5/10 , C12N15/12 , C12N15/62
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

9. 发明申请

US20100186126A1 EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED 失效
标题翻译：表达胰岛素和微管蛋白在DUCKWEED中的表达
公开(公告)号：US20100186126A1
公开(公告)日：2010-07-22
申请号：US12644640
申请日：2009-12-22
申请人： DAVID SPENCER , LYNN F. DICKEY , JOHN R. GASDASKA , XIAOWEI WANG , KEVIN M. COX , CHARLES G. PEELE
发明人： DAVID SPENCER , LYNN F. DICKEY , JOHN R. GASDASKA , XIAOWEI WANG , KEVIN M. COX , CHARLES G. PEELE
IPC分类号： A01H5/00 , C12N5/10 , C12N9/68
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可以用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

10. 发明申请

US20120276086A1 MONOCLONAL ANTIBODIES AGAINST CD30 LACKING IN FUCOSYL AND XYLOSYL RESIDUES 审中-公开
标题翻译：针对FOSOSYL和XYLOSYL残留物中CD30缺失的单克隆抗体
公开(公告)号：US20120276086A1
公开(公告)日：2012-11-01
申请号：US13449417
申请日：2012-04-18
申请人： Amelia Nancy BLACK , David B. PASSMORE , Mohan SRINIVASAN , Lynn F. DICKEY , Kevin M. COX , Charles G. PEELE , Ming-Bo WANG
发明人： Amelia Nancy BLACK , David B. PASSMORE , Mohan SRINIVASAN , Lynn F. DICKEY , Kevin M. COX , Charles G. PEELE , Ming-Bo WANG
IPC分类号： C07K16/28 , C12N5/071 , A61P37/04 , A61K35/00 , A61P35/02 , C12N5/10 , A61K39/395
CPC分类号： C07K16/2878 , A61K2039/505 , C07K2317/13 , C07K2317/21 , C07K2317/41 , C07K2317/72 , C07K2317/732
摘要： The invention pertains to anti-CD30 antibodies that lack fucosyl and xylosyl residues. The antibodies of the invention exhibit increased antibody-dependent cellular cytotoxicity (ADCC) activity, including the ability to lyse CD30-expressing cell lines that are not lysed by the fucosylated and xylosylated form of the antibodies. The invention also provides host cells that express the anti-CD30 antibodies that lack fucosyl and xylosyl residues, wherein the host cells are deficient for a fucosyltransferase and a xylosyltransferase. Methods of using the antibodies to inhibit the grown of CD30+ cells, such as tumor cells, are also provided.
摘要翻译：本发明涉及缺乏岩藻糖基和木糖基残基的抗-CD30抗体。本发明的抗体表现出增加的抗体依赖性细胞毒性（ADCC）活性，包括裂解不被岩藻糖基化和木糖基化形式的抗体裂解的表达CD30的细胞系的能力。本发明还提供表达缺乏岩藻糖基和木糖基残基的抗CD30抗体的宿主细胞，其中所述宿主细胞对于岩藻糖基转移酶和木糖基转移酶是缺陷的。还提供了使用抗体抑制生长的CD30 +细胞例如肿瘤细胞的方法。

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