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    • 1. 发明申请
    • DISEASE-INDUCIBLE PROMOTERS
    • 疾病诱导促进剂
    • US20090151015A1
    • 2009-06-11
    • US12298134
    • 2007-04-23
    • Luc AdamT. Lynne ReuberKaren S. Century
    • Luc AdamT. Lynne ReuberKaren S. Century
    • A01H1/02C07H21/04C12N15/82A01H5/10A01H5/00
    • C12Q1/6895C12N15/8239C12N15/8282
    • Disease-inducible promoter sequences have been identified that may be used to produce transgenic plants that are both more resistant to disease than control plants, and are wild-type or nearly wild type in appearance. Any of these disease-inducible promoters may be incorporated into expression vectors that each comprise a defense response protein operably linked to the promoter. The expression vectors can be introduced into plants and the defense response protein then ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, in some cases, to more than one type of pathogen, and yet are similar to wild type plants in their morphology and development.
    • 已经鉴定了可以用于产生对照植物对疾病具有更高抗性的转基因植物的疾病诱导型启动子序列,并且在外观上是野生型或近似野生型。 这些疾病诱导型启动子中的任何一种可以并入表达载体中,每个包含与启动子可操作地连接的防御反应蛋白。 表达载体可以引入植物,然后外源表达的防御反应蛋白。 已经显示用许多这些表达载体转化的转基因植物对疾病具有更强的抗性,在某些情况下,对一种以上类型的病原体更具抗性,而且在其形态和发育中与野生型植物相似。
    • 4. 发明授权
    • Disease-inducible promoters
    • 疾病诱导型启动子
    • US07994394B2
    • 2011-08-09
    • US12298134
    • 2007-04-23
    • Luc AdamT. Lynne ReuberKaren S. Century
    • Luc AdamT. Lynne ReuberKaren S. Century
    • A01H5/00A01H5/10C12N15/09C12N15/82
    • C12Q1/6895C12N15/8239C12N15/8282
    • Disease-inducible promoter sequences have been identified that may be used to produce transgenic plants that are both more resistant to disease than control plants, and are wild-type or nearly wild type in appearance. Any of these disease-inducible promoters may be incorporated into expression vectors that each comprise a defense response protein operably linked to the promoter. The expression vectors can be introduced into plants and the defense response protein then ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, in some cases, to more than one type of pathogen, and yet are similar to wild type plants in their morphology and development.
    • 已经鉴定了可以用于产生对照植物对疾病具有更高抗性的转基因植物的疾病诱导型启动子序列,并且在外观上是野生型或近似野生型。 这些疾病诱导型启动子中的任何一种可以并入表达载体中,每个包含与启动子可操作地连接的防御反应蛋白。 表达载体可以引入植物,然后外源表达的防御反应蛋白。 已经显示用许多这些表达载体转化的转基因植物对疾病具有更强的抗性,在某些情况下,对一种以上类型的病原体更具抗性,而且在其形态和发育中与野生型植物相似。
    • 6. 发明申请
    • STRONG ACTIVATION DOMAIN
    • 强活动域
    • US20110126326A1
    • 2011-05-26
    • US13000488
    • 2009-06-26
    • Shiv TiwariRoger CanalesT. Lynne ReuberKaren S. CenturyOliver Ratcliffe
    • Shiv TiwariRoger CanalesT. Lynne ReuberKaren S. CenturyOliver Ratcliffe
    • A01H5/00C07K7/06C07K7/08C07K14/00C07H21/00C12N5/10C12N15/82
    • A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.
    • 从拟南芥蛋白质乙烯响应因子98(AtERF98)中鉴定出新的和强的转录激活结构域。 该领域已经被指定为“EDLL结构域”,并且具有许多高度保守的氨基酸残基,其在来自植物的AtERF98家族的整个成员中发现,包括单子叶植物和雌蕊直系同源物。 当EDLL结构域与植物和酵母的转录因子融合时,显示其具有高度的活性,并且还显示具有与广泛使用的衍生自单纯疱疹的VP16活化区域相当的活化潜力。 当通过序列特异性DNA结合蛋白或通过蛋白质 - 蛋白质相互作用靶向基因启动子时,EDLL结构域也是活性的。 与其他已知的活化结构域(如VP16和GAL4)不同,EDLL结构域的大小相对较小,并且是植物来源的,因此作为在转基因食品作物中应用的强转录激活工具是有利的。