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    • 5. 发明授权
    • Compositions and methods for the modification of physiological responses in plants
    • 用于修饰植物生理反应的组合物和方法
    • US08168860B2
    • 2012-05-01
    • US12209501
    • 2008-09-12
    • Jeffrey L. RosichanDaniel R. Gallie
    • Jeffrey L. RosichanDaniel R. Gallie
    • A01H5/00A01H1/00C12N15/82
    • C12N15/8217C12N15/8237C12N15/8238C12N15/8249C12N15/8266C12N15/8291
    • A gene expression system for controllable expression of ethylene response in a plant cell includes an activation cassette comprising a DNA-binding domain that recognizes a response element; an ecdysone receptor ligand binding domain; and an activation domain; and a target cassette comprising an inducible promoter, which comprises, in operative association, the response element and a minimal promoter responsive to the activation domain. The inducible promoter controls the expression of a nucleic acid sequence that encodes a selected protein that modifies sensitivity to ethylene in the plant. Interaction among the components of the activation cassette and target cassette, when in a plant cell, in the presence of an inducing composition, modulates expression of the selected protein and selectively modulates ethylene sensitivity in the plant cell. This modulation in the expression of the protein is controlled by the timing, the concentration and the duration of the application of the inducing composition. Transgenic plant cells, tissues, organs and entire plants are provided, which in the presence of the inducing composition control ethylene sensitivity. Ethylene sensitivity and/or ethylene production in such transgenic plants and tissues may be controlled for purposes of manipulating ripening, flower senescence and other ethylene sensitive functions of the plant.
    • 用于在植物细胞中可控表达乙烯应答的基因表达系统包括活化盒,其包含识别响应元件的DNA结合结构域; 蜕皮激素受体配体结合域; 和激活域; 以及包含可诱导启动子的靶盒,其包含有效关联的响应元件和响应于激活结构域的最小启动子。 诱导型启动子控制编码选择的蛋白质的核酸序列的表达,其修饰植物中对乙烯的敏感性。 当在植物细胞中存在诱导组合物时,活化盒和靶盒的组分之间的相互作用调节所选蛋白质的表达并选择性调节植物细胞中的乙烯敏感性。 蛋白质表达中的这种调节受诱导组合物施用的时间,浓度和持续时间的控制。 提供转基因植物细胞,组织,器官和整个植物,其在诱导组合物存在下控制乙烯敏感性。 可以控制这些转基因植物和组织中的乙烯敏感性和/或乙烯产生,用于操作植物的成熟,花衰老和其它乙烯敏感功能。
    • 8. 发明申请
    • REGULATING THE ETHYLENE RESPONSE OF A PLANT BY MODULATION OF F-BOX PROTEINS
    • 通过调节F-BOX蛋白调节植物的乙烯反应
    • US20110131688A1
    • 2011-06-02
    • US13015333
    • 2011-01-27
    • Hongwei GuoJoseph R. Ecker
    • Hongwei GuoJoseph R. Ecker
    • A01H5/00
    • C12N15/8291C12N15/8249C12N15/8261C12N15/8266C12N15/8267C12N15/8271
    • The relationship between F-box proteins and proteins involved in the ethylene response in plants is described. In particular, F-box proteins may bind to proteins involved in the ethylene response and target them for degradation by the ubiquitin/proteasome pathway. The transcription factor EIN3 is a key transcription factor mediating ethylene-regulated gene expression and morphological responses. EIN3 is degraded through a ubiquitin/proteasome pathway mediated by F-box proteins EBF1 and EBF2. The link between F-box proteins and the ethylene response is a key step in modulating or regulating the response of a plant to ethylene. Described herein are transgenic plants having an altered sensitivity to ethylene, and methods for making transgenic plant having an altered sensitivity to ethylene by modulating the level of activity of F-box proteins. Methods of altering the ethylene response in a plant by modulating the activity or expression of an F-box protein are described. Also described are methods of identifying compounds that modulate the ethylene response in plants by modulating the level of F-box protein expression or activity.
    • 描述了F-box蛋白和参与植物乙烯应答的蛋白质之间的关系。 特别地,F盒蛋白可能与参与乙烯应答的蛋白结合,并将其靶向通过泛素/蛋白酶体途径降解。 转录因子EIN3是介导乙烯调节基因表达和形态学反应的关键转录因子。 EIN3通过F-box蛋白EBF1和EBF2介导的泛素/蛋白酶体途径降解。 F-box蛋白与乙烯反应之间的联系是调节或调节植物对乙烯的反应的关键步骤。 本文描述了对乙烯具有改变的敏感性的转基因植物,以及通过调节F-box蛋白的活性水平来制备对乙烯具有改变的敏感性的转基因植物的方法。 描述了通过调节F盒蛋白的活性或表达来改变植物中的乙烯应答的方法。 还描述了通过调节F-box蛋白表达或活性的水平鉴定调节植物中乙烯应答的化合物的方法。